目的探讨CMTM6、PD-L1在子宫内膜异位症(endometriosis,EM)中的表达及意义。方法收集50例EM患者腹腔镜下手术切除的卵巢异位子宫内膜组织、刮除的在位子宫内膜组织(实验组)以及30例非EM患者刮除的在位子宫内膜组织(对照组),采用免疫组...目的探讨CMTM6、PD-L1在子宫内膜异位症(endometriosis,EM)中的表达及意义。方法收集50例EM患者腹腔镜下手术切除的卵巢异位子宫内膜组织、刮除的在位子宫内膜组织(实验组)以及30例非EM患者刮除的在位子宫内膜组织(对照组),采用免疫组化法检测CMTM6、PD-L1的表达水平。收集实验组相关临床病理资料,包括患者年龄、体重指数(body mass index,BMI)、病灶大小、血清CA-125浓度及rAFS评分等,采用Spearman系数法分析CMTM6、PD-L1的表达与上述病理参数的相关性。培养人EM上皮细胞系12Z,采用Western blot及免疫蛋白共沉淀法检测CMTM6、PD-L1的表达水平及相互作用。结果CMTM6、PD-L1在EM患者中高表达,EM患者异位、在位子宫内膜中的表达水平高于非EM患者在位子宫内膜组织(P<0.05),CMTM6、PD-L1表达共同定位于异位、在位子宫内膜组织上皮细胞质、细胞膜中,且两种蛋白表达呈正相关(P<0.05)。患者年龄、BMI、病灶大小、血清CA-125浓度及rAFS评分与CMTM6、PD-L1的表达呈正相关(P<0.01)。CMTM6、PD-L1蛋白在12Z中表达,且CMTM6和PD-L能够相互结合。结论CMTM6、PD-L1在EM患者中高表达,CMTM6、PDL1的表达水平与EM病变严重程度相关,CMTM6、PD-L1在EM中能相互结合共同参与EM的发生、发展,CMTM6、PD-L1或可成为EM的潜在生物学标志物,为EM的临床诊治提供新靶点。展开更多
Objective:To investigate the correlation between miRNA,CMTM6,and PD-L1 expression in gastric cancer,providing new therapeutic targets for immunotherapy in gastric cancer.Methods:This study selected gastric cancer pati...Objective:To investigate the correlation between miRNA,CMTM6,and PD-L1 expression in gastric cancer,providing new therapeutic targets for immunotherapy in gastric cancer.Methods:This study selected gastric cancer patients who were diagnosed and treated at our hospital from October 2022 to October 2024 as the research subjects.Based on the patients’PD-L1 examination results,they were divided into a positive group and a negative group.General patient data were collected,and qPCR and WB experiments were used to detect the levels of CMTM6 and miRNA in the patients.Univariate analysis was conducted to identify factors influencing PD-L1 expression,and variables with p<0.05 were included in multivariate logistic regression analysis to clarify the correlation between miRNA,CMTM6,and PD-L1 expression in gastric cancer.Results:A total of 118 patients were included in this study,with 75 patients in the positive group and 43 patients in the negative group.Univariate analysis revealed that TNM stage,miRNA,and CMTM6 showed statistical significance in data comparison(p<0.05).These variables were then included in multivariate logistic regression analysis,which found that TNM stage(OR=2.849,95%CI:2.227-3.425),miRNA(OR=3.038,95%CI:2.968-3.509),and CMTM6(OR=3.185,95%CI:2.995-3.810)all exhibited a positive correlation with PD-L1 expression in gastric cancer.Conclusion:There is a certain correlation between miRNA,CMTM6,and PD-L1 expression in gastric cancer.As miRNA and CMTM6 levels increase,the positive rate of PD-L1 examination in patients also rises,warranting clinical attention.展开更多
文摘目的探讨CMTM6、PD-L1在子宫内膜异位症(endometriosis,EM)中的表达及意义。方法收集50例EM患者腹腔镜下手术切除的卵巢异位子宫内膜组织、刮除的在位子宫内膜组织(实验组)以及30例非EM患者刮除的在位子宫内膜组织(对照组),采用免疫组化法检测CMTM6、PD-L1的表达水平。收集实验组相关临床病理资料,包括患者年龄、体重指数(body mass index,BMI)、病灶大小、血清CA-125浓度及rAFS评分等,采用Spearman系数法分析CMTM6、PD-L1的表达与上述病理参数的相关性。培养人EM上皮细胞系12Z,采用Western blot及免疫蛋白共沉淀法检测CMTM6、PD-L1的表达水平及相互作用。结果CMTM6、PD-L1在EM患者中高表达,EM患者异位、在位子宫内膜中的表达水平高于非EM患者在位子宫内膜组织(P<0.05),CMTM6、PD-L1表达共同定位于异位、在位子宫内膜组织上皮细胞质、细胞膜中,且两种蛋白表达呈正相关(P<0.05)。患者年龄、BMI、病灶大小、血清CA-125浓度及rAFS评分与CMTM6、PD-L1的表达呈正相关(P<0.01)。CMTM6、PD-L1蛋白在12Z中表达,且CMTM6和PD-L能够相互结合。结论CMTM6、PD-L1在EM患者中高表达,CMTM6、PDL1的表达水平与EM病变严重程度相关,CMTM6、PD-L1在EM中能相互结合共同参与EM的发生、发展,CMTM6、PD-L1或可成为EM的潜在生物学标志物,为EM的临床诊治提供新靶点。
文摘Objective:To investigate the correlation between miRNA,CMTM6,and PD-L1 expression in gastric cancer,providing new therapeutic targets for immunotherapy in gastric cancer.Methods:This study selected gastric cancer patients who were diagnosed and treated at our hospital from October 2022 to October 2024 as the research subjects.Based on the patients’PD-L1 examination results,they were divided into a positive group and a negative group.General patient data were collected,and qPCR and WB experiments were used to detect the levels of CMTM6 and miRNA in the patients.Univariate analysis was conducted to identify factors influencing PD-L1 expression,and variables with p<0.05 were included in multivariate logistic regression analysis to clarify the correlation between miRNA,CMTM6,and PD-L1 expression in gastric cancer.Results:A total of 118 patients were included in this study,with 75 patients in the positive group and 43 patients in the negative group.Univariate analysis revealed that TNM stage,miRNA,and CMTM6 showed statistical significance in data comparison(p<0.05).These variables were then included in multivariate logistic regression analysis,which found that TNM stage(OR=2.849,95%CI:2.227-3.425),miRNA(OR=3.038,95%CI:2.968-3.509),and CMTM6(OR=3.185,95%CI:2.995-3.810)all exhibited a positive correlation with PD-L1 expression in gastric cancer.Conclusion:There is a certain correlation between miRNA,CMTM6,and PD-L1 expression in gastric cancer.As miRNA and CMTM6 levels increase,the positive rate of PD-L1 examination in patients also rises,warranting clinical attention.
