Objective : To develop a treatment strategy for restenosis with a replication-deficient recombinant adenovirus (AdCMVCD) containing the cd gent whose gene product phosphorylates the prodrug 5-flurocytosine to form a n...Objective : To develop a treatment strategy for restenosis with a replication-deficient recombinant adenovirus (AdCMVCD) containing the cd gent whose gene product phosphorylates the prodrug 5-flurocytosine to form a nucleoside analog that inhibits DNA synthesis. Methods: Cultured primary rabbit smooth muscle cell (SMC) infected with AdCMVCD was incubated in 5- flurocytosine-containing medium and assayed with a Coulter Counter on day 2, 6, 10, and 14 for the establish- ment of proliferation curves. In addition, to observe an inhibitory effect on neighboring SMC, only a portion of the SMC population received the cd transgene. Results : The antiproliferative effect of AdCMVCD was dependent on both concentation of virus and concentration of 5-flurocytosine . Transformed cells demonstrated a bystander effect wherein SMC expressing cd gene exerted an antiproliferative effect on neighboring cells that did not express cd gene . Conclusion:Theresult demonstrates the potential utility of adenovirus-mediated cd gene transfer for the treatment of restenosis after balloon injury.展开更多
在说话人识别研究中,基于身份认证向量(Identity vector,IVEC)的说话人建模方法可以有效地提取说话人信息,是目前处于国际前沿的建模方法.本文对身份认证向量后接支持向量机(Identity vector followed by support vector machine,IVEC-S...在说话人识别研究中,基于身份认证向量(Identity vector,IVEC)的说话人建模方法可以有效地提取说话人信息,是目前处于国际前沿的建模方法.本文对身份认证向量后接支持向量机(Identity vector followed by support vector machine,IVEC-SVM)的说话人识别系统进行了研究,对比了该系统在十种不同核函数下的识别性能,并与文献中身份认证向量后接余弦距离打分(Identity vector followed by cosine distance scoring,IVEC-CDS)系统进行了比较.在美国国家标准技术局(American National Institute of Standards and Technology,NIST)组织的2010年电话信道—电话信道说话人识别核心评测数据库上的实验结果显示,基于核函数的IVEC-SVM系统性能明显优于IVEC-CDS的系统性能.此外,实验结果表明基于Spline核的IVEC-SVM系统可取得最好的识别性能,与IVEC-CDS系统相比,其等错点(Equal error rate,EER)在分数归一化前后分别降低了10%和3%.展开更多
文摘Objective : To develop a treatment strategy for restenosis with a replication-deficient recombinant adenovirus (AdCMVCD) containing the cd gent whose gene product phosphorylates the prodrug 5-flurocytosine to form a nucleoside analog that inhibits DNA synthesis. Methods: Cultured primary rabbit smooth muscle cell (SMC) infected with AdCMVCD was incubated in 5- flurocytosine-containing medium and assayed with a Coulter Counter on day 2, 6, 10, and 14 for the establish- ment of proliferation curves. In addition, to observe an inhibitory effect on neighboring SMC, only a portion of the SMC population received the cd transgene. Results : The antiproliferative effect of AdCMVCD was dependent on both concentation of virus and concentration of 5-flurocytosine . Transformed cells demonstrated a bystander effect wherein SMC expressing cd gene exerted an antiproliferative effect on neighboring cells that did not express cd gene . Conclusion:Theresult demonstrates the potential utility of adenovirus-mediated cd gene transfer for the treatment of restenosis after balloon injury.
文摘在说话人识别研究中,基于身份认证向量(Identity vector,IVEC)的说话人建模方法可以有效地提取说话人信息,是目前处于国际前沿的建模方法.本文对身份认证向量后接支持向量机(Identity vector followed by support vector machine,IVEC-SVM)的说话人识别系统进行了研究,对比了该系统在十种不同核函数下的识别性能,并与文献中身份认证向量后接余弦距离打分(Identity vector followed by cosine distance scoring,IVEC-CDS)系统进行了比较.在美国国家标准技术局(American National Institute of Standards and Technology,NIST)组织的2010年电话信道—电话信道说话人识别核心评测数据库上的实验结果显示,基于核函数的IVEC-SVM系统性能明显优于IVEC-CDS的系统性能.此外,实验结果表明基于Spline核的IVEC-SVM系统可取得最好的识别性能,与IVEC-CDS系统相比,其等错点(Equal error rate,EER)在分数归一化前后分别降低了10%和3%.
