A chemiluminescence enzyme immunoassay based on magnetic microparticles(MmPs-CLEIA)was developed to evaluate serum a-fetoprotein(AFP)in parallel with traditional colorimetric enzyme-linked immunosorbent assay(ELISA).A...A chemiluminescence enzyme immunoassay based on magnetic microparticles(MmPs-CLEIA)was developed to evaluate serum a-fetoprotein(AFP)in parallel with traditional colorimetric enzyme-linked immunosorbent assay(ELISA).A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents,less total assay time,and better linearity,recovery,precision,sensitivity and validity.AFP was detected in forty human serum samples by the proposed MPs-CLEIA and ELISA,and the results were compared with commercial electrochemiluminescence immunoassay(ECLIA)kit.The correlation coefficient between MPs-CLEIA and ELISA was obtained with R 20.6703;however,the correlation between MPs-CLEIA and ECLIA(R 20.9582)was obviously better than that between colorimetric ELISA and ECLIA(R 20.6866).展开更多
Common bunt is a major disease of wheat worldwide that reduces crop yields and grain quality.Rapid and sensitive quantitative detection methods are required to diagnose and monitor this disease in wheat management pro...Common bunt is a major disease of wheat worldwide that reduces crop yields and grain quality.Rapid and sensitive quantitative detection methods are required to diagnose and monitor this disease in wheat management programs and to ensure seed quality.In this study,an immunomagnetic beads-based enzyme-linked immunosorbent assay(IMBs-ELISA)was developed for the detection of Tilletia foetida teliospores in wheat and flour.An anti-T.foetida teliospores polyclonal antibody bound to immunomagnetic beads was used as the capture probe,and a polyclonal antibody labeled with horseradish peroxidase was used as the detector probe.The capture and detection conditions for the target spores were optimized to achieve the best determination results.Under optimal conditions,the proposed method took less than 2 h to complete.Its limit of detection was 300 teliospores per gram.The critical reaction in this IMBs-ELISA occurred on magnetic beads.This not only simplified the traditional ELISA process,but also shortened the detection time.This study has expanded the application of the IMBs-ELISA method to fungal spore detection.This method has potential applications in agriculture and seed management.展开更多
基金supported by the National Basic Research Program of China(973 Program,no.2007CB714507)National Nature Science Foundation of China(no.90813015)
文摘A chemiluminescence enzyme immunoassay based on magnetic microparticles(MmPs-CLEIA)was developed to evaluate serum a-fetoprotein(AFP)in parallel with traditional colorimetric enzyme-linked immunosorbent assay(ELISA).A systematic comparison between the MmPs-CLEIA and colorimetric ELISA concluded that the MPs-CLEIA exhibited fewer dosages of immunoreagents,less total assay time,and better linearity,recovery,precision,sensitivity and validity.AFP was detected in forty human serum samples by the proposed MPs-CLEIA and ELISA,and the results were compared with commercial electrochemiluminescence immunoassay(ECLIA)kit.The correlation coefficient between MPs-CLEIA and ELISA was obtained with R 20.6703;however,the correlation between MPs-CLEIA and ECLIA(R 20.9582)was obviously better than that between colorimetric ELISA and ECLIA(R 20.6866).
基金supported by the National Key Research&Development Program(2024YFF0618103,2023YFF0611503,2023YFF1105102)the Science and Technology Program of State Administration for Market Regulation(2024MK170)+1 种基金the Science and Technology Project of Jiangsu Market Supervision and Administration(KJ2024014,KJ2024059)the Science and Technology Program of Nanjing Market Supervision and Administration(Kj2023006).
文摘Common bunt is a major disease of wheat worldwide that reduces crop yields and grain quality.Rapid and sensitive quantitative detection methods are required to diagnose and monitor this disease in wheat management programs and to ensure seed quality.In this study,an immunomagnetic beads-based enzyme-linked immunosorbent assay(IMBs-ELISA)was developed for the detection of Tilletia foetida teliospores in wheat and flour.An anti-T.foetida teliospores polyclonal antibody bound to immunomagnetic beads was used as the capture probe,and a polyclonal antibody labeled with horseradish peroxidase was used as the detector probe.The capture and detection conditions for the target spores were optimized to achieve the best determination results.Under optimal conditions,the proposed method took less than 2 h to complete.Its limit of detection was 300 teliospores per gram.The critical reaction in this IMBs-ELISA occurred on magnetic beads.This not only simplified the traditional ELISA process,but also shortened the detection time.This study has expanded the application of the IMBs-ELISA method to fungal spore detection.This method has potential applications in agriculture and seed management.
基金Project supported by National Natural Science Foundation of China (No. 20675048)National High-Tech Research and Development Program of China (863 Program, No. 07AA10Z435, No. 2007AA06A407)National Basic Research Program of China (973 Program, No. 2007CB936602)
