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转FRO2基因番茄突变体的抗缺铁黄化特性 被引量:2
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作者 张伟玉 杨静慧 +4 位作者 刘艳军 黄俊轩 梁国鲁 乔红娥 曲佳 《西南大学学报(自然科学版)》 CAS CSCD 北大核心 2007年第3期81-85,共5页
为了增加植物的抗缺铁黄化能力,研究了转FRO2基因番茄突变体抗黄化特性.结果显示FRO2基因转化番茄后,使生长在缺铁胁迫下的植株根系铁还原酶活性大大增强(转FRO2基因番茄植株根系酶活性是对照2倍),番茄铁吸收能力和有效铁还原能... 为了增加植物的抗缺铁黄化能力,研究了转FRO2基因番茄突变体抗黄化特性.结果显示FRO2基因转化番茄后,使生长在缺铁胁迫下的植株根系铁还原酶活性大大增强(转FRO2基因番茄植株根系酶活性是对照2倍),番茄铁吸收能力和有效铁还原能力大大加强,植株中有效铁含量增加(转FRO2基因番茄植株叶片有效铁含量是时照的1.5倍),转FRO2基因番茄的植株叶片颜色显著比对照绿,黄化程度减轻,黄化指数降低(对照的黄化指数是转FRO2基因番茄的1.3倍),植株生长量显著高于对照(转基因植株株高高于对照50%).在正常条件下,转FRO2基因番茄根系的FRO2基因表达量增加,铁还原酶活性增加了1.75倍,转FRO2基因番茄植株叶片有效铁含量是对照植株的2倍,是其他耐贮藏转基因番茄的(转反义NR、ACC、CTR1基因)2~3.5倍以上. 展开更多
关键词 转基因番茄 fro2 铁胁迫 铁还原酶活性 有效铁 黄化指数
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转基因八楞海棠抗黄化鉴定研究 被引量:4
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作者 李建科 邱苗苗 +3 位作者 杨静慧 刘艳军 黄俊轩 梁国鲁 《中国果树》 北大核心 2007年第2期13-15,I0005,共4页
研究了转FRO2基因八楞海棠抗黄化性。结果显示,转FRO2基因八楞海棠植株的FRO2基因得到了过量表达;在缺铁胁迫下,转FRO2基因八楞海棠根系铁还原酶活性为对照的2.2倍;根系铁吸收能力和有效铁还原能力明显加强,叶片中有效铁含量是对照的2.1... 研究了转FRO2基因八楞海棠抗黄化性。结果显示,转FRO2基因八楞海棠植株的FRO2基因得到了过量表达;在缺铁胁迫下,转FRO2基因八楞海棠根系铁还原酶活性为对照的2.2倍;根系铁吸收能力和有效铁还原能力明显加强,叶片中有效铁含量是对照的2.1倍;转FRO2基因叶片颜色比对照绿,黄化程度减轻。在正常培养条件下,转FRO2基因八楞海棠根系的FRO2基因表达量增加,铁还原酶活性是对照的1.88倍,叶片有效铁含量是对照植株的1.7倍。转FRO2基因八楞海棠可以用于盐碱地及其缺铁土壤的生产,以解决苹果黄化问题。 展开更多
关键词 转基因八楞海棠 fro2 铁胁迫 铁还原酶活性 有效铁
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FRO_2基因表达与铁还原酶活性及有效铁吸收的关系 被引量:1
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作者 武春霞 邱苗苗 +3 位作者 杨静慧 刘艳军 黄俊轩 梁国鲁 《西南大学学报(自然科学版)》 CAS CSCD 北大核心 2007年第12期118-122,共5页
研究了转FRO2基因苹果突变体AFRO2的FRO2基因表达、铁还原酶活性和根系对有效铁的吸收.结果显示,FRO2基因在缺铁条件下大量表达;其表达量在根系中最强,其次是果实,叶片中最弱;在正常培养条件下,转基因AFRO2表达高于对照和其它非转基因品... 研究了转FRO2基因苹果突变体AFRO2的FRO2基因表达、铁还原酶活性和根系对有效铁的吸收.结果显示,FRO2基因在缺铁条件下大量表达;其表达量在根系中最强,其次是果实,叶片中最弱;在正常培养条件下,转基因AFRO2表达高于对照和其它非转基因品种;在缺铁胁迫下转基因AFRO2根系铁还原酶活性增加最多,吸收有效铁也最多.综合分析表明:FRO2基因表达量、铁还原酶活性、叶片有效铁含量3个指标有相同的变化规律,均为缺铁胁迫下最强.这说明,由于FRO2基因的转化和在八棱海棠苹果中的过量表达,使其编码的铁还原酶活性增加,根系还原和吸收的有效铁增多,致使其叶片中有效铁的积累最多. 展开更多
关键词 转基因苹果 fro2基因表达 铁胁迫 铁还原酶 有效铁
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Expression of Ferric Chelate Reductase Gene in Citrus junos and Poncirus trifoliataTissues 被引量:1
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作者 李凌 范艳华 +2 位作者 罗小英 裴炎 周泽扬 《Acta Botanica Sinica》 CSCD 2002年第7期771-774,共4页
It has been hypothesized that under iron stress high ferric chelate reductase (FCR) activity in the absorptive root of plants tolerant to iron_deficiency will be induced and result in subsequent Fe 2+ transport a... It has been hypothesized that under iron stress high ferric chelate reductase (FCR) activity in the absorptive root of plants tolerant to iron_deficiency will be induced and result in subsequent Fe 2+ transport across the plasmalemma. The activity of FCR and expression of FCR gene (FRO2) in Citrus junos Sieb. ex Tanaka tolerant to iron_deficiency and Poncirus trifoliata (L.) Raf. susceptible to iron_deficiency were determined to elucidate the physiological difference which causes the different tolerance of the two citrus rootstocks to iron stress. The activity of FCR was detectable in excised roots and was stimulated about 20_times in C. junos and only about 3_times in P. trifoliata under iron deficiency for four weeks. The FRO2 of Arabidopsis was used as a probe, the tissue print technique was used to ascertain the expression of the FCR gene in C. junos and P. trifoliata under iron stress. High_level transcripts were observed in the absorptive root, young green stem as well as new leaf of C. junos under iron stress for two weeks, and the transcripts were accumulated only slightly in P. trifoliata at the same time. The results showed that the obvious increase of FCR activity was an important reason for the tolerance of C. junos to iron_deficiency, and the regulation of FCR activity seemed to be at the transcriptional level, and the expression of FRO2 occurred in the root, stem and leaf. 展开更多
关键词 Citrus junos Poncirus trifoliata iron stress ferric chelate_reductase tissue print fro2 gene Northern hybridization
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Active-layer evolution and (CH3NH3)3Bi2l9-based solar substrate efficiency improvement of cell on TiO2-deposited ITO 被引量:1
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作者 Xinqian Zhang Gang Wu +7 位作者 Zhuowei Gu Bing Guo Wenqing Liu Shida Yang Tao Ye Chen Chen Weiwei Tu Hongzheng Chen 《Nano Research》 SCIE EI CAS CSCD 2016年第10期2921-2930,共10页
We systematically investigated the development of film morphology and crystallinity of methyl-ammonium bismuth (Ⅲ) iodide (MA3Bi2I9) through one- step spin-coating on TiO2-deposited indium tin oxide (ITO)/glass... We systematically investigated the development of film morphology and crystallinity of methyl-ammonium bismuth (Ⅲ) iodide (MA3Bi2I9) through one- step spin-coating on TiO2-deposited indium tin oxide (ITO)/glass. The precursor solution concentration and substrate structure have been demonstrated to be critically important in the active-layer evolution of the MA3Bi2I9-based solar cell. This work successfully improved the cell efficiency to 0.42% (average: 0.38%) with the mesoscopic architecture of ITO/compact-TiOdmesoscopic-TiO2 (meso-TiO2)/ MA3Bi2I9/2,2',7,7'-tetrakis(N,N-di-4-methoxyphenylamino)-9,9'spiro-bifluorene (spiro-MeOTAD)/MoO3/Ag under a precursor concentration of 0.45 M, which provided the probability of further improving the efficiency of the BiB+-based lead-free organic-inorganic hybrid solar cells. 展开更多
关键词 methyl-ammonium bismuth (Ⅲ) iodide organic-inorganic hybridsolar cell TiO2-depostied indium tinoxide (FRO) substrate morphology and crystallinity precursor solution concentration
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