Assembled protein-based substances are emerging and promising classes of materials that provide unique properties for various applications in biotechnology and nanotechnolegy. Self-assembly is an effective way to immo...Assembled protein-based substances are emerging and promising classes of materials that provide unique properties for various applications in biotechnology and nanotechnolegy. Self-assembly is an effective way to immobilize protein. In this study, DNAs-conjugated bovine serum albumin （BSA） assembled into fibers via DNA hybridization is demonstrated. The morphology of fibers was observed by optical microscopy, scanning electron microscopy （SEM）, and atomic force microscopy （AFM）, and the assembly mechanism was then analysed and discussed. BSA molecules were first linked by DNA molecule and formed linear chains. These chains then were parallelly linked through additional DNA hybridization. Finally, several BSA chains further assembled into fibers by layering lamellae in a parallel manner. This work perhaps will provide a guide to the immobilization of enzyme, which could be applied to increase its catalytic efficiency in biomedicine and nanotechnology.展开更多
Objective: To evaluate p63 expression at mRNA transcripts and protein levels in lung squamous cell cancer (SCC), adenocarcinoma, large cell lung cancer (LCLC) and small cell lung cancer (SCLC) and their matched metast...Objective: To evaluate p63 expression at mRNA transcripts and protein levels in lung squamous cell cancer (SCC), adenocarcinoma, large cell lung cancer (LCLC) and small cell lung cancer (SCLC) and their matched metastatic tumors. The association between p63 expression and p63 locus at chromosomal 3q27 q29 was also investigated. Methods: p63 mRNA expression levels in a large series of lung cancers including SCC, adenocarcinoma, LCLC, SCLC and their matched metastatic tumors were analyzed by cDNA microarray technology. A tissue microarray from 150 primary lung cancer specimens was constructed and used for immunohistochemical detection of p63 protein expression. Chromosomal imbalances at the p63 locus in 70 primary lung cancers samples were studied by comparative genomic hybridization (CGH) technology. Results: mRNA levels were 10 fold in SCC compared to LCLC, SCLC, and adenocarcinoma. Interestingly, the mRNA expression of p63 in metastatic carcinomas was significantly higher than that in their matched primary tumors ( P <0 001). Immunohistochemistry demonstrated that p63 expression was 94.64% in SCC but only 1 79% in lung adenocarcinoma and 2 of 4 LCLC were positive staining. All the results in of SCLC were negative. There was a statistically significant difference for p63 positivity between pT1 tumors and those of higher stage ( P =0 035). The CGH results indicated that p63 locus at chromosomal 3q27 q29 was overrepresented in SCC. p63 immunopositivity correlated significantly with pronounced gains of the p63 locus at chromosomal 3q27 q29 (P=0.0001), indicating that strong expression of p63 in lung SCC correlated with increased gene amplification. Conclusion: p63 might play an important role not only in squamous differentiation of lung cancer but also in tumor development and progression.展开更多
Overview of the DNA damage response(DDR)in tumor cells.DDR is a highly coordinated signaling network that repairs DNA damage caused by intrinsic cellular processes and extrinsic insults,thereby preventing genome insta...Overview of the DNA damage response(DDR)in tumor cells.DDR is a highly coordinated signaling network that repairs DNA damage caused by intrinsic cellular processes and extrinsic insults,thereby preventing genome instability.Depending on the type of damage,distinct DNA damage repair and DNA damage tolerance(DDT)pathways are involved and coordinately regulated.展开更多
基金supported by the National Natural Science Foundation of China(NSFC, No. 21174029)the Industry Academia Cooperation Innovation Fund of Jiangsu Province(No.BY201412707)+1 种基金the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)the Fundamental Research Funds for the Central Universities (No. 2242016K41020)
文摘Assembled protein-based substances are emerging and promising classes of materials that provide unique properties for various applications in biotechnology and nanotechnolegy. Self-assembly is an effective way to immobilize protein. In this study, DNAs-conjugated bovine serum albumin （BSA） assembled into fibers via DNA hybridization is demonstrated. The morphology of fibers was observed by optical microscopy, scanning electron microscopy （SEM）, and atomic force microscopy （AFM）, and the assembly mechanism was then analysed and discussed. BSA molecules were first linked by DNA molecule and formed linear chains. These chains then were parallelly linked through additional DNA hybridization. Finally, several BSA chains further assembled into fibers by layering lamellae in a parallel manner. This work perhaps will provide a guide to the immobilization of enzyme, which could be applied to increase its catalytic efficiency in biomedicine and nanotechnology.
文摘Objective: To evaluate p63 expression at mRNA transcripts and protein levels in lung squamous cell cancer (SCC), adenocarcinoma, large cell lung cancer (LCLC) and small cell lung cancer (SCLC) and their matched metastatic tumors. The association between p63 expression and p63 locus at chromosomal 3q27 q29 was also investigated. Methods: p63 mRNA expression levels in a large series of lung cancers including SCC, adenocarcinoma, LCLC, SCLC and their matched metastatic tumors were analyzed by cDNA microarray technology. A tissue microarray from 150 primary lung cancer specimens was constructed and used for immunohistochemical detection of p63 protein expression. Chromosomal imbalances at the p63 locus in 70 primary lung cancers samples were studied by comparative genomic hybridization (CGH) technology. Results: mRNA levels were 10 fold in SCC compared to LCLC, SCLC, and adenocarcinoma. Interestingly, the mRNA expression of p63 in metastatic carcinomas was significantly higher than that in their matched primary tumors ( P <0 001). Immunohistochemistry demonstrated that p63 expression was 94.64% in SCC but only 1 79% in lung adenocarcinoma and 2 of 4 LCLC were positive staining. All the results in of SCLC were negative. There was a statistically significant difference for p63 positivity between pT1 tumors and those of higher stage ( P =0 035). The CGH results indicated that p63 locus at chromosomal 3q27 q29 was overrepresented in SCC. p63 immunopositivity correlated significantly with pronounced gains of the p63 locus at chromosomal 3q27 q29 (P=0.0001), indicating that strong expression of p63 in lung SCC correlated with increased gene amplification. Conclusion: p63 might play an important role not only in squamous differentiation of lung cancer but also in tumor development and progression.
基金the National Natural Science Foundation of China(Grant No.82330090 and Grant No.82341006 to C.G.)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDA0460403 to C.G.)the Natural Science Foundation of Shanxi Province(Grant No.202203021211155 to X.M.).
文摘Overview of the DNA damage response(DDR)in tumor cells.DDR is a highly coordinated signaling network that repairs DNA damage caused by intrinsic cellular processes and extrinsic insults,thereby preventing genome instability.Depending on the type of damage,distinct DNA damage repair and DNA damage tolerance(DDT)pathways are involved and coordinately regulated.
文摘目的探讨RUNX3、P16和DAPK基因启动子DNA甲基化与非小细胞肺癌(non-small cell lung cancer,NSCLC)化疗敏感性的关系。方法选取2023年2月至2025年2月于株洲市中心医院接受铂类化疗的初治晚期NSCLC患者116例为研究对象,根据化疗疗效将其分为敏感组(n=81)和耐受组(n=35)。比较两组患者的病灶组织标本RUNX3、P16、DAPK基因启动子DNA甲基化情况、基因转录水平绝对定量,采用受试者操作特征曲线(receiver operating characteristic curve,ROC曲线)评估RUNX3、P16、DAPK基因表达水平对铂类化疗耐药的预测价值。结果耐受组患者肺癌组织的RUNX3、P16和DAPK基因启动子DNA甲基化比例均显著高于敏感组(P<0.05)。耐受组患者肺癌组织的RUNX3、P16和DAPK基因转录水平均显著低于敏感组(P<0.05)。ROC曲线显示,肺癌组织RUNX3、P16、DAPK基因转录水平预测NSCLC患者化疗敏感的最佳截断值分别为2.93×10^(6)、5.22×10^(4)、6.420×10^(5),曲线下面积(area under the curve,AUC)分别为0.726、0.748、0.763。三者联合预测NSCLC患者化疗敏感的AUC为0.883,敏感度为81.82%、特异性为78.05%。结论RUNX3、P16、DAPK基因甲基化可能是晚期NSCLC患者铂类化疗耐药的原因之一,早期测定此类基因转录水平对预估化疗耐药、制定个体化治疗方案具有积极作用。
