Rice-duck co-culture is an integrated farming technology that benefits rice production, grain quality, and ecological sustainability in paddy fields. However, little is known about the effects of rice-duck co-culture ...Rice-duck co-culture is an integrated farming technology that benefits rice production, grain quality, and ecological sustainability in paddy fields. However, little is known about the effects of rice-duck co-culture on enzyme activity involved in the biosynthesis of 2-acetyl-1-pyrroline (2-AP), the volatile that gives fragrant rice its' distinctive and sought-after aroma. The present study aimed to examine the influence of rice-duck co-culture on the photosynthesis, yield, grain quality, rice aroma, and the enzymes involved in 2-acetyl-1-pyrroline biosynthesis in the cultivar Meixiangzhan 2 during the early and late rice growing seasons of 2016 in Guangzhou, China. We compared the rice grown in paddy fields with and without ducks. We found that rice-duck co-culture not only improved the yield and quality of fragrant rice grain, but also promoted the precursors of 2-AP biosynthesis formation and 2-AP accumulation in the grain. Grain 2-AP content in rice-duck co-culture was noticeably increased with 9.60% and 20.81% in early and late seasons, respectively. Proline and pyrroline-5-carboxylic acid (P5C) (precursors of 2-AP biosynthesis) and the activity of enzymes such as proline dehydrogenase (ProDH), ornithine aminotransferase (OAT) and Δ1 pyrroline-5-carboxylic acid synthetase (P5CS) were all improved by 10.15%–12.99%, 32.91%–47.75%, 17.81%–26.71%, 6.25%–21.78%, and 10.58%–38.87% under rice-duck co-culture in both seasons, respectively. Overall, our results suggest that rice-duck co-culture is an environmentally-friendly and sustainable approach to improving rice aroma and grain quality of fragrant rice.展开更多
AP-2β是转录因子AP-2家族中的重要一员.AP-2是一个与哺乳动物的发育、细胞生长、分化、凋亡和肿瘤发生都有密切联系的重要的转录因子家族.最近的研究表明,AP-2能通过与其他蛋白的相互作用来调控AP-2下游基因的转录活性.采用免疫共沉淀...AP-2β是转录因子AP-2家族中的重要一员.AP-2是一个与哺乳动物的发育、细胞生长、分化、凋亡和肿瘤发生都有密切联系的重要的转录因子家族.最近的研究表明,AP-2能通过与其他蛋白的相互作用来调控AP-2下游基因的转录活性.采用免疫共沉淀结合质谱鉴定的方法筛选与AP-2β相互作用的蛋白.结果筛选到Stomatin like protein2(SLP-2)蛋白可能与AP-2β相互作用.免疫共沉淀实验证实外源过表达和内源的SLP-2蛋白都能与AP-2β蛋白相互作用.而且,细胞免疫荧光实验发现外源表达的Myc-AP-2β蛋白与内源的SLP-2蛋白在MCF-7细胞的胞质中有共定位.此外,免疫印记实验结果表明过表达SLP-2能上调AP-2β的蛋白水平,这就表明SLP-2与AP-2β相互作用后,SLP-2可能使AP-2β蛋白更稳定.这些研究结果为进一步研究这两个基因的功能提供了新的切入点.展开更多
基金supported by the Science and Technology Project of Guangdong Province (2015B090903077, 2016A020210094, 2017A090905030), Chinathe Science and Technology Project of Guangzhou (201604020062), China+1 种基金the Innovation Team Construction Project of Modern Agricultural Industry Technology System of Guangdong Province (2016LM1100), Chinathe Overseas Joint Doctoral Training Program of South China Agricultural University (2018LHPY010), China
文摘Rice-duck co-culture is an integrated farming technology that benefits rice production, grain quality, and ecological sustainability in paddy fields. However, little is known about the effects of rice-duck co-culture on enzyme activity involved in the biosynthesis of 2-acetyl-1-pyrroline (2-AP), the volatile that gives fragrant rice its' distinctive and sought-after aroma. The present study aimed to examine the influence of rice-duck co-culture on the photosynthesis, yield, grain quality, rice aroma, and the enzymes involved in 2-acetyl-1-pyrroline biosynthesis in the cultivar Meixiangzhan 2 during the early and late rice growing seasons of 2016 in Guangzhou, China. We compared the rice grown in paddy fields with and without ducks. We found that rice-duck co-culture not only improved the yield and quality of fragrant rice grain, but also promoted the precursors of 2-AP biosynthesis formation and 2-AP accumulation in the grain. Grain 2-AP content in rice-duck co-culture was noticeably increased with 9.60% and 20.81% in early and late seasons, respectively. Proline and pyrroline-5-carboxylic acid (P5C) (precursors of 2-AP biosynthesis) and the activity of enzymes such as proline dehydrogenase (ProDH), ornithine aminotransferase (OAT) and Δ1 pyrroline-5-carboxylic acid synthetase (P5CS) were all improved by 10.15%–12.99%, 32.91%–47.75%, 17.81%–26.71%, 6.25%–21.78%, and 10.58%–38.87% under rice-duck co-culture in both seasons, respectively. Overall, our results suggest that rice-duck co-culture is an environmentally-friendly and sustainable approach to improving rice aroma and grain quality of fragrant rice.
文摘AP-2β是转录因子AP-2家族中的重要一员.AP-2是一个与哺乳动物的发育、细胞生长、分化、凋亡和肿瘤发生都有密切联系的重要的转录因子家族.最近的研究表明,AP-2能通过与其他蛋白的相互作用来调控AP-2下游基因的转录活性.采用免疫共沉淀结合质谱鉴定的方法筛选与AP-2β相互作用的蛋白.结果筛选到Stomatin like protein2(SLP-2)蛋白可能与AP-2β相互作用.免疫共沉淀实验证实外源过表达和内源的SLP-2蛋白都能与AP-2β蛋白相互作用.而且,细胞免疫荧光实验发现外源表达的Myc-AP-2β蛋白与内源的SLP-2蛋白在MCF-7细胞的胞质中有共定位.此外,免疫印记实验结果表明过表达SLP-2能上调AP-2β的蛋白水平,这就表明SLP-2与AP-2β相互作用后,SLP-2可能使AP-2β蛋白更稳定.这些研究结果为进一步研究这两个基因的功能提供了新的切入点.
