The microbial degradation of aromatic organic pollutants is incomplete due to their metabolic characteristics,which can easily produce certain highly toxic intermediates.Therefore,this article designs a dual template ...The microbial degradation of aromatic organic pollutants is incomplete due to their metabolic characteristics,which can easily produce certain highly toxic intermediates.Therefore,this article designs a dual template molec-ularly imprinted sensor(DTMIP/Fe-Mn@C)for iron manganese metal nanomaterials,prepared Fe-Mn@C com-posite materials by a one pot method were coated on the surface of glassy carbon electrodes and covered with molecularly imprinted membranes through electropolymerization and elution methods,achieving real-time de-tection of specific intermediate products 2-methylbutyric acid(2-MBA)and 3-methylbutyric acid(3-MBA)de-graded by azo dyes.In order to determine the detection sensitivity and intensity range of the sensor,optimization experiments were conducted on various parameters that affect the detection performance,such as the type of func-tional monomer and its composition ratio with the template molecule,detection time window,environmental pH value,etc.Finally,o-Phenylenediamine was determined as the functional monomer,with a molar ratio of 1:1:6 to the template molecules 2-MBA and 3-MBA.Electrochemical testing was conducted in a neutral environment with an incubation time of 5 min and pH=7.The results indicate that the sensor has a relatively wide detection range,high sensitivity,obvious recognition features,and excellent stability for 2-MBA and 3-MBA.This new dual template molecularly imprinted sensor can quickly and accurately determine the safety of highly toxic interme-diates in the degradation process of aromatic organic pollutants,providing a theoretical basis and application potential for trace detection and real-time monitoring.展开更多
The increasing production and release of synthetic organic chemicals,including pharmaceuticals,into our envi-ronment has allowed these substances to accumulate in our surface water systems.Current purification technol...The increasing production and release of synthetic organic chemicals,including pharmaceuticals,into our envi-ronment has allowed these substances to accumulate in our surface water systems.Current purification technolo-gies have been unable to eliminate these pollutants,resulting in their ongoing release into aquatic ecosystems.This study focuses on cloperastine(CPS),a cough suppressant and antihistamine medication.The environmental impact of CPS usage has become a concern,mainly due to its increased detection during the COVID-19 pandemic.CPS has been found in wastewater treatment facilities,effluents from senior living residences,river waters,and sewage sludge.However,the photosensitivity of CPS and its photodegradation profile remain largely unknown.This study investigates the photodegradation process of CPS under simulated tertiary treatment conditions using UV photolysis,a method commonly applied in some wastewater treatment plants.Several transformation prod-ucts were identified,evaluating their kinetic profiles using chemometric approaches(i.e.,curve fitting and the hard-soft multivariate curve resolution-alternating least squares(HS-MCR-ALS)algorithm)and calculating the reaction quantum yield.As a result,three different transformation products have been detected and correctly identified.In addition,a comprehensive description of the kinetic pathway involved in the photodegradation process of the CPS drug has been provided,including observed kinetic rate constants.展开更多
In this work,we constructed a three-dimensional electrochemical system(3D-ECO),which included the cathode and anode electrode plates,as well as the screening of three-dimensional particle electrodes and parameter opti...In this work,we constructed a three-dimensional electrochemical system(3D-ECO),which included the cathode and anode electrode plates,as well as the screening of three-dimensional particle electrodes and parameter opti-mization,for the degradation of landfill leachate(LL)containing elevated levels of tetracycline(TC),and explored its mechanism of action.Firstly,titanium-based ruthenium-iridium(Ti/RuO_(2)-IrO_(2)),titanium-based ruthenium-iridium-platinum(Ti/Pt-RuO_(2)-IrO_(2)),and titanium-based tin-antimony(Ti/SnO_(2)-Sb_(2)O_(3))were employed as an-odes in the electrocatalytic oxidation system,with titanium and stainless steel plates serving as cathodes,to construct the optimal two-dimensional electrocatalytic oxidation system(2D-ECO)through cross-comparison ex-periments.Subsequently,using granular activated carbon(GAC),coconut shell biochar(CBC),walnut shell carbon(WBC),and bamboo charcoal(BBC)as particle electrodes,a 3D-ECO system was developed.The influence of var-ious operational parameters on treating TC-containing LL was investigated.The optimal operating parameters obtained from the study was:pH=5,current density of 30 mA/cm^(2),particle dosage of 7 g/L,particle size ranging from 1.70 to 2.00 mm,and electrode spacing of 4 cm.Under these conditions,the COD removal rate of 3D-ECO within three hours was 90.25%,the TC removal rate was 72.41%,and the NH_(3)-N removal rate was 39.52%.The removal of TC followed a pseudo-first-order kinetic model.Additionally,degradation mechanisms were elucidated through electron paramagnetic resonance(EPR)spectrometer and Tert-Butanol(TBA)quenching experiments,indicating that the degradation primarily occurred through a non-radical(1O_(2))pathway.This re-search offers a comprehensive analysis of the simultaneous breakdown of intricate LL matrices and TC,enhancing our comprehension of the degradation processes and underlying mechanisms.展开更多
Poly(butylene adipate-terephthalate)(PBAT),as one of the most common and promising biodegradable plastics,has been widely used in agriculture,packaging,and other industries due to its strong biodegradability propertie...Poly(butylene adipate-terephthalate)(PBAT),as one of the most common and promising biodegradable plastics,has been widely used in agriculture,packaging,and other industries due to its strong biodegradability properties.It is well known that PBAT suffers a series of natural weathering,mechanical wear,hydrolysis,photochemical transformation,and other abiotic degradation processes before being biodegraded.Therefore,it is particularly important to understand the role of abiotic degradation in the life cycle of PBAT.Since the abiotic degradation of PBAT has not been systematically summarized,this review aims to summarize the mechanisms and main factors of the three major abiotic degradation pathways(hydrolysis,photochemical transformation,and thermochemical degradation)of PBAT.It was found that all of them preferentially destroy the chemical bonds with higher energy(especially C-O and C=O)of PBAT,which eventually leads to the shortening of the polymer chain and then leads to reduction in molecular weight.The main factors affecting these abiotic degradations are closely related to the energy or PBAT structure.These findings provide important theoretical and practical guidance for identifying effective methods for PBAT waste management and proposing advanced schemes to regulate the degradation rate of PBAT.展开更多
Exploration of stable metal single-site supported porous graphitic carbon nitride(PCN)nanostructures and the development of maximum atom utilization for enhanced photocatalytic oxidation of antibiotics remains a chall...Exploration of stable metal single-site supported porous graphitic carbon nitride(PCN)nanostructures and the development of maximum atom utilization for enhanced photocatalytic oxidation of antibiotics remains a challenge in current research.This work proposed a one-step thermal copolymerization to obtain Cu(Ⅰ)doping porous carbon nitride(CUCN)through a spontaneously reducing atmosphere by urea in a covered crucible.The obtained CUCN had crumpled ultrathin nanosheets and mesoporous structures,which possessed higher specific surface areas than PCN.From X-ray absorption near edge structure(XANES)and Fourier transform extended X-ray absorption fine structure(FT-EXAFS)spectra analysis,the Cu doping existed in the oxidation state of Cu(Ⅰ)as single atoms anchored on the 2D layers of CN through two N neighbors,thereby facilitating efficient pathways for the transfer of photoexcited charge carriers.Furthermore,the photoluminescence(PL)spectra,electrochemical impedance spectra(EIS)and transient photocurrent response test proved the improved separation and transfer of photoexcited charge carriers for Cu(Ⅰ)introduction.Consequently,the photocatalytic activity of CUCN was much better than that of PCN for antibiotics norfloxacin(NOR),with 4.7-fold higher degradation reaction rate constants.From species-trapping experiments and density function theory(DFT)calculations,the Cu single atoms in Cu-N_(2)served as catalytic sites that could accelerate charge transfer and facilitate the adsorption of molecular oxygen to produce active species.The stable Cu(Ⅰ)embedded in the layer structure led to the excellent recycling test and remained stable after four runs of degradation and even thermal regenerated treatment.The degradation paths of NOR by CUCN under visible light were also demonstrated.Our work sheds light on a sustainable and practical approach for achieving stable metal single-atom doping and enhancing photocatalytic degradation of aqueous pollutants.展开更多
Dissolved organic matter(DOM)is very important in aquatic environments,yet it is challenging to characterize DOM as a highly complex mixture of thousands of molecules,and the knowledge of the effects of different degr...Dissolved organic matter(DOM)is very important in aquatic environments,yet it is challenging to characterize DOM as a highly complex mixture of thousands of molecules,and the knowledge of the effects of different degradation processes on different molecules remains limited.This study examined the distribution and degradation of DOM in a large subtropical river using optical techniques and Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR-MS).At the molecular level,DOM was mainly composed of CHO and lignin-like compounds,which was related to the dominance of forestland in the watershed and resulted in a low biological lability index(MLBL).The modified aromaticity index(AI_(mod)),unsaturation degree(DBE),and humic content(HIX)decreased while MLBL,H/C,absorption spectral slope(S_(275–295)),and biological index(BIX)increased in the estuarine zone due to the increasing autochthonous contribution.Photo-and microbial degradation resulted in a similar decrease in the bulk dissolved organic carbon,while they showed opposite effects on the DOM composition.Photo-degradation removed all fluorescent components and decreased molecular weight,HIX,AI_(mod),DBE,%CHO,%lignin-like,%tannin-like,and%condensed aromatic-like compounds.In contrast,bio-degradation preferentially consumed lipid-like,protein-like,and carbohydrate-like compounds,with increases in%ligninlike,%tannin-like,%condensed aromatic-like compounds,and humic-like fluorescent components.Overall,the application of ultra-high resolutionmass spectrometry provided valuable insights into the composition and behavior of DOM at themolecular level and revealed the contrasting effects of photo-and microbial degradation on different compounds.These results have implications for better understanding the composition and transformation of aquatic DOM.展开更多
The selection of carbon sources and the biosynthesis of polyhydroxybutyrate(PHB)by the Azotobacter vinelandii N-15 strain using renewable raw materials were investigated.Among the tested substrates(starch,sucrose,mola...The selection of carbon sources and the biosynthesis of polyhydroxybutyrate(PHB)by the Azotobacter vinelandii N-15 strain using renewable raw materials were investigated.Among the tested substrates(starch,sucrose,molasses,bran),molasses as the carbon source yielded the highest PHB production.The maximum polymer yield(26%of dry biomass)was achieved at a molasses concentration of 40 g/L.PHB formation was confirmed via thinlayer chromatography,gas chromatography and Fourier transform infrared spectroscopy.Composite films based on PHB,polylactic acid(PLA),and their blends were fabricated using the solvent casting.The biodegradation of these films was studied with bacteria isolated from plastic-contaminated soil.These bacteria utilized the biopolymers as their sole carbon source,with the biodegradation process lasting three months.Structural and chemical changes in the films were analyzed using FTIR spectroscopy,differential scanning calorimetry,and thermogravimetry.Among the microorganisms used to study the biodegradation of PHB,PLA,and their blends,Streptomyces sp.K2 and Streptomyces sp.K4 exhibited the highest biodegradation efficiency.PHB-containing films demonstrated significant advantages over other biodegradable polymers,as they degrade under aerobic conditions via enzymatic hydrolysis using microbial depolymerases.展开更多
This paper presents a multi-scale experimental investigation of the weathering degradation of red mudstone.Natural rocks were extracted from the surface ground to 120 m,inwhich three sets of samples were selected to c...This paper presents a multi-scale experimental investigation of the weathering degradation of red mudstone.Natural rocks were extracted from the surface ground to 120 m,inwhich three sets of samples were selected to consider the different initial rock fabrics.The long-term relative humidity(RH)cycles under two amplitudes were imposed on red mudstone to simulate the weathering process.After RH cycles,a series of uniaxial compression tests,Brazilian splitting tests and bender-extender element tests were carried out to examine the reduction in strength and stiffness.The objective of this study is to develop an extended stress-volume framework characterizing the degradation of natural red mudstone both at microscale and macroscale.Accompanied by the irreversible swelling of the rock specimen is the progressive degradation of strength,stiffness and Poisson's ratio.A unified exponential degradation model in terms of the irreversible volumetric strain was thus proposed to capture such a degradation pattern.The effect of the initial rock fabric was evident.The highest degradation rate and potential were identified in slightly weathered specimens.Significant slaking of aggregates and crack propagation were confirmed by scanning electron microscope(SEM)micrographs,which were considered as the main consequence of structure damage leading to degradation of mechanical properties.The structure damage during RH cycles denoted the hysteresis nature in the response to the cycling hydraulic reaction,in turn causing the increase in volumetric strain.Thus,the stress-volume relation rather than the suction relation was found in more reasonable agreement with the experimental results.展开更多
Wide bandgap semiconductors are typically activated under ultraviolet(UV)light irradiation for volatile organic compounds(VOCs)degradation.However,our previous study discovered that certain VOCs can interact with some...Wide bandgap semiconductors are typically activated under ultraviolet(UV)light irradiation for volatile organic compounds(VOCs)degradation.However,our previous study discovered that certain VOCs can interact with some wide bandgap semiconductors,formatting an intermediate bandgap between the VOCs and the conduction band of wide bandgap semiconductor,thus inducing visible light activation of the system,and photo-generated electrons are excited by visible light and transferred from the VOCs to the conduction band of semiconductor.In this work,BaTiO_(3),traditionally is not active under visible light irradiation,however showed degradation rates of 100%and 20%for styrene and toluene under visible light,respectively.Density functional theory(DFT)calculations indicate that the adsorption of styrene or toluene on the BaTiO_(3)surface reduces its bandgap from 2.93 eV to 1.36 eV and 2.26 eV,respectively.The intermediate bandgap in this system is primarily formed by the valence band of BaTiO_(3)and the VOCs,and indicating that photo-generated electrons directly transfer from BaTiO_(3)to the VOCs under visible light,inducing degradation reactions of VOCs,i.e.,this work discovered a new transfer pathway of photo-electrons direct from the valence band of BaTiO_(3)to VOCs,while photo-electrons are from VOCs to the conductive band of wide-bandgap semiconductors in our previous work.展开更多
Nicotine,also known as nicotinic norephedrine,is one of the main alkaloids present in tobacco plants.In recent years,due to the increase in tobacco production and smoking population,the environmental and health issues...Nicotine,also known as nicotinic norephedrine,is one of the main alkaloids present in tobacco plants.In recent years,due to the increase in tobacco production and smoking population,the environmental and health issues caused by nicotine have become increasingly severe.Traditional methods have proven ineffective in efficiently degrading residual nicotine.To address this issue,scientists both domestically and internationally have turned to biodegradation methods to tackle the environmental and health problems caused by residual nicotine.In this study,an enrichment method was used to screen bacteria with nicotine-degrading capabilities from the soil of tobacco planting sites at the Tobacco Research Institute of Heilongjiang in Bin County,Harbin City.Through phenotypic observations and 16S rDNA identification,a bacterial strain identified as Pseudomonas hunanensis MGJ-2 was isolated,capable of utilizing nicotine as a carbon and nitrogen source for growth.High-performance liquid chromatography(HPLC)-1 analysis revealed that within 25 h,strain MGJ-2 could degrade nicotine 500 mg·L^(-1) with an efficiency exceeding 99.9%.Strain MGJ-2 was applied to tobacco,and after 15 days of incubation and fermentation,it degraded 10.57%of nicotine in tobacco.Overall,the discovery of strain MGJ-2 enriched the resources of nicotine-degrading strains.Its remarkable biodegradation performance held immense potential for future biodegradation of nicotine in tobacco.展开更多
BACKGROUND Gastric cancer(GC)is a prevalent malignancy with a substantial health burden and high mortality rate,despite advances in prevention,early detection,and treatment.Compared with the global average,Asia,notabl...BACKGROUND Gastric cancer(GC)is a prevalent malignancy with a substantial health burden and high mortality rate,despite advances in prevention,early detection,and treatment.Compared with the global average,Asia,notably China,reports disproportionately high GC incidences.The disease often progresses asymptoma-tically in the early stages,leading to delayed diagnosis and compromised out-comes.Thus,it is crucial to identify early diagnostic biomarkers and enhance treatment strategies to improve patient outcomes and reduce mortality.METHODS Retrospectively analyzed the clinical data of 148 patients with GC treated at the Civil Aviation Shanghai Hospital between December 2022 and December 2023.The associations of coagulation indices-partial thromboplastin time(APTT),prothrombin time(PT),thrombin time(TT),fibrinogen,fibrinogen degradation products(FDP),fasting blood glucose,and D-dimer(D-D)with TNM stage and distant metastasis were examined.RESULTS Prolongation of APTT,PT,and TT was significantly correlated with the GC TNM stage.Hence,abnormal coagulation system activation was closely related to disease progression.Elevated FDP and D-D were significantly associated with distant metastasis in GC(P<0.05),suggesting that increased fibrinolytic activity contributes to increased metastatic risk.CONCLUSION Our Results reveal coagulation indices,FDPs as GC biomarkers,reflecting abnormal coagulation/fibrinolysis,aiding disease progression,metastasis prediction,and helping clinicians assess thrombotic risk for early intervention and personalized treatment plans.展开更多
With the approaching of large-scale retirement of power lithium-ion batteries(LIBs),their urgent handling is required for environmental protection and resource reutilization.However,at present,substantial spent power ...With the approaching of large-scale retirement of power lithium-ion batteries(LIBs),their urgent handling is required for environmental protection and resource reutilization.However,at present,substantial spent power batteries,especially for those high recovery value cathode materials,have not been greenly,sustainably,and efficiently recycled.Compared to the traditional recovery method for cathode materials with high energy consumption and severe secondary pollution,the direct repair regeneration,as a new type of short-process and efficient treatment methods,has attracted widespread attention.However,it still faces challenges in homogenization repair,electrochemical performance decline,and scaling-up production.To promote the direct regeneration technology development of failed NCM materials,herein we deeply discuss the failure mechanism of nickel-cobalt-manganese(NCM)ternary cathode materials,including element loss,Li/Ni mixing,phase transformation,structural defects,oxygen release,and surface degradation and reconstruction.Based on this,the detailed analysis and summary of the direct regeneration method embracing solid-phase sintering,eutectic salt assistance,solvothermal synthesis,sol-gel process,spray drying,and redox mediation are provided.Further,the upcycling strategy for regeneration materials,such as single-crystallization and high-nickelization,structural regulation,ion doping,and surface engineering,are discussed in deep.Finally,the challenges faced by the direct regeneration and corresponding countermeasures are pointed out.Undoubtedly,this review provides valuable guidance for the efficient and high-value recovery of failed cathode materials.展开更多
Pure Mg boasting a relatively small corrosion rate is a potential biodegradable metal material for implants.However,its degradation behavior in the complex physiological environment is still a lack of understanding.In...Pure Mg boasting a relatively small corrosion rate is a potential biodegradable metal material for implants.However,its degradation behavior in the complex physiological environment is still a lack of understanding.In this work,we investigated the effect of corrosion product film layers on the degradation behavior of pure Mg in physiological environments.Pure Mg shows a faster corrosion rate in simulated body fluid(SBF)compared to NaCl solution.Hydrogen evolution experiments indicate that the degradation rate of pure Mg in SBF decreases rapidly within the first 12 h but stabilizes afterward.The rapid deposition of low-solubility calcium phosphate on the pure Mg in SBF provides protection to the substrate,resulting in a gradual decrease in the degradation rates.Consequently,the corrosion product film of pure Mg formed in SBF exhibits a layered structure,with the upper layer consisting of dense Ca_(3)(PO_(4))_(2)/Mg_(3)(PO_(4))_(2) and the lower layer consisting of Mg(OH)_(2)/MgO.Electrochemical impedance spectroscopy(EIS)shows that the resistance of the corrosion product film increases over time,indicating gradual strengthening of the corrosion resistance.The 4-week degradation results in the femoral marrow cavity of mice are consistent with the result in SBF in vitro.展开更多
In this paper,the property degradation micromechanism of Al-5.10Cu-0.65 Mg-0.8Mn(wt%)alloy induced by 0.5 wt%Fe minor addition was revealed by atomic-scale scanning transmission electron microscopy and energy-dispersi...In this paper,the property degradation micromechanism of Al-5.10Cu-0.65 Mg-0.8Mn(wt%)alloy induced by 0.5 wt%Fe minor addition was revealed by atomic-scale scanning transmission electron microscopy and energy-dispersive X-ray spectroscopy coupled with first-principles calculations.The results show that the Fe minor addition to the Al-Cu-Mg-Mn alloy leads to a slight reduction of grain size and the formation of coarse Al7Cu2Fe constituent particles.Fe tends to segregate into the T-phase dispersoids,θ'-,and S-phase precipitates by preferentially occupying Cu or Mn sites in these phase structures.The apparent Fe segregation contributes to an increase in stiffness of the T-phase and S-phase but decreased stiffness of theθ'phase.Formation of the coarse Al7Cu2Fe constituent particles and decreased stiffness of main precipitatesθ'containing Fe result in the degraded strength of the Al-Cu-Mg-Mn-Fe alloy.Further study reveals that corrosion resistance degradation of the Al-Cu-Mg-Mn-Fe alloy is associated with the increased width of precipitation free zones and consecutive grain boundary precipitates.The obtained results have significant implications for the usage of recycled Al alloys and the potential design strategies of high-performance alloys containing Fe.展开更多
Solar-driven Fenton-like reactions are promising strategies for degrading pharmaceutical wastewater to address environmental challenges and antibiotic pollution.However,its efficacy is limited by suboptimal light abso...Solar-driven Fenton-like reactions are promising strategies for degrading pharmaceutical wastewater to address environmental challenges and antibiotic pollution.However,its efficacy is limited by suboptimal light absorption efficiency,rapid charge recombination,and inadequate interfacial charge transfer.In this study,an inorganic/organic S-scheme photo-Fenton system of pseudobrookite/carbon nitride(FTOCN)was synthesized via a hydrothermally coupled calcination process for the effective purification of tetracycline antibiotics under visible-light irradiation.The optimized FTOCN-2 heterostructure exhibits a significantly enhanced TC degradation capacity of 90%within 60 min.The rate constant of FTOCN-2 is 1.6 and 5.2 times greater than those of FTO and CN,respectively.Furthermore,FTOCN exhibits high antibacterial efficacy,highlighting its potential application in the purification of natural water.Measurements via a range of analytical techniques,including Kelvin probe force microscopy,density functional theory calculations,in situ X-ray photoelectron spectroscopy,and femtosecond transient absorption spectroscopy,corroborate the S-scheme mechanism.This study provides a novel perspective for the development of photo-Fenton systems with S-scheme heterojunctions for water purification.展开更多
Nicosulfuron(NSR),a sulfonylurea herbicide,readily infiltrates water bodies,potentially compromising aquatic ecosystems and human health.In this study,bacteria consortium YM2 was isolated and cultivated from pesticide...Nicosulfuron(NSR),a sulfonylurea herbicide,readily infiltrates water bodies,potentially compromising aquatic ecosystems and human health.In this study,bacteria consortium YM2 was isolated and cultivated from pesticide plant active sludge for NSR wastewater bioremediation.Response surface methodology analysis demonstrated that under optimal cultivation conditions(9.41 g L^(-1)maltodextrin,21.37 g L^(-1)yeast extract,and 12.45 g L^(-1)NaCl),the YM2 bacteria consortium achieved 97.49%NSR degradation within 4 d.Optimal degradation parameters were established at 30℃,pH 6.0,1%inoculum,and 20 mg L^(-1)initial NSR concentration.The degradation system demonstrated resistance to heavy metal ions including Cd^(2+),Pb^(2+),Ni^(2+),and Zn^(2+),with degradation primarily occurring through bacterial extracellular enzymes(92.17%).During the degradation process,reactive oxygen species,oxidative stress,cell membrane permeability,cell surface hydrophobicity,and apoptosis rate exhibited initial increases followed by decreases.Additionally,bioflm formation-related genes luxS,waaE,spo0A,and wza showed temporal and concentration-dependent expression patterns.NSR concentrations in wastewater and soil were reduced to 1.92 and 2.72 mg L^(-1),respectively.In a simulated wastewater treatment unit with a 12-h hydraulic retention time,YM2 achieved 84.55%NSR degradation after 10 d.These fndings provide a theoretical foundation for microbial remediation of NSR contamination.展开更多
Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation,epigenetic factor,and post-translational modification.Previously,we reported that MaERF11 cooperat...Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation,epigenetic factor,and post-translational modification.Previously,we reported that MaERF11 cooperated with MaHDA1 to precisely regulate the transcription of ripening-associated genes via histone deacetylation.However,whether MaERF11 is subjected to post-translational modification during banana ripening is largely unknown.In this study,we found that MaERF11 targeted a subset of starch degradation-related genes using the DNA affinity purification sequence(DAP-Seq)approach.Electrophoretic mobility shift assay(EMSA)and dual-luciferase reporter assay(DLR)demonstrated that MaERF11 could specifically bind and repress the expression of the starch degradation-related genes MaAMY3,MaBAM2 and MaGWD1.Further analyses of yeast two-hybrid(Y2H),bimolecular fluorescence complementation(BiFC)and Luciferase complementation imaging(LCI)assays indicated that MaERF11 interacted with the ubiquitin E3 ligase MaRFA1,and this interaction weakened the MaERF11-mediated transcriptional repression capacity.Collectively,our results suggest an additional regulatory layer in which MaERF11 regulates banana fruit ripening and expands the regulatory network in fruit ripening at the post-translational modification level.展开更多
At present,carbon capture and storage(CCS)is the only mature and commercialized technology capable of effectively and economically reducing greenhouse gas emissions to achieve a significant and immedi-ate impact on th...At present,carbon capture and storage(CCS)is the only mature and commercialized technology capable of effectively and economically reducing greenhouse gas emissions to achieve a significant and immedi-ate impact on the CO_(2) level on Earth.Notably,long-term geological storage of captured CO_(2) has emerged as a primary storage method,given its minimal impact on surface ecological environments and high level of safety.The integrity of CO_(2) storage wellbores can be compromised by the corrosion of steel casings and degradation of cement in supercritical CO_(2) storage environments,potentially leading to the leakage of stored CO_(2) from the sites.This critical review endeavors to establish a knowledge foundation for the cor-rosion and materials degradation associated with geological CO_(2) storage through an in-depth examina-tion and analysis of the environments,operation,and the state-of-the-art progress in research pertaining to the topic.This article discusses the physical and chemical properties of CO_(2) in its supercrit-ical phase during injection and storage.It then introduces the principle of geological CO_(2) storage,consid-erations in the construction of storage systems,and the unique geo-bio-chemical environment involving aqueous media and microbial communities in CO_(2) storage.After a comprehensive analysis of existing knowledge on corrosion in CO_(2) storage,including corrosion mechanisms,parametric effects,and corro-sion rate measurements,this review identifies technical gaps and puts forward potential avenues for fur-ther research in steel corrosion within geological CO_(2) storage systems.展开更多
High-concentration single-atom doping remains a formidable challenge due to the propensity for single atoms to form clusters or aggregate at elevated concentrations.Herein,high-concentration (10.8 wt%) Zn singleatom-d...High-concentration single-atom doping remains a formidable challenge due to the propensity for single atoms to form clusters or aggregate at elevated concentrations.Herein,high-concentration (10.8 wt%) Zn singleatom-doped porous tubular g-C_(3)N_(4)(ZCN) was successfully obtained via a template-free,one-step calcination method,exhibiting excellent photocatalytic performance.The confinement of the pore walls suppresses the Zn atom'smigration and aggregation,enhancing the Zn single-atom stability.ZCN exhibited excellent photodegradation performance against tetracycline with outstanding stability.Moreover,ZCN displayed remarkable sterilization performance,achieving a 100%inactivation of Staphylococcus aureus within 90 min of visible-light exposure.Density functional theory calculations demonstrated that the Zn single-atom sites act as pivotal photocatalytic active sites,with the presence of Zn single atoms notably augmenting charge separation efficiency.This work provides a novel approach for managing photocatalytic efficiency through enlarging single-atom doping,offering an avenue for pollutant photodegradation and sterilization.展开更多
Although solar steam generation strategy is efficient in desalinating seawater,it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic pollutants.Herei...Although solar steam generation strategy is efficient in desalinating seawater,it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic pollutants.Herein,dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co_(2)(OH)_(2)CO_(3)nanorod top layer and a bacterial cellulose/Co_(2)(OH)_(2)CO_(3)nanorod(BCH)bottom layer.Crucially,the hydrogen bonding networks inside the membrane can be tuned by the rich surface–OH groups of the bacterial cellulose and Co_(2)(OH)_(2)CO_(3)as well as the ions and radicals in situ generated during the catalysis process.Moreover,both SO_(4)^(2−)and HSO_(5)−can regulate the solvation structure of Na^(+)and be adsorbed more preferentially on the evaporation surface than Cl^(−),thus hindering the de-solvation of the solvated Na^(+)and subsequent nucleation/growth of NaCl.Furthermore,the heat generated by the solar-thermal energy conversion can accelerate the reaction kinetics and enhance the catalytic degradation efficiency.This work provides a flow-bed water purification system with an asymmetric solar-thermal and catalytic membrane for synergistic solar thermal desalination of seawater/brine and catalytic degradation of organic pollutants.展开更多
基金supported by the Bingtuan Industrial Technology Research Institute,Bingtuan New materials Research Institute innovation platform project,Research initiation project of Shihezi University(No.RCZK202330)the Science and Technology Program-Regional Innovation Guidance Program(No.2023ZD080)Tianchi Talent Project(No.CZ002735).
文摘The microbial degradation of aromatic organic pollutants is incomplete due to their metabolic characteristics,which can easily produce certain highly toxic intermediates.Therefore,this article designs a dual template molec-ularly imprinted sensor(DTMIP/Fe-Mn@C)for iron manganese metal nanomaterials,prepared Fe-Mn@C com-posite materials by a one pot method were coated on the surface of glassy carbon electrodes and covered with molecularly imprinted membranes through electropolymerization and elution methods,achieving real-time de-tection of specific intermediate products 2-methylbutyric acid(2-MBA)and 3-methylbutyric acid(3-MBA)de-graded by azo dyes.In order to determine the detection sensitivity and intensity range of the sensor,optimization experiments were conducted on various parameters that affect the detection performance,such as the type of func-tional monomer and its composition ratio with the template molecule,detection time window,environmental pH value,etc.Finally,o-Phenylenediamine was determined as the functional monomer,with a molar ratio of 1:1:6 to the template molecules 2-MBA and 3-MBA.Electrochemical testing was conducted in a neutral environment with an incubation time of 5 min and pH=7.The results indicate that the sensor has a relatively wide detection range,high sensitivity,obvious recognition features,and excellent stability for 2-MBA and 3-MBA.This new dual template molecularly imprinted sensor can quickly and accurately determine the safety of highly toxic interme-diates in the degradation process of aromatic organic pollutants,providing a theoretical basis and application potential for trace detection and real-time monitoring.
基金supported by the grants PID2020-113371RA-C22 and TED2021-130845A-C32,funded by MCIN/AEI/10.13039/501100011033.M.Marín-García,R.González-OlmosC.Gómez-Canela are members of the GESPA group(Grup d’Enginyeria i Simulacióde Processos Ambientals)at IQS-URL,which has been acknowledged as a Consolidated Research Group by the Government of Catalonia(No.2021-SGR-00321)+1 种基金In addition,M.Marín-García has been awarded a public grant for the Investigo Programme,aimed at hiring young job seekers to undertake research and innovation projects under the Recovery,Transformation,and Resilience Plan(PRTR),European Union Next Generation,for the year 2022,through the Government of Catalonia and the Spanish Ministry for Work and Social Economy(No.100045ID16)Ana Belén Cuenca for her support and expertise,which helped to confirm the proposed reaction mechanism involved in the UV photolysis of cloperastine.
文摘The increasing production and release of synthetic organic chemicals,including pharmaceuticals,into our envi-ronment has allowed these substances to accumulate in our surface water systems.Current purification technolo-gies have been unable to eliminate these pollutants,resulting in their ongoing release into aquatic ecosystems.This study focuses on cloperastine(CPS),a cough suppressant and antihistamine medication.The environmental impact of CPS usage has become a concern,mainly due to its increased detection during the COVID-19 pandemic.CPS has been found in wastewater treatment facilities,effluents from senior living residences,river waters,and sewage sludge.However,the photosensitivity of CPS and its photodegradation profile remain largely unknown.This study investigates the photodegradation process of CPS under simulated tertiary treatment conditions using UV photolysis,a method commonly applied in some wastewater treatment plants.Several transformation prod-ucts were identified,evaluating their kinetic profiles using chemometric approaches(i.e.,curve fitting and the hard-soft multivariate curve resolution-alternating least squares(HS-MCR-ALS)algorithm)and calculating the reaction quantum yield.As a result,three different transformation products have been detected and correctly identified.In addition,a comprehensive description of the kinetic pathway involved in the photodegradation process of the CPS drug has been provided,including observed kinetic rate constants.
基金supported by the National Natural Science Foundation of China(Nos.42477406 and 51878617)the Horizontal Scientific Research Project(No.KYY-HX-20220803)the Engineering Research Center of Ministry of Education for Renewable Energy Infrastructure Construction Technology.
文摘In this work,we constructed a three-dimensional electrochemical system(3D-ECO),which included the cathode and anode electrode plates,as well as the screening of three-dimensional particle electrodes and parameter opti-mization,for the degradation of landfill leachate(LL)containing elevated levels of tetracycline(TC),and explored its mechanism of action.Firstly,titanium-based ruthenium-iridium(Ti/RuO_(2)-IrO_(2)),titanium-based ruthenium-iridium-platinum(Ti/Pt-RuO_(2)-IrO_(2)),and titanium-based tin-antimony(Ti/SnO_(2)-Sb_(2)O_(3))were employed as an-odes in the electrocatalytic oxidation system,with titanium and stainless steel plates serving as cathodes,to construct the optimal two-dimensional electrocatalytic oxidation system(2D-ECO)through cross-comparison ex-periments.Subsequently,using granular activated carbon(GAC),coconut shell biochar(CBC),walnut shell carbon(WBC),and bamboo charcoal(BBC)as particle electrodes,a 3D-ECO system was developed.The influence of var-ious operational parameters on treating TC-containing LL was investigated.The optimal operating parameters obtained from the study was:pH=5,current density of 30 mA/cm^(2),particle dosage of 7 g/L,particle size ranging from 1.70 to 2.00 mm,and electrode spacing of 4 cm.Under these conditions,the COD removal rate of 3D-ECO within three hours was 90.25%,the TC removal rate was 72.41%,and the NH_(3)-N removal rate was 39.52%.The removal of TC followed a pseudo-first-order kinetic model.Additionally,degradation mechanisms were elucidated through electron paramagnetic resonance(EPR)spectrometer and Tert-Butanol(TBA)quenching experiments,indicating that the degradation primarily occurred through a non-radical(1O_(2))pathway.This re-search offers a comprehensive analysis of the simultaneous breakdown of intricate LL matrices and TC,enhancing our comprehension of the degradation processes and underlying mechanisms.
基金supported by the National Key R&D Program of China(No.2022YFC3901800)the National Natural Science Foundation of China(No.22176041)Guangzhou Science and Technology Planning Project(No.2023A04J0918)。
文摘Poly(butylene adipate-terephthalate)(PBAT),as one of the most common and promising biodegradable plastics,has been widely used in agriculture,packaging,and other industries due to its strong biodegradability properties.It is well known that PBAT suffers a series of natural weathering,mechanical wear,hydrolysis,photochemical transformation,and other abiotic degradation processes before being biodegraded.Therefore,it is particularly important to understand the role of abiotic degradation in the life cycle of PBAT.Since the abiotic degradation of PBAT has not been systematically summarized,this review aims to summarize the mechanisms and main factors of the three major abiotic degradation pathways(hydrolysis,photochemical transformation,and thermochemical degradation)of PBAT.It was found that all of them preferentially destroy the chemical bonds with higher energy(especially C-O and C=O)of PBAT,which eventually leads to the shortening of the polymer chain and then leads to reduction in molecular weight.The main factors affecting these abiotic degradations are closely related to the energy or PBAT structure.These findings provide important theoretical and practical guidance for identifying effective methods for PBAT waste management and proposing advanced schemes to regulate the degradation rate of PBAT.
基金supported by the National Natural Science Foundation of China(Nos.52070103 and 22102102)Zhejiang Provincial Natural Science Foundation of China(Nos.LY21E090004 and LQ22B050004)+1 种基金Ningbo Public Welfare Science and Technology Program(No.2021S025)Ningbo Youth Leading Talent Project(No.2024QL038).
文摘Exploration of stable metal single-site supported porous graphitic carbon nitride(PCN)nanostructures and the development of maximum atom utilization for enhanced photocatalytic oxidation of antibiotics remains a challenge in current research.This work proposed a one-step thermal copolymerization to obtain Cu(Ⅰ)doping porous carbon nitride(CUCN)through a spontaneously reducing atmosphere by urea in a covered crucible.The obtained CUCN had crumpled ultrathin nanosheets and mesoporous structures,which possessed higher specific surface areas than PCN.From X-ray absorption near edge structure(XANES)and Fourier transform extended X-ray absorption fine structure(FT-EXAFS)spectra analysis,the Cu doping existed in the oxidation state of Cu(Ⅰ)as single atoms anchored on the 2D layers of CN through two N neighbors,thereby facilitating efficient pathways for the transfer of photoexcited charge carriers.Furthermore,the photoluminescence(PL)spectra,electrochemical impedance spectra(EIS)and transient photocurrent response test proved the improved separation and transfer of photoexcited charge carriers for Cu(Ⅰ)introduction.Consequently,the photocatalytic activity of CUCN was much better than that of PCN for antibiotics norfloxacin(NOR),with 4.7-fold higher degradation reaction rate constants.From species-trapping experiments and density function theory(DFT)calculations,the Cu single atoms in Cu-N_(2)served as catalytic sites that could accelerate charge transfer and facilitate the adsorption of molecular oxygen to produce active species.The stable Cu(Ⅰ)embedded in the layer structure led to the excellent recycling test and remained stable after four runs of degradation and even thermal regenerated treatment.The degradation paths of NOR by CUCN under visible light were also demonstrated.Our work sheds light on a sustainable and practical approach for achieving stable metal single-atom doping and enhancing photocatalytic degradation of aqueous pollutants.
基金supported by Fujian Provincial Natural Science Foundation of China(Nos.2023J01456 and 2021J01626)the National Natural Science Foundation of China(No.41976042)the Oceanic Interdisciplinary Program of Shanghai Jiao Tong University(Nos.SL2022ZD207 and SL2023MS019).
文摘Dissolved organic matter(DOM)is very important in aquatic environments,yet it is challenging to characterize DOM as a highly complex mixture of thousands of molecules,and the knowledge of the effects of different degradation processes on different molecules remains limited.This study examined the distribution and degradation of DOM in a large subtropical river using optical techniques and Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR-MS).At the molecular level,DOM was mainly composed of CHO and lignin-like compounds,which was related to the dominance of forestland in the watershed and resulted in a low biological lability index(MLBL).The modified aromaticity index(AI_(mod)),unsaturation degree(DBE),and humic content(HIX)decreased while MLBL,H/C,absorption spectral slope(S_(275–295)),and biological index(BIX)increased in the estuarine zone due to the increasing autochthonous contribution.Photo-and microbial degradation resulted in a similar decrease in the bulk dissolved organic carbon,while they showed opposite effects on the DOM composition.Photo-degradation removed all fluorescent components and decreased molecular weight,HIX,AI_(mod),DBE,%CHO,%lignin-like,%tannin-like,and%condensed aromatic-like compounds.In contrast,bio-degradation preferentially consumed lipid-like,protein-like,and carbohydrate-like compounds,with increases in%ligninlike,%tannin-like,%condensed aromatic-like compounds,and humic-like fluorescent components.Overall,the application of ultra-high resolutionmass spectrometry provided valuable insights into the composition and behavior of DOM at themolecular level and revealed the contrasting effects of photo-and microbial degradation on different compounds.These results have implications for better understanding the composition and transformation of aquatic DOM.
基金financial support of this paper by the Ministry of Education and Science of Ukraine under grant(Biotherm/0124U000789).
文摘The selection of carbon sources and the biosynthesis of polyhydroxybutyrate(PHB)by the Azotobacter vinelandii N-15 strain using renewable raw materials were investigated.Among the tested substrates(starch,sucrose,molasses,bran),molasses as the carbon source yielded the highest PHB production.The maximum polymer yield(26%of dry biomass)was achieved at a molasses concentration of 40 g/L.PHB formation was confirmed via thinlayer chromatography,gas chromatography and Fourier transform infrared spectroscopy.Composite films based on PHB,polylactic acid(PLA),and their blends were fabricated using the solvent casting.The biodegradation of these films was studied with bacteria isolated from plastic-contaminated soil.These bacteria utilized the biopolymers as their sole carbon source,with the biodegradation process lasting three months.Structural and chemical changes in the films were analyzed using FTIR spectroscopy,differential scanning calorimetry,and thermogravimetry.Among the microorganisms used to study the biodegradation of PHB,PLA,and their blends,Streptomyces sp.K2 and Streptomyces sp.K4 exhibited the highest biodegradation efficiency.PHB-containing films demonstrated significant advantages over other biodegradable polymers,as they degrade under aerobic conditions via enzymatic hydrolysis using microbial depolymerases.
基金The financial support from Project(Grant Nos.52278432,and 52168066)of National Natural Science Foundation of China and Project(Grant No.K2023G033)of the Science and Technology Research and Development Plan of China National Railway Group Co.,Ltd.were greatly appreciated.
文摘This paper presents a multi-scale experimental investigation of the weathering degradation of red mudstone.Natural rocks were extracted from the surface ground to 120 m,inwhich three sets of samples were selected to consider the different initial rock fabrics.The long-term relative humidity(RH)cycles under two amplitudes were imposed on red mudstone to simulate the weathering process.After RH cycles,a series of uniaxial compression tests,Brazilian splitting tests and bender-extender element tests were carried out to examine the reduction in strength and stiffness.The objective of this study is to develop an extended stress-volume framework characterizing the degradation of natural red mudstone both at microscale and macroscale.Accompanied by the irreversible swelling of the rock specimen is the progressive degradation of strength,stiffness and Poisson's ratio.A unified exponential degradation model in terms of the irreversible volumetric strain was thus proposed to capture such a degradation pattern.The effect of the initial rock fabric was evident.The highest degradation rate and potential were identified in slightly weathered specimens.Significant slaking of aggregates and crack propagation were confirmed by scanning electron microscope(SEM)micrographs,which were considered as the main consequence of structure damage leading to degradation of mechanical properties.The structure damage during RH cycles denoted the hysteresis nature in the response to the cycling hydraulic reaction,in turn causing the increase in volumetric strain.Thus,the stress-volume relation rather than the suction relation was found in more reasonable agreement with the experimental results.
基金financially supported by the National Natural Science Foundation of China(No.22176041,T2421005)National Key R&D Program of China(No.2022YFC3901800)+1 种基金the Fundamental Research Funds for the Central Universities(No.2243200011)Guangzhou Science and Technology Planning Project(No.2023A04J0918).
文摘Wide bandgap semiconductors are typically activated under ultraviolet(UV)light irradiation for volatile organic compounds(VOCs)degradation.However,our previous study discovered that certain VOCs can interact with some wide bandgap semiconductors,formatting an intermediate bandgap between the VOCs and the conduction band of wide bandgap semiconductor,thus inducing visible light activation of the system,and photo-generated electrons are excited by visible light and transferred from the VOCs to the conduction band of semiconductor.In this work,BaTiO_(3),traditionally is not active under visible light irradiation,however showed degradation rates of 100%and 20%for styrene and toluene under visible light,respectively.Density functional theory(DFT)calculations indicate that the adsorption of styrene or toluene on the BaTiO_(3)surface reduces its bandgap from 2.93 eV to 1.36 eV and 2.26 eV,respectively.The intermediate bandgap in this system is primarily formed by the valence band of BaTiO_(3)and the VOCs,and indicating that photo-generated electrons directly transfer from BaTiO_(3)to the VOCs under visible light,inducing degradation reactions of VOCs,i.e.,this work discovered a new transfer pathway of photo-electrons direct from the valence band of BaTiO_(3)to VOCs,while photo-electrons are from VOCs to the conductive band of wide-bandgap semiconductors in our previous work.
基金Supported by the Research on Biofermentation Technology of Domestic Cigar Tobacco(202115010534-JS-178)(2021)。
文摘Nicotine,also known as nicotinic norephedrine,is one of the main alkaloids present in tobacco plants.In recent years,due to the increase in tobacco production and smoking population,the environmental and health issues caused by nicotine have become increasingly severe.Traditional methods have proven ineffective in efficiently degrading residual nicotine.To address this issue,scientists both domestically and internationally have turned to biodegradation methods to tackle the environmental and health problems caused by residual nicotine.In this study,an enrichment method was used to screen bacteria with nicotine-degrading capabilities from the soil of tobacco planting sites at the Tobacco Research Institute of Heilongjiang in Bin County,Harbin City.Through phenotypic observations and 16S rDNA identification,a bacterial strain identified as Pseudomonas hunanensis MGJ-2 was isolated,capable of utilizing nicotine as a carbon and nitrogen source for growth.High-performance liquid chromatography(HPLC)-1 analysis revealed that within 25 h,strain MGJ-2 could degrade nicotine 500 mg·L^(-1) with an efficiency exceeding 99.9%.Strain MGJ-2 was applied to tobacco,and after 15 days of incubation and fermentation,it degraded 10.57%of nicotine in tobacco.Overall,the discovery of strain MGJ-2 enriched the resources of nicotine-degrading strains.Its remarkable biodegradation performance held immense potential for future biodegradation of nicotine in tobacco.
文摘BACKGROUND Gastric cancer(GC)is a prevalent malignancy with a substantial health burden and high mortality rate,despite advances in prevention,early detection,and treatment.Compared with the global average,Asia,notably China,reports disproportionately high GC incidences.The disease often progresses asymptoma-tically in the early stages,leading to delayed diagnosis and compromised out-comes.Thus,it is crucial to identify early diagnostic biomarkers and enhance treatment strategies to improve patient outcomes and reduce mortality.METHODS Retrospectively analyzed the clinical data of 148 patients with GC treated at the Civil Aviation Shanghai Hospital between December 2022 and December 2023.The associations of coagulation indices-partial thromboplastin time(APTT),prothrombin time(PT),thrombin time(TT),fibrinogen,fibrinogen degradation products(FDP),fasting blood glucose,and D-dimer(D-D)with TNM stage and distant metastasis were examined.RESULTS Prolongation of APTT,PT,and TT was significantly correlated with the GC TNM stage.Hence,abnormal coagulation system activation was closely related to disease progression.Elevated FDP and D-D were significantly associated with distant metastasis in GC(P<0.05),suggesting that increased fibrinolytic activity contributes to increased metastatic risk.CONCLUSION Our Results reveal coagulation indices,FDPs as GC biomarkers,reflecting abnormal coagulation/fibrinolysis,aiding disease progression,metastasis prediction,and helping clinicians assess thrombotic risk for early intervention and personalized treatment plans.
基金financially supported by the National Key Research and Development Program of China(2023YFB3809300)。
文摘With the approaching of large-scale retirement of power lithium-ion batteries(LIBs),their urgent handling is required for environmental protection and resource reutilization.However,at present,substantial spent power batteries,especially for those high recovery value cathode materials,have not been greenly,sustainably,and efficiently recycled.Compared to the traditional recovery method for cathode materials with high energy consumption and severe secondary pollution,the direct repair regeneration,as a new type of short-process and efficient treatment methods,has attracted widespread attention.However,it still faces challenges in homogenization repair,electrochemical performance decline,and scaling-up production.To promote the direct regeneration technology development of failed NCM materials,herein we deeply discuss the failure mechanism of nickel-cobalt-manganese(NCM)ternary cathode materials,including element loss,Li/Ni mixing,phase transformation,structural defects,oxygen release,and surface degradation and reconstruction.Based on this,the detailed analysis and summary of the direct regeneration method embracing solid-phase sintering,eutectic salt assistance,solvothermal synthesis,sol-gel process,spray drying,and redox mediation are provided.Further,the upcycling strategy for regeneration materials,such as single-crystallization and high-nickelization,structural regulation,ion doping,and surface engineering,are discussed in deep.Finally,the challenges faced by the direct regeneration and corresponding countermeasures are pointed out.Undoubtedly,this review provides valuable guidance for the efficient and high-value recovery of failed cathode materials.
基金supported by the National Natural Science Foundation of China(52127801)Postdoctoral Fellowship Program of CPSF under Grant Number GZC20231545,China Postdoctoral Science Foundation(2024T170557 and 2023M742224)+1 种基金Shanghai Post-doctoral Excellence Program(No.2023440)City University of Hong Kong Donation Grants(DON-RMG No.9229021 and 9220061).
文摘Pure Mg boasting a relatively small corrosion rate is a potential biodegradable metal material for implants.However,its degradation behavior in the complex physiological environment is still a lack of understanding.In this work,we investigated the effect of corrosion product film layers on the degradation behavior of pure Mg in physiological environments.Pure Mg shows a faster corrosion rate in simulated body fluid(SBF)compared to NaCl solution.Hydrogen evolution experiments indicate that the degradation rate of pure Mg in SBF decreases rapidly within the first 12 h but stabilizes afterward.The rapid deposition of low-solubility calcium phosphate on the pure Mg in SBF provides protection to the substrate,resulting in a gradual decrease in the degradation rates.Consequently,the corrosion product film of pure Mg formed in SBF exhibits a layered structure,with the upper layer consisting of dense Ca_(3)(PO_(4))_(2)/Mg_(3)(PO_(4))_(2) and the lower layer consisting of Mg(OH)_(2)/MgO.Electrochemical impedance spectroscopy(EIS)shows that the resistance of the corrosion product film increases over time,indicating gradual strengthening of the corrosion resistance.The 4-week degradation results in the femoral marrow cavity of mice are consistent with the result in SBF in vitro.
基金supported by the National Natural Science Foundation of China(Nos.U20A20274 and 52061003)the Natural Science Foundation of Yunnan Province(No.202301AT070209)the Science and Technology Major Project of Yunnan Province(No.202102AG050017).
文摘In this paper,the property degradation micromechanism of Al-5.10Cu-0.65 Mg-0.8Mn(wt%)alloy induced by 0.5 wt%Fe minor addition was revealed by atomic-scale scanning transmission electron microscopy and energy-dispersive X-ray spectroscopy coupled with first-principles calculations.The results show that the Fe minor addition to the Al-Cu-Mg-Mn alloy leads to a slight reduction of grain size and the formation of coarse Al7Cu2Fe constituent particles.Fe tends to segregate into the T-phase dispersoids,θ'-,and S-phase precipitates by preferentially occupying Cu or Mn sites in these phase structures.The apparent Fe segregation contributes to an increase in stiffness of the T-phase and S-phase but decreased stiffness of theθ'phase.Formation of the coarse Al7Cu2Fe constituent particles and decreased stiffness of main precipitatesθ'containing Fe result in the degraded strength of the Al-Cu-Mg-Mn-Fe alloy.Further study reveals that corrosion resistance degradation of the Al-Cu-Mg-Mn-Fe alloy is associated with the increased width of precipitation free zones and consecutive grain boundary precipitates.The obtained results have significant implications for the usage of recycled Al alloys and the potential design strategies of high-performance alloys containing Fe.
文摘Solar-driven Fenton-like reactions are promising strategies for degrading pharmaceutical wastewater to address environmental challenges and antibiotic pollution.However,its efficacy is limited by suboptimal light absorption efficiency,rapid charge recombination,and inadequate interfacial charge transfer.In this study,an inorganic/organic S-scheme photo-Fenton system of pseudobrookite/carbon nitride(FTOCN)was synthesized via a hydrothermally coupled calcination process for the effective purification of tetracycline antibiotics under visible-light irradiation.The optimized FTOCN-2 heterostructure exhibits a significantly enhanced TC degradation capacity of 90%within 60 min.The rate constant of FTOCN-2 is 1.6 and 5.2 times greater than those of FTO and CN,respectively.Furthermore,FTOCN exhibits high antibacterial efficacy,highlighting its potential application in the purification of natural water.Measurements via a range of analytical techniques,including Kelvin probe force microscopy,density functional theory calculations,in situ X-ray photoelectron spectroscopy,and femtosecond transient absorption spectroscopy,corroborate the S-scheme mechanism.This study provides a novel perspective for the development of photo-Fenton systems with S-scheme heterojunctions for water purification.
基金financially supported by the Jilin Province Development and Reform Commission(Innovation Capacity Building)Project,China(20231036-3)the Key R&D Project of the Science and Technology Development Plan of Jilin,China(20230203014SF)。
文摘Nicosulfuron(NSR),a sulfonylurea herbicide,readily infiltrates water bodies,potentially compromising aquatic ecosystems and human health.In this study,bacteria consortium YM2 was isolated and cultivated from pesticide plant active sludge for NSR wastewater bioremediation.Response surface methodology analysis demonstrated that under optimal cultivation conditions(9.41 g L^(-1)maltodextrin,21.37 g L^(-1)yeast extract,and 12.45 g L^(-1)NaCl),the YM2 bacteria consortium achieved 97.49%NSR degradation within 4 d.Optimal degradation parameters were established at 30℃,pH 6.0,1%inoculum,and 20 mg L^(-1)initial NSR concentration.The degradation system demonstrated resistance to heavy metal ions including Cd^(2+),Pb^(2+),Ni^(2+),and Zn^(2+),with degradation primarily occurring through bacterial extracellular enzymes(92.17%).During the degradation process,reactive oxygen species,oxidative stress,cell membrane permeability,cell surface hydrophobicity,and apoptosis rate exhibited initial increases followed by decreases.Additionally,bioflm formation-related genes luxS,waaE,spo0A,and wza showed temporal and concentration-dependent expression patterns.NSR concentrations in wastewater and soil were reduced to 1.92 and 2.72 mg L^(-1),respectively.In a simulated wastewater treatment unit with a 12-h hydraulic retention time,YM2 achieved 84.55%NSR degradation after 10 d.These fndings provide a theoretical foundation for microbial remediation of NSR contamination.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.31830071,32202561)the earmarked fund for CARS(Grant No.CARS-31)。
文摘Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation,epigenetic factor,and post-translational modification.Previously,we reported that MaERF11 cooperated with MaHDA1 to precisely regulate the transcription of ripening-associated genes via histone deacetylation.However,whether MaERF11 is subjected to post-translational modification during banana ripening is largely unknown.In this study,we found that MaERF11 targeted a subset of starch degradation-related genes using the DNA affinity purification sequence(DAP-Seq)approach.Electrophoretic mobility shift assay(EMSA)and dual-luciferase reporter assay(DLR)demonstrated that MaERF11 could specifically bind and repress the expression of the starch degradation-related genes MaAMY3,MaBAM2 and MaGWD1.Further analyses of yeast two-hybrid(Y2H),bimolecular fluorescence complementation(BiFC)and Luciferase complementation imaging(LCI)assays indicated that MaERF11 interacted with the ubiquitin E3 ligase MaRFA1,and this interaction weakened the MaERF11-mediated transcriptional repression capacity.Collectively,our results suggest an additional regulatory layer in which MaERF11 regulates banana fruit ripening and expands the regulatory network in fruit ripening at the post-translational modification level.
文摘At present,carbon capture and storage(CCS)is the only mature and commercialized technology capable of effectively and economically reducing greenhouse gas emissions to achieve a significant and immedi-ate impact on the CO_(2) level on Earth.Notably,long-term geological storage of captured CO_(2) has emerged as a primary storage method,given its minimal impact on surface ecological environments and high level of safety.The integrity of CO_(2) storage wellbores can be compromised by the corrosion of steel casings and degradation of cement in supercritical CO_(2) storage environments,potentially leading to the leakage of stored CO_(2) from the sites.This critical review endeavors to establish a knowledge foundation for the cor-rosion and materials degradation associated with geological CO_(2) storage through an in-depth examina-tion and analysis of the environments,operation,and the state-of-the-art progress in research pertaining to the topic.This article discusses the physical and chemical properties of CO_(2) in its supercrit-ical phase during injection and storage.It then introduces the principle of geological CO_(2) storage,consid-erations in the construction of storage systems,and the unique geo-bio-chemical environment involving aqueous media and microbial communities in CO_(2) storage.After a comprehensive analysis of existing knowledge on corrosion in CO_(2) storage,including corrosion mechanisms,parametric effects,and corro-sion rate measurements,this review identifies technical gaps and puts forward potential avenues for fur-ther research in steel corrosion within geological CO_(2) storage systems.
基金financially supported by the National Key Research and Development Program of China(No.2023YFF0612601)the Key Research and Development Program of Zhejiang Province(No.2023C02038)+2 种基金the Key Research and Development Program of Ningbo(No.2022Z178)China Construction Technology Research and Development Project(No.CSCEC-2021-Z-5)Zhejiang Provincial Natural Science Foundation of China(No.LQ23B010003)
文摘High-concentration single-atom doping remains a formidable challenge due to the propensity for single atoms to form clusters or aggregate at elevated concentrations.Herein,high-concentration (10.8 wt%) Zn singleatom-doped porous tubular g-C_(3)N_(4)(ZCN) was successfully obtained via a template-free,one-step calcination method,exhibiting excellent photocatalytic performance.The confinement of the pore walls suppresses the Zn atom'smigration and aggregation,enhancing the Zn single-atom stability.ZCN exhibited excellent photodegradation performance against tetracycline with outstanding stability.Moreover,ZCN displayed remarkable sterilization performance,achieving a 100%inactivation of Staphylococcus aureus within 90 min of visible-light exposure.Density functional theory calculations demonstrated that the Zn single-atom sites act as pivotal photocatalytic active sites,with the presence of Zn single atoms notably augmenting charge separation efficiency.This work provides a novel approach for managing photocatalytic efficiency through enlarging single-atom doping,offering an avenue for pollutant photodegradation and sterilization.
基金Financial support from the National Natural Science Foundation of China(51972016)the Fundamental Research Funds for the Central Universities(JD2417)is gratefully acknowledged.
文摘Although solar steam generation strategy is efficient in desalinating seawater,it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic pollutants.Herein,dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co_(2)(OH)_(2)CO_(3)nanorod top layer and a bacterial cellulose/Co_(2)(OH)_(2)CO_(3)nanorod(BCH)bottom layer.Crucially,the hydrogen bonding networks inside the membrane can be tuned by the rich surface–OH groups of the bacterial cellulose and Co_(2)(OH)_(2)CO_(3)as well as the ions and radicals in situ generated during the catalysis process.Moreover,both SO_(4)^(2−)and HSO_(5)−can regulate the solvation structure of Na^(+)and be adsorbed more preferentially on the evaporation surface than Cl^(−),thus hindering the de-solvation of the solvated Na^(+)and subsequent nucleation/growth of NaCl.Furthermore,the heat generated by the solar-thermal energy conversion can accelerate the reaction kinetics and enhance the catalytic degradation efficiency.This work provides a flow-bed water purification system with an asymmetric solar-thermal and catalytic membrane for synergistic solar thermal desalination of seawater/brine and catalytic degradation of organic pollutants.
