In order to improve the performance of voice conversion, the fundamental frequency （F0） transformation methods are investigated, and an efficient F0 transformation algorithm is proposed. First, unlike the traditiona...In order to improve the performance of voice conversion, the fundamental frequency （F0） transformation methods are investigated, and an efficient F0 transformation algorithm is proposed. First, unlike the traditional linear transformation methods, the relationships between F0s and spectral parameters are explored. In each component of the Gaussian mixture model （GMM）, the F0s are predicted from the converted spectral parameters using the support vector regression （SVR） method. Then, in order to reduce the over- smoothing caused by the statistical average of the GMM, a mixed transformation method combining SVR with the traditional mean-variance linear （MVL） conversion is presented. Meanwhile, the adaptive median filter, prevalent in image processing, is adopted to solve the discontinuity problem caused by the frame-wise transformation. Objective and subjective experiments are carried out to evaluate the performance of the proposed method. The results demonstrate that the proposed method outperforms the traditional F0 transformation methods in terms of the similarity and the quality.展开更多
The protocol is the foundation of IoT technology, which plays an important role in the IoT device interworking and interoperability. The 5 G wireless communication system provides large-scale NB-IoT terminals access w...The protocol is the foundation of IoT technology, which plays an important role in the IoT device interworking and interoperability. The 5 G wireless communication system provides large-scale NB-IoT terminals access with F-RAN, and the vertical industry applications need support for the device management. As one of the most influential protocol in the IoT application, the oneM2M protocol not only has a complete architecture, but also has an interface with other IoT protocols. Therefore, to bridge the gap between the operator and industrial enterprises, the main contributions of this paper are as follows: Firstly, a general multi-protocol conversion method is proposed based on oneM2M platform where the protocol classification is used in different scenarios. Secondly, the F-RAN architecture of oneM2M platform is designed and implemented with NB-IoT device access. Thirdly, a multiplexing scheme to process the device information is proposed for interworking proxy entity(IPE), which improves the conversion efficiency for different protocols. Finally, the feasibility and efficiency of the scheme are verified.展开更多
Objective To calculate couple-year of protection （CYP） by conversion coefficient （F） of tubectomy and vasectomy in urban and rural regions of Iran. Methods A total of 103 450 married women aged 10-49 years in 2005...Objective To calculate couple-year of protection （CYP） by conversion coefficient （F） of tubectomy and vasectomy in urban and rural regions of Iran. Methods A total of 103 450 married women aged 10-49 years in 2005 across urban and rural regions of lran were sampled by multi stage cluster sampling. The data were collected by household survey and direct interview and analyzed by STATA8.0 software and survey analysis commands. Results Mean age of the women at the time of tubectomy estimated 31.9 years and conversion coefficient of tubectomy was 17.1 ±0.1. Mean age of women at the time of her husband vasectomy estimated 31.48 years and its conversion coefficient was 17. 5 ± 0. 1. Cluster analysis defined different regions of Iran on the basis of evaluated conversion coefficients of tubectomy and vasectomy which both of them presented seven clusters. Literacy of women and total coverage of family planning in a region had a direct relationship with this conversion coefficient （P〈0.05）. Conclusion The variations observed in the conversion coefficients and their dissimilarity among different regions of Iran may be due to a variety of fundamental factors of which literacy and culture are of important factors.展开更多
Background:The JAK2^(V617F)mutation plays a crucial part in the pathogenesis of myeloproliferative neoplasms(MPN),which includes polycythemia vera(PV),essential thrombocythemia(ET),and primary myelofibrosis(PMF)leadin...Background:The JAK2^(V617F)mutation plays a crucial part in the pathogenesis of myeloproliferative neoplasms(MPN),which includes polycythemia vera(PV),essential thrombocythemia(ET),and primary myelofibrosis(PMF)leading to aberrant proliferation and survival of hematopoietic cells.Alongside the challenges of drug resistance and side effects,identifying novel compounds that selectively target JAK2^(V617F)could provide more effective and safer therapeutic options for patients with MPNs.Materials and Methods:We employed computational approaches like high-throughput virtual screening,molecular dynamics simulations(MDS),and binding free energy calculations to identify inhibitors targeting wild and mutant JAK2 kinases.JAK2^(V617F)positive HEL,wild type JAK2 positive TF-1,and non-cancerous Vero cells were used for in vitro validations.Results:SBLJ23 emerged as a top candidate inhibitor with specificity for JAK2^(V617F).Protein-ligand interaction studies and MDS revealed stable interactions and binding of SBLJ23 over the simulation period,with Root Mean Square Deviation(RMSD)indicating consistent binding after 1t15ns.SBLJ23 displayed a half maximal inhibitory concentration(IC_(50))value of 522.4 nM against the JAK2 enzyme.The compound exhibited inhibition of cell proliferation in HEL and TF-1 cells,with half maximal cell growth inhibitory concentration(GI 50)values of 2.51 and 15.87μM,respectively.Moreover,SBLJ23 induced G 2/M cell cycle arrest in HEL cells to facilitate apoptosis in these cell lines.The compound significantly reduced the percentage of phospho JAK2 and phospho STAT3 in HEL cells.Conclusion:High binding affinity,stable interaction profile,favorable binding free energy,and in vitro validations claim SBLJ23 as a potential lead compound against JAK2^(V617F)and suggest further development and optimization towards clinical application in managing myeloproliferative neoplasms.展开更多
基金The National Natural Science Foundation of China(No. 60975017)the Natural Science Foundation of Guangdong Province (No. 10252800001000001)the Natural Science Foundation of Higher Education Institutions of Jiangsu Province (No. 10KJB510005)
文摘In order to improve the performance of voice conversion, the fundamental frequency （F0） transformation methods are investigated, and an efficient F0 transformation algorithm is proposed. First, unlike the traditional linear transformation methods, the relationships between F0s and spectral parameters are explored. In each component of the Gaussian mixture model （GMM）, the F0s are predicted from the converted spectral parameters using the support vector regression （SVR） method. Then, in order to reduce the over- smoothing caused by the statistical average of the GMM, a mixed transformation method combining SVR with the traditional mean-variance linear （MVL） conversion is presented. Meanwhile, the adaptive median filter, prevalent in image processing, is adopted to solve the discontinuity problem caused by the frame-wise transformation. Objective and subjective experiments are carried out to evaluate the performance of the proposed method. The results demonstrate that the proposed method outperforms the traditional F0 transformation methods in terms of the similarity and the quality.
基金supported by the Fundamental Research Funds for the Central Universities(2019PTB-017)
文摘The protocol is the foundation of IoT technology, which plays an important role in the IoT device interworking and interoperability. The 5 G wireless communication system provides large-scale NB-IoT terminals access with F-RAN, and the vertical industry applications need support for the device management. As one of the most influential protocol in the IoT application, the oneM2M protocol not only has a complete architecture, but also has an interface with other IoT protocols. Therefore, to bridge the gap between the operator and industrial enterprises, the main contributions of this paper are as follows: Firstly, a general multi-protocol conversion method is proposed based on oneM2M platform where the protocol classification is used in different scenarios. Secondly, the F-RAN architecture of oneM2M platform is designed and implemented with NB-IoT device access. Thirdly, a multiplexing scheme to process the device information is proposed for interworking proxy entity(IPE), which improves the conversion efficiency for different protocols. Finally, the feasibility and efficiency of the scheme are verified.
文摘Objective To calculate couple-year of protection （CYP） by conversion coefficient （F） of tubectomy and vasectomy in urban and rural regions of Iran. Methods A total of 103 450 married women aged 10-49 years in 2005 across urban and rural regions of lran were sampled by multi stage cluster sampling. The data were collected by household survey and direct interview and analyzed by STATA8.0 software and survey analysis commands. Results Mean age of the women at the time of tubectomy estimated 31.9 years and conversion coefficient of tubectomy was 17.1 ±0.1. Mean age of women at the time of her husband vasectomy estimated 31.48 years and its conversion coefficient was 17. 5 ± 0. 1. Cluster analysis defined different regions of Iran on the basis of evaluated conversion coefficients of tubectomy and vasectomy which both of them presented seven clusters. Literacy of women and total coverage of family planning in a region had a direct relationship with this conversion coefficient （P〈0.05）. Conclusion The variations observed in the conversion coefficients and their dissimilarity among different regions of Iran may be due to a variety of fundamental factors of which literacy and culture are of important factors.
文摘Background:The JAK2^(V617F)mutation plays a crucial part in the pathogenesis of myeloproliferative neoplasms(MPN),which includes polycythemia vera(PV),essential thrombocythemia(ET),and primary myelofibrosis(PMF)leading to aberrant proliferation and survival of hematopoietic cells.Alongside the challenges of drug resistance and side effects,identifying novel compounds that selectively target JAK2^(V617F)could provide more effective and safer therapeutic options for patients with MPNs.Materials and Methods:We employed computational approaches like high-throughput virtual screening,molecular dynamics simulations(MDS),and binding free energy calculations to identify inhibitors targeting wild and mutant JAK2 kinases.JAK2^(V617F)positive HEL,wild type JAK2 positive TF-1,and non-cancerous Vero cells were used for in vitro validations.Results:SBLJ23 emerged as a top candidate inhibitor with specificity for JAK2^(V617F).Protein-ligand interaction studies and MDS revealed stable interactions and binding of SBLJ23 over the simulation period,with Root Mean Square Deviation(RMSD)indicating consistent binding after 1t15ns.SBLJ23 displayed a half maximal inhibitory concentration(IC_(50))value of 522.4 nM against the JAK2 enzyme.The compound exhibited inhibition of cell proliferation in HEL and TF-1 cells,with half maximal cell growth inhibitory concentration(GI 50)values of 2.51 and 15.87μM,respectively.Moreover,SBLJ23 induced G 2/M cell cycle arrest in HEL cells to facilitate apoptosis in these cell lines.The compound significantly reduced the percentage of phospho JAK2 and phospho STAT3 in HEL cells.Conclusion:High binding affinity,stable interaction profile,favorable binding free energy,and in vitro validations claim SBLJ23 as a potential lead compound against JAK2^(V617F)and suggest further development and optimization towards clinical application in managing myeloproliferative neoplasms.
