In order to reduce the harm of diseases in red-fleshed kiwifruit in Qiandongnan Prefecture and ensure product quality, a new disease in the producing areas were investigated, and pathogen identification were carried o...In order to reduce the harm of diseases in red-fleshed kiwifruit in Qiandongnan Prefecture and ensure product quality, a new disease in the producing areas were investigated, and pathogen identification were carried out. The pathogen was isolated from diseased leaves by a tissue separation method. DNA sequencing was performed by the sequence analysis of ribosomal rDNA-ITS(Internal transcribed spacer) region, and a molecular evolutionary tree was constructed by the MEGA 4.0 software. The pathogenic fungus was classified and identified by combining morphological observation finally. The results showed that the dry blight disease in leaf tip was a new disease, which was caused by Epicoccum sorghinum, and it had a serious damage to red-fleshed kiwifruit.展开更多
基金Supported by Province,Prefecture and School Science and Technology Cooperation Agreement Project of Science and Technology Department of Guizhou Province (QKH H Z[2017]7178)The Science and Technologyof Guizhou Province (2017-1166)。
文摘In order to reduce the harm of diseases in red-fleshed kiwifruit in Qiandongnan Prefecture and ensure product quality, a new disease in the producing areas were investigated, and pathogen identification were carried out. The pathogen was isolated from diseased leaves by a tissue separation method. DNA sequencing was performed by the sequence analysis of ribosomal rDNA-ITS(Internal transcribed spacer) region, and a molecular evolutionary tree was constructed by the MEGA 4.0 software. The pathogenic fungus was classified and identified by combining morphological observation finally. The results showed that the dry blight disease in leaf tip was a new disease, which was caused by Epicoccum sorghinum, and it had a serious damage to red-fleshed kiwifruit.
