The mechanistic target of rapamycin(m TOR) is a serine/threonine kinase that plays a pivotal role in cellular growth, proliferation, survival, and metabolism. In the central nervous system(CNS), the mTOR pathway regul...The mechanistic target of rapamycin(m TOR) is a serine/threonine kinase that plays a pivotal role in cellular growth, proliferation, survival, and metabolism. In the central nervous system(CNS), the mTOR pathway regulates diverse aspects of neural development and function. Genetic mutations within the m TOR pathway lead to severe neurodevelopmental disorders, collectively known as “mTORopathies”(Crino, 2020). Dysfunctions of m TOR, including both its hyperactivation and hypoactivation, have also been implicated in a wide spectrum of other neurodevelopmental and neurodegenerative conditions, highlighting its importance in CNS health.展开更多
Gold nanoclusters(AuNCs)are ultrasmall(<2 nm)aggregates of gold atoms that exhibit discrete electronic states,size-dependent photoluminescence,and exceptional biocompatibility,making them ideal candidates for thera...Gold nanoclusters(AuNCs)are ultrasmall(<2 nm)aggregates of gold atoms that exhibit discrete electronic states,size-dependent photoluminescence,and exceptional biocompatibility,making them ideal candidates for theranostic applications.Their tunable surface chemistry enables targeted delivery,while strong near-infrared emission and environmental responsiveness allow for sensitive detection and deep-tissue imaging.Recent advances have revealed that controlled assembly of AuNCs into higher-order architectures-guided by biological scaffolds such as nucleic acids,peptides,and proteins-can markedly enhance their optical and electronic properties through aggregation-induced emission(AiE)and stabilization of surface ligands.This review summarizes recent progress in the design and biomedical applications of AuNC assemblies generated using biomolecules as structure-directing scaffolds.Covalent and noncovalent interactions with biomolecules enable the formation of well-defined one-,two-,and three-dimensional structures with tunable morphologies and sizes.These assemblies display distinctive photophysical behaviors that have been exploited for biosensing,bioimaging,and therapeutic applications in both cellular and in vivo models.Compared with individual AuNCs,assembled systems offer improved uptake,prolonged circulation,and efficient clearance,while protecting labile cargos such as nucleic acids and proteins.Moreover,their ordered and defined architectures can be engineered for controlled drug release and synergistic photo-or radiotherapeutic effects.Despite these advances,fundamental understanding of how structural organization governs photophysical responses remains limited.Elucidating parameters such as intercluster spacing and loading density will be essential for optimizing performance.Overall,biologically guided AuNC assemblies represent a powerful platform for multifunctional biosensing and therapy,bridging nanoscale design with biological function.展开更多
The United Nations Sustainable Development Goal(SDG) 2 aims to achieve Zero Hunger by 2030.However,global hunger and food insecurity have continued to rise at an alarming rate(UN 2023).Subtropical regions are home to ...The United Nations Sustainable Development Goal(SDG) 2 aims to achieve Zero Hunger by 2030.However,global hunger and food insecurity have continued to rise at an alarming rate(UN 2023).Subtropical regions are home to more than 30% of the world's population,predominantly in developing countries where per capita farmland and food supply are only 40% of those in developed nations(FAO 2018).Meeting the Zero Hunger target amid ongoing population growth in these regions requires a substantial increase in agricultural production while minimizing soil degradation and adverse ecological impacts.This challenge is shared by many countries across South Asia,Africa,and Central and South America.展开更多
Innovation and Development Policy,简称“IDP”,中文名:《创新与发展政策(英文)》,是由中国科学院主管,中国科学院科技战略咨询研究院、中国科学学与科技政策研究会共同主办,国内外发行(ISSN:2096-5141,CN:10-1513/D)的英文国际期刊...Innovation and Development Policy,简称“IDP”,中文名:《创新与发展政策(英文)》,是由中国科学院主管,中国科学院科技战略咨询研究院、中国科学学与科技政策研究会共同主办,国内外发行(ISSN:2096-5141,CN:10-1513/D)的英文国际期刊。目前期刊每年出版两期。已发表论文在期刊网站可以自由获取,http://idp-journal.casisd.cn/browse/al/al2019/。展开更多
Innovation and Development Policy简称“IDP”,中文名《创新与发展政策(英文)》,是由中国科学院主管,中国科学院科技战略咨询研究院、中国科学学与科技政策研究会共同主办,国内外发行(ISSN:2096-5141,CN:10-1513/D)的英文国际期刊。...Innovation and Development Policy简称“IDP”,中文名《创新与发展政策(英文)》,是由中国科学院主管,中国科学院科技战略咨询研究院、中国科学学与科技政策研究会共同主办,国内外发行(ISSN:2096-5141,CN:10-1513/D)的英文国际期刊。目前期刊每年出版四期。已发表论文在期刊网站可以自由获取,http://idp-journal.casisd.cn/browse/al/al2019/。展开更多
High-temperature-annealed Al N(HTA-Al N) templates provide ideal substrates for high-quality Al Ga N epitaxy. However, the significant compressive stress accumulated within the Al Ga N layer makes it challenging to ac...High-temperature-annealed Al N(HTA-Al N) templates provide ideal substrates for high-quality Al Ga N epitaxy. However, the significant compressive stress accumulated within the Al Ga N layer makes it challenging to achieve a smooth surface free of hexagonal hillocks on these templates. To address this issue, we investigate the mechanism of compressive stress accumulation during the growth of Al Ga N-based epilayers on HTA-Al N templates using in-situ curvature analysis in this study. To verify the mechanism, a low-Al-content Al Ga N interlayer is introduced between the Al N epilayer and the subsequent Al Ga N epilayer. The larger a-plane lattice constant of this interlayer relative to the Al Ga N epilayer slows the accumulation rate of compressive stress. The hexagonal hillock can be effectively suppressed and the surface of Al Ga N epilayer can be significantly regulated by adopting various low-Al-content Al Ga N interlayers. This work provides a comprehension on the stress accumulation mechanism in Al Ga N epilayers and a feasible method to obtain hillock-free surface of Al Ga N epilayers on HTA-Al N templates,which will be beneficial for fabricating Al Ga N based devices.展开更多
Standards are the common language that consolidates global consensus and builds the most solid foundation for international partnerships.They are the cornerstone for global sustainable and high-quality development.You...Standards are the common language that consolidates global consensus and builds the most solid foundation for international partnerships.They are the cornerstone for global sustainable and high-quality development.Young students,with their active and vibrant minds,represent the future and hope of standardization.展开更多
China is carving out a distinctive development path which features urban-rural integration.This approach has not only yielded tangible results domestically but also drawn the attention of other countries.
County economies form a distinctive,integrated regional economic structure.Their growth is shaped by specific social and historical conditions and,in turn,influences many aspects of wider economic and social developme...County economies form a distinctive,integrated regional economic structure.Their growth is shaped by specific social and historical conditions and,in turn,influences many aspects of wider economic and social development.In China's modernization process,they have always played a vital role.The 2024 Central Economic Work Conference placed strong emphasis on vigorously developing county economies.展开更多
Time flows with no stop,and seasons change in swift succession.As we bid farewell to 2025 and welcome the Spring Festival of 2026,the Editorial Office of Petroleum Exploration and Development extends our most heartfel...Time flows with no stop,and seasons change in swift succession.As we bid farewell to 2025 and welcome the Spring Festival of 2026,the Editorial Office of Petroleum Exploration and Development extends our most heartfelt gratitude and sincerest New Year wishes to our editorial board members,experts,authors,readers,and friends from all sectors.展开更多
To advance intelligent construction,standards must come first.The Ministry of Housing and Urban-Rural Development has issued the List for Replicable Experience and Practices for Developing Intelligent Construction fou...To advance intelligent construction,standards must come first.The Ministry of Housing and Urban-Rural Development has issued the List for Replicable Experience and Practices for Developing Intelligent Construction four times successively and the Technical Guidelines for Intelligent Construction(Trial).展开更多
1.Background The United Nations(UN)2030 Agenda for Sustainable Development,adopted in 2015,established the Sustainable Development Goals(SDGs)as a comprehensive framework to address global challenges through interconn...1.Background The United Nations(UN)2030 Agenda for Sustainable Development,adopted in 2015,established the Sustainable Development Goals(SDGs)as a comprehensive framework to address global challenges through interconnected social,economic,and environmental targets.展开更多
China aims to build on its achievements of the past five years to accelerate rural revitalization during the 15th Five-Year Plan period.IN China,issues relating to agriculture,rural areas,and rural residents have alwa...China aims to build on its achievements of the past five years to accelerate rural revitalization during the 15th Five-Year Plan period.IN China,issues relating to agriculture,rural areas,and rural residents have always been a priority in national development.At its fourth plenary session held in October 2025,the 20th Central Committee of the Communist Party of China(CPC)once again emphasized the need to accelerate agricultural and rural modernization,and advance all-around rural revitalization over the next five years.展开更多
Neurodevelopmental processes represent a finely tuned interplay between genetic and environmental factors,shaping the dynamic landscape of the developing brain.A major component of the developing brain that enables th...Neurodevelopmental processes represent a finely tuned interplay between genetic and environmental factors,shaping the dynamic landscape of the developing brain.A major component of the developing brain that enables this dynamic is the white matter(WM),known to be affected in neurodevelopmental disorders(NDDs)(Rokach et al.,2024).WM formation is mediated by myelination,a multifactorial process driven by neuro-glia interactions dependent on proper neuronal functionality(Simons and Trajkovic,2006).Another key aspect of neurodevelopmental abnormalities involves neuronal dynamics and function,with recent advances significantly enhancing our understanding of both neuronal and glial mitochondrial function(Devine and Kittler,2018;Rojas-Charry et al.,2021).Energy homeostasis in neurons,attributed largely to mitochondrial function,is critical for proper functionality and interactions with oligodendrocytes(OLs),the cells forming myelin in the brain’s WM.We herein discuss the interplay between these processes and speculate on potential dysfunction in NDDs.展开更多
Environmental DNA(eDNA)technology has revolutionized biodiversity monitoring with its non-invasive,sensitive,and cost-efficient approach.This paper systematically reviews eDNA advancements,examining its applications i...Environmental DNA(eDNA)technology has revolutionized biodiversity monitoring with its non-invasive,sensitive,and cost-efficient approach.This paper systematically reviews eDNA advancements,examining its applications in aquatic and terrestrial ecosystems and assessing China’s standardization progress.It delineates four developmental phases from single-species detection to high-throughput sequencing,and highlights China’s contribution to the development of technical standards.While significant progress has been made,challenges persist in quantitative accuracy,methodological consistency,and large-scale implementation.Future efforts should prioritize enhanced standardization,improved quantification techniques,broader applications,and international collaboration to drive innovation in eDNA technology.展开更多
After the end of World War II,African countries successively gained independence and ushered in a golden age of economic development.Through the strategies of primary product exports,transitional economic policies and...After the end of World War II,African countries successively gained independence and ushered in a golden age of economic development.Through the strategies of primary product exports,transitional economic policies and import substitution development,their economic growth accelerated significantly.However,due to the limitations of these strategies,the structural problem of Africa being an exporter of cheap industrial raw materials and an importer of manufactured goods remained unresolved.展开更多
The 20th Communist Party of China(CPC)Central Committee held its fourth plenary session in Beijing on October 20-23.The plenary session's central task was to deliberate on the framework of the country's next n...The 20th Communist Party of China(CPC)Central Committee held its fourth plenary session in Beijing on October 20-23.The plenary session's central task was to deliberate on the framework of the country's next national development roadmap:the 15th Five-Year Plan(2026-2030).Every five-year plan is important but this one arrives at a truly pivotal moment in terms of China's development trajectory and the global environment.展开更多
The development of skeletal muscle are complicated processes involving genes responsible for proper muscle morphology,contractility,cell proliferation,differentiation,interactions,migration,and death.The three-dimensi...The development of skeletal muscle are complicated processes involving genes responsible for proper muscle morphology,contractility,cell proliferation,differentiation,interactions,migration,and death.The three-dimensional chromatin architecture of skeletal muscle development has not been studied intensively although dynamic transcriptional regulation during differentiation of muscle cells is one of the most deeply studied processes.The RNA-seq was used to analyze the transcriptome pattern during chicken muscle development across 12 stages.Hi-C was used to build chromatin architectures during four representative stages.Ch IP-seq was conducted to identify enhancers and promoters in these four stages,which are occupied by histone H3K27ac and H3K4me3 peaks.Results show that large-scale genome architecture changes are mostly unidirectional,and coupled by complex on/off dynamic patterns of gene expression.Specifically,we observed 258.30 Mb of the genome undergoing A/B compartment switching.Notable alterations(316.57 Mb)of interaction frequencies within TADs were observed.Substantial aging-associated genes exhibited ascending connectivity with the compartment transition from repressive to active status during muscle development.Some muscle-related gene promoters that interacted with active enhancers during development,and some myopathy/aging-associated genes that were activated in aging muscle were founded.These results provide key insights into skeletal muscle development in vivo,and offer a valuable resource that allows in-depth functional characterization of candidate genes.展开更多
With the advent of the big data era,modern statistics has enjoyed unprecedented development opportunities and also faced numerous new challenges.Traditional statistical computing methods are often limited by issues su...With the advent of the big data era,modern statistics has enjoyed unprecedented development opportunities and also faced numerous new challenges.Traditional statistical computing methods are often limited by issues such as computer memory capacity and distributed storage of data across different locations,and are unable to directly apply to large-scale data sets.Therefore,in the context of big data,designing efficient and theoretically guaranteed statistical learning and inference algorithms has become a key issue that the current field of statistics urgently needs to address.In this paper,the application status of statistical analysis methods in the big data environment was systematically reviewed,and its future development directions were analyzed to provide reference and support for the further development of theory and methods of the statistical analysis of big data.展开更多
Against the backdrop of China s accelerated green development and its pursuit of the"dual carbon"goals,green finance,as a crucial instrument for the rational allocation of resources,plays a significant role ...Against the backdrop of China s accelerated green development and its pursuit of the"dual carbon"goals,green finance,as a crucial instrument for the rational allocation of resources,plays a significant role in promoting agricultural green transformation.Taking Henan Province as a case study,this research employs panel data from 18 prefecture-level cities spanning 2014 to 2023,utilizing dynamic panel models and mediating effect models for empirical analysis.The findings reveal that green finance indirectly fosters agricultural green development by driving agricultural technological progress.This promoting effect is significant,with the mediating effect accounting for 3.67%.Based on these results,policy recommendations are proposed:expanding the coverage of green finance and constructing a diversified product system;comprehensively promoting agricultural technological innovation and mechanization development;and coordinating and integrating green financial resources to facilitate agricultural green transformation tailored to local conditions.展开更多
基金supported by grants from Simons Foundation (SFARI 479754),CIHR (PJT-180565)the Scottish Rite Charitable Foundation of Canada (to YL)funding from the Canada Research Chairs program。
文摘The mechanistic target of rapamycin(m TOR) is a serine/threonine kinase that plays a pivotal role in cellular growth, proliferation, survival, and metabolism. In the central nervous system(CNS), the mTOR pathway regulates diverse aspects of neural development and function. Genetic mutations within the m TOR pathway lead to severe neurodevelopmental disorders, collectively known as “mTORopathies”(Crino, 2020). Dysfunctions of m TOR, including both its hyperactivation and hypoactivation, have also been implicated in a wide spectrum of other neurodevelopmental and neurodegenerative conditions, highlighting its importance in CNS health.
基金NR SEQUOIA(ANR-22-CE18-0006)Nan0Gold(ANR-22-CE29-0022)+3 种基金SAMURAI(ANR-24-CE19-2073-01)Wilive(ANR-24-CE09-2351-03)EUR CBH-EUR-GS(ANR-17-EURE 0003)for their financial supportthe French National Research Agency(Labex ARCANE,ANR-11-LABX-003 and CBH-EUR-GS,ANR-17-EURE-0003)that supported part of this study.
文摘Gold nanoclusters(AuNCs)are ultrasmall(<2 nm)aggregates of gold atoms that exhibit discrete electronic states,size-dependent photoluminescence,and exceptional biocompatibility,making them ideal candidates for theranostic applications.Their tunable surface chemistry enables targeted delivery,while strong near-infrared emission and environmental responsiveness allow for sensitive detection and deep-tissue imaging.Recent advances have revealed that controlled assembly of AuNCs into higher-order architectures-guided by biological scaffolds such as nucleic acids,peptides,and proteins-can markedly enhance their optical and electronic properties through aggregation-induced emission(AiE)and stabilization of surface ligands.This review summarizes recent progress in the design and biomedical applications of AuNC assemblies generated using biomolecules as structure-directing scaffolds.Covalent and noncovalent interactions with biomolecules enable the formation of well-defined one-,two-,and three-dimensional structures with tunable morphologies and sizes.These assemblies display distinctive photophysical behaviors that have been exploited for biosensing,bioimaging,and therapeutic applications in both cellular and in vivo models.Compared with individual AuNCs,assembled systems offer improved uptake,prolonged circulation,and efficient clearance,while protecting labile cargos such as nucleic acids and proteins.Moreover,their ordered and defined architectures can be engineered for controlled drug release and synergistic photo-or radiotherapeutic effects.Despite these advances,fundamental understanding of how structural organization governs photophysical responses remains limited.Elucidating parameters such as intercluster spacing and loading density will be essential for optimizing performance.Overall,biologically guided AuNC assemblies represent a powerful platform for multifunctional biosensing and therapy,bridging nanoscale design with biological function.
文摘The United Nations Sustainable Development Goal(SDG) 2 aims to achieve Zero Hunger by 2030.However,global hunger and food insecurity have continued to rise at an alarming rate(UN 2023).Subtropical regions are home to more than 30% of the world's population,predominantly in developing countries where per capita farmland and food supply are only 40% of those in developed nations(FAO 2018).Meeting the Zero Hunger target amid ongoing population growth in these regions requires a substantial increase in agricultural production while minimizing soil degradation and adverse ecological impacts.This challenge is shared by many countries across South Asia,Africa,and Central and South America.
文摘Innovation and Development Policy,简称“IDP”,中文名:《创新与发展政策(英文)》,是由中国科学院主管,中国科学院科技战略咨询研究院、中国科学学与科技政策研究会共同主办,国内外发行(ISSN:2096-5141,CN:10-1513/D)的英文国际期刊。目前期刊每年出版两期。已发表论文在期刊网站可以自由获取,http://idp-journal.casisd.cn/browse/al/al2019/。
文摘Innovation and Development Policy简称“IDP”,中文名《创新与发展政策(英文)》,是由中国科学院主管,中国科学院科技战略咨询研究院、中国科学学与科技政策研究会共同主办,国内外发行(ISSN:2096-5141,CN:10-1513/D)的英文国际期刊。目前期刊每年出版四期。已发表论文在期刊网站可以自由获取,http://idp-journal.casisd.cn/browse/al/al2019/。
基金supported by the National Key R&D Program of China (Grant No. 2022YFB3605000)the National Natural Science Foundation of China (Nos. 62004127, 61725403, 62121005, 61922078, 61827813, and 62004196)+1 种基金the Youth Innovation Promotion Association of Chinese Academy of Sciencesthe Youth Talent Promotion Project of the Chinese Institute of Electronics (No. 2020QNRC001)。
文摘High-temperature-annealed Al N(HTA-Al N) templates provide ideal substrates for high-quality Al Ga N epitaxy. However, the significant compressive stress accumulated within the Al Ga N layer makes it challenging to achieve a smooth surface free of hexagonal hillocks on these templates. To address this issue, we investigate the mechanism of compressive stress accumulation during the growth of Al Ga N-based epilayers on HTA-Al N templates using in-situ curvature analysis in this study. To verify the mechanism, a low-Al-content Al Ga N interlayer is introduced between the Al N epilayer and the subsequent Al Ga N epilayer. The larger a-plane lattice constant of this interlayer relative to the Al Ga N epilayer slows the accumulation rate of compressive stress. The hexagonal hillock can be effectively suppressed and the surface of Al Ga N epilayer can be significantly regulated by adopting various low-Al-content Al Ga N interlayers. This work provides a comprehension on the stress accumulation mechanism in Al Ga N epilayers and a feasible method to obtain hillock-free surface of Al Ga N epilayers on HTA-Al N templates,which will be beneficial for fabricating Al Ga N based devices.
文摘Standards are the common language that consolidates global consensus and builds the most solid foundation for international partnerships.They are the cornerstone for global sustainable and high-quality development.Young students,with their active and vibrant minds,represent the future and hope of standardization.
文摘China is carving out a distinctive development path which features urban-rural integration.This approach has not only yielded tangible results domestically but also drawn the attention of other countries.
文摘County economies form a distinctive,integrated regional economic structure.Their growth is shaped by specific social and historical conditions and,in turn,influences many aspects of wider economic and social development.In China's modernization process,they have always played a vital role.The 2024 Central Economic Work Conference placed strong emphasis on vigorously developing county economies.
文摘Time flows with no stop,and seasons change in swift succession.As we bid farewell to 2025 and welcome the Spring Festival of 2026,the Editorial Office of Petroleum Exploration and Development extends our most heartfelt gratitude and sincerest New Year wishes to our editorial board members,experts,authors,readers,and friends from all sectors.
文摘To advance intelligent construction,standards must come first.The Ministry of Housing and Urban-Rural Development has issued the List for Replicable Experience and Practices for Developing Intelligent Construction four times successively and the Technical Guidelines for Intelligent Construction(Trial).
文摘1.Background The United Nations(UN)2030 Agenda for Sustainable Development,adopted in 2015,established the Sustainable Development Goals(SDGs)as a comprehensive framework to address global challenges through interconnected social,economic,and environmental targets.
文摘China aims to build on its achievements of the past five years to accelerate rural revitalization during the 15th Five-Year Plan period.IN China,issues relating to agriculture,rural areas,and rural residents have always been a priority in national development.At its fourth plenary session held in October 2025,the 20th Central Committee of the Communist Party of China(CPC)once again emphasized the need to accelerate agricultural and rural modernization,and advance all-around rural revitalization over the next five years.
文摘Neurodevelopmental processes represent a finely tuned interplay between genetic and environmental factors,shaping the dynamic landscape of the developing brain.A major component of the developing brain that enables this dynamic is the white matter(WM),known to be affected in neurodevelopmental disorders(NDDs)(Rokach et al.,2024).WM formation is mediated by myelination,a multifactorial process driven by neuro-glia interactions dependent on proper neuronal functionality(Simons and Trajkovic,2006).Another key aspect of neurodevelopmental abnormalities involves neuronal dynamics and function,with recent advances significantly enhancing our understanding of both neuronal and glial mitochondrial function(Devine and Kittler,2018;Rojas-Charry et al.,2021).Energy homeostasis in neurons,attributed largely to mitochondrial function,is critical for proper functionality and interactions with oligodendrocytes(OLs),the cells forming myelin in the brain’s WM.We herein discuss the interplay between these processes and speculate on potential dysfunction in NDDs.
基金supported by the National Natural Science Foundation of China(Grant No.32160172)the Key Science-Technology Project of Inner Mongolia(2023KYPT0010)+1 种基金the Natural Science Foundation of Inner Mongolia Autonomous Region of China(Grant No.2025QN03006)the 2023 Inner Mongolia Public Institution High-Level Talent Introduction Scientific Research Support Project.
文摘Environmental DNA(eDNA)technology has revolutionized biodiversity monitoring with its non-invasive,sensitive,and cost-efficient approach.This paper systematically reviews eDNA advancements,examining its applications in aquatic and terrestrial ecosystems and assessing China’s standardization progress.It delineates four developmental phases from single-species detection to high-throughput sequencing,and highlights China’s contribution to the development of technical standards.While significant progress has been made,challenges persist in quantitative accuracy,methodological consistency,and large-scale implementation.Future efforts should prioritize enhanced standardization,improved quantification techniques,broader applications,and international collaboration to drive innovation in eDNA technology.
文摘After the end of World War II,African countries successively gained independence and ushered in a golden age of economic development.Through the strategies of primary product exports,transitional economic policies and import substitution development,their economic growth accelerated significantly.However,due to the limitations of these strategies,the structural problem of Africa being an exporter of cheap industrial raw materials and an importer of manufactured goods remained unresolved.
文摘The 20th Communist Party of China(CPC)Central Committee held its fourth plenary session in Beijing on October 20-23.The plenary session's central task was to deliberate on the framework of the country's next national development roadmap:the 15th Five-Year Plan(2026-2030).Every five-year plan is important but this one arrives at a truly pivotal moment in terms of China's development trajectory and the global environment.
基金supported by the National Key R&D Program of China(2023YFD1300040 and 2022YFF1000100)the Sichuan Science and Technology Program,China(2022NSFSC0132,2021YFYZ0009 and 2022JDJQ0054)the National Natural Science Foundation of China(32225046)。
文摘The development of skeletal muscle are complicated processes involving genes responsible for proper muscle morphology,contractility,cell proliferation,differentiation,interactions,migration,and death.The three-dimensional chromatin architecture of skeletal muscle development has not been studied intensively although dynamic transcriptional regulation during differentiation of muscle cells is one of the most deeply studied processes.The RNA-seq was used to analyze the transcriptome pattern during chicken muscle development across 12 stages.Hi-C was used to build chromatin architectures during four representative stages.Ch IP-seq was conducted to identify enhancers and promoters in these four stages,which are occupied by histone H3K27ac and H3K4me3 peaks.Results show that large-scale genome architecture changes are mostly unidirectional,and coupled by complex on/off dynamic patterns of gene expression.Specifically,we observed 258.30 Mb of the genome undergoing A/B compartment switching.Notable alterations(316.57 Mb)of interaction frequencies within TADs were observed.Substantial aging-associated genes exhibited ascending connectivity with the compartment transition from repressive to active status during muscle development.Some muscle-related gene promoters that interacted with active enhancers during development,and some myopathy/aging-associated genes that were activated in aging muscle were founded.These results provide key insights into skeletal muscle development in vivo,and offer a valuable resource that allows in-depth functional characterization of candidate genes.
文摘With the advent of the big data era,modern statistics has enjoyed unprecedented development opportunities and also faced numerous new challenges.Traditional statistical computing methods are often limited by issues such as computer memory capacity and distributed storage of data across different locations,and are unable to directly apply to large-scale data sets.Therefore,in the context of big data,designing efficient and theoretically guaranteed statistical learning and inference algorithms has become a key issue that the current field of statistics urgently needs to address.In this paper,the application status of statistical analysis methods in the big data environment was systematically reviewed,and its future development directions were analyzed to provide reference and support for the further development of theory and methods of the statistical analysis of big data.
基金Supported by Zhejiang Provincial Philosophy and Social Sciences Planning Project(23FNSQ58YB).
文摘Against the backdrop of China s accelerated green development and its pursuit of the"dual carbon"goals,green finance,as a crucial instrument for the rational allocation of resources,plays a significant role in promoting agricultural green transformation.Taking Henan Province as a case study,this research employs panel data from 18 prefecture-level cities spanning 2014 to 2023,utilizing dynamic panel models and mediating effect models for empirical analysis.The findings reveal that green finance indirectly fosters agricultural green development by driving agricultural technological progress.This promoting effect is significant,with the mediating effect accounting for 3.67%.Based on these results,policy recommendations are proposed:expanding the coverage of green finance and constructing a diversified product system;comprehensively promoting agricultural technological innovation and mechanization development;and coordinating and integrating green financial resources to facilitate agricultural green transformation tailored to local conditions.
