To evaluate the effect of GnRHa induced superovulation protocol on endometrial morphology and function. Material & Methods Forty ICR mice were randomly allocated into 4 groups, among them, 2 experimental gro...To evaluate the effect of GnRHa induced superovulation protocol on endometrial morphology and function. Material & Methods Forty ICR mice were randomly allocated into 4 groups, among them, 2 experimental groups were injected with GnRHa+HMG+hCG, another 2 groups were given saline of same volume as control group. The uterine tissues were investigated at 24 h and 48 h after administration (experimental group) or ovulation (control group).The endometrial thickness, the size of gland and glandular lumen, the total area of glandular cells, the average height of glandular epithelium were measured from routine histological slides using computerized image analysis. The SP immunohistochemistry techniques with monoclonal antibodies were employed to semi quantitatively analize the estrogen receptor (ER) and progesterone receptor (PR) in glandular cells. Results The endometrial thickness was not significantly different between experimental groups and control groups at 24 h and 48 h (P>0.05).The average area, perimeter, maximal diameter of single gland and glandular lumen, the total area, average height of glandular epithelium in experimental groups were significantly smaller than those of in control groups at equivalent time stages (all P<0.01). The asynchronous development of gland epithelium and stroma cells, namely, pesudostratified glandular epithelium and predecidual changes of stroma cells were seen at same time in experimental groups. The positive percentage (%) and expression intense of ER and PR in glandular epithelium cells were significantly lower in experimental groups than in control groups (P<0.05). Conclusion The protocol with GnRHa had a negative effect on endometrial histological structure and down regulated the express of ER and PR, suggesting that this protocol effect on the endometrial morphology and function and could not facilitate the formation of a physiologic endometrium completely, which may be one of the causes of low pregnancy rates.展开更多
Introduction-Breast cancer is the most common cancer and leading cause of death in women. In India, its incidence is rapidly rising over last few decades. Present study is aimed to study the pattern of expression of h...Introduction-Breast cancer is the most common cancer and leading cause of death in women. In India, its incidence is rapidly rising over last few decades. Present study is aimed to study the pattern of expression of hormonal receptors and Her-2/neu in invasive breast carcinoma and to correlate estrogen receptor (ER), progesterone receptor (PR) and Her-2/neu expressions with various clinicopathological parameters. Material and methods: The present study was carried out in Department of Pathology, S.C.B. Medical College, Cuttack in the year 2013 taking consecutive 100 cases. Routine H&E staining for histological diagnosis and IHC analysis for ER, PR and Her 2/neu was carried out in all 100 cases of breast malignancies. Results: 99% of cases are invasive breast carcinoma, not otherwise specify (IDC-NOS). The age ranges from 23 years to 72 years. Majority of tumors are of grade 2 (70%) followed by grade 3 (30%). ER PR and Her-2/neu expression are seen in 45%, 35% and 30% respectively. Triple negative cases comprise 35%. Higher number of grade 2 tumor shows ER, PR positivity as compared to grade 3 tumors. Her-2/neu expression does not show any significant correlation with age or lymph node status of the patient. Conclusion: ER and PR expression in breast cancers in the current study are found to be comparable to the findings of other authors, but the frequency of HER-2/neu expression is slightly higher. Significant correlation is observed between hormonal receptor status and the grade of the tumor. Inverse relationship is found between Her-2/neu expression and ER, PR receptor status. Her-2/neu expression is increased with size and high grade of tumor.展开更多
Objective: To study the relationship between theexpression of PS, protein and Estrogen (ER) andProgesterone Receptor (PR) status and their prognoticvalue in breast canceL Methods: Using theimmunohistochemical method, ...Objective: To study the relationship between theexpression of PS, protein and Estrogen (ER) andProgesterone Receptor (PR) status and their prognoticvalue in breast canceL Methods: Using theimmunohistochemical method, PS2 protein expressionswere detected in 105 cases with breast cancer. Results:The positive rate of PS2 protein was 50.48% (53/105) in105 cases. The positive rate of PS, in the patients whosurvived five years or more was 56.96% (45/79), whichwas higher than that of those who lived less than fiveyears (30.77%, 8/26). In the ER, PR (+) patients, thepositive rate of PS, was higher (76.74%, 33/34), thanthat of those with ER, PR (-) (22.5%, 9/40). Conclusions:Our results suggest that the expression of PS, proteinwas positively correlated with the 5-year-survival andthat of ER and PR in breast cancer. It is considered thatPS, may be as a prognostic predictor, and detection ofPS, protein expression was useful for a guidingtreatment of breast cancer.展开更多
From February to September in 1994, a total of 58 patients with nasopharyngeal carcinoma(NPC) were testified by Pathology. It included 56 poorly differentiated squamous cancer, 1 poorly differentiated adenocarcinoma, ...From February to September in 1994, a total of 58 patients with nasopharyngeal carcinoma(NPC) were testified by Pathology. It included 56 poorly differentiated squamous cancer, 1 poorly differentiated adenocarcinoma, 1 B-cell lymphoma. Clinical staging: II stage 6 cases, III stage 20 cases, Ⅳ stage 24 cases,recurrence 8 cases. The results showed that ER and PR positive rate were 26/58(44.8%) and 28/58(48.3%). The rate has not relation to the age and sex. There was a contrary rela-tion between ER, PR Positive rate and clinical staging and VCA-IgA. When ER, PR were negative and VCA-IgA was 1:40 in IV stage patients, its accorded rate was 9/12(75%); when only VCA-IgA was 1:40, accorded rate was 13/(39.4%). Total CR was 38/49 (77.6%). When both ER and PR were Positive, CR was 79%; when both ER and PR were negative and VCAIgA was 1:40, CR was 58.3%. We regard NPC is belong to high expression tumor of estrogen and progesterone,and positive expression can reflect tumor developing tendency and relate to recent prognosis. The paper suggests that it is possible to treat NPC with the together therapy. Examining ER, PR and VCA-IgA together can improve the analysis of the prognosis of NPC.展开更多
The rare earth elements contents (La, Ce, Pr, Nd, Sm) in 150 Pu er tea samples were analyzed by using inductively coupled plasma atomic emission spectroscopy (ICP-AES), to evaluate the safety of Pu er tea. Among the i...The rare earth elements contents (La, Ce, Pr, Nd, Sm) in 150 Pu er tea samples were analyzed by using inductively coupled plasma atomic emission spectroscopy (ICP-AES), to evaluate the safety of Pu er tea. Among the investigated rare earth elements (REEs), Ce was the highest (35% of the total REEs content) whose concentrations were found to be in the range of 0.11–1.39 mg/kg, whereas that of Sm in the range 0.01–0.21 mg/kg, showing minimum levels (5.2%). La was the second highest element (28%) found in Pu e...展开更多
The 3μm laser emission of Er 3+ ( 4 I 11/2 → 4 I 13/2 )in the YAG crystal is,in principle,selfsaturation transition,the lifetime of the upper laser level( 4 I 11/2 )being smaller than that of the olwer one( 4 I 13/2...The 3μm laser emission of Er 3+ ( 4 I 11/2 → 4 I 13/2 )in the YAG crystal is,in principle,selfsaturation transition,the lifetime of the upper laser level( 4 I 11/2 )being smaller than that of the olwer one( 4 I 13/2 ).The effect of the selfsaturation limit the laser output. In present work,for the first time,we report on the investigation of the utility of Pr 3+ as a sensitizer of Er 3+ ion,using Pr 3+ 4 f 2-4 f5d transition.The radiation emission from 4f5d configuration of Pr 3+ ion have been observed in the YAG∶Pr 3+ crystal.This shows that under suitable conditions this radiant process can compete successfully by non radiative decay to the 4 f configuration.Using Er 3+ Pr 3 codoped YAG it is possible to predict the position of the lowest 4 f 2-4 f5d absorption bands of Pr 3+ ion in the ultraviolet range and to present the occurrence of the energy transfer from Pr 3+ to Er 3+ ion.By xenon flash lamp pumped 2.93μm laser action in the YAG∶(Er 3+ ,Pr 3+ ) crystals was demonstrated at room temperature.展开更多
文摘To evaluate the effect of GnRHa induced superovulation protocol on endometrial morphology and function. Material & Methods Forty ICR mice were randomly allocated into 4 groups, among them, 2 experimental groups were injected with GnRHa+HMG+hCG, another 2 groups were given saline of same volume as control group. The uterine tissues were investigated at 24 h and 48 h after administration (experimental group) or ovulation (control group).The endometrial thickness, the size of gland and glandular lumen, the total area of glandular cells, the average height of glandular epithelium were measured from routine histological slides using computerized image analysis. The SP immunohistochemistry techniques with monoclonal antibodies were employed to semi quantitatively analize the estrogen receptor (ER) and progesterone receptor (PR) in glandular cells. Results The endometrial thickness was not significantly different between experimental groups and control groups at 24 h and 48 h (P>0.05).The average area, perimeter, maximal diameter of single gland and glandular lumen, the total area, average height of glandular epithelium in experimental groups were significantly smaller than those of in control groups at equivalent time stages (all P<0.01). The asynchronous development of gland epithelium and stroma cells, namely, pesudostratified glandular epithelium and predecidual changes of stroma cells were seen at same time in experimental groups. The positive percentage (%) and expression intense of ER and PR in glandular epithelium cells were significantly lower in experimental groups than in control groups (P<0.05). Conclusion The protocol with GnRHa had a negative effect on endometrial histological structure and down regulated the express of ER and PR, suggesting that this protocol effect on the endometrial morphology and function and could not facilitate the formation of a physiologic endometrium completely, which may be one of the causes of low pregnancy rates.
文摘Introduction-Breast cancer is the most common cancer and leading cause of death in women. In India, its incidence is rapidly rising over last few decades. Present study is aimed to study the pattern of expression of hormonal receptors and Her-2/neu in invasive breast carcinoma and to correlate estrogen receptor (ER), progesterone receptor (PR) and Her-2/neu expressions with various clinicopathological parameters. Material and methods: The present study was carried out in Department of Pathology, S.C.B. Medical College, Cuttack in the year 2013 taking consecutive 100 cases. Routine H&E staining for histological diagnosis and IHC analysis for ER, PR and Her 2/neu was carried out in all 100 cases of breast malignancies. Results: 99% of cases are invasive breast carcinoma, not otherwise specify (IDC-NOS). The age ranges from 23 years to 72 years. Majority of tumors are of grade 2 (70%) followed by grade 3 (30%). ER PR and Her-2/neu expression are seen in 45%, 35% and 30% respectively. Triple negative cases comprise 35%. Higher number of grade 2 tumor shows ER, PR positivity as compared to grade 3 tumors. Her-2/neu expression does not show any significant correlation with age or lymph node status of the patient. Conclusion: ER and PR expression in breast cancers in the current study are found to be comparable to the findings of other authors, but the frequency of HER-2/neu expression is slightly higher. Significant correlation is observed between hormonal receptor status and the grade of the tumor. Inverse relationship is found between Her-2/neu expression and ER, PR receptor status. Her-2/neu expression is increased with size and high grade of tumor.
文摘Objective: To study the relationship between theexpression of PS, protein and Estrogen (ER) andProgesterone Receptor (PR) status and their prognoticvalue in breast canceL Methods: Using theimmunohistochemical method, PS2 protein expressionswere detected in 105 cases with breast cancer. Results:The positive rate of PS2 protein was 50.48% (53/105) in105 cases. The positive rate of PS, in the patients whosurvived five years or more was 56.96% (45/79), whichwas higher than that of those who lived less than fiveyears (30.77%, 8/26). In the ER, PR (+) patients, thepositive rate of PS, was higher (76.74%, 33/34), thanthat of those with ER, PR (-) (22.5%, 9/40). Conclusions:Our results suggest that the expression of PS, proteinwas positively correlated with the 5-year-survival andthat of ER and PR in breast cancer. It is considered thatPS, may be as a prognostic predictor, and detection ofPS, protein expression was useful for a guidingtreatment of breast cancer.
文摘From February to September in 1994, a total of 58 patients with nasopharyngeal carcinoma(NPC) were testified by Pathology. It included 56 poorly differentiated squamous cancer, 1 poorly differentiated adenocarcinoma, 1 B-cell lymphoma. Clinical staging: II stage 6 cases, III stage 20 cases, Ⅳ stage 24 cases,recurrence 8 cases. The results showed that ER and PR positive rate were 26/58(44.8%) and 28/58(48.3%). The rate has not relation to the age and sex. There was a contrary rela-tion between ER, PR Positive rate and clinical staging and VCA-IgA. When ER, PR were negative and VCA-IgA was 1:40 in IV stage patients, its accorded rate was 9/12(75%); when only VCA-IgA was 1:40, accorded rate was 13/(39.4%). Total CR was 38/49 (77.6%). When both ER and PR were Positive, CR was 79%; when both ER and PR were negative and VCAIgA was 1:40, CR was 58.3%. We regard NPC is belong to high expression tumor of estrogen and progesterone,and positive expression can reflect tumor developing tendency and relate to recent prognosis. The paper suggests that it is possible to treat NPC with the together therapy. Examining ER, PR and VCA-IgA together can improve the analysis of the prognosis of NPC.
基金Project supported by the Foundation of National Key Technology R & D Program (2007BAD58B05-3)
文摘The rare earth elements contents (La, Ce, Pr, Nd, Sm) in 150 Pu er tea samples were analyzed by using inductively coupled plasma atomic emission spectroscopy (ICP-AES), to evaluate the safety of Pu er tea. Among the investigated rare earth elements (REEs), Ce was the highest (35% of the total REEs content) whose concentrations were found to be in the range of 0.11–1.39 mg/kg, whereas that of Sm in the range 0.01–0.21 mg/kg, showing minimum levels (5.2%). La was the second highest element (28%) found in Pu e...
文摘The 3μm laser emission of Er 3+ ( 4 I 11/2 → 4 I 13/2 )in the YAG crystal is,in principle,selfsaturation transition,the lifetime of the upper laser level( 4 I 11/2 )being smaller than that of the olwer one( 4 I 13/2 ).The effect of the selfsaturation limit the laser output. In present work,for the first time,we report on the investigation of the utility of Pr 3+ as a sensitizer of Er 3+ ion,using Pr 3+ 4 f 2-4 f5d transition.The radiation emission from 4f5d configuration of Pr 3+ ion have been observed in the YAG∶Pr 3+ crystal.This shows that under suitable conditions this radiant process can compete successfully by non radiative decay to the 4 f configuration.Using Er 3+ Pr 3 codoped YAG it is possible to predict the position of the lowest 4 f 2-4 f5d absorption bands of Pr 3+ ion in the ultraviolet range and to present the occurrence of the energy transfer from Pr 3+ to Er 3+ ion.By xenon flash lamp pumped 2.93μm laser action in the YAG∶(Er 3+ ,Pr 3+ ) crystals was demonstrated at room temperature.
