[Objective]The aim was to find out the optimal methods for extracting DNA from dried butterfly specimen.[Method]A total of five methods including SDS method,SDS-mercaptoethanol-phenol method,CTAB method,saturated NaCl...[Objective]The aim was to find out the optimal methods for extracting DNA from dried butterfly specimen.[Method]A total of five methods including SDS method,SDS-mercaptoethanol-phenol method,CTAB method,saturated NaCl method,SDS-mercaptoethanol-chloroform methods were employed to extract DNA from dried butterfly specimen.[Result]SDS-mercaptoethanol-phenol method,saturated NaCl method and SDS-mercaptoethanol-chloroform method were suitable for genomic DNA extraction from dried butterfly samples,and the DNA extracted can be successfully applied to the PCR amplification of butterfly mitochondrial COI,EF-1α and CytB genes.[Conclusion]SDS-mercaptoethanol-phenol method,saturated NaCl method and SDS-mercaptoethanol-chloroform method can be used to study the extraction of DNA from dried butterfly samples,which laid foundation for study on molecular phylogenetics of butterfly.展开更多
基金Supported by Scientific Research Foundation for Doctors in Qinghai Normal University~~
文摘[Objective]The aim was to find out the optimal methods for extracting DNA from dried butterfly specimen.[Method]A total of five methods including SDS method,SDS-mercaptoethanol-phenol method,CTAB method,saturated NaCl method,SDS-mercaptoethanol-chloroform methods were employed to extract DNA from dried butterfly specimen.[Result]SDS-mercaptoethanol-phenol method,saturated NaCl method and SDS-mercaptoethanol-chloroform method were suitable for genomic DNA extraction from dried butterfly samples,and the DNA extracted can be successfully applied to the PCR amplification of butterfly mitochondrial COI,EF-1α and CytB genes.[Conclusion]SDS-mercaptoethanol-phenol method,saturated NaCl method and SDS-mercaptoethanol-chloroform method can be used to study the extraction of DNA from dried butterfly samples,which laid foundation for study on molecular phylogenetics of butterfly.
