Objective To report Medtronic experiences with the development of animal models for atrial fibrillation （AF） and chronic heart failure （CHF） using high-rate pacing for AF and microemboli for CHF. Methods For the A...Objective To report Medtronic experiences with the development of animal models for atrial fibrillation （AF） and chronic heart failure （CHF） using high-rate pacing for AF and microemboli for CHF. Methods For the AF model, an atrial lead was attached to a Medtronic SynergyTM neurostimulator, which was programmed to stimulate at 50 Hz in an on-off duty cycle. Atrial natfiuretic peptide （ANP）, brain natriuretic peptide （BNP） and N-terminal pro brain nalriuretic peptide （NT-proBNP） were assayed at select time points. For CHF model, a serial injection of 90 μm polystyrene microspheres at 62,400 beads/mL （Polybead, Polysciences, Inc.） was performed to induce global ischemia, either with weekly monitoring and embolization schedule （group 1, n = 25） or with biweekly monitoring and emboliation schedule （group 2, n = 36 ）. Echocardiograms were used along with ventriculograms and magnetic resonance imaging scans weekly to assess cardiac function and ANP, BNP and NT-proBNP were monitored. Results For the AF model, the days to sustained AF for four animals following surgery were 7, 25, 21 and 19, respectively; For the CHF model, the days to meet CHF endpoints were 116 in group 1 and 89 in group 2. For both AF and CHF models, NT-proBNP correlated well with the development of disease states. Conclusion Our experience for the development and assessment of AF and CHF dog models may help researchers who are in search for animal model for assessing the safety and efficacy of a device-based therapy.展开更多
The oculomotor nerves of beagle dogs received electrical stimulation at 0.3-2.0 V. After recording compound muscle action potentials of the inferior oblique muscle, the oculomotor nerve was quickly cut off and a direc...The oculomotor nerves of beagle dogs received electrical stimulation at 0.3-2.0 V. After recording compound muscle action potentials of the inferior oblique muscle, the oculomotor nerve was quickly cut off and a direct end-to-end anastomosis was then performed. As a result, the stimulating elec-trode was smoothly inserted and placed, and ideal bioelectrical signals of the interior oblique muscle were acquired. After oculomotor nerve injury, compound muscle action potentials of the inferior oblique muscle were significantly decreased in beagle dogs. These findings suggest that an animal model of oculomotor nerve injury was successfully established for electrophysiological studies.展开更多
BACKGROUND： At present, suppurative meningitis is mainly treated through anti-infection with antibiotics, depressing encephalic pressure with mannitol , lowering body temperature with drugs , supporting treatment, et...BACKGROUND： At present, suppurative meningitis is mainly treated through anti-infection with antibiotics, depressing encephalic pressure with mannitol , lowering body temperature with drugs , supporting treatment, etc. However, Jt takes a long course of treatment and has poor therapeutic effect. Successive irrigation of subarachnoid cavity maybe have better effect on suppurative meningitis.OBJECTIVE： We compared the successive irrigation of subbarachnoid cavity with routine therapeutic methods to observe the effect of successive irrigation of subarachnoid cavity on the body temperature, cerebrospinal fluid pressure, the number of white blood cell and the level of protein of suppurative meningitis dogs. DESIGN： A randomized and controlled animal experiment SETTING： Institute of Neuroscience, Taihe Hospital Affiliated to Yunyang Medical College MATERIALS： Totally 17 healthy adult male Beagle dogs, of common grade, weighing 9 to 10 kg, were involved in the experiment, and raised in the 20 ℃ temperature with relative humidity of 50% for 1 week. They were randomized into 3 groups： normal group （n=5）, control group （n=5） and irrigation group （n=6）. Artificial cerebrospinal fluid was prepared according to the level of glucose and chloride of cerebrospinal fluid of normal dogs, and then it was sterilized with high pressure. METHODS ： This experiment was carried out in the experimental animal center of Yunyang Medical College from April to August 2001. ① After the dogs were anesthetized,1 mL fresh staphylococcus aureus liquid [（1.5-1.6）× 10^9 L 1] was injected into medullary cistern to establish suppurative meningitis models. ② After models were successfully established, intravenous drip infusion of 1.2 ×10^6 U/（kg.d）, muscular injection of sulfadiazine sodium of 100 mg/（kg.d ）and intravenous injection of 200 g/L mannitol of 5 g/（kg .time） for 3 times a day were performed in the control group. The irrigation of subarachnoid meningitis was conducted in the irrigation group besides the routine treatments in the control group： Artificial cerebrospinal fluid was successively injected into the epidural catheter in the waist part. The mixtures of artificial cerebrospinal fluid and inflammatory cerebrospinal fluid flowed out incessantly from epidural catheter of medullary cistern. Irrigation was ended 48 hours later. Dogs were routinely raised in the control group. ③ From the first day after being modeled to the 14^th day after treatment, the rectal temperatures were daily measured in the morning, at noon and in the evening respectively, then the mean temperatures were calculated. From the 2^nd day after being modeled to the 14^th day after treatment, puncture at waist was given once, and the cerebrospinal fluid pressure, the number of white blood cells and the level of protein were measured. MAIN OUTCOME MEASURES ： The body temperature, cerebrospinal fluid pressure, the number of white blood cells and the level of protein were measured on suppurative meningitis model dogs. RESULTS＂ Totally 17 Beagle dogs were involved in this study.①The body temperatures of dogs were significantly reduced in the control group and in irrigation group on the 1^st day after treatment and lower than suppurative meningitis model dogs on the 2^nd day after being modeled （P 〈 0.05）. The body temperatures were gradually decreased in control group from the 2^nd day after being modeled to the 14^th day after treatment （P 〈 0.01）, but were significantly higher than those of dogs in normal group on the 14^th day after treatment （P 〈 0.05）. The body temperatures of dogs were gradually decreased in irrigation group on the 2^nd day or 3^rd day after treatment （P 〈 0.01） and then were close to the normal body temperature on the 14^th day after treatment. ②There was the same cerebrospinal fluid pressure in control group as in irrigation group on the 2^nd day after being modeled （P 〉 0.05）, but significantly decreased in two groups from the 1s＇ day or 2^nd day after treatment （P 〈 0.01）, and cerebrospinal fluid pressure was close to the normal level in irrigation group. Although the cerebrospinal fluid pressure raised a little on the 3^rd day or 4^th day after treatment in the irrigation group （P〈 0.05）, but recovered to the normal level on the 9^th day after treatment again. It decreased slowly in the control group and still was sig- nificantly higher than normal group on the 14^hd day after treatment （P〈 0.01 ）. ③No significant difference existed in number of white blood cell and the level of protein between control group and irrigation group on the 2^nd day following modeled. Both of them began to significantly decrease on the 1^st and 2^nd day after treatment （P 〈 0.01 ）. Although the number of white blood cell and the level of protein rebounded a little in irrigation group 3 or 4 days after treatment （P〈 0.05）, they were gradually decreased and recovered to normal levels on the 13^th day after treatment. The number of white blood cell and the level of protein slowly decreased in the control group compared with irrigation group and were still significantly higher than normal group on the 14^th day （P〈 0.01 ）. EONELUSEON ： Irrigation of subarachnoid cavity can decrease the body temperature, cerebrospinal fluid pressure, the number of white blood cells and the level of protein at early stage of suppurative meningitis, and the therapeutic effect is better than that of routine treatment.展开更多
Many studies have been done and many results have been established for studying cancers in human and the ways of treating it. However, one thing that remains relevant is the study model that is used to diagnose, cure ...Many studies have been done and many results have been established for studying cancers in human and the ways of treating it. However, one thing that remains relevant is the study model that is used to diagnose, cure or conclude treatment methods for human cancers. The scientists have tried some ways to link the data and tried to analyze the malicious disease in various animal models in order to solve the problem for humans. Out of all the models, scientists have preferred dogs as the most suitable model and conducted studies on them. Our article will review the reason for preferences given to dog as a study model and what the previous studies have tried to conclude by considering the dreaded disease in dogs. Our article has focused on most of the recent observations and tried to elucidate the reasons/preferences for studying cancer disease in dogs (scientific name;Canis Lupus familiaris). We will also talk about the idea of comparative oncology programs that many centers adapt in order to study the disease called cancer.展开更多
AIM: we recommend a method of Simple auto-oLT model in dogs.METHODS:The model was ligated all ligaments or connective tissues of the liver,only reserved the vascular construction,that was the suprahepatic and infrahep...AIM: we recommend a method of Simple auto-oLT model in dogs.METHODS:The model was ligated all ligaments or connective tissues of the liver,only reserved the vascular construction,that was the suprahepatic and infrahepatic inferior vena cava,portal vein,hepatic artery or common bile duct.the operation was similar to the orthotopic liver transplantation except vascular anastomoses,the dog liver underwent the warm or cold ischemia and the reperfusated injurous process.RESULTS: The imitability was exactly good and the operation was simple and safe. Because the hepatic vessels of the going out or coming in was clamped block and might open or blind the blood flow whenever necessary,the model might control the warm or cold ischemic time accurately,and eliminate the influence or the complications due to vascular anastomoses.CONCLUSIONS: The model avoided many-sided Influences of the traditional OLT and was a good method to study hepatic artery or portal vein ischemic injury and created a new way to explore the pathogenesis or some complications in the OLT.展开更多
Objective We expect to have a profound understanding of refractory high intracranial pressure after traumatic brain injury by making a reproducible animal model in dogs experimentally. Methods Ten adult dogs were used...Objective We expect to have a profound understanding of refractory high intracranial pressure after traumatic brain injury by making a reproducible animal model in dogs experimentally. Methods Ten adult dogs were used to make acute epidural balloon compression and decompression models.展开更多
Functional recovery after oculomotor nerve injury is very poor. Electrical stimulation has been shown to promote regeneration of injured nerves. We hypothesized that electrical stimulation would improve the functional...Functional recovery after oculomotor nerve injury is very poor. Electrical stimulation has been shown to promote regeneration of injured nerves. We hypothesized that electrical stimulation would improve the functional recovery of injured oculomotor nerves. Oculomotor nerve injury models were created by crushing the right oculomotor nerves of adult dogs. Stimulating electrodes were positioned in both proximal and distal locations of the lesion, and non-continuous rectangular, biphasic current pulses （0.7 V, 5 Hz） were administered 1 hour daily for 2 consecutive weeks. Analysis of the results showed that electrophysiological and morphological recovery of the injured oc- ulomotor nerve was enhanced, indicating that electrical stimulation improved neural regeneration. Thus, this therapy has the potential to promote the recovery of oculomotor nerve dysfunction.展开更多
文摘Objective To report Medtronic experiences with the development of animal models for atrial fibrillation （AF） and chronic heart failure （CHF） using high-rate pacing for AF and microemboli for CHF. Methods For the AF model, an atrial lead was attached to a Medtronic SynergyTM neurostimulator, which was programmed to stimulate at 50 Hz in an on-off duty cycle. Atrial natfiuretic peptide （ANP）, brain natriuretic peptide （BNP） and N-terminal pro brain nalriuretic peptide （NT-proBNP） were assayed at select time points. For CHF model, a serial injection of 90 μm polystyrene microspheres at 62,400 beads/mL （Polybead, Polysciences, Inc.） was performed to induce global ischemia, either with weekly monitoring and embolization schedule （group 1, n = 25） or with biweekly monitoring and emboliation schedule （group 2, n = 36 ）. Echocardiograms were used along with ventriculograms and magnetic resonance imaging scans weekly to assess cardiac function and ANP, BNP and NT-proBNP were monitored. Results For the AF model, the days to sustained AF for four animals following surgery were 7, 25, 21 and 19, respectively; For the CHF model, the days to meet CHF endpoints were 116 in group 1 and 89 in group 2. For both AF and CHF models, NT-proBNP correlated well with the development of disease states. Conclusion Our experience for the development and assessment of AF and CHF dog models may help researchers who are in search for animal model for assessing the safety and efficacy of a device-based therapy.
基金the National Natural Science Foundation of China, No.30571907the Shanghai Natural Science Foundation, No.05QMH1409
文摘The oculomotor nerves of beagle dogs received electrical stimulation at 0.3-2.0 V. After recording compound muscle action potentials of the inferior oblique muscle, the oculomotor nerve was quickly cut off and a direct end-to-end anastomosis was then performed. As a result, the stimulating elec-trode was smoothly inserted and placed, and ideal bioelectrical signals of the interior oblique muscle were acquired. After oculomotor nerve injury, compound muscle action potentials of the inferior oblique muscle were significantly decreased in beagle dogs. These findings suggest that an animal model of oculomotor nerve injury was successfully established for electrophysiological studies.
文摘BACKGROUND： At present, suppurative meningitis is mainly treated through anti-infection with antibiotics, depressing encephalic pressure with mannitol , lowering body temperature with drugs , supporting treatment, etc. However, Jt takes a long course of treatment and has poor therapeutic effect. Successive irrigation of subarachnoid cavity maybe have better effect on suppurative meningitis.OBJECTIVE： We compared the successive irrigation of subbarachnoid cavity with routine therapeutic methods to observe the effect of successive irrigation of subarachnoid cavity on the body temperature, cerebrospinal fluid pressure, the number of white blood cell and the level of protein of suppurative meningitis dogs. DESIGN： A randomized and controlled animal experiment SETTING： Institute of Neuroscience, Taihe Hospital Affiliated to Yunyang Medical College MATERIALS： Totally 17 healthy adult male Beagle dogs, of common grade, weighing 9 to 10 kg, were involved in the experiment, and raised in the 20 ℃ temperature with relative humidity of 50% for 1 week. They were randomized into 3 groups： normal group （n=5）, control group （n=5） and irrigation group （n=6）. Artificial cerebrospinal fluid was prepared according to the level of glucose and chloride of cerebrospinal fluid of normal dogs, and then it was sterilized with high pressure. METHODS ： This experiment was carried out in the experimental animal center of Yunyang Medical College from April to August 2001. ① After the dogs were anesthetized,1 mL fresh staphylococcus aureus liquid [（1.5-1.6）× 10^9 L 1] was injected into medullary cistern to establish suppurative meningitis models. ② After models were successfully established, intravenous drip infusion of 1.2 ×10^6 U/（kg.d）, muscular injection of sulfadiazine sodium of 100 mg/（kg.d ）and intravenous injection of 200 g/L mannitol of 5 g/（kg .time） for 3 times a day were performed in the control group. The irrigation of subarachnoid meningitis was conducted in the irrigation group besides the routine treatments in the control group： Artificial cerebrospinal fluid was successively injected into the epidural catheter in the waist part. The mixtures of artificial cerebrospinal fluid and inflammatory cerebrospinal fluid flowed out incessantly from epidural catheter of medullary cistern. Irrigation was ended 48 hours later. Dogs were routinely raised in the control group. ③ From the first day after being modeled to the 14^th day after treatment, the rectal temperatures were daily measured in the morning, at noon and in the evening respectively, then the mean temperatures were calculated. From the 2^nd day after being modeled to the 14^th day after treatment, puncture at waist was given once, and the cerebrospinal fluid pressure, the number of white blood cells and the level of protein were measured. MAIN OUTCOME MEASURES ： The body temperature, cerebrospinal fluid pressure, the number of white blood cells and the level of protein were measured on suppurative meningitis model dogs. RESULTS＂ Totally 17 Beagle dogs were involved in this study.①The body temperatures of dogs were significantly reduced in the control group and in irrigation group on the 1^st day after treatment and lower than suppurative meningitis model dogs on the 2^nd day after being modeled （P 〈 0.05）. The body temperatures were gradually decreased in control group from the 2^nd day after being modeled to the 14^th day after treatment （P 〈 0.01）, but were significantly higher than those of dogs in normal group on the 14^th day after treatment （P 〈 0.05）. The body temperatures of dogs were gradually decreased in irrigation group on the 2^nd day or 3^rd day after treatment （P 〈 0.01） and then were close to the normal body temperature on the 14^th day after treatment. ②There was the same cerebrospinal fluid pressure in control group as in irrigation group on the 2^nd day after being modeled （P 〉 0.05）, but significantly decreased in two groups from the 1s＇ day or 2^nd day after treatment （P 〈 0.01）, and cerebrospinal fluid pressure was close to the normal level in irrigation group. Although the cerebrospinal fluid pressure raised a little on the 3^rd day or 4^th day after treatment in the irrigation group （P〈 0.05）, but recovered to the normal level on the 9^th day after treatment again. It decreased slowly in the control group and still was sig- nificantly higher than normal group on the 14^hd day after treatment （P〈 0.01 ）. ③No significant difference existed in number of white blood cell and the level of protein between control group and irrigation group on the 2^nd day following modeled. Both of them began to significantly decrease on the 1^st and 2^nd day after treatment （P 〈 0.01 ）. Although the number of white blood cell and the level of protein rebounded a little in irrigation group 3 or 4 days after treatment （P〈 0.05）, they were gradually decreased and recovered to normal levels on the 13^th day after treatment. The number of white blood cell and the level of protein slowly decreased in the control group compared with irrigation group and were still significantly higher than normal group on the 14^th day （P〈 0.01 ）. EONELUSEON ： Irrigation of subarachnoid cavity can decrease the body temperature, cerebrospinal fluid pressure, the number of white blood cells and the level of protein at early stage of suppurative meningitis, and the therapeutic effect is better than that of routine treatment.
文摘Many studies have been done and many results have been established for studying cancers in human and the ways of treating it. However, one thing that remains relevant is the study model that is used to diagnose, cure or conclude treatment methods for human cancers. The scientists have tried some ways to link the data and tried to analyze the malicious disease in various animal models in order to solve the problem for humans. Out of all the models, scientists have preferred dogs as the most suitable model and conducted studies on them. Our article will review the reason for preferences given to dog as a study model and what the previous studies have tried to conclude by considering the dreaded disease in dogs. Our article has focused on most of the recent observations and tried to elucidate the reasons/preferences for studying cancer disease in dogs (scientific name;Canis Lupus familiaris). We will also talk about the idea of comparative oncology programs that many centers adapt in order to study the disease called cancer.
文摘AIM: we recommend a method of Simple auto-oLT model in dogs.METHODS:The model was ligated all ligaments or connective tissues of the liver,only reserved the vascular construction,that was the suprahepatic and infrahepatic inferior vena cava,portal vein,hepatic artery or common bile duct.the operation was similar to the orthotopic liver transplantation except vascular anastomoses,the dog liver underwent the warm or cold ischemia and the reperfusated injurous process.RESULTS: The imitability was exactly good and the operation was simple and safe. Because the hepatic vessels of the going out or coming in was clamped block and might open or blind the blood flow whenever necessary,the model might control the warm or cold ischemic time accurately,and eliminate the influence or the complications due to vascular anastomoses.CONCLUSIONS: The model avoided many-sided Influences of the traditional OLT and was a good method to study hepatic artery or portal vein ischemic injury and created a new way to explore the pathogenesis or some complications in the OLT.
文摘Objective We expect to have a profound understanding of refractory high intracranial pressure after traumatic brain injury by making a reproducible animal model in dogs experimentally. Methods Ten adult dogs were used to make acute epidural balloon compression and decompression models.
基金supported by a grant from the National Natural Science Foundation of China,No.30571907the International Science and Technology Cooperation Foundation of the Shanghai Committee of Science and Technology,China,No.10410711400
文摘Functional recovery after oculomotor nerve injury is very poor. Electrical stimulation has been shown to promote regeneration of injured nerves. We hypothesized that electrical stimulation would improve the functional recovery of injured oculomotor nerves. Oculomotor nerve injury models were created by crushing the right oculomotor nerves of adult dogs. Stimulating electrodes were positioned in both proximal and distal locations of the lesion, and non-continuous rectangular, biphasic current pulses （0.7 V, 5 Hz） were administered 1 hour daily for 2 consecutive weeks. Analysis of the results showed that electrophysiological and morphological recovery of the injured oc- ulomotor nerve was enhanced, indicating that electrical stimulation improved neural regeneration. Thus, this therapy has the potential to promote the recovery of oculomotor nerve dysfunction.
