The expression of the cell death-inducing DNA fragmentation factorα-like effector（CIDE）family including Cidea,Cideb,and Cidec was significantly increased in mouse and human models of obesity.However,there was less ...The expression of the cell death-inducing DNA fragmentation factorα-like effector（CIDE）family including Cidea,Cideb,and Cidec was significantly increased in mouse and human models of obesity.However,there was less information on these genes’expression in pigs.Here,we hypothesized that different fat accumulation between lean（Duroc×Landrace×Yorkshire gilts,DLY）and obese（Lantang）pigs was attributed to porcine CIDE-modulating lipid metabolism.Our data showed that Cidea and Cidec were expressed at a high level in adipose tissue,and at a relatively high level in skeletal muscle,whereas Cideb was mainly expressed in the liver in both breeds of pig.Lantang pigs had higher white adipose and skeletal muscle Cidea and Cidec mR NA abundance,and hepatic and muscle Cideb mR NA than DLY pigs.Lipid metabolism-related genes including sterol regulatory element binding protein 1c（SREBP-1c）,hepatocyte nuclear factor-4α（HNF-4α）,peroxisome proliferator-activated receptorγcoactivator-1α（PGC-1α）,fatty acid synthase（FASN）,diacylglycerol O-acyltransferase 1（DGAT1）,and DGAT2 showed a higher expression level in adipose tissue from obese pigs than in that from lean pigs.Lantang pigs exhibited higher mR NA abundance for liver SREBP-1c,HNF-4α,and PGC-1α,and higher skeletal muscle SREBP-1c,HNF-4α,PGC-1α,and DGAT2 expression,as compared with DLY pigs.However,the perlipin2 mR NA levels in adipose tissues,liver,and skeletal muscle were significantly lower in obese pigs than in their lean counterparts.Furthermore,plasma non-esterified fatty acid（NEFA）,glucose,and triacylglycerol（TAG）levels were greater in obese pigs than in lean pigs.Finally,data from correlation analysis further found that CIDE mR NA expression was positively correlated with back fat thickness（BFT）,abdominal fat mass（AFM）,and the levels of NEFA,TAG,and glucose in the two breeds.Collectively,these data revealed that the porcine CIDEs possibly modulated lipid metabolism and contributed to the development of fat deposition and obesity in Lantang pigs.展开更多
基金supported by the National Natural Science Foundation of China(No.31402086)the China Agriculture Research System(CARS-36)+2 种基金the National Basic Research Program(973)of China(Nos.2013CB127301 and 2013CB127304)the Guangdong International Science and Technology Cooperation Program(No.2014A050503049)the Science and Technology Program of Guangdong Province(No.2016A020210041),China
文摘The expression of the cell death-inducing DNA fragmentation factorα-like effector（CIDE）family including Cidea,Cideb,and Cidec was significantly increased in mouse and human models of obesity.However,there was less information on these genes’expression in pigs.Here,we hypothesized that different fat accumulation between lean（Duroc×Landrace×Yorkshire gilts,DLY）and obese（Lantang）pigs was attributed to porcine CIDE-modulating lipid metabolism.Our data showed that Cidea and Cidec were expressed at a high level in adipose tissue,and at a relatively high level in skeletal muscle,whereas Cideb was mainly expressed in the liver in both breeds of pig.Lantang pigs had higher white adipose and skeletal muscle Cidea and Cidec mR NA abundance,and hepatic and muscle Cideb mR NA than DLY pigs.Lipid metabolism-related genes including sterol regulatory element binding protein 1c（SREBP-1c）,hepatocyte nuclear factor-4α（HNF-4α）,peroxisome proliferator-activated receptorγcoactivator-1α（PGC-1α）,fatty acid synthase（FASN）,diacylglycerol O-acyltransferase 1（DGAT1）,and DGAT2 showed a higher expression level in adipose tissue from obese pigs than in that from lean pigs.Lantang pigs exhibited higher mR NA abundance for liver SREBP-1c,HNF-4α,and PGC-1α,and higher skeletal muscle SREBP-1c,HNF-4α,PGC-1α,and DGAT2 expression,as compared with DLY pigs.However,the perlipin2 mR NA levels in adipose tissues,liver,and skeletal muscle were significantly lower in obese pigs than in their lean counterparts.Furthermore,plasma non-esterified fatty acid（NEFA）,glucose,and triacylglycerol（TAG）levels were greater in obese pigs than in lean pigs.Finally,data from correlation analysis further found that CIDE mR NA expression was positively correlated with back fat thickness（BFT）,abdominal fat mass（AFM）,and the levels of NEFA,TAG,and glucose in the two breeds.Collectively,these data revealed that the porcine CIDEs possibly modulated lipid metabolism and contributed to the development of fat deposition and obesity in Lantang pigs.
