Liquid-filled compartment structure consists of a bulk steel plate with matrix blind holes which are filled with liquid and a steel front plate to seal up the liquid with rings and bolts.The liquid-filled compartment ...Liquid-filled compartment structure consists of a bulk steel plate with matrix blind holes which are filled with liquid and a steel front plate to seal up the liquid with rings and bolts.The liquid-filled compartment structure can resist the shaped charge warhead effectively.This paper presents experimental and theoretical investigations of the penetration ability of the residual shaped charge jet emerging from the liquid-filled compartment structure after the penetration process at different impact angles.On the basis of shock wave propagation theory,the influence of the liquid-filled compartment structure on jet stability is analysed.The interferences of the liquid backflow caused by a reflected shock wave and a back plate on jet stability under different impact angles are also examined.In addition,the range of the disturbed velocity segments of the jet at different impact angles and the penetration ability of the residual jet are obtained.A theoretical model is validated against the experimental penetration depths.展开更多
Super-resolution microscopy has revolutionized our ability to visualize structures below the diffraction limit of conventional optical microscopy and is particularly useful for investigating complex biological targets...Super-resolution microscopy has revolutionized our ability to visualize structures below the diffraction limit of conventional optical microscopy and is particularly useful for investigating complex biological targets like chromatin.Chromatin exhibits a hierarchical organization with structural compartments and domains at different length scales,from nanometers to micrometers.Single molecule localization microscopy(SMLM)methods,such as STORM,are essential for studying chromatin at the supra-nucleosome level due to their ability to target epigenetic marks that determine chromatin organization.Multi-label imaging of chromatin is necessary to unpack its structural complexity.However,these efforts are challenged by the high-density nuclear environment,which can affect antibody binding affinities,diffusivity and non-specific interactions.Optimizing buffer conditions,fluorophore stability,and antibody specificity is crucial for achieving effective antibody conjugates.Here,we demonstrate a sequential immunolabeling protocol that reliably enables three-color studies within the dense nuclear environment.This protocol couples multiplexed localization datasets with a robust analysis algorithm,which utilizes localizations from one target as seed points for distance,density and multi-label joint affinity measurements to explore complex organization of all three targets.Applying this multiplexed algorithm to analyze distance and joint density reveals that heterochromatin and euchromatin are not-distinct territories,but that localization of transcription and euchromatin couple with the periphery of heterochromatic clusters.This work is a crucial step in molecular imaging of the dense nuclear environment as multi-label capacity enables for investigation of complex multi-component systems like chromatin with enhanced accuracy.展开更多
基金This research was supported by the National Natural Science Foundation of China(Grant No.11472115,11872214)the China Scholarship Council(201706845026).
文摘Liquid-filled compartment structure consists of a bulk steel plate with matrix blind holes which are filled with liquid and a steel front plate to seal up the liquid with rings and bolts.The liquid-filled compartment structure can resist the shaped charge warhead effectively.This paper presents experimental and theoretical investigations of the penetration ability of the residual shaped charge jet emerging from the liquid-filled compartment structure after the penetration process at different impact angles.On the basis of shock wave propagation theory,the influence of the liquid-filled compartment structure on jet stability is analysed.The interferences of the liquid backflow caused by a reflected shock wave and a back plate on jet stability under different impact angles are also examined.In addition,the range of the disturbed velocity segments of the jet at different impact angles and the penetration ability of the residual jet are obtained.A theoretical model is validated against the experimental penetration depths.
基金supported by NIH grants U54CA268084,U54CA261694,and R01CA228272National Science Foundation grants EFMA-1830961 and CBET-2430743+1 种基金philanthropic support from Rob and Kristin Goldman,Mr.David Sachsthe Christina Carinato Charitable Foundation.
文摘Super-resolution microscopy has revolutionized our ability to visualize structures below the diffraction limit of conventional optical microscopy and is particularly useful for investigating complex biological targets like chromatin.Chromatin exhibits a hierarchical organization with structural compartments and domains at different length scales,from nanometers to micrometers.Single molecule localization microscopy(SMLM)methods,such as STORM,are essential for studying chromatin at the supra-nucleosome level due to their ability to target epigenetic marks that determine chromatin organization.Multi-label imaging of chromatin is necessary to unpack its structural complexity.However,these efforts are challenged by the high-density nuclear environment,which can affect antibody binding affinities,diffusivity and non-specific interactions.Optimizing buffer conditions,fluorophore stability,and antibody specificity is crucial for achieving effective antibody conjugates.Here,we demonstrate a sequential immunolabeling protocol that reliably enables three-color studies within the dense nuclear environment.This protocol couples multiplexed localization datasets with a robust analysis algorithm,which utilizes localizations from one target as seed points for distance,density and multi-label joint affinity measurements to explore complex organization of all three targets.Applying this multiplexed algorithm to analyze distance and joint density reveals that heterochromatin and euchromatin are not-distinct territories,but that localization of transcription and euchromatin couple with the periphery of heterochromatic clusters.This work is a crucial step in molecular imaging of the dense nuclear environment as multi-label capacity enables for investigation of complex multi-component systems like chromatin with enhanced accuracy.
