目的探究CDC40在结直肠癌组织中的表达特征及其与临床病理特征的相关性,为结直肠癌的早期诊断、靶向治疗及预后评估提供依据。方法采用免疫组织化学染色评估70例结直肠癌患者组织中CDC40表达水平,并分析其与临床病理特征关系;运用R软件...目的探究CDC40在结直肠癌组织中的表达特征及其与临床病理特征的相关性,为结直肠癌的早期诊断、靶向治疗及预后评估提供依据。方法采用免疫组织化学染色评估70例结直肠癌患者组织中CDC40表达水平,并分析其与临床病理特征关系;运用R软件包进行生存曲线和多因素预后分析;使用结直肠癌细胞系SW620和Caco-2进行细胞培养、CDC40质粒转染、细胞增殖实验、蛋白质印迹及裸鼠成瘤实验,以验证CDC40对细胞增殖的影响,实验数据采用IBM SPSS Statistics Version 22软件进行统计分析。结果70例患者中CDC40高表达组占51.4%,其表达高低与肿瘤分化程度、T分期、淋巴结转移及增殖指数Ki67水平相关,与性别、年龄、远处转移无关;多因素和单因素生存分析显示CDC40高表达与患者更差的生存预后相关;体外实验中过表达CDC40促进结直肠癌细胞系增殖;裸鼠成瘤实验表明过表达CDC40促进肿瘤生长。结论CDC40在结直肠癌发生发展中起重要作用,可作为潜在分子标志物和治疗靶点。展开更多
说起新闻学,在信息化高度发达的今天,为了获得受众关注,其用一些夸张或是带有感情色彩的词汇本无可厚非,新闻稿件作者本人的情感体现其中也属正常。国人,特别是科研人员对于《Science》、《Nature》等顶级期刊学术影响力的认可,使得我...说起新闻学,在信息化高度发达的今天,为了获得受众关注,其用一些夸张或是带有感情色彩的词汇本无可厚非,新闻稿件作者本人的情感体现其中也属正常。国人,特别是科研人员对于《Science》、《Nature》等顶级期刊学术影响力的认可,使得我们对《Science》在线新闻提供的信息格外关注。2025年11月21日《Science》以在线新闻的形式提供了这样一个报道“Exclusive:CDC to end all monkey research”,副标题为“Studies related to HIV and other infectious diseases will be phased out,sources say;fate of the agency’s animals remains unclear”,作者是《Science》杂志的在线新闻编辑大卫·格里姆[1]。展开更多
Background:Lung adenocarcinoma,the most prevalent lung cancer subtype,is increasingly diagnosed in non-smokers and females.The cell cycle regulator CDC20(Cell Division Cycle 20),a crucial activator of the Anaphase-Pro...Background:Lung adenocarcinoma,the most prevalent lung cancer subtype,is increasingly diagnosed in non-smokers and females.The cell cycle regulator CDC20(Cell Division Cycle 20),a crucial activator of the Anaphase-Promoting Complex/Cyclosome(APC/C),is frequently overexpressed in various cancers,including lung adenocarcinoma,and is implicated in tumorigenesis.Preclinical studies indicate that inhibiting the CDC20-APC/C signaling axis can enhance chemotherapy-induced apoptosis.Analysis of The Cancer Genome Atlas(TCGA)data confirms that elevated CDC20 expression in lung adenocarcinoma is significantly associated with poorer patient prognosis and correlates with immune cell infiltration.These collective findings highlight CDC20 as a promising prognostic biomarker and a potential novel therapeutic target for lung adenocarcinoma.Methods:We collected 539 patients with LUAD and 59 normal controls of clinical data from the Cancer Genome Atlas(TCGA)for bioinformatics analysis to investigate the role of CDC20 in LUAD and address the above questions.We evaluated the association between CDC20 expression and clinicopathological features using the Kruskal Wallis test and multivariate logistic regression.Prognostic values were assessed using Cox regression and Kaplan-Meier survival analyses.We further used single-sample gene set enrichment analysis(ssGSEA)to explore correlations between CDC20 expression and immune infiltration levels.Results:CDC20 expression in LUAD tissues was significantly higher than that in normal controls(p<0.001)and showed high diagnostic accuracy(AUC[area under the curve]=0.979).Kaplan-Meier analysis revealed that high CDC20 expression predicted poorer overall survival(OS;HR=1.47,95%CI:1.10–1.97,p=0.009),although no significant association emerged with progression-free interval(p=0.172).ssGSEA indicated a strong positive correlation between CDC20 and T helper 2 cell infiltration(R=0.764,p<0.001),but negative correlations with mast cells(R=−0.469,p<0.001)and eosinophils(R=−0.343,p<0.001).Functional enrichment analyses(Gene ontology/Kyoto Encyclopedia of Genes and Genomes[GO/KEGG])of CDC20-associated genes provided additional mechanistic insights.Conclusions:The significantly elevated expression of CDC20 in LUAD tissues,coupled with its high diagnostic accuracy(AUC=0.979),underscores its potential utility in differentiating LUAD from normal tissue.More importantly,the strong association between high CDC20 expression and poorer overall survival(OS)establishes its independent prognostic value for predicting adverse patient outcomes.Beyond its correlation with clinical parameters,our findings illuminate potential mechanisms underlying CDC20's oncogenic role.The distinct positive correlation with T helper 2(Th2)cell infiltration and negative correlations with mast cells and eosinophils suggest that CDC20 may actively shape an immunosuppressive tumor microenvironment,potentially facilitating tumor immune evasion and progression.Functional enrichment analyses of CDC20-coexpressed genes further support its involvement in key oncogenic pathways,including cell cycle regulation and mitotic processes.Collectively,this study not only validates CDC20 as a valuable prognostic factor but also provides novel mechanistic insights linking its overexpression to altered immune landscapes in LUAD.展开更多
In the heart of Addis Ababa,where tradition meets modern ambition,a gleaming structure of steel and glass is quietly reshaping Africa’s future.The new Africa Centres for Disease Control and Prevention(Africa CDC)Head...In the heart of Addis Ababa,where tradition meets modern ambition,a gleaming structure of steel and glass is quietly reshaping Africa’s future.The new Africa Centres for Disease Control and Prevention(Africa CDC)Headquarters is more than just a building-it’s a bold statement of partnership,progress,and a shared commitment to advancing public health across the continent.展开更多
文摘目的探究CDC40在结直肠癌组织中的表达特征及其与临床病理特征的相关性,为结直肠癌的早期诊断、靶向治疗及预后评估提供依据。方法采用免疫组织化学染色评估70例结直肠癌患者组织中CDC40表达水平,并分析其与临床病理特征关系;运用R软件包进行生存曲线和多因素预后分析;使用结直肠癌细胞系SW620和Caco-2进行细胞培养、CDC40质粒转染、细胞增殖实验、蛋白质印迹及裸鼠成瘤实验,以验证CDC40对细胞增殖的影响,实验数据采用IBM SPSS Statistics Version 22软件进行统计分析。结果70例患者中CDC40高表达组占51.4%,其表达高低与肿瘤分化程度、T分期、淋巴结转移及增殖指数Ki67水平相关,与性别、年龄、远处转移无关;多因素和单因素生存分析显示CDC40高表达与患者更差的生存预后相关;体外实验中过表达CDC40促进结直肠癌细胞系增殖;裸鼠成瘤实验表明过表达CDC40促进肿瘤生长。结论CDC40在结直肠癌发生发展中起重要作用,可作为潜在分子标志物和治疗靶点。
文摘说起新闻学,在信息化高度发达的今天,为了获得受众关注,其用一些夸张或是带有感情色彩的词汇本无可厚非,新闻稿件作者本人的情感体现其中也属正常。国人,特别是科研人员对于《Science》、《Nature》等顶级期刊学术影响力的认可,使得我们对《Science》在线新闻提供的信息格外关注。2025年11月21日《Science》以在线新闻的形式提供了这样一个报道“Exclusive:CDC to end all monkey research”,副标题为“Studies related to HIV and other infectious diseases will be phased out,sources say;fate of the agency’s animals remains unclear”,作者是《Science》杂志的在线新闻编辑大卫·格里姆[1]。
基金funded by the Liaoning Province Science and Technology Plan Joint Project (No.2023JH2/101700140)
文摘Background:Lung adenocarcinoma,the most prevalent lung cancer subtype,is increasingly diagnosed in non-smokers and females.The cell cycle regulator CDC20(Cell Division Cycle 20),a crucial activator of the Anaphase-Promoting Complex/Cyclosome(APC/C),is frequently overexpressed in various cancers,including lung adenocarcinoma,and is implicated in tumorigenesis.Preclinical studies indicate that inhibiting the CDC20-APC/C signaling axis can enhance chemotherapy-induced apoptosis.Analysis of The Cancer Genome Atlas(TCGA)data confirms that elevated CDC20 expression in lung adenocarcinoma is significantly associated with poorer patient prognosis and correlates with immune cell infiltration.These collective findings highlight CDC20 as a promising prognostic biomarker and a potential novel therapeutic target for lung adenocarcinoma.Methods:We collected 539 patients with LUAD and 59 normal controls of clinical data from the Cancer Genome Atlas(TCGA)for bioinformatics analysis to investigate the role of CDC20 in LUAD and address the above questions.We evaluated the association between CDC20 expression and clinicopathological features using the Kruskal Wallis test and multivariate logistic regression.Prognostic values were assessed using Cox regression and Kaplan-Meier survival analyses.We further used single-sample gene set enrichment analysis(ssGSEA)to explore correlations between CDC20 expression and immune infiltration levels.Results:CDC20 expression in LUAD tissues was significantly higher than that in normal controls(p<0.001)and showed high diagnostic accuracy(AUC[area under the curve]=0.979).Kaplan-Meier analysis revealed that high CDC20 expression predicted poorer overall survival(OS;HR=1.47,95%CI:1.10–1.97,p=0.009),although no significant association emerged with progression-free interval(p=0.172).ssGSEA indicated a strong positive correlation between CDC20 and T helper 2 cell infiltration(R=0.764,p<0.001),but negative correlations with mast cells(R=−0.469,p<0.001)and eosinophils(R=−0.343,p<0.001).Functional enrichment analyses(Gene ontology/Kyoto Encyclopedia of Genes and Genomes[GO/KEGG])of CDC20-associated genes provided additional mechanistic insights.Conclusions:The significantly elevated expression of CDC20 in LUAD tissues,coupled with its high diagnostic accuracy(AUC=0.979),underscores its potential utility in differentiating LUAD from normal tissue.More importantly,the strong association between high CDC20 expression and poorer overall survival(OS)establishes its independent prognostic value for predicting adverse patient outcomes.Beyond its correlation with clinical parameters,our findings illuminate potential mechanisms underlying CDC20's oncogenic role.The distinct positive correlation with T helper 2(Th2)cell infiltration and negative correlations with mast cells and eosinophils suggest that CDC20 may actively shape an immunosuppressive tumor microenvironment,potentially facilitating tumor immune evasion and progression.Functional enrichment analyses of CDC20-coexpressed genes further support its involvement in key oncogenic pathways,including cell cycle regulation and mitotic processes.Collectively,this study not only validates CDC20 as a valuable prognostic factor but also provides novel mechanistic insights linking its overexpression to altered immune landscapes in LUAD.
文摘In the heart of Addis Ababa,where tradition meets modern ambition,a gleaming structure of steel and glass is quietly reshaping Africa’s future.The new Africa Centres for Disease Control and Prevention(Africa CDC)Headquarters is more than just a building-it’s a bold statement of partnership,progress,and a shared commitment to advancing public health across the continent.
