Accurate evaluation of disease levels in wild rice germplasm and identification of disease resistance are critical for developing rice varieties resistant to blast disease.However,existing evaluation methods face limi...Accurate evaluation of disease levels in wild rice germplasm and identification of disease resistance are critical for developing rice varieties resistant to blast disease.However,existing evaluation methods face limitations that hinder progress in breeding.To address these challenges,we proposed an AI-powered method for evaluating blast disease levels and identifying resistance in wild rice.A lightweight segmentation model for diseased leaves and lesions was developed,incorporating an improved federated learning approach to enhance robustness and adaptability.Based on the segmentation results and resistance identification technical specifications,wild rice materials were evaluated into 10 disease levels(L0 to L9),further enabling disease-resistance identification through multiple replicates of the same materials.The method was successfully implemented on augmented reality glasses for real-time,first-person evaluation.Additionally,high-speed scanners and edge computing devices were integrated to enable continuous,precise,and dynamic evaluation.Experimental results demonstrate the outstanding performance of the proposed method,achieving effective segmentation of diseased leaves and lesions with only 0.22 M parameters and 5.3 G floating-point operations per second(FLOPs),with a mean average precision(mAP@0.5)of 96.3%.The accuracy of disease level evaluation and disease-resistance identification reached 99.7%,with a practical test accuracy of 99.0%,successfully identifying three highly resistant wild rice materials.This method provides strong technical support for efficiently identifying wild rice materials resistant to blast disease and advancing resistance breeding efforts.展开更多
Soil salinization is a globally prevalent abiotic environmental stress.The imbalance of ions caused by high concentrations of sodium chloride results in a 40%reduction in soybean yield.Soybean,as an important crop for...Soil salinization is a globally prevalent abiotic environmental stress.The imbalance of ions caused by high concentrations of sodium chloride results in a 40%reduction in soybean yield.Soybean,as an important crop for soil quality improvement,necessitates the identification of salt-tolerant varieties and germplasms to effectively utilize and enhance saline-alkali land.In this study,we assessed the salt tolerance of 435 soybean varieties and germplasms during the seedling stage.Among them,Qihuang34,You2104,Hongzhudou,Pamanheidou,and Osage exhibited grade 1 salt tolerance rates surpassing other tested materials.Furthermore,Hongzhudou and Qihuang34 demonstrated higher salt tolerance during germination and emergence stages based on their elevated rates of emergence,salt tolerance index,chlorophyll content,and shoot fresh weights.Overall findings provide valuable resources for molecular breeding efforts aimed at developing salt-tolerant soybean varieties suitable for cultivation in saline-alkali soils.展开更多
[Objectives]The paper was to analyze the yield characteristics of a new high-quality disease-resistant wheat variety Chuanmai 618.[Methods]The yield characteristics of Chuanmai 618 were analyzed using the AMMI model a...[Objectives]The paper was to analyze the yield characteristics of a new high-quality disease-resistant wheat variety Chuanmai 618.[Methods]The yield characteristics of Chuanmai 618 were analyzed using the AMMI model and GGE biplot based on data from a 2-year regional test and a 1-year production test.[Results]The analysis of the AMMI model for the 2-year regional test indicated that Chuanmai 618 had a moderate yield and good stability.During the production test,Chuanmai 618 had an average yield of 450.52 kg/666.7 m^(2),an effective spike of 235700 spike/666.7 m^(2),a 1000-seed weight of 47.93 g,and a kernel number per spike of 47.28.The AMMI analysis sequencing graph showed that the varieties were ranked in the following order:Zhongkemai 1816>Chuanmai 618>Shumai 1958>Chuanyu 42>Mianmai 367>Xikemai 5518.According to the GE analysis,Chuanmai 618 had comparative advantages.[Conclusions]The new wheat variety Chuanmai 618 is a high-quality disease-resistant variety with good yield and stability.展开更多
Germplasm resources are essential for the sustainable development of biodiversity and husbandry of local chickens, as well as for the breeding and industry of superior quality chickens. Unfortunately, many local and i...Germplasm resources are essential for the sustainable development of biodiversity and husbandry of local chickens, as well as for the breeding and industry of superior quality chickens. Unfortunately, many local and indigenous chicken breeds are at risk of declining numbers, emphasizing the need to conserve breed resources for endangered chickens. Primordial germ cells(PGCs) are crucial for preserving germplasm resources by inheriting genetic information from parents to offspring and ensuring stability of genetic material between germlines. In this study,PGCs were isolated from chicken embryos' gonads and cultured in FAcs medium without feeder cells. Over a period of approximately 40 d, the cells proliferated to a number of up to 10^(6), establishing various cell lines. Particularly, 18 PGC lines were created from Rugao Yellow chicken and Shouguang chicken, with an efficiency ranging from 39.1 to 45%. Furthermore, PGCs that had been cultured for 40 passages exhibited typical PGC characteristics, suchas glycogen staining reaction, and expression of pluripotency and reproductive markers. These results confirmthat PGCs maintain stem cell properties even after long-term in vitro culture. Additionally, PGCs cryopreserved for up to 120 d remained viable, maintained typical PGC morphologies, and possessed stable cell proliferation ability. Through intravascular injection into chicken embryos, green fluorescent protein(GFP)-PGCs were found in the recipient embryos' gonads and could develop into gametes to produce offspring, indicating that even after extended culture, PGCs retain their migratory and lineage-transmitting capabilities. This research offers valuable insights into the in vitro cultivation and preservation of PGCs of Chinese indigenous chickens. The findings of this study can be applied in transgenic chicken production and the preservation of genetic resources of indigenous chicken breeds.展开更多
Exploring the phenotypic trait variation and diversity of kiwifruit male plant resources can support selection,breeding,and genetic improvement,ultimately enhancing agricultural production.In this study,50 kiwifruit m...Exploring the phenotypic trait variation and diversity of kiwifruit male plant resources can support selection,breeding,and genetic improvement,ultimately enhancing agricultural production.In this study,50 kiwifruit male plants were collected from the resource nursery of Sichuan Provincial Natural Resources Bureau.The phenotypic variation of the germplasm was analyzed using 16 quantitative traits.The analysis involved coefficient of variation(CV),Shannon-Wiener index(H),principal component analysis,correlation analysis,cluster analysis,and comprehensive evaluation.The results showed that the variation range of 16 phenotypic traits in kiwifruit male germplasm resources was 1.55%to 83.71%,with an average coefficient of variation of 28.62%,and an H index of 1.265 to 2.941.The average CVs of diploid,tetraploid,and hexaploid were 22.62%,18.99%and 18.18%,respectively,and the average CV of diploid was the largest.Indicated that the male germplasm resources of kiwifruit showed significant phenotypic diversity,and the diploid showed higher diversity characteristics.Principal component analysis(PCA)revealed that the cumulative variance contribution rate of the first seven principal components was 76.66%,which effectively captured the information from 21 traits.Cluster analysis divided the 50 kiwifruit male germplasm resources into 4 clusters;each cluster exhibited distinct phenotypic characteristics.The analysis also determined the trait characteristics and breeding value of each cluster.The results of this study provide valuable information for genetic improvement,protection,and evaluation of kiwifruit male germplasm resources.展开更多
This paper investigated and analyzed the conservation and utilization of four local livestock breeds in Binzhou City:Wadi Sheep,Bohai Black Cattle,Wudi Donkey,and Lubei White Goat.Shortcomings in the protection and ut...This paper investigated and analyzed the conservation and utilization of four local livestock breeds in Binzhou City:Wadi Sheep,Bohai Black Cattle,Wudi Donkey,and Lubei White Goat.Shortcomings in the protection and utilization of local germplasm resources were pointed out,and strategies and recommendations were proposed to promote high-quality development of livestock and poultry genetic resources in Binzhou,including building a solid germplasm foundation,standardizing production,and driving innovation.This paper provides references for the conservation,development,and utilization of local genetic resources in Binzhou City.展开更多
The cashew tree is a typical tropical nut tree species in China, and the collection, identification and innovated utilization of excellent cashew germplasm resources are of great significance to increase the income of...The cashew tree is a typical tropical nut tree species in China, and the collection, identification and innovated utilization of excellent cashew germplasm resources are of great significance to increase the income of farmers and agricultural benefits in the tropical area of China. This study summarized the major advances on cashew germplasm resources in China, analyzed the major research progress and existing problems from the aspects of the collection and evaluation, resistances, genetic diversity of cashew germplasm resources, and put forward the work ideas to analyze the construction of core germplasm bank and the genetic stability of the major biological characteristics of cashew germplasm resources, which were of important significance for the improvement of the cultivar classification and resource evaluation system of cashew germplasm resources in China.展开更多
[Objective] This study aimed to carry out a preliminary analysis of genetic diversity of 47 JUNCAO germplasms. [Methods] Twenty-eight iPBS （Intel Primer Binding Site Amplification） primers were firstly used for PCR ...[Objective] This study aimed to carry out a preliminary analysis of genetic diversity of 47 JUNCAO germplasms. [Methods] Twenty-eight iPBS （Intel Primer Binding Site Amplification） primers were firstly used for PCR screening on a subset of four germplasms, of which 11 gave good amplification patterns and were then used for analyzing the DNA of 47 JUNCAO germplasms. [Result] A total of 208 polymorphic DNA fragments were scored among the 47 JUNCAO germplasms from the electrophoresis patterns of the 11 selected iPBS primers. By using the NTSYSpc 2.1 software combined with UPGMA clustering analysis method, the simple matching （SM） coefficient of similarity was calculated among all accessions and ranged from 0.58 to 0.99. The 47 JUNCAO germplasms were clustered into 10 categories at a genetic similarity coefficient of 0.67. All the 47 accessions were distinguished from each other. [Conclusion] Our results showed that iPBS markers could be effectively used for genetic diversity analysis of JUNCAO germplasms. This study provides a preliminary theoretical guidance and technical support for the efficient management and utilization of JUNCAO germplasm resources.展开更多
To estimate genetic variation in rhizome lotus （Nelumbo nucifera Gaertn. ssp. nucifera） germplasms in China, a total of 94 rhizome lotus germplasms collected from 18 provinces in China were assessed. The RAPD （rand...To estimate genetic variation in rhizome lotus （Nelumbo nucifera Gaertn. ssp. nucifera） germplasms in China, a total of 94 rhizome lotus germplasms collected from 18 provinces in China were assessed. The RAPD （randomly amplified polymorphic DNA） marker was employed. The selected 17 random primers detected 139 polymorphic alleles out of a total 207 （67.15%）. Nei＇s gene diversity statistics and region differentiation parameters indicated that all germplasms had a relatively high level of genetic diversity with ne = 1.3202, h = 0.1937, I= 0.2982 and the gene flow among all regions was Nrn = 5.5742. The UPGMA dendrogram clustered all 94 germplasms into two clusters： One contained eight commercial cultivars and major landraces, and the other included the wild and some special landraces from five regions, and the PCA analysis exhibited the similar result. Those germplasms from southwestern and eastern China had higher genetic diversity than those from the southern, northern and central China. Predominant proportion of genetic variation （95.61%） was found significant within rather than among （4.39%） regions, as revealed by AMOVA analysis. The data analysis also revealed that the genetic diversity of rhizome lotus germplasms among different regions is positively related to their geographic distances, though it is ambiguous to find the trend from the UPGMA dendrogram and the PCA analysis. A relatively high genetic diversity and gene flow resided in the root lotus germplasms; about 96% of the variation was found within region; accessions from southwest and eastern China have higher genetic diversity than those from the southern, northern and central China.展开更多
The lengths of mesocotyl in the seedlings of 84 lowland rice varieties and 12 upland rice varieties were measured following the treatments of daylight and darkness during germination. The elongation of mesocotyl in th...The lengths of mesocotyl in the seedlings of 84 lowland rice varieties and 12 upland rice varieties were measured following the treatments of daylight and darkness during germination. The elongation of mesocotyl in the varieties tested was inhibited under daylight condition, and the mesocotyl of all the varieties elongated variably under darkness condition. The elongated lengths of the mesocotyl in upland rice, ranging from 0.36 cm to 1,61 cm with an average of 0.81 cm, was obviously longer than those in lowland rice, ranging from 0.12 cm to 1.56 cm with an average of 0.42 cm. Among 14 rice varieties with over 1 cm of mesocotyl length, five belonged to upland rice, and nine to lowland rice. The possible utilization of the elongated-mesocotyl rice germplasm in varietal imorovement, direct-seeded plantina and seed ouritv testina were discussed.展开更多
Potato （Solanum tuberosum L.） is an important staple food and economic crop in many countries. China has led world potato production in recent years. To understand the genetic diversity of potato germplasms and to e...Potato （Solanum tuberosum L.） is an important staple food and economic crop in many countries. China has led world potato production in recent years. To understand the genetic diversity of potato germplasms and to enrich the current gene pool for potato improvement, we made a global collection consisted of 288 potato germplasms from eight countries and the International Potato Center （CIP）. Using SSR and AFLP techniques, we evaluated the genetic diversity and population structure of these 288 potato accessions. A total of 190 alleles on 20 SSR loci were detected and all of the SSR alleles were polymorphic among these potato germplasms with an average of 9.5 alleles per SSR locus ranging from 2 to 23. The effective number of alleles per locus （Ne＊）, Nei＇s genetic diversity （H＊）, and Shannon＇s information index （I＊） was from （0.1709＋0.3698） to （1.6166＋0.3414）, （0.076＋0.1388） to （0.3812＋0.1886）, and （0.1324＋0.1970） to （0.5347＋0.1440）, respec- tively, and the mean polymorphic information content （PIC） value was 0.7312. A total of 988 AFLP alleles were detected by 10 AFLP primer combinations with 983 polymorphic alleles, and 99.49% alleles was polymorphic with an average of 98.3 polymorphic alleles per primer combination ranging from 91 to 116. The values of Ne＊, H＊ and/＊ were from （1.5162＋0.311 ） to （1.6423＋0.3278）, （0.3114＋0.145） to （0.3675＋0.1121）, and （0.4761＋0.1792） to （0.547＋0.1322）, respectively, and the average PIC value was 0.9871. Bayesian analysis discriminated the accessions into seven subgroup and an admix group. The majority of accessions from CIP and China were assigned into SG1, SG5, SG6, SG7 and admix group. Accessions in SG3 were mainly from CIP and two small groups SG2 and SG4 were mainly from northeastern China. In general, the results obtained from Bayesian statistical analysis, cluster analysis and principal coordinate analysis consistently revealed the lack of geographical differentiation among country-wide collections, indicating germplasm introduction was common for the countries out of potato origin center. The poiymorphic markers and the differentiate genetic lineages found in this study provide useful information for potato improvement and conservation programs.展开更多
The genetic diversities of 110 pummelo germplasms and 12 of their relatives were analyzed by SSR and AFLP methods. Approximately 99.1% of the 335 SSR loci were polymorphic, and 9.85 alleles per SSR locus were identifi...The genetic diversities of 110 pummelo germplasms and 12 of their relatives were analyzed by SSR and AFLP methods. Approximately 99.1% of the 335 SSR loci were polymorphic, and 9.85 alleles per SSR locus were identified. The gene diversity values changed from 0.1939 to 0.9073, and 46 SSR polymorphic bands were scored. 72% of the 343 AFLP loci were polymorphic, and 82 polymorphic loci per AFLP were identified. Heterozygosity changed from 0.21863 to 0.28445, and 44 AFLP polymorphic bands were scored. The UPGMA result showed that 122 pummelo genotypes and their relatives could be divided into eight groups, and the pummelo genotypes composed mainly of Shatian pummelo varieties group, Wendan pummelo vareties group and a huge hybrid pummelo varieties group. The classification result was expected to widen the genetic background of pummelos using various target varieties.展开更多
Wild soybean （Glycine soja） is the wild relative species of cultivated soy- bean, which is of high protein content, strong tolerance and high propagation coeffi- cient. The diverse growing conditions of wild soybean...Wild soybean （Glycine soja） is the wild relative species of cultivated soy- bean, which is of high protein content, strong tolerance and high propagation coeffi- cient. The diverse growing conditions of wild soybean in China contribute to numer- ous phenotypes of wild soybean, which also reflect the evolution process through long-term natural and artificial selection. The research progress of wild soybean germpfasms was summarized in resource status and innovative utilization in order to provide information for research of wild soybean germplasms.展开更多
[Objective] This study aimed to explore the genetic relationship of purple tsai-tai germplasms using SSR molecular markers. [Method] SSR analysis of 45 purple tsai-tai samples was conducted with 65 pairs of primers se...[Objective] This study aimed to explore the genetic relationship of purple tsai-tai germplasms using SSR molecular markers. [Method] SSR analysis of 45 purple tsai-tai samples was conducted with 65 pairs of primers selected from cabbage primers, and the cluster analysis was carried out. [Result] A total of 23 pairs of SSR primers were screened; cluster analysis showed that the genetic relationship of purple tsai-tai germplasms had relatively significantly locality, and the 45 purple tsaitai samples can be divided into three groups of Sichuan, Hunan and Hubei; furthermore, the similarity coefficients of 45 purple tsai-tai samples were all greater than 0.5 （ranging from 0.547 0 to 0.910 7）, indicating that the closer the genetic relationship among purple tsai-tai samples is, the narrower the genetic basis will be. [Conclusion] This study provides a theoretical and technical basis for the identification, protection and breeding of the resources of local varieties of seaweed sprouts. This study provided theoretical and technical basis for the identification, protection, breeding and utilization of local purple tsai-tai resource.展开更多
Thousand Seed Weight （TSW） is one of the major yield components of rapeseed （Brassica napus L.）. Here reports an extra-large seed germplasm GM01 which was obtained through isolated microspore culture. Three-way cr...Thousand Seed Weight （TSW） is one of the major yield components of rapeseed （Brassica napus L.）. Here reports an extra-large seed germplasm GM01 which was obtained through isolated microspore culture. Three-way cross was made： H8--a Yunnan spring early-maturing rapeseed variety, ＂Legeney＂--a Canadian canola variety and ＂020010＂--a semi-winter late-maturing rapeseed variety. One hundred and forty eight doubled hyploid lines were obtained from the F~ plants of three-way cross through isolated mierospore culture. Among them, the TSW of GM01 amounted to 8.68 g and the TSW of 53 lines were above 5.0 g. The TSWs of GM01 were relatively stable among the multi-location field trials from 2007 to 2014 with variation being only 10%-15% among the locations and years. Compared with H8, GM01 had larger flowers, stigmas, siliques and seed diameters, but less branches, siliques per plant and seeds per silique.展开更多
Water is a major limiting factor for food production and many countries fail to produce sufficient food for their population due to severe water scarcity （Jury and Vaux, 2005）. Rice is the main staple food worldwide...Water is a major limiting factor for food production and many countries fail to produce sufficient food for their population due to severe water scarcity （Jury and Vaux, 2005）. Rice is the main staple food worldwide. More than 50% of rice in the world is rain-fed and drought causes severe reduction in rice grain yield in rain-fed environments （Venuprasad et al., 2007; Zhang, 2007; Sandhu et al., 2014）. Therefore, enhancing drought resistance （DR） of rice is important for food security. However, DR is a complex trait, which is controlled by a large number of loci with small effect and is also affected by different genetic background, genotype-by-environment interaction and other stresses such as heat （Hu and Xiong, 2014）.展开更多
[ Objective] This study aimed to identify and evaluate the resistance of bitter gourd germplasms to Meloidogyne incognita. [ Method] A total of 71 bit-ter gourd germplasms were artificially inoculated with M. i...[ Objective] This study aimed to identify and evaluate the resistance of bitter gourd germplasms to Meloidogyne incognita. [ Method] A total of 71 bit-ter gourd germplasms were artificially inoculated with M. incognita at seedling stage to investigate the effects of M. incognita on resistance indexes of bitter gourd seedlings. Cluster analysis and subordinate function analysis were performed to identify and evaluate the resistance of experimental materials. [ Result] The infec-tion of M. incognita increased the variation coefficients of resistance indexes. The resistance to M. incognita varied significantly among different bitter gourd germ- plasms. Based on gall index, 71 bitter gourd germplasms were divided into five types by cluster analysis, including resistant materials, moderately resistant materi-als ,moderately susceptible materials, susceptible materials and highly susceptible materials. The subordinate function analysis showed that total subordinate func-tion values of Y107, Y109, Y108, Y51, Y105, Y81, Y89, Y140 and Y94 exceeded 1.95 , indicating high resistance to M. incognita. [ Conclusion] This study could provide resistant materials for breeding root-knot nematode resistant varieties of bitter gourd.展开更多
The selection of cold tolerance identification indices for bitter gourd germplasms is an important basis for the breeding of cold-tolerant bitter gourd varieties. In this study, with six bitter gourd germplasms as exp...The selection of cold tolerance identification indices for bitter gourd germplasms is an important basis for the breeding of cold-tolerant bitter gourd varieties. In this study, with six bitter gourd germplasms as experimental materials, the cold injury index of bitter gourd was analyzed under low temperature treatment, the changes in physiological indices were revealed, and the main agronomic traits were measured. The results showed that the cold injury index of bitter gourd was in very significant negative correlation with SOD activity, POD activity and PRO content, in significant negative correlation with CAT activity, in very significant negative correlation with flowering node and commodity rate, but in significant positive correlation with female flower ratio. It was found that that cold injury index, SOD activity, POD activity, CAT activity and PRO content could serve as the cold tolerance identification indices for bitter gourd at seedling stage, and flowering node and commodity rate could be used as the auxiliary indices for field cold tolerance identification.展开更多
SRAP （Sequence-related amplified polymorphism）, a PCR-based molecular marker system was used to evaluate the genetic diversity of 48 JUCAO germplasms in this study. At first, 256 SRAP primer pairs were used to ampli...SRAP （Sequence-related amplified polymorphism）, a PCR-based molecular marker system was used to evaluate the genetic diversity of 48 JUCAO germplasms in this study. At first, 256 SRAP primer pairs were used to amplify four representative germplasms to screen the best primer pairs that could produce poly-morphic DNA fragments. As a result, a total of 284 polymorphic DNA fragments were scored among the 48 JUNCAO germplasms from the electrophoresis patterns generated with 18 selected SRAP primer pairs. By using NTSYS-pc 2.1 software combined with UPGMA cluster analysis, the genetic similarity （SM） coefficient be- tween the 48 accessions was calculated and ranged from 0.58 to 0.98. All the 48 accessions were distinguished from each other. The 48 JUNCAO germplasms were classified into four categories at a genetic similarity coefficient of 0.645. The results showed that the SRAP markers could be effectively used for analysis of genetic di- versify of JUNCAO germplasms. Additionally, the results also showed that there ex- ist abundant JUNCAO germptasm in the genus Arundo in China. This study provides a new technique for studying the genetic diversify of JUNCAO germplasms. The selected combinations of SRAP primers can be used for genetic analyses on a larger number of JUNCAO germplasms in the future. Moreover, the results provide theoretical guidance and technical support for the efficient management and utilization of JUNCAO germplasm resources.展开更多
基金supported by the National Key Research and Development Program of China(Grant Nos.2022YFF0711805,2022YFF0711801,and 2021YFF0704204)the Project of Sanya Yazhou Bay Science and Technology City,China(Grant No.SCKJ-JYRC-2023-45)+3 种基金the National Natural Science Foundation of China(Grant Nos.31971792 and 32160421)the Innovation Project of the Chinese Academy of Agricultural Sciences(CAAS)(Grant Nos.CAAS-ASTIP-2024-AII and ZDXM23011)the Special Fund of Chinese Central Government for Basic Scientific Research Operations in Commonweal Research Institutes(Grant No.JBYW-AII-2024-05)the Nanfan Special Project,CAAS,China(Grant No.YBXM2312).
文摘Accurate evaluation of disease levels in wild rice germplasm and identification of disease resistance are critical for developing rice varieties resistant to blast disease.However,existing evaluation methods face limitations that hinder progress in breeding.To address these challenges,we proposed an AI-powered method for evaluating blast disease levels and identifying resistance in wild rice.A lightweight segmentation model for diseased leaves and lesions was developed,incorporating an improved federated learning approach to enhance robustness and adaptability.Based on the segmentation results and resistance identification technical specifications,wild rice materials were evaluated into 10 disease levels(L0 to L9),further enabling disease-resistance identification through multiple replicates of the same materials.The method was successfully implemented on augmented reality glasses for real-time,first-person evaluation.Additionally,high-speed scanners and edge computing devices were integrated to enable continuous,precise,and dynamic evaluation.Experimental results demonstrate the outstanding performance of the proposed method,achieving effective segmentation of diseased leaves and lesions with only 0.22 M parameters and 5.3 G floating-point operations per second(FLOPs),with a mean average precision(mAP@0.5)of 96.3%.The accuracy of disease level evaluation and disease-resistance identification reached 99.7%,with a practical test accuracy of 99.0%,successfully identifying three highly resistant wild rice materials.This method provides strong technical support for efficiently identifying wild rice materials resistant to blast disease and advancing resistance breeding efforts.
基金supported by The National Natural Science Foundation of China(32171957)Scientific and Technological Innovation 2030,Design and Cultivation of New High-Yielding Salt-Alkali Tolerant Soybean Varieties(2023ZD0403602)Knowledge Innovation Program of Wuhan(2023020201010127).
文摘Soil salinization is a globally prevalent abiotic environmental stress.The imbalance of ions caused by high concentrations of sodium chloride results in a 40%reduction in soybean yield.Soybean,as an important crop for soil quality improvement,necessitates the identification of salt-tolerant varieties and germplasms to effectively utilize and enhance saline-alkali land.In this study,we assessed the salt tolerance of 435 soybean varieties and germplasms during the seedling stage.Among them,Qihuang34,You2104,Hongzhudou,Pamanheidou,and Osage exhibited grade 1 salt tolerance rates surpassing other tested materials.Furthermore,Hongzhudou and Qihuang34 demonstrated higher salt tolerance during germination and emergence stages based on their elevated rates of emergence,salt tolerance index,chlorophyll content,and shoot fresh weights.Overall findings provide valuable resources for molecular breeding efforts aimed at developing salt-tolerant soybean varieties suitable for cultivation in saline-alkali soils.
基金Sichuan"14 th Five-Year Plan"Wheat Breeding Tackling Project(2021YFYZ0002)Sichuan Provincial Financial Special Project(2021ZYGG-003)+2 种基金Sichuan Biological Breeding Major Science and Technology Program(2022ZDZX0014,2022ZDZX0016)Sichuan Science and Technology Program(2022JDRC0113,2022YFSY0015)Modern Discipline Construction Promotion Project of Sichuan Academy of Agricultural Sciences(2021XKJS005).
文摘[Objectives]The paper was to analyze the yield characteristics of a new high-quality disease-resistant wheat variety Chuanmai 618.[Methods]The yield characteristics of Chuanmai 618 were analyzed using the AMMI model and GGE biplot based on data from a 2-year regional test and a 1-year production test.[Results]The analysis of the AMMI model for the 2-year regional test indicated that Chuanmai 618 had a moderate yield and good stability.During the production test,Chuanmai 618 had an average yield of 450.52 kg/666.7 m^(2),an effective spike of 235700 spike/666.7 m^(2),a 1000-seed weight of 47.93 g,and a kernel number per spike of 47.28.The AMMI analysis sequencing graph showed that the varieties were ranked in the following order:Zhongkemai 1816>Chuanmai 618>Shumai 1958>Chuanyu 42>Mianmai 367>Xikemai 5518.According to the GE analysis,Chuanmai 618 had comparative advantages.[Conclusions]The new wheat variety Chuanmai 618 is a high-quality disease-resistant variety with good yield and stability.
基金supported by the National Key Research and Development Program of China (2021YFD1200301 and 2021YFD1200302)the Natural Science Foundation of Jiangsu Province, China (BK20210813)+1 种基金the National Natural Science Foundation of China (32102534)the Yangzhou International Science and Technology Cooperation Projects, China (YZ2021175)。
文摘Germplasm resources are essential for the sustainable development of biodiversity and husbandry of local chickens, as well as for the breeding and industry of superior quality chickens. Unfortunately, many local and indigenous chicken breeds are at risk of declining numbers, emphasizing the need to conserve breed resources for endangered chickens. Primordial germ cells(PGCs) are crucial for preserving germplasm resources by inheriting genetic information from parents to offspring and ensuring stability of genetic material between germlines. In this study,PGCs were isolated from chicken embryos' gonads and cultured in FAcs medium without feeder cells. Over a period of approximately 40 d, the cells proliferated to a number of up to 10^(6), establishing various cell lines. Particularly, 18 PGC lines were created from Rugao Yellow chicken and Shouguang chicken, with an efficiency ranging from 39.1 to 45%. Furthermore, PGCs that had been cultured for 40 passages exhibited typical PGC characteristics, suchas glycogen staining reaction, and expression of pluripotency and reproductive markers. These results confirmthat PGCs maintain stem cell properties even after long-term in vitro culture. Additionally, PGCs cryopreserved for up to 120 d remained viable, maintained typical PGC morphologies, and possessed stable cell proliferation ability. Through intravascular injection into chicken embryos, green fluorescent protein(GFP)-PGCs were found in the recipient embryos' gonads and could develop into gametes to produce offspring, indicating that even after extended culture, PGCs retain their migratory and lineage-transmitting capabilities. This research offers valuable insights into the in vitro cultivation and preservation of PGCs of Chinese indigenous chickens. The findings of this study can be applied in transgenic chicken production and the preservation of genetic resources of indigenous chicken breeds.
基金funded by Sichuan Science and Technology Program(2023YFH0006).
文摘Exploring the phenotypic trait variation and diversity of kiwifruit male plant resources can support selection,breeding,and genetic improvement,ultimately enhancing agricultural production.In this study,50 kiwifruit male plants were collected from the resource nursery of Sichuan Provincial Natural Resources Bureau.The phenotypic variation of the germplasm was analyzed using 16 quantitative traits.The analysis involved coefficient of variation(CV),Shannon-Wiener index(H),principal component analysis,correlation analysis,cluster analysis,and comprehensive evaluation.The results showed that the variation range of 16 phenotypic traits in kiwifruit male germplasm resources was 1.55%to 83.71%,with an average coefficient of variation of 28.62%,and an H index of 1.265 to 2.941.The average CVs of diploid,tetraploid,and hexaploid were 22.62%,18.99%and 18.18%,respectively,and the average CV of diploid was the largest.Indicated that the male germplasm resources of kiwifruit showed significant phenotypic diversity,and the diploid showed higher diversity characteristics.Principal component analysis(PCA)revealed that the cumulative variance contribution rate of the first seven principal components was 76.66%,which effectively captured the information from 21 traits.Cluster analysis divided the 50 kiwifruit male germplasm resources into 4 clusters;each cluster exhibited distinct phenotypic characteristics.The analysis also determined the trait characteristics and breeding value of each cluster.The results of this study provide valuable information for genetic improvement,protection,and evaluation of kiwifruit male germplasm resources.
基金Supported by Binzhou Social Sciences Planning Project in 2024(24-SKGH-051)Binzhou Comprehensive Experimental Station Project of Shandong Provincial Forage Industry Technology System(SDAIT-23-10).
文摘This paper investigated and analyzed the conservation and utilization of four local livestock breeds in Binzhou City:Wadi Sheep,Bohai Black Cattle,Wudi Donkey,and Lubei White Goat.Shortcomings in the protection and utilization of local germplasm resources were pointed out,and strategies and recommendations were proposed to promote high-quality development of livestock and poultry genetic resources in Binzhou,including building a solid germplasm foundation,standardizing production,and driving innovation.This paper provides references for the conservation,development,and utilization of local genetic resources in Binzhou City.
基金Supported by the National Nonprofit Institute Research Grant of CATAS-TCGRI(16300320150331630032014032+1 种基金PZSFYL-201613)the Ministry of Agriculture Tropical Crop Germplasm Resources Protection(16RZZY-101)~~
文摘The cashew tree is a typical tropical nut tree species in China, and the collection, identification and innovated utilization of excellent cashew germplasm resources are of great significance to increase the income of farmers and agricultural benefits in the tropical area of China. This study summarized the major advances on cashew germplasm resources in China, analyzed the major research progress and existing problems from the aspects of the collection and evaluation, resistances, genetic diversity of cashew germplasm resources, and put forward the work ideas to analyze the construction of core germplasm bank and the genetic stability of the major biological characteristics of cashew germplasm resources, which were of important significance for the improvement of the cultivar classification and resource evaluation system of cashew germplasm resources in China.
基金Supported by R&D Program of China National Engineering Research Center o JUNCAO Technology(JCGG14010)~~
文摘[Objective] This study aimed to carry out a preliminary analysis of genetic diversity of 47 JUNCAO germplasms. [Methods] Twenty-eight iPBS （Intel Primer Binding Site Amplification） primers were firstly used for PCR screening on a subset of four germplasms, of which 11 gave good amplification patterns and were then used for analyzing the DNA of 47 JUNCAO germplasms. [Result] A total of 208 polymorphic DNA fragments were scored among the 47 JUNCAO germplasms from the electrophoresis patterns of the 11 selected iPBS primers. By using the NTSYSpc 2.1 software combined with UPGMA clustering analysis method, the simple matching （SM） coefficient of similarity was calculated among all accessions and ranged from 0.58 to 0.99. The 47 JUNCAO germplasms were clustered into 10 categories at a genetic similarity coefficient of 0.67. All the 47 accessions were distinguished from each other. [Conclusion] Our results showed that iPBS markers could be effectively used for genetic diversity analysis of JUNCAO germplasms. This study provides a preliminary theoretical guidance and technical support for the efficient management and utilization of JUNCAO germplasm resources.
文摘To estimate genetic variation in rhizome lotus （Nelumbo nucifera Gaertn. ssp. nucifera） germplasms in China, a total of 94 rhizome lotus germplasms collected from 18 provinces in China were assessed. The RAPD （randomly amplified polymorphic DNA） marker was employed. The selected 17 random primers detected 139 polymorphic alleles out of a total 207 （67.15%）. Nei＇s gene diversity statistics and region differentiation parameters indicated that all germplasms had a relatively high level of genetic diversity with ne = 1.3202, h = 0.1937, I= 0.2982 and the gene flow among all regions was Nrn = 5.5742. The UPGMA dendrogram clustered all 94 germplasms into two clusters： One contained eight commercial cultivars and major landraces, and the other included the wild and some special landraces from five regions, and the PCA analysis exhibited the similar result. Those germplasms from southwestern and eastern China had higher genetic diversity than those from the southern, northern and central China. Predominant proportion of genetic variation （95.61%） was found significant within rather than among （4.39%） regions, as revealed by AMOVA analysis. The data analysis also revealed that the genetic diversity of rhizome lotus germplasms among different regions is positively related to their geographic distances, though it is ambiguous to find the trend from the UPGMA dendrogram and the PCA analysis. A relatively high genetic diversity and gene flow resided in the root lotus germplasms; about 96% of the variation was found within region; accessions from southwest and eastern China have higher genetic diversity than those from the southern, northern and central China.
基金the Special NationalPrograms for Pioneer Research(Projecn No.2002CCA04100)Zhejiang Provincial Key Programs for Scicnce and Technology(Project No.021102169)Natural Sciences Foundation of Zhejiang Province(Project No.301252).
文摘The lengths of mesocotyl in the seedlings of 84 lowland rice varieties and 12 upland rice varieties were measured following the treatments of daylight and darkness during germination. The elongation of mesocotyl in the varieties tested was inhibited under daylight condition, and the mesocotyl of all the varieties elongated variably under darkness condition. The elongated lengths of the mesocotyl in upland rice, ranging from 0.36 cm to 1,61 cm with an average of 0.81 cm, was obviously longer than those in lowland rice, ranging from 0.12 cm to 1.56 cm with an average of 0.42 cm. Among 14 rice varieties with over 1 cm of mesocotyl length, five belonged to upland rice, and nine to lowland rice. The possible utilization of the elongated-mesocotyl rice germplasm in varietal imorovement, direct-seeded plantina and seed ouritv testina were discussed.
基金supported by the earmarked fund for China Agriculture Research System(CARS-10)
文摘Potato （Solanum tuberosum L.） is an important staple food and economic crop in many countries. China has led world potato production in recent years. To understand the genetic diversity of potato germplasms and to enrich the current gene pool for potato improvement, we made a global collection consisted of 288 potato germplasms from eight countries and the International Potato Center （CIP）. Using SSR and AFLP techniques, we evaluated the genetic diversity and population structure of these 288 potato accessions. A total of 190 alleles on 20 SSR loci were detected and all of the SSR alleles were polymorphic among these potato germplasms with an average of 9.5 alleles per SSR locus ranging from 2 to 23. The effective number of alleles per locus （Ne＊）, Nei＇s genetic diversity （H＊）, and Shannon＇s information index （I＊） was from （0.1709＋0.3698） to （1.6166＋0.3414）, （0.076＋0.1388） to （0.3812＋0.1886）, and （0.1324＋0.1970） to （0.5347＋0.1440）, respec- tively, and the mean polymorphic information content （PIC） value was 0.7312. A total of 988 AFLP alleles were detected by 10 AFLP primer combinations with 983 polymorphic alleles, and 99.49% alleles was polymorphic with an average of 98.3 polymorphic alleles per primer combination ranging from 91 to 116. The values of Ne＊, H＊ and/＊ were from （1.5162＋0.311 ） to （1.6423＋0.3278）, （0.3114＋0.145） to （0.3675＋0.1121）, and （0.4761＋0.1792） to （0.547＋0.1322）, respectively, and the average PIC value was 0.9871. Bayesian analysis discriminated the accessions into seven subgroup and an admix group. The majority of accessions from CIP and China were assigned into SG1, SG5, SG6, SG7 and admix group. Accessions in SG3 were mainly from CIP and two small groups SG2 and SG4 were mainly from northeastern China. In general, the results obtained from Bayesian statistical analysis, cluster analysis and principal coordinate analysis consistently revealed the lack of geographical differentiation among country-wide collections, indicating germplasm introduction was common for the countries out of potato origin center. The poiymorphic markers and the differentiate genetic lineages found in this study provide useful information for potato improvement and conservation programs.
基金The project was financed by the National Natural Science Foundation of China(NSFC30070528)the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of Ministry of Education,P.R.China.We are thankful to the Citrus Research Institute,Chinese Academy of Agricultural Sciences+1 种基金Horticultural Institute,Jiangxi Academy of Agricultural Sciencesand Pummelo Germplasms Repository of Meixian County,Guangdong Province for providing part of plant materials.
文摘The genetic diversities of 110 pummelo germplasms and 12 of their relatives were analyzed by SSR and AFLP methods. Approximately 99.1% of the 335 SSR loci were polymorphic, and 9.85 alleles per SSR locus were identified. The gene diversity values changed from 0.1939 to 0.9073, and 46 SSR polymorphic bands were scored. 72% of the 343 AFLP loci were polymorphic, and 82 polymorphic loci per AFLP were identified. Heterozygosity changed from 0.21863 to 0.28445, and 44 AFLP polymorphic bands were scored. The UPGMA result showed that 122 pummelo genotypes and their relatives could be divided into eight groups, and the pummelo genotypes composed mainly of Shatian pummelo varieties group, Wendan pummelo vareties group and a huge hybrid pummelo varieties group. The classification result was expected to widen the genetic background of pummelos using various target varieties.
基金Supported by the Research and Demonstration of Green Transformation and New Agricultural Industry Mode in the Saline Alkali Land of the Yellow River Data,Key Research and Development (Industrial Key Technology) Project of Shandong Province(2016CYJS05A02)~~
文摘Wild soybean （Glycine soja） is the wild relative species of cultivated soy- bean, which is of high protein content, strong tolerance and high propagation coeffi- cient. The diverse growing conditions of wild soybean in China contribute to numer- ous phenotypes of wild soybean, which also reflect the evolution process through long-term natural and artificial selection. The research progress of wild soybean germpfasms was summarized in resource status and innovative utilization in order to provide information for research of wild soybean germplasms.
基金Supported by Hunan Provincial Natural Science Foundation of China(2008JJ3035)~~
文摘[Objective] This study aimed to explore the genetic relationship of purple tsai-tai germplasms using SSR molecular markers. [Method] SSR analysis of 45 purple tsai-tai samples was conducted with 65 pairs of primers selected from cabbage primers, and the cluster analysis was carried out. [Result] A total of 23 pairs of SSR primers were screened; cluster analysis showed that the genetic relationship of purple tsai-tai germplasms had relatively significantly locality, and the 45 purple tsaitai samples can be divided into three groups of Sichuan, Hunan and Hubei; furthermore, the similarity coefficients of 45 purple tsai-tai samples were all greater than 0.5 （ranging from 0.547 0 to 0.910 7）, indicating that the closer the genetic relationship among purple tsai-tai samples is, the narrower the genetic basis will be. [Conclusion] This study provides a theoretical and technical basis for the identification, protection and breeding of the resources of local varieties of seaweed sprouts. This study provided theoretical and technical basis for the identification, protection, breeding and utilization of local purple tsai-tai resource.
文摘Thousand Seed Weight （TSW） is one of the major yield components of rapeseed （Brassica napus L.）. Here reports an extra-large seed germplasm GM01 which was obtained through isolated microspore culture. Three-way cross was made： H8--a Yunnan spring early-maturing rapeseed variety, ＂Legeney＂--a Canadian canola variety and ＂020010＂--a semi-winter late-maturing rapeseed variety. One hundred and forty eight doubled hyploid lines were obtained from the F~ plants of three-way cross through isolated mierospore culture. Among them, the TSW of GM01 amounted to 8.68 g and the TSW of 53 lines were above 5.0 g. The TSWs of GM01 were relatively stable among the multi-location field trials from 2007 to 2014 with variation being only 10%-15% among the locations and years. Compared with H8, GM01 had larger flowers, stigmas, siliques and seed diameters, but less branches, siliques per plant and seeds per silique.
基金supported by grants from the National Program for Basic Research of China(No.2012CB114305)the National Program on High Technology Development(No. 2012AA10A303)the Oversea Graduate Program from Ministry of Education to K.Songyikhangsuthor
文摘Water is a major limiting factor for food production and many countries fail to produce sufficient food for their population due to severe water scarcity （Jury and Vaux, 2005）. Rice is the main staple food worldwide. More than 50% of rice in the world is rain-fed and drought causes severe reduction in rice grain yield in rain-fed environments （Venuprasad et al., 2007; Zhang, 2007; Sandhu et al., 2014）. Therefore, enhancing drought resistance （DR） of rice is important for food security. However, DR is a complex trait, which is controlled by a large number of loci with small effect and is also affected by different genetic background, genotype-by-environment interaction and other stresses such as heat （Hu and Xiong, 2014）.
基金Supported by National Nonprofit Institute Research Grant of CATAS-TCGRI(1630032014019,1630032015003)Key Research&Development Project of Hainan Province(ZDYF2016225)Key Technology Research and Demonstration Project of Farmland Improvement of Hainan Province(HNGDpz2015)
文摘[ Objective] This study aimed to identify and evaluate the resistance of bitter gourd germplasms to Meloidogyne incognita. [ Method] A total of 71 bit-ter gourd germplasms were artificially inoculated with M. incognita at seedling stage to investigate the effects of M. incognita on resistance indexes of bitter gourd seedlings. Cluster analysis and subordinate function analysis were performed to identify and evaluate the resistance of experimental materials. [ Result] The infec-tion of M. incognita increased the variation coefficients of resistance indexes. The resistance to M. incognita varied significantly among different bitter gourd germ- plasms. Based on gall index, 71 bitter gourd germplasms were divided into five types by cluster analysis, including resistant materials, moderately resistant materi-als ,moderately susceptible materials, susceptible materials and highly susceptible materials. The subordinate function analysis showed that total subordinate func-tion values of Y107, Y109, Y108, Y51, Y105, Y81, Y89, Y140 and Y94 exceeded 1.95 , indicating high resistance to M. incognita. [ Conclusion] This study could provide resistant materials for breeding root-knot nematode resistant varieties of bitter gourd.
基金Supported by National Nonprofit Institute Research Grant of CATAS-TCGRI(1630032015015,1630032017027,1630032016019)
文摘The selection of cold tolerance identification indices for bitter gourd germplasms is an important basis for the breeding of cold-tolerant bitter gourd varieties. In this study, with six bitter gourd germplasms as experimental materials, the cold injury index of bitter gourd was analyzed under low temperature treatment, the changes in physiological indices were revealed, and the main agronomic traits were measured. The results showed that the cold injury index of bitter gourd was in very significant negative correlation with SOD activity, POD activity and PRO content, in significant negative correlation with CAT activity, in very significant negative correlation with flowering node and commodity rate, but in significant positive correlation with female flower ratio. It was found that that cold injury index, SOD activity, POD activity, CAT activity and PRO content could serve as the cold tolerance identification indices for bitter gourd at seedling stage, and flowering node and commodity rate could be used as the auxiliary indices for field cold tolerance identification.
文摘SRAP （Sequence-related amplified polymorphism）, a PCR-based molecular marker system was used to evaluate the genetic diversity of 48 JUCAO germplasms in this study. At first, 256 SRAP primer pairs were used to amplify four representative germplasms to screen the best primer pairs that could produce poly-morphic DNA fragments. As a result, a total of 284 polymorphic DNA fragments were scored among the 48 JUNCAO germplasms from the electrophoresis patterns generated with 18 selected SRAP primer pairs. By using NTSYS-pc 2.1 software combined with UPGMA cluster analysis, the genetic similarity （SM） coefficient be- tween the 48 accessions was calculated and ranged from 0.58 to 0.98. All the 48 accessions were distinguished from each other. The 48 JUNCAO germplasms were classified into four categories at a genetic similarity coefficient of 0.645. The results showed that the SRAP markers could be effectively used for analysis of genetic di- versify of JUNCAO germplasms. Additionally, the results also showed that there ex- ist abundant JUNCAO germptasm in the genus Arundo in China. This study provides a new technique for studying the genetic diversify of JUNCAO germplasms. The selected combinations of SRAP primers can be used for genetic analyses on a larger number of JUNCAO germplasms in the future. Moreover, the results provide theoretical guidance and technical support for the efficient management and utilization of JUNCAO germplasm resources.
