Objective:To identify the serological epidemiology of Cryptosporidium infections and to follow up on the changes in the infection profile in Southern Egypt in order to establish a suitable scheme for control and preve...Objective:To identify the serological epidemiology of Cryptosporidium infections and to follow up on the changes in the infection profile in Southern Egypt in order to establish a suitable scheme for control and prevention of cryptosporidiosis.Methods:A total of 1912(960 from human and 952 from animals)stool specimens and sera were screened for Cryptosporidium species using modified Ziehl Neelsen technique and a newly-developed enzyme-linked immunosorbent assay(ELISA).Environmental risk factors and socioeconomic data were surveyed by questionnaire between September 2016 and December 2017.Results:Totally,20.83%of the human subjects were positive for Cryptosporidium infection tested by ELISA.The seropositivity was positively correlated with age.The prevalence of Cryptosporidium infections in females was significantly higher than in males(P<0.05).The sensitivity and specificity of ELISA for Cryptosporidium were 99.06%and 88.88%,respectively.Furthermore,a high prevalence of Cryptosporidium in domestic animals(42.20%).Conclusions:The study observed that Cryptosporidium infections are common in the study area,with water sanitation,socioeconomic level;eating habits and hygienic status are considered the main risk factors for cryptosporidiosis.Therefore,environmental sanitation and health education will be useful in reducing the prevalence of infection.展开更多
A double antibodies additivity ELISA test was employed to identify the epltopes which can be recognized by monoclonal antibodies (McAbs) against IgM from B chronic lymphocyte leukemia (B-CLL). The computer grouping pr...A double antibodies additivity ELISA test was employed to identify the epltopes which can be recognized by monoclonal antibodies (McAbs) against IgM from B chronic lymphocyte leukemia (B-CLL). The computer grouping programme analysis showed that 4 and- isotypic MaAbs could be divided into two groups and 10 anti- idiotype McAbs could be divided into four groups. The result was consistent with that of the indirect sandwich ELISA and inhibition ELISA test. It suggested that there were at least 6 distinct IgM epitopes which can react specifically with 14 McAbs. Our study indicated that the combination of the additivity ELISA test and the computer grouping programme analysis is of help in studying the relationship of the structure and function of antigen.展开更多
Objective： The aim of this study was to investigate the clinical value and relevance on the serum β2-microglobulin （β2-MG） of patients with thyroid cancer. Methods： One thousand and two normal cases, 95 thyroid ...Objective： The aim of this study was to investigate the clinical value and relevance on the serum β2-microglobulin （β2-MG） of patients with thyroid cancer. Methods： One thousand and two normal cases, 95 thyroid cancer patients and 243 nodular goiter patients were selected to measure serum β2-MG levels using double-antibody sandwich enzyme-linked immunosorbent assay （ELISA）. Results： The positive rate of 7.78% in normal population （78/1002） and 31.57% in thyroid cancer patients （30/95）. There were significant differences between the normal population and thyroid cancer patients （X2 = 55.352; P = 0.000）. The positive rate of 7.81% in nodular goiter patients （19/243） and there were no significant differences between the normal population and nodular goiter patients （X2 = 0.0004; P = 0.986）, but significant differences between nodular goiter patients and thyroid cancer patients （x2 = 31.106; P = 0.000）. Meanwhile, the significant difference of the positive rate existed in between the various pathological types of thyroid cancer （X2 = 10.015; P = 0.007）, anaplastic thyroid cancer patients with the highest positive rate and The significant difference was found between the positive lymph node metastasis groups and negative lymph node metastasis groups （x2 = 4.441; P = 0.035）, the presence of distant metastasis group and absence of distant metastasis group （X2 = 9.795; P = 0.002）. Conclusion： Serum β2-MG levels and prognosis of thyroid cancer patients was negatively correlated. It showed important clinical value to detect the level of β2-MG in the early diagnosis, prognosis and the clinical observation for thyroid cancer patients.展开更多
Objective: to explore the analysis and thinking of the results of two methods for unpaid blood donors in a certain area. Methods: 2000 blood samples from unpaid blood donors in a certain area were selected as the rese...Objective: to explore the analysis and thinking of the results of two methods for unpaid blood donors in a certain area. Methods: 2000 blood samples from unpaid blood donors in a certain area were selected as the research object. The detection method was two times of different manufacturers ELISA reagents (Xinchuang/Wantai/Kehua). Six people were tested (ID-NAT) for those who were negative for both times. If positive, they were split again, subject to the (Roche or Kehua reagents) test (six people). Results: the positive rate of HIV 1/HCV/HBV NAT initial screening was 0.5%. Six samples were positive by ELISA at the same time, and five samples were positive by identification, with the positive rate of identification being 83.33%. The positive blood samples for twice, once positive and once negative were classified as three retest positive samples, and the negative samples for twice were classified as one retest negative sample. The negative initial screening positive S/CO value was 13.75 ± 2.40, and the negative S/CO value was 8.06 ± 6.05;The minimum value of positive S/CO in the initial screening of positive retest is 8.07, the minimum value of negative S/CO is 10.12, the minimum value of positive S/CO in the initial screening of negative retest is 9.13, and the minimum value of negative S/CO is 1.02. Conclusion: the results of ELISA and single ID-NAT test for unpaid blood donors are significant. The two methods can improve the accuracy of blood sample test for unpaid blood donors, and can effectively ensure the safety of clinical blood transfusion treatment.展开更多
文摘Objective:To identify the serological epidemiology of Cryptosporidium infections and to follow up on the changes in the infection profile in Southern Egypt in order to establish a suitable scheme for control and prevention of cryptosporidiosis.Methods:A total of 1912(960 from human and 952 from animals)stool specimens and sera were screened for Cryptosporidium species using modified Ziehl Neelsen technique and a newly-developed enzyme-linked immunosorbent assay(ELISA).Environmental risk factors and socioeconomic data were surveyed by questionnaire between September 2016 and December 2017.Results:Totally,20.83%of the human subjects were positive for Cryptosporidium infection tested by ELISA.The seropositivity was positively correlated with age.The prevalence of Cryptosporidium infections in females was significantly higher than in males(P<0.05).The sensitivity and specificity of ELISA for Cryptosporidium were 99.06%and 88.88%,respectively.Furthermore,a high prevalence of Cryptosporidium in domestic animals(42.20%).Conclusions:The study observed that Cryptosporidium infections are common in the study area,with water sanitation,socioeconomic level;eating habits and hygienic status are considered the main risk factors for cryptosporidiosis.Therefore,environmental sanitation and health education will be useful in reducing the prevalence of infection.
文摘A double antibodies additivity ELISA test was employed to identify the epltopes which can be recognized by monoclonal antibodies (McAbs) against IgM from B chronic lymphocyte leukemia (B-CLL). The computer grouping programme analysis showed that 4 and- isotypic MaAbs could be divided into two groups and 10 anti- idiotype McAbs could be divided into four groups. The result was consistent with that of the indirect sandwich ELISA and inhibition ELISA test. It suggested that there were at least 6 distinct IgM epitopes which can react specifically with 14 McAbs. Our study indicated that the combination of the additivity ELISA test and the computer grouping programme analysis is of help in studying the relationship of the structure and function of antigen.
文摘Objective： The aim of this study was to investigate the clinical value and relevance on the serum β2-microglobulin （β2-MG） of patients with thyroid cancer. Methods： One thousand and two normal cases, 95 thyroid cancer patients and 243 nodular goiter patients were selected to measure serum β2-MG levels using double-antibody sandwich enzyme-linked immunosorbent assay （ELISA）. Results： The positive rate of 7.78% in normal population （78/1002） and 31.57% in thyroid cancer patients （30/95）. There were significant differences between the normal population and thyroid cancer patients （X2 = 55.352; P = 0.000）. The positive rate of 7.81% in nodular goiter patients （19/243） and there were no significant differences between the normal population and nodular goiter patients （X2 = 0.0004; P = 0.986）, but significant differences between nodular goiter patients and thyroid cancer patients （x2 = 31.106; P = 0.000）. Meanwhile, the significant difference of the positive rate existed in between the various pathological types of thyroid cancer （X2 = 10.015; P = 0.007）, anaplastic thyroid cancer patients with the highest positive rate and The significant difference was found between the positive lymph node metastasis groups and negative lymph node metastasis groups （x2 = 4.441; P = 0.035）, the presence of distant metastasis group and absence of distant metastasis group （X2 = 9.795; P = 0.002）. Conclusion： Serum β2-MG levels and prognosis of thyroid cancer patients was negatively correlated. It showed important clinical value to detect the level of β2-MG in the early diagnosis, prognosis and the clinical observation for thyroid cancer patients.
文摘Objective: to explore the analysis and thinking of the results of two methods for unpaid blood donors in a certain area. Methods: 2000 blood samples from unpaid blood donors in a certain area were selected as the research object. The detection method was two times of different manufacturers ELISA reagents (Xinchuang/Wantai/Kehua). Six people were tested (ID-NAT) for those who were negative for both times. If positive, they were split again, subject to the (Roche or Kehua reagents) test (six people). Results: the positive rate of HIV 1/HCV/HBV NAT initial screening was 0.5%. Six samples were positive by ELISA at the same time, and five samples were positive by identification, with the positive rate of identification being 83.33%. The positive blood samples for twice, once positive and once negative were classified as three retest positive samples, and the negative samples for twice were classified as one retest negative sample. The negative initial screening positive S/CO value was 13.75 ± 2.40, and the negative S/CO value was 8.06 ± 6.05;The minimum value of positive S/CO in the initial screening of positive retest is 8.07, the minimum value of negative S/CO is 10.12, the minimum value of positive S/CO in the initial screening of negative retest is 9.13, and the minimum value of negative S/CO is 1.02. Conclusion: the results of ELISA and single ID-NAT test for unpaid blood donors are significant. The two methods can improve the accuracy of blood sample test for unpaid blood donors, and can effectively ensure the safety of clinical blood transfusion treatment.
