The antifungal protein HAS1 is a new antifungal protein isolated from the genome of Bacillus subtilis HAS,which has a good inhibitory effect on various pathogenic fungi in sugarcane.This study aimed at evaluating the ...The antifungal protein HAS1 is a new antifungal protein isolated from the genome of Bacillus subtilis HAS,which has a good inhibitory effect on various pathogenic fungi in sugarcane.This study aimed at evaluating the immunological transfer reaction of the test sample through repeated skin contact by observing whether the cavy skin repeatedly exposed to purified protein HAS1 suffers from allergic reaction and how strong is the allergic reaction.The results showed that the test group and the vehicle control group exhibited no allergic reaction in the skin immediately and at 24,48 and 72 h and had an mean reaction score and a sensitization rate both of 0,so the results of the naked eye observation were both nonallergenic to cavy skin;and in the positive control group,the cavies were observed to be highly sensitized immediately after removing the drug,mildly sensitized at 24 h,and not sensitized at 48 and 72 h,which meant the skin allergy was alleviated with the observation time.It is suggested that the purified protein HAS1 is negative for cavy skin anaphylaxis test(nonallergenic to cavy skin),which provides an experimental basis for further utilization of the protein and its coding gene.展开更多
<span style="font-family:Verdana;">In order to contribute to the improvement of the knowledge on the</span><span><span><span style="font-family:;" "="">...<span style="font-family:Verdana;">In order to contribute to the improvement of the knowledge on the</span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"> valorization of biochar as a feed additive in livestock, three biochar made from cassava peelings, bean tops and cocoa pods were manufactured. After analyzing their mineral composition, they were incorporated into rations at a rate of 0.8% in order to evaluate their effects on the performance of reproduction and pre-weaning growth in cavy (</span><i><i><span style="font-family:Verdana;">Cavia</span></i><span> <i><span style="font-family:Verdana;">porcellus</span></i><span style="font-family:Verdana;"></span></span></i></span></span><span style="font-family:Verdana;">). The trials were conducted in the Research Unit of Animal Production and Nutrition (URPRONAN) of the University of Dschang, on 96 adult local breed cavy (from the first generation), including 80 females and 16 males aged 4 months and with an average weight of 400 ± 0.25 g. After 2 weeks of adaptation in the rearing boxes at the farm, the females were put in reproduction for a period of 31 days, the animals were divided into 04 batches of 20 females and 4 males. The animals in the control lot were fed a compound feed without biochar (T) while the oth</span><span style="font-family:Verdana;">ers were fed the compound feed containing 0.8% of cassava peel biochar</span><span style="font-family:Verdana;"> (EM), 0.8% of bean tops biochar (FH) and 0.8% of cocoa pod husks biochar (CC) respectively. The results of this study showed that the ash content (26%) and macro-element concentration were higher in the bean husk charcoal compared to the other charcoals and the trace elements were more concentrated in the cassava peel charcoal. The best fertility rate (87.5%) was obtained in </span><span style="font-family:Verdana;">animals receiving the ration containing organic cocoa pod charcoal. The</span><span style="font-family:Verdana;"> highest abortion rate (63.16%) was obtained in the batch of animals receiving the </span><span style="font-family:Verdana;">ration containing organic bean husk charcoal. Pre-weaning mortality rates </span><span style="font-family:Verdana;">were comparable between the different rations. Piglet weights at birth were comparable between the different rations. At weaning, the highest average weight of the kids was recorded with the ration containing the organic bean charcoal (197.25 ± 46.9 g). The highest ADG (4.82 ± 0.74 g/d) and TG (101.25 g) were recorded in the batch of animals receiving the FH ration. Thus, the incorporation of 0.8% of the powder of organic coals seems to have adverse effects on reproductive performance. On the other hand, these coals significantly improve the pre-weaning growth performance in cavy.</span></span>展开更多
In order to increase the productivity of the local cavies as a meat animal, a study was carried out between November 2021 and March 2022 at the Research and Experimental Farm, Animal Nutrition and Production Research ...In order to increase the productivity of the local cavies as a meat animal, a study was carried out between November 2021 and March 2022 at the Research and Experimental Farm, Animal Nutrition and Production Research Unit of the Faculty of Agronomy and Agricultural Sciences (FAAS) and the Microbiology and Anti-Microbial Research Unite of the University of Dschang on 72 cavies aged between 4 and 5 months and weighing an average of 450 ± 50 g produced on the farm, including 60 females and 12 males. The females were distributed according to a completely randomized factorial design in twelve (12) rearing boxes numbered G1 to G12, with 15 females per group distributed in 03 boxes and receiving iso-nitrogenated experimental diet (18% protein) R0 enriched with 20% of Stylosanthes guianensis (0% garlic powder), R0.25 (0.25% garlic powder), R0.50 (0.50% garlic powder) and R0.75 (0.75% garlic powder). Females were identified by numbered ear tags and mated with a sex ratio of 5 females for 1 male. Drinking water enriched with vitamin C (01 tablet of 240 mg in 1.5 liters of water) was served ad libitum and renewed daily. The males were removed after 31 days. A form was established to record the weight of each pregnant female, any abortions during gestation, the date of parturition and the date of birth of each newborn, as well as the number of live-born or stillborn pups. After parturition, the weights of female and newborn cavies were recorded weekly until the 3<sup>rd</sup> week. The number of cavies that died before weaning and the number of live weaned cavies were also recorded. Reproductive performance significantly increased (p 0.05) fertility rate and net fertility rate, litter size, birth viability rate, and pre-weaning viability rate with rations containing garlic powder, compared to the control ration, especially with the R0.50 ration. In addition, birth mortality and pre-weaning mortality were significantly lower (p 0.05) with rations containing the additive, compared to those of the control ration (R0). However, the R0.50 ration showed the lowest rates, followed by the R0.25 ration, and finally the R0.75 ration. Weight development of young cavies increased with all rations but was more marked with the R0.50 ration. Only total weight gain (TG) and average daily weight gain (ADG) in the pre-weaning phase were significantly increased (p < 0.05) with rations R0.25 and R0.50.展开更多
基金Supported by National Natural Science Foundation(31471555)National Key Research and Development Project(SQ2018YFD020024)
文摘The antifungal protein HAS1 is a new antifungal protein isolated from the genome of Bacillus subtilis HAS,which has a good inhibitory effect on various pathogenic fungi in sugarcane.This study aimed at evaluating the immunological transfer reaction of the test sample through repeated skin contact by observing whether the cavy skin repeatedly exposed to purified protein HAS1 suffers from allergic reaction and how strong is the allergic reaction.The results showed that the test group and the vehicle control group exhibited no allergic reaction in the skin immediately and at 24,48 and 72 h and had an mean reaction score and a sensitization rate both of 0,so the results of the naked eye observation were both nonallergenic to cavy skin;and in the positive control group,the cavies were observed to be highly sensitized immediately after removing the drug,mildly sensitized at 24 h,and not sensitized at 48 and 72 h,which meant the skin allergy was alleviated with the observation time.It is suggested that the purified protein HAS1 is negative for cavy skin anaphylaxis test(nonallergenic to cavy skin),which provides an experimental basis for further utilization of the protein and its coding gene.
文摘<span style="font-family:Verdana;">In order to contribute to the improvement of the knowledge on the</span><span><span><span style="font-family:;" "=""><span style="font-family:Verdana;"> valorization of biochar as a feed additive in livestock, three biochar made from cassava peelings, bean tops and cocoa pods were manufactured. After analyzing their mineral composition, they were incorporated into rations at a rate of 0.8% in order to evaluate their effects on the performance of reproduction and pre-weaning growth in cavy (</span><i><i><span style="font-family:Verdana;">Cavia</span></i><span> <i><span style="font-family:Verdana;">porcellus</span></i><span style="font-family:Verdana;"></span></span></i></span></span><span style="font-family:Verdana;">). The trials were conducted in the Research Unit of Animal Production and Nutrition (URPRONAN) of the University of Dschang, on 96 adult local breed cavy (from the first generation), including 80 females and 16 males aged 4 months and with an average weight of 400 ± 0.25 g. After 2 weeks of adaptation in the rearing boxes at the farm, the females were put in reproduction for a period of 31 days, the animals were divided into 04 batches of 20 females and 4 males. The animals in the control lot were fed a compound feed without biochar (T) while the oth</span><span style="font-family:Verdana;">ers were fed the compound feed containing 0.8% of cassava peel biochar</span><span style="font-family:Verdana;"> (EM), 0.8% of bean tops biochar (FH) and 0.8% of cocoa pod husks biochar (CC) respectively. The results of this study showed that the ash content (26%) and macro-element concentration were higher in the bean husk charcoal compared to the other charcoals and the trace elements were more concentrated in the cassava peel charcoal. The best fertility rate (87.5%) was obtained in </span><span style="font-family:Verdana;">animals receiving the ration containing organic cocoa pod charcoal. The</span><span style="font-family:Verdana;"> highest abortion rate (63.16%) was obtained in the batch of animals receiving the </span><span style="font-family:Verdana;">ration containing organic bean husk charcoal. Pre-weaning mortality rates </span><span style="font-family:Verdana;">were comparable between the different rations. Piglet weights at birth were comparable between the different rations. At weaning, the highest average weight of the kids was recorded with the ration containing the organic bean charcoal (197.25 ± 46.9 g). The highest ADG (4.82 ± 0.74 g/d) and TG (101.25 g) were recorded in the batch of animals receiving the FH ration. Thus, the incorporation of 0.8% of the powder of organic coals seems to have adverse effects on reproductive performance. On the other hand, these coals significantly improve the pre-weaning growth performance in cavy.</span></span>
文摘In order to increase the productivity of the local cavies as a meat animal, a study was carried out between November 2021 and March 2022 at the Research and Experimental Farm, Animal Nutrition and Production Research Unit of the Faculty of Agronomy and Agricultural Sciences (FAAS) and the Microbiology and Anti-Microbial Research Unite of the University of Dschang on 72 cavies aged between 4 and 5 months and weighing an average of 450 ± 50 g produced on the farm, including 60 females and 12 males. The females were distributed according to a completely randomized factorial design in twelve (12) rearing boxes numbered G1 to G12, with 15 females per group distributed in 03 boxes and receiving iso-nitrogenated experimental diet (18% protein) R0 enriched with 20% of Stylosanthes guianensis (0% garlic powder), R0.25 (0.25% garlic powder), R0.50 (0.50% garlic powder) and R0.75 (0.75% garlic powder). Females were identified by numbered ear tags and mated with a sex ratio of 5 females for 1 male. Drinking water enriched with vitamin C (01 tablet of 240 mg in 1.5 liters of water) was served ad libitum and renewed daily. The males were removed after 31 days. A form was established to record the weight of each pregnant female, any abortions during gestation, the date of parturition and the date of birth of each newborn, as well as the number of live-born or stillborn pups. After parturition, the weights of female and newborn cavies were recorded weekly until the 3<sup>rd</sup> week. The number of cavies that died before weaning and the number of live weaned cavies were also recorded. Reproductive performance significantly increased (p 0.05) fertility rate and net fertility rate, litter size, birth viability rate, and pre-weaning viability rate with rations containing garlic powder, compared to the control ration, especially with the R0.50 ration. In addition, birth mortality and pre-weaning mortality were significantly lower (p 0.05) with rations containing the additive, compared to those of the control ration (R0). However, the R0.50 ration showed the lowest rates, followed by the R0.25 ration, and finally the R0.75 ration. Weight development of young cavies increased with all rations but was more marked with the R0.50 ration. Only total weight gain (TG) and average daily weight gain (ADG) in the pre-weaning phase were significantly increased (p < 0.05) with rations R0.25 and R0.50.
