目的:研究艰难梭菌感染(CDI)腹泻患者血清CXC趋化因子配体-10(CXCL-10)和白介素-27(IL-27)水平的变化及其与病情严重程度间的相关性。方法:将150例腹泻患者根据Cd培养结果是否阳性将其观察组(Cd培养阳性者,n=90)和对照组(Cd培养阴性者,n...目的:研究艰难梭菌感染(CDI)腹泻患者血清CXC趋化因子配体-10(CXCL-10)和白介素-27(IL-27)水平的变化及其与病情严重程度间的相关性。方法:将150例腹泻患者根据Cd培养结果是否阳性将其观察组(Cd培养阳性者,n=90)和对照组(Cd培养阴性者,n=60),观察组患者又根据CDI疾病严重程度(severity score index,SSI)评分将其分为轻度亚组(评分<4分,n=38)和中—重度亚组(评分≥4分,n=52)。所有人员进行血清CXCL-10和IL-27检测,比较三组人员血清CXCL-10和IL-27水平间的差异,并分析血清CXCL-10和IL-27与CDI腹泻严重程度间的相关性。结果:三组人员血清CXCL-10和IL-27水平高低为:观察组>对照组>健康组(P<0.05);观察组中—重度亚组患者血清CXCL-10和IL-27水平明显高于轻度亚组(P<0.05)。观察组SSI评分为(4.68±1.73)分,其中轻度亚组SSI评分为(3.02±0.89)分,中—重度亚组SSI评分为(5.13±1.09)分。Spearman直线相关性分析发现观察组患者血清CXCL-10和IL-27水平与SSI评分间呈正相关性(r=0.317和0.368,P=0.002和0.000)。ROC曲线分析发现:血清CXCL-10、IL-27及二者联合对CDI腹泻的诊断曲线下面积(area under curve,AUC)分别为:0.846、0.853和0.874,诊断敏感性分别为85.25%、80.30%和82.75%,诊断特异性分别78.50%、82.55%和85.85%。结论:血清CXCL-10和IL-27有助于艰难梭菌感染腹泻患者病情严重程度的判断,具临床应用价值。展开更多
CXCL-10 known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine 10 is a 8.7 kDa protein, which is secreted in response to IFN-γ by monocytes, endothelial cells and fi-broblasts. It has chemo-...CXCL-10 known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine 10 is a 8.7 kDa protein, which is secreted in response to IFN-γ by monocytes, endothelial cells and fi-broblasts. It has chemo-attraction for monocytes/macrophages, T cells, NK cells and dendritic cells in promotion of T cell adhesion to endothelial cells. In the present study, we investigated whether polymorphisms in CXCL-10 gene have any role in the manifestation of Tuberculous (TB) pleurisy. Two SNPs in CXCL-10 promoter region (﹣1447A > G and ﹣135G > A) were genotyped in patients with TB Pleurisy (n = 186), Pulmonary TB patients (n = 159) and healthy controls (n = 205) by PCR-RFLP. Disease associations were statistically analyzed by Fisher exact test. At the ﹣135G > A position, the frequencies of genotype GA and allele G were significantly high in TB pleurisy patients compared to healthy controls. While the frequencies of genotype AA and allele A were significantly low in TB pleurisy patients compared to healthy controls. The frequency of haplotype A-G with the combination of 1447A > G and ﹣135G > A was significantly high in TB pleurisy. Our results reveal that genotype GA and allele G at ﹣135G > A position were strongly associated with susceptibility to tuberculous pleurisy. The GA genotype may be a useful genetic marker for early detection of the disease in high risk individuals.展开更多
To investigate interferon-γ-inducible protein-10’s (IP-10) potential to anticipate rapid (RVR)- and sustained virological responses (SVR) to chronic hepatitis C (CHC) treatment. METHODSWe included case series examin...To investigate interferon-γ-inducible protein-10’s (IP-10) potential to anticipate rapid (RVR)- and sustained virological responses (SVR) to chronic hepatitis C (CHC) treatment. METHODSWe included case series examining RVR or SVR in relation to 24 or 48 wk treatment for CHC, in patients treatment free for at least six months, with genotype 1 or 4, and in relation to 24 wk treatment for genotype 2 and 3, with pegylated interferon in combination with ribavirin. Patients had to have both a baseline IP-10 level as well as a hepatitis C virus (HCV)-RNA determination 4 wk after treatment initiation or 24 wk after end of treatment. Studies including patients with liver diseases other than CHC, human immunodeficiency virus-infection, treatment with immunosuppresents or cytostatica, alcohol dependency or active intravenous drug-use were excluded. We found 81 articles by searching the MEDLINE and EMBASE databases. Eight studies were eligible for inclusion. Their quality were assesed using an 18 point checklist for case series, developed using a modified Delphi technique. Information was extracted from the articles, and no raw data was requisitioned. The review protocol was registered at the International Prospective Register of Systematic Reviews (reg. number: CRD42014008736). RESULTSThree studies reported on baseline IP-10 level in association with RVR. A signigficant association was found for HCV genotype 1 infection by two studies. Only two studies reported on HCV genotype 4 infected and genotype 2 and 3 infected patients, respectively. A trend was seen for an association between RVR and baseline IP-10 for genotype 4, while no association was found for genotype 2 and 3. Seven studies provided information regarding baseline IP-10 and SVR. Following the pattern regarding rapid virological response all five studies examining SVR in relation to baseline IP-10 levels for HCV, genotype 1 infected patients showed a significant association. Likewise a significant association was seen for HCV, genotype 4 infected, while no association was found for HCV, genotype 2 and 3 infected. Though only two studies examined the assosiation for HCV genotype 4 infected and HCV genotype 2 and 3 infected respectively. CONCLUSIONWe found indications of a possible association between baseline IP-10 level and virological responses in patients with CHC genotype 1 and 4.展开更多
文摘目的:研究艰难梭菌感染(CDI)腹泻患者血清CXC趋化因子配体-10(CXCL-10)和白介素-27(IL-27)水平的变化及其与病情严重程度间的相关性。方法:将150例腹泻患者根据Cd培养结果是否阳性将其观察组(Cd培养阳性者,n=90)和对照组(Cd培养阴性者,n=60),观察组患者又根据CDI疾病严重程度(severity score index,SSI)评分将其分为轻度亚组(评分<4分,n=38)和中—重度亚组(评分≥4分,n=52)。所有人员进行血清CXCL-10和IL-27检测,比较三组人员血清CXCL-10和IL-27水平间的差异,并分析血清CXCL-10和IL-27与CDI腹泻严重程度间的相关性。结果:三组人员血清CXCL-10和IL-27水平高低为:观察组>对照组>健康组(P<0.05);观察组中—重度亚组患者血清CXCL-10和IL-27水平明显高于轻度亚组(P<0.05)。观察组SSI评分为(4.68±1.73)分,其中轻度亚组SSI评分为(3.02±0.89)分,中—重度亚组SSI评分为(5.13±1.09)分。Spearman直线相关性分析发现观察组患者血清CXCL-10和IL-27水平与SSI评分间呈正相关性(r=0.317和0.368,P=0.002和0.000)。ROC曲线分析发现:血清CXCL-10、IL-27及二者联合对CDI腹泻的诊断曲线下面积(area under curve,AUC)分别为:0.846、0.853和0.874,诊断敏感性分别为85.25%、80.30%和82.75%,诊断特异性分别78.50%、82.55%和85.85%。结论:血清CXCL-10和IL-27有助于艰难梭菌感染腹泻患者病情严重程度的判断,具临床应用价值。
文摘CXCL-10 known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine 10 is a 8.7 kDa protein, which is secreted in response to IFN-γ by monocytes, endothelial cells and fi-broblasts. It has chemo-attraction for monocytes/macrophages, T cells, NK cells and dendritic cells in promotion of T cell adhesion to endothelial cells. In the present study, we investigated whether polymorphisms in CXCL-10 gene have any role in the manifestation of Tuberculous (TB) pleurisy. Two SNPs in CXCL-10 promoter region (﹣1447A > G and ﹣135G > A) were genotyped in patients with TB Pleurisy (n = 186), Pulmonary TB patients (n = 159) and healthy controls (n = 205) by PCR-RFLP. Disease associations were statistically analyzed by Fisher exact test. At the ﹣135G > A position, the frequencies of genotype GA and allele G were significantly high in TB pleurisy patients compared to healthy controls. While the frequencies of genotype AA and allele A were significantly low in TB pleurisy patients compared to healthy controls. The frequency of haplotype A-G with the combination of 1447A > G and ﹣135G > A was significantly high in TB pleurisy. Our results reveal that genotype GA and allele G at ﹣135G > A position were strongly associated with susceptibility to tuberculous pleurisy. The GA genotype may be a useful genetic marker for early detection of the disease in high risk individuals.
基金Supported by Amagar and Hvidovre Hospital Research Foun-dation of 45000 Dkr.(to Bastian Neesgaard)The Family Hede Nielsen Foundation of 10000 Dkr.(to Bastian Neesgaard)
文摘To investigate interferon-γ-inducible protein-10’s (IP-10) potential to anticipate rapid (RVR)- and sustained virological responses (SVR) to chronic hepatitis C (CHC) treatment. METHODSWe included case series examining RVR or SVR in relation to 24 or 48 wk treatment for CHC, in patients treatment free for at least six months, with genotype 1 or 4, and in relation to 24 wk treatment for genotype 2 and 3, with pegylated interferon in combination with ribavirin. Patients had to have both a baseline IP-10 level as well as a hepatitis C virus (HCV)-RNA determination 4 wk after treatment initiation or 24 wk after end of treatment. Studies including patients with liver diseases other than CHC, human immunodeficiency virus-infection, treatment with immunosuppresents or cytostatica, alcohol dependency or active intravenous drug-use were excluded. We found 81 articles by searching the MEDLINE and EMBASE databases. Eight studies were eligible for inclusion. Their quality were assesed using an 18 point checklist for case series, developed using a modified Delphi technique. Information was extracted from the articles, and no raw data was requisitioned. The review protocol was registered at the International Prospective Register of Systematic Reviews (reg. number: CRD42014008736). RESULTSThree studies reported on baseline IP-10 level in association with RVR. A signigficant association was found for HCV genotype 1 infection by two studies. Only two studies reported on HCV genotype 4 infected and genotype 2 and 3 infected patients, respectively. A trend was seen for an association between RVR and baseline IP-10 for genotype 4, while no association was found for genotype 2 and 3. Seven studies provided information regarding baseline IP-10 and SVR. Following the pattern regarding rapid virological response all five studies examining SVR in relation to baseline IP-10 levels for HCV, genotype 1 infected patients showed a significant association. Likewise a significant association was seen for HCV, genotype 4 infected, while no association was found for HCV, genotype 2 and 3 infected. Though only two studies examined the assosiation for HCV genotype 4 infected and HCV genotype 2 and 3 infected respectively. CONCLUSIONWe found indications of a possible association between baseline IP-10 level and virological responses in patients with CHC genotype 1 and 4.
