In this paper, we present the study, modelling and simulation of the duty cycle modulation (DCM) based on SVPWM control technique using Matlab/Simulink software. It is one of the most advanced control techniques of sp...In this paper, we present the study, modelling and simulation of the duty cycle modulation (DCM) based on SVPWM control technique using Matlab/Simulink software. It is one of the most advanced control techniques of space vector modulation (SVM), which can be used for controlling static converters or for controlling electrical machines to achieve better dynamic performance. DCSVM is a control technique that generates control signals for the two-level voltage converter as well as for the intermediate times. The main advantage of this control technique is the reduction of the number of calculations, especially for the trigonometric functions and the generation of the reference voltage. In order to reduce the computational effort, we have designed a DCSVM controller that is able to faithfully reproduce the same vectors and output quantities as a classical SVM. In order to test the functionality and validity of the DCSVM control, we have developed different simulations that result in a total harmonic distortion (THD) of the voltage and current of 41.19% and 15.19% respectively with fundamental values of 61.51 V for the voltage and 2.80 A for the current;in contrast to the SVM which gives 47.27 V for the voltage and 2.01 A for the current with THDs of 77.16% for the voltage and 16.00% for the current. This results in an improvement in the distortion rate of around 25.5%. The results obtained are very satisfactory. The DCSVM is a real competitor to the SVM and its various variants.展开更多
Objective To study the effects of the p16 gene on tumor cell growth inhibition and cell cycle arrest. Methods The recombinant retroviral vector pLp16SN was constructed by cloning the human p16 cDNA into the retroviral...Objective To study the effects of the p16 gene on tumor cell growth inhibition and cell cycle arrest. Methods The recombinant retroviral vector pLp16SN was constructed by cloning the human p16 cDNA into the retroviral vector pLXSN. Retroviruses with or without the p16 gene were obtained by transfecting pLp16SN and pLXSN vectors into PA317 cells. Bcap-37 human breast cancer cells were infected with these retroviruses followed by selection with G418. The expression of p16 was detected by Northern and Westem blots. Cell biological characteristics, including cell growth rate, cell cycle and tumorigenesis in nude mice were assessed.Results Both mRNA and protein expression of p16 in Bcap-37 cells transfected with retroviral vector containing the p16 gene were much higher than that in Bcap-37 cells transfected with empty vector or parental Bcap-37 cells. Cell overexpressing the p16 gene exhibited a slower rate of growth, a higher percentage of cells in the G1 phase, and smaller tumors in nude mice, compared with parental Bcap-37 cells and cells transfected with empty vector.Conclusion Overexpression of the p16 gene could suppress the growth of Bcap-37 breast cancer cells by arresting the cell cvcle at the G1 to S-phase.展开更多
文摘In this paper, we present the study, modelling and simulation of the duty cycle modulation (DCM) based on SVPWM control technique using Matlab/Simulink software. It is one of the most advanced control techniques of space vector modulation (SVM), which can be used for controlling static converters or for controlling electrical machines to achieve better dynamic performance. DCSVM is a control technique that generates control signals for the two-level voltage converter as well as for the intermediate times. The main advantage of this control technique is the reduction of the number of calculations, especially for the trigonometric functions and the generation of the reference voltage. In order to reduce the computational effort, we have designed a DCSVM controller that is able to faithfully reproduce the same vectors and output quantities as a classical SVM. In order to test the functionality and validity of the DCSVM control, we have developed different simulations that result in a total harmonic distortion (THD) of the voltage and current of 41.19% and 15.19% respectively with fundamental values of 61.51 V for the voltage and 2.80 A for the current;in contrast to the SVM which gives 47.27 V for the voltage and 2.01 A for the current with THDs of 77.16% for the voltage and 16.00% for the current. This results in an improvement in the distortion rate of around 25.5%. The results obtained are very satisfactory. The DCSVM is a real competitor to the SVM and its various variants.
文摘Objective To study the effects of the p16 gene on tumor cell growth inhibition and cell cycle arrest. Methods The recombinant retroviral vector pLp16SN was constructed by cloning the human p16 cDNA into the retroviral vector pLXSN. Retroviruses with or without the p16 gene were obtained by transfecting pLp16SN and pLXSN vectors into PA317 cells. Bcap-37 human breast cancer cells were infected with these retroviruses followed by selection with G418. The expression of p16 was detected by Northern and Westem blots. Cell biological characteristics, including cell growth rate, cell cycle and tumorigenesis in nude mice were assessed.Results Both mRNA and protein expression of p16 in Bcap-37 cells transfected with retroviral vector containing the p16 gene were much higher than that in Bcap-37 cells transfected with empty vector or parental Bcap-37 cells. Cell overexpressing the p16 gene exhibited a slower rate of growth, a higher percentage of cells in the G1 phase, and smaller tumors in nude mice, compared with parental Bcap-37 cells and cells transfected with empty vector.Conclusion Overexpression of the p16 gene could suppress the growth of Bcap-37 breast cancer cells by arresting the cell cvcle at the G1 to S-phase.
