Overview of the DNA damage response(DDR)in tumor cells.DDR is a highly coordinated signaling network that repairs DNA damage caused by intrinsic cellular processes and extrinsic insults,thereby preventing genome insta...Overview of the DNA damage response(DDR)in tumor cells.DDR is a highly coordinated signaling network that repairs DNA damage caused by intrinsic cellular processes and extrinsic insults,thereby preventing genome instability.Depending on the type of damage,distinct DNA damage repair and DNA damage tolerance(DDT)pathways are involved and coordinately regulated.展开更多
高通量DNA测序极大推动了碱基突变、基因拷贝数和结构变异等遗传变异事件的发现和检测。传统的高通量DNA测序(bulk DNA sequencing)所需的DNA来源于大量细胞,能检测到细胞群体共有的基因组变异事件,但会掩盖群体内单个细胞间的差异。为...高通量DNA测序极大推动了碱基突变、基因拷贝数和结构变异等遗传变异事件的发现和检测。传统的高通量DNA测序(bulk DNA sequencing)所需的DNA来源于大量细胞,能检测到细胞群体共有的基因组变异事件,但会掩盖群体内单个细胞间的差异。为了精准检测每个细胞间的差异,单细胞DNA测序(single cell DNA sequencing,scDNA-seq)技术被开发出来,极大推动了肿瘤基因组学、遗传学及疾病发生发展等学科领域的进展。该文简要概括了scDNA-seq技术的发展历程、面临的挑战、最新的进展及应用,并展望了scDNA-seq的未来发展方向。展开更多
Assembled protein-based substances are emerging and promising classes of materials that provide unique properties for various applications in biotechnology and nanotechnolegy. Self-assembly is an effective way to immo...Assembled protein-based substances are emerging and promising classes of materials that provide unique properties for various applications in biotechnology and nanotechnolegy. Self-assembly is an effective way to immobilize protein. In this study, DNAs-conjugated bovine serum albumin （BSA） assembled into fibers via DNA hybridization is demonstrated. The morphology of fibers was observed by optical microscopy, scanning electron microscopy （SEM）, and atomic force microscopy （AFM）, and the assembly mechanism was then analysed and discussed. BSA molecules were first linked by DNA molecule and formed linear chains. These chains then were parallelly linked through additional DNA hybridization. Finally, several BSA chains further assembled into fibers by layering lamellae in a parallel manner. This work perhaps will provide a guide to the immobilization of enzyme, which could be applied to increase its catalytic efficiency in biomedicine and nanotechnology.展开更多
基金the National Natural Science Foundation of China(Grant No.82330090 and Grant No.82341006 to C.G.)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDA0460403 to C.G.)the Natural Science Foundation of Shanxi Province(Grant No.202203021211155 to X.M.).
文摘Overview of the DNA damage response(DDR)in tumor cells.DDR is a highly coordinated signaling network that repairs DNA damage caused by intrinsic cellular processes and extrinsic insults,thereby preventing genome instability.Depending on the type of damage,distinct DNA damage repair and DNA damage tolerance(DDT)pathways are involved and coordinately regulated.
文摘高通量DNA测序极大推动了碱基突变、基因拷贝数和结构变异等遗传变异事件的发现和检测。传统的高通量DNA测序(bulk DNA sequencing)所需的DNA来源于大量细胞,能检测到细胞群体共有的基因组变异事件,但会掩盖群体内单个细胞间的差异。为了精准检测每个细胞间的差异,单细胞DNA测序(single cell DNA sequencing,scDNA-seq)技术被开发出来,极大推动了肿瘤基因组学、遗传学及疾病发生发展等学科领域的进展。该文简要概括了scDNA-seq技术的发展历程、面临的挑战、最新的进展及应用,并展望了scDNA-seq的未来发展方向。
基金supported by the National Natural Science Foundation of China(NSFC, No. 21174029)the Industry Academia Cooperation Innovation Fund of Jiangsu Province(No.BY201412707)+1 种基金the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)the Fundamental Research Funds for the Central Universities (No. 2242016K41020)
文摘Assembled protein-based substances are emerging and promising classes of materials that provide unique properties for various applications in biotechnology and nanotechnolegy. Self-assembly is an effective way to immobilize protein. In this study, DNAs-conjugated bovine serum albumin （BSA） assembled into fibers via DNA hybridization is demonstrated. The morphology of fibers was observed by optical microscopy, scanning electron microscopy （SEM）, and atomic force microscopy （AFM）, and the assembly mechanism was then analysed and discussed. BSA molecules were first linked by DNA molecule and formed linear chains. These chains then were parallelly linked through additional DNA hybridization. Finally, several BSA chains further assembled into fibers by layering lamellae in a parallel manner. This work perhaps will provide a guide to the immobilization of enzyme, which could be applied to increase its catalytic efficiency in biomedicine and nanotechnology.
