目的探讨蛋白质EPB41(erythrocyte membrane protein band 4.1)对食管癌细胞恶性表型的影响,并基于Wnt/β-catenin信号通路介导的脂肪酸氧化(fatty acid oxidation,FAO)探讨可能的潜在机制。方法将对数生长期的KYSE30细胞及KYSE150细胞...目的探讨蛋白质EPB41(erythrocyte membrane protein band 4.1)对食管癌细胞恶性表型的影响,并基于Wnt/β-catenin信号通路介导的脂肪酸氧化(fatty acid oxidation,FAO)探讨可能的潜在机制。方法将对数生长期的KYSE30细胞及KYSE150细胞各分为5组,检测mRNA和蛋白质表达、细胞增殖和迁移率、ATP水平、NADPH/NADP^(+)比率、活性氧(ROS)和GSH水平。结果在KYSE30细胞中,转染EPB41 siRNA组细胞克隆数、细胞迁移率、ATP水平、NADPH/NADP^(+)比率、ROS水平、细胞中Wnt3a、β-catenin和核β-catenin蛋白质表达均明显增加(P<0.05),且脂肪酸氧化抑制剂和Wnt通路抑制剂可逆转EPB41的作用;在KYSE150细胞中,转染EPB41过表达载体oe-EPB41组细胞克隆数、细胞迁移率、ATP水平、NADPH/NADP^(+)比率、ROS水平、细胞中Wnt3a、β-catenin和核β-catenin蛋白质表达均明显降低(P<0.05),且脂肪酸氧化诱导剂和Wnt通路激活剂可逆转EPB41的作用。结论EPB41在食管癌细胞中的表达下调,EPB41过表达可通过抑制FAO途径来抑制食管癌细胞的增殖和迁移,这可能是通过调控Wnt/β-catenin信号通路转导来实现。展开更多
In situ recycling is one of the most effective methods to dispose of earth pressure balance(EPB)shield waste muck with residual foaming agents with high moisture content.In this context,response surface methodology(RS...In situ recycling is one of the most effective methods to dispose of earth pressure balance(EPB)shield waste muck with residual foaming agents with high moisture content.In this context,response surface methodology(RSM)was employed to quantify the effects of independent variables,including flocculant dosage,defoamer dosage,and muck drying mass(MDM)and their interactions on defoaming-flocculation-dewatering indices.The polymeric aluminum chloride(PACL)and hydroxy silicone oil-glycerol polypropylene ether(H-G)were selected as the flocculant and defoamer.The contents of surfactants and foam stabilizers in residual foaming agents were determined using the proposed empirical equation.The defoaming ratio,antifoaming ratio,turbidity,moisture content,filtration loss ratio,and fall cone penetration depth were considered as dependent variables.The accuracy of developed RSM models was verified by the analysis results of variance,residuals,and paired t-test.Combined with the desirability approach,an optimal mixing ratio of 0.078 wt%PACL,0.016 wt%H-G,and 27.882 wt%MDM was recommended,leading to a defoaming ratio of 98.34 vol%for residual foams and a moisture content of 56.72 wt%for pressure-filtration cakes.Our findings were demonstrated to be able to provide useful guidance for prediction and optimization of the in situ recycling indicators of EPB shield waste muck in metro tunnel construction sites.展开更多
文摘目的探讨蛋白质EPB41(erythrocyte membrane protein band 4.1)对食管癌细胞恶性表型的影响,并基于Wnt/β-catenin信号通路介导的脂肪酸氧化(fatty acid oxidation,FAO)探讨可能的潜在机制。方法将对数生长期的KYSE30细胞及KYSE150细胞各分为5组,检测mRNA和蛋白质表达、细胞增殖和迁移率、ATP水平、NADPH/NADP^(+)比率、活性氧(ROS)和GSH水平。结果在KYSE30细胞中,转染EPB41 siRNA组细胞克隆数、细胞迁移率、ATP水平、NADPH/NADP^(+)比率、ROS水平、细胞中Wnt3a、β-catenin和核β-catenin蛋白质表达均明显增加(P<0.05),且脂肪酸氧化抑制剂和Wnt通路抑制剂可逆转EPB41的作用;在KYSE150细胞中,转染EPB41过表达载体oe-EPB41组细胞克隆数、细胞迁移率、ATP水平、NADPH/NADP^(+)比率、ROS水平、细胞中Wnt3a、β-catenin和核β-catenin蛋白质表达均明显降低(P<0.05),且脂肪酸氧化诱导剂和Wnt通路激活剂可逆转EPB41的作用。结论EPB41在食管癌细胞中的表达下调,EPB41过表达可通过抑制FAO途径来抑制食管癌细胞的增殖和迁移,这可能是通过调控Wnt/β-catenin信号通路转导来实现。
基金supported by the National Youth Top-notch Talent Support Program of China(Grant No.00389335)the National Natural Science Foundation of China(Grant No.52378392)the“Foal Eagle Program”Youth Top-notch Talent Project of Fujian Province(Grant No.00387088).
文摘In situ recycling is one of the most effective methods to dispose of earth pressure balance(EPB)shield waste muck with residual foaming agents with high moisture content.In this context,response surface methodology(RSM)was employed to quantify the effects of independent variables,including flocculant dosage,defoamer dosage,and muck drying mass(MDM)and their interactions on defoaming-flocculation-dewatering indices.The polymeric aluminum chloride(PACL)and hydroxy silicone oil-glycerol polypropylene ether(H-G)were selected as the flocculant and defoamer.The contents of surfactants and foam stabilizers in residual foaming agents were determined using the proposed empirical equation.The defoaming ratio,antifoaming ratio,turbidity,moisture content,filtration loss ratio,and fall cone penetration depth were considered as dependent variables.The accuracy of developed RSM models was verified by the analysis results of variance,residuals,and paired t-test.Combined with the desirability approach,an optimal mixing ratio of 0.078 wt%PACL,0.016 wt%H-G,and 27.882 wt%MDM was recommended,leading to a defoaming ratio of 98.34 vol%for residual foams and a moisture content of 56.72 wt%for pressure-filtration cakes.Our findings were demonstrated to be able to provide useful guidance for prediction and optimization of the in situ recycling indicators of EPB shield waste muck in metro tunnel construction sites.
