New Voltage Differencing Differential Input Buffered Amplifier (VD-DIBA) based lossless grounded and floating inductance simulation circuits have been proposed. The proposed grounded simulated inductance circuit emplo...New Voltage Differencing Differential Input Buffered Amplifier (VD-DIBA) based lossless grounded and floating inductance simulation circuits have been proposed. The proposed grounded simulated inductance circuit employs a single VD-DIBA, one floating resistance and one grounded capacitor. The floating simulated inductance (FI) circuits employ two VD-DIBAs with two passive components (one floating resistance and one grounded capacitor). The circuit for grounded inductance does not require any realization conditions where as in case of floating inductance circuits, a single matching condition is needed. Simulation results demonstrating the applications of the new simulated inductors using CMOS VD-DIBAs have been included to confirm the workability of the new circuits.展开更多
Recently, voltage differencing-differential input buffered amplifiers (VD-DIBA)-based electronically controllable sinusoidal oscillator has been presented that it does not have the capability of complete independence ...Recently, voltage differencing-differential input buffered amplifiers (VD-DIBA)-based electronically controllable sinusoidal oscillator has been presented that it does not have the capability of complete independence of frequency of oscillation (FO) and condition of oscillation (CO) as well as electronic control of both CO and FO. In this article, a new fully-uncoupled electronically controllable sinusoidal oscillator using two VD-DIBAs, two grounded capacitors and two resistors has been proposed which offers important advantages such as 1) totally uncoupled and electronically controlled condition of oscillation (CO) and frequency of oscillation (FO);2) low active and passive sensitivities;and 3) a very good frequency stability factor. The effects of non-idealities of the VD-DIBAs on the proposed oscillator are also investigated. The validity of the proposed formulation has been confirmed by SPICE simulation with TSMC 0.18 μm process parameters.展开更多
Maize chlorotic mottle virus (MCMV) infects maize plants and causes significant losses in corn production worldwide. In this study, purified MCMV particles were used as the immunogen to produce monoclonal antibodies (...Maize chlorotic mottle virus (MCMV) infects maize plants and causes significant losses in corn production worldwide. In this study, purified MCMV particles were used as the immunogen to produce monoclonal antibodies (MAbs) and polyclonal antibodies (PAbs). Four murine MAbs (4B8, 8C11, 6F4, and 9G1) against MCMV were obtained through the hybridoma technology. The triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), dot-immunobinding assay (DIBA), and immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) using the MAb 4B8 were then developed for sensitive, specific, and rapid detection of MCMV in fields. MCMV could be detected in infected leaf crude extracts at dilutions of 1:327 680, 1:64000, and 1:3276800 (w/v, g/ml) by TAS-ELISA, DIBA, and IC-RT-PCR, respectively. One hundred and sixty-one maize field samples showing virus-like symptoms and sixty-nine symptomless maize field samples from ten different provinces of China were collected and screened for the presence of MCMV using the established serological methods. A phylogenetic tree was constructed based on the full length CP genes and Chinese MCMV isolates formed one branch with Thailand isolates. The detection results demonstrated that MCMV is one of most prevalent viruses infecting maize in the Yunnan and Sichuan provinces of China.展开更多
文摘New Voltage Differencing Differential Input Buffered Amplifier (VD-DIBA) based lossless grounded and floating inductance simulation circuits have been proposed. The proposed grounded simulated inductance circuit employs a single VD-DIBA, one floating resistance and one grounded capacitor. The floating simulated inductance (FI) circuits employ two VD-DIBAs with two passive components (one floating resistance and one grounded capacitor). The circuit for grounded inductance does not require any realization conditions where as in case of floating inductance circuits, a single matching condition is needed. Simulation results demonstrating the applications of the new simulated inductors using CMOS VD-DIBAs have been included to confirm the workability of the new circuits.
文摘Recently, voltage differencing-differential input buffered amplifiers (VD-DIBA)-based electronically controllable sinusoidal oscillator has been presented that it does not have the capability of complete independence of frequency of oscillation (FO) and condition of oscillation (CO) as well as electronic control of both CO and FO. In this article, a new fully-uncoupled electronically controllable sinusoidal oscillator using two VD-DIBAs, two grounded capacitors and two resistors has been proposed which offers important advantages such as 1) totally uncoupled and electronically controlled condition of oscillation (CO) and frequency of oscillation (FO);2) low active and passive sensitivities;and 3) a very good frequency stability factor. The effects of non-idealities of the VD-DIBAs on the proposed oscillator are also investigated. The validity of the proposed formulation has been confirmed by SPICE simulation with TSMC 0.18 μm process parameters.
基金supported by the National Natural Science Foundation of China (No. 31272015)the Ministry of Education of China (No. 313052)the Zhejiang Provincial Natural Science Foundation of China (No. Z3090039)
文摘Maize chlorotic mottle virus (MCMV) infects maize plants and causes significant losses in corn production worldwide. In this study, purified MCMV particles were used as the immunogen to produce monoclonal antibodies (MAbs) and polyclonal antibodies (PAbs). Four murine MAbs (4B8, 8C11, 6F4, and 9G1) against MCMV were obtained through the hybridoma technology. The triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), dot-immunobinding assay (DIBA), and immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) using the MAb 4B8 were then developed for sensitive, specific, and rapid detection of MCMV in fields. MCMV could be detected in infected leaf crude extracts at dilutions of 1:327 680, 1:64000, and 1:3276800 (w/v, g/ml) by TAS-ELISA, DIBA, and IC-RT-PCR, respectively. One hundred and sixty-one maize field samples showing virus-like symptoms and sixty-nine symptomless maize field samples from ten different provinces of China were collected and screened for the presence of MCMV using the established serological methods. A phylogenetic tree was constructed based on the full length CP genes and Chinese MCMV isolates formed one branch with Thailand isolates. The detection results demonstrated that MCMV is one of most prevalent viruses infecting maize in the Yunnan and Sichuan provinces of China.
