A plasmid for cytoglobin expression, pAcGFP1-Cl-cytoglobin, was transfected into SH-SY5Y cells. Cobalt chloride was used to establish a model of hypoxia. Western blotting indicated that cytoglobin was overexpressed an...A plasmid for cytoglobin expression, pAcGFP1-Cl-cytoglobin, was transfected into SH-SY5Y cells. Cobalt chloride was used to establish a model of hypoxia. Western blotting indicated that cytoglobin was overexpressed and there was low expression of hypoxia-inducible factor-la in SH-SY5Y cells after transfection. Following cobalt chloride-induced hypoxia, cytoglobin and hypoxia-inducible fac- tor-la expression gradually increased in SH-SY5Y cells. Flow cytometry showed that with increas- ing duration of hypoxia, the proportion of normal cells significantly diminished in the transfected and non-transfected groups. The proportion of cells in the early stages of apoptosis increased. However, the proportion of apoptotic cells was significantly lower in the transfected group compared with the non-transfected group. These results demonstrate that cytoglobin and hypoxia-inducible factor-la are strongly up-regulated by hypoxia, and that there is a strong relationship between hy- poxia-inducible factor-la and cytoglobin during hypoxic injury.展开更多
文摘A plasmid for cytoglobin expression, pAcGFP1-Cl-cytoglobin, was transfected into SH-SY5Y cells. Cobalt chloride was used to establish a model of hypoxia. Western blotting indicated that cytoglobin was overexpressed and there was low expression of hypoxia-inducible factor-la in SH-SY5Y cells after transfection. Following cobalt chloride-induced hypoxia, cytoglobin and hypoxia-inducible fac- tor-la expression gradually increased in SH-SY5Y cells. Flow cytometry showed that with increas- ing duration of hypoxia, the proportion of normal cells significantly diminished in the transfected and non-transfected groups. The proportion of cells in the early stages of apoptosis increased. However, the proportion of apoptotic cells was significantly lower in the transfected group compared with the non-transfected group. These results demonstrate that cytoglobin and hypoxia-inducible factor-la are strongly up-regulated by hypoxia, and that there is a strong relationship between hy- poxia-inducible factor-la and cytoglobin during hypoxic injury.
