[Objective] This study aimed to construct DNA fingerprint for hybrid rice cultivars those have been approved by Hunan Province. [Method] The primers which produced polymorphic and bright DNA bands were selected to con...[Objective] This study aimed to construct DNA fingerprint for hybrid rice cultivars those have been approved by Hunan Province. [Method] The primers which produced polymorphic and bright DNA bands were selected to construct the DNA molecular fingerprint map for 77 major hybrid rice cultivars approved by Hunan Province. [ Result] A total of 48 SSR primers were selected. Every cultivar had its unique fingerprint map so that the obtained data could identify differ- ent hybrid rice cultivars. [ Conclusion] This study made great contributions to the perfection of hybrid rice germplasm identification.展开更多
[ Objective ] This study aimed to explore the ways of using SSR technology to construct the fingerprinting maps of the loquat. [ Method ] Fingerprinting patterns of the SSR markers for 33 loquat varieties in national ...[ Objective ] This study aimed to explore the ways of using SSR technology to construct the fingerprinting maps of the loquat. [ Method ] Fingerprinting patterns of the SSR markers for 33 loquat varieties in national loquat germplasm resource garden were constructed by specifically amplifying palymorphism from 7 pairs of primers screened from 51 pairs of primers of the relatives Mains and Pyrus. [ Result ] The results showed that the uses of 7 primers, namely, Hi01d05F, Hil2iO4F, CH02B10F, Hi03a03, Hi03e04, Hi04a05 and Hi02c07 could distinguish 33 loquat germplasms from one another completely. [ Conclusion] This study provides an essential criterion for classification and identification of the loquat resources.展开更多
基金Supported by Agricultural Science and Technology Achievements Transformation Fund of Ministry of Science and Technology (2007GB2D200225)Master Degree Paper Innovation Fund of Central South University (2010SSXt045)
文摘[Objective] This study aimed to construct DNA fingerprint for hybrid rice cultivars those have been approved by Hunan Province. [Method] The primers which produced polymorphic and bright DNA bands were selected to construct the DNA molecular fingerprint map for 77 major hybrid rice cultivars approved by Hunan Province. [ Result] A total of 48 SSR primers were selected. Every cultivar had its unique fingerprint map so that the obtained data could identify differ- ent hybrid rice cultivars. [ Conclusion] This study made great contributions to the perfection of hybrid rice germplasm identification.
基金Supported by the High-technology Program of Jiangsu Province(BG2007312)
文摘[ Objective ] This study aimed to explore the ways of using SSR technology to construct the fingerprinting maps of the loquat. [ Method ] Fingerprinting patterns of the SSR markers for 33 loquat varieties in national loquat germplasm resource garden were constructed by specifically amplifying palymorphism from 7 pairs of primers screened from 51 pairs of primers of the relatives Mains and Pyrus. [ Result ] The results showed that the uses of 7 primers, namely, Hi01d05F, Hil2iO4F, CH02B10F, Hi03a03, Hi03e04, Hi04a05 and Hi02c07 could distinguish 33 loquat germplasms from one another completely. [ Conclusion] This study provides an essential criterion for classification and identification of the loquat resources.
