Objective: to investigate the effect of two different ways of elevation on limb swelling of closed tibiofibular fractures. Methods: choose from January 2019 to December bone fractures hospital subject in hospital ward...Objective: to investigate the effect of two different ways of elevation on limb swelling of closed tibiofibular fractures. Methods: choose from January 2019 to December bone fractures hospital subject in hospital ward in Guangxi during the period of 60 patients with fracture of tibiofibula closed as the research object, they were randomly divided into control group and research group, 30 cases each, and each line continuous push up limb, intermittent drive up limb. The degree of pain and swelling before and after nursing were evaluated and compared between 2 groups. Results: before nursing, there were no significant differences in VAS score and swelling degree between the two groups (P > 0 0.05). After 2 weeks of nursing, VSA score and swelling degree in observation group were lower than those in control group (P < 0.05). Conclusion: the intermittent elevation of the affected limb for the patients with closed tibiofibular fracture can effectively relieve the pain and swelling degree of the limb. This method is superior to the traditional continuous elevation of the affected limb, and is conducive to the early recovery of the patient's condition, and has clinical promotion value.展开更多
目的:研究在非小细胞肺癌及癌旁组织中ceacam1通过选择性拼接而产生的两种转录产物的调控机制。方法:将用PCR方法获得ceacam1基因中从内含子5至外显子8长1 606 bp DNA片段插入到真核表达载体pCMV中,构建成ceacam1迷你基因模型并与ptb基...目的:研究在非小细胞肺癌及癌旁组织中ceacam1通过选择性拼接而产生的两种转录产物的调控机制。方法:将用PCR方法获得ceacam1基因中从内含子5至外显子8长1 606 bp DNA片段插入到真核表达载体pCMV中,构建成ceacam1迷你基因模型并与ptb基因共转染,用PCR法鉴定转染后的产物变化;根据外显子7序列设计的探针GAE(16-nt)及ACE(8-nt)进行凝胶阻滞分析实验。分离与探针结合的蛋白并进行质谱分析。结果:ptb3种cDNA与ceacam1迷你基因共转染后,CEACAM lL表达水平下降,其中ptb4对迷你基因的表达产物影响最大。仅转染迷你基因的细胞中ceacam1L在两条带中所占比例为76.7%,而与ptb3种重组质粒共转染后,比例分别下降至58.3%、64.8%和54.0%。凝胶阻滞实验表明,探针GAE能与核蛋白结合,而ACE基本不能与核蛋白结合,与GAE结合的蛋白经质谱分析为PTB。结论:PTB过表达与ceacam1低表达有明显的相关性,拼接因子PTB参与ceacam1的选择性拼接。展开更多
文摘Objective: to investigate the effect of two different ways of elevation on limb swelling of closed tibiofibular fractures. Methods: choose from January 2019 to December bone fractures hospital subject in hospital ward in Guangxi during the period of 60 patients with fracture of tibiofibula closed as the research object, they were randomly divided into control group and research group, 30 cases each, and each line continuous push up limb, intermittent drive up limb. The degree of pain and swelling before and after nursing were evaluated and compared between 2 groups. Results: before nursing, there were no significant differences in VAS score and swelling degree between the two groups (P > 0 0.05). After 2 weeks of nursing, VSA score and swelling degree in observation group were lower than those in control group (P < 0.05). Conclusion: the intermittent elevation of the affected limb for the patients with closed tibiofibular fracture can effectively relieve the pain and swelling degree of the limb. This method is superior to the traditional continuous elevation of the affected limb, and is conducive to the early recovery of the patient's condition, and has clinical promotion value.
文摘目的:研究在非小细胞肺癌及癌旁组织中ceacam1通过选择性拼接而产生的两种转录产物的调控机制。方法:将用PCR方法获得ceacam1基因中从内含子5至外显子8长1 606 bp DNA片段插入到真核表达载体pCMV中,构建成ceacam1迷你基因模型并与ptb基因共转染,用PCR法鉴定转染后的产物变化;根据外显子7序列设计的探针GAE(16-nt)及ACE(8-nt)进行凝胶阻滞分析实验。分离与探针结合的蛋白并进行质谱分析。结果:ptb3种cDNA与ceacam1迷你基因共转染后,CEACAM lL表达水平下降,其中ptb4对迷你基因的表达产物影响最大。仅转染迷你基因的细胞中ceacam1L在两条带中所占比例为76.7%,而与ptb3种重组质粒共转染后,比例分别下降至58.3%、64.8%和54.0%。凝胶阻滞实验表明,探针GAE能与核蛋白结合,而ACE基本不能与核蛋白结合,与GAE结合的蛋白经质谱分析为PTB。结论:PTB过表达与ceacam1低表达有明显的相关性,拼接因子PTB参与ceacam1的选择性拼接。
