Resistance to whitebacked planthopper(WBPH)in Chinese japonica riceChunjiang 06(CJ-06)was mediated by sucking inhibitory and ovicidal mecha-nism.The ovicidal reponse was a common self-defense mechanism againstWBPH in ...Resistance to whitebacked planthopper(WBPH)in Chinese japonica riceChunjiang 06(CJ-06)was mediated by sucking inhibitory and ovicidal mecha-nism.The ovicidal reponse was a common self-defense mechanism againstWBPH in japonica.The ovicidal gene and its chromosomal position had alreadybeen identified.The sucking inhibitory nature of CJ-06 caused a definenon-preference behavior of WBPH in fields.A single dominant gene governed展开更多
Pre-harvest sprouting(PHS) adversely affects wheat quality and yield, and grain color(GC) is associated with PHS resistance. However, the genetic relationship between GC and PHS resistance remains unclear. In this stu...Pre-harvest sprouting(PHS) adversely affects wheat quality and yield, and grain color(GC) is associated with PHS resistance. However, the genetic relationship between GC and PHS resistance remains unclear. In this study, 168 wheat varieties(lines) with significant differences in GC and PHS resistance were genotyped using an Illumina 90K iSelect SNP array. Genome-wide association study(GWAS) based on a mixed linear model showed that 67 marker-trait associations(MTAs) assigned to 29 loci, including 17 potentially novel loci, were significantly associated with GC, which explained 1.1–17.0% of the phenotypic variation. In addition, 100 MTAs belonging to 54 loci, including 31 novel loci, were significantly associated with PHS resistance, which accounted for 1.1–14.7% of the phenotypic variation. Subsequently, two cleaved amplified polymorphic sequences(CAPS) markers, 2B-448 on chromosome 2B and 5B-301 on chromosome 5B,were developed from the representative SNPs of the major common loci Qgc.ahau-2B.3/Qphs.ahau-2B.4controlling GC/PHS resistance and PHS resistance locus Qphs.ahau-5B.4, respectively. Further validation in 171 Chinese mini-core collections confirmed significant correlations of the two CAPS markers with GC and PHS resistance phenotypes under different environments(P<0.05). Furthermore, the wheat public expression database, transcriptomic sequencing, and gene allelic variation analysis identified TraesCS5B02G545100, which encodes glutaredoxin, as a potential candidate gene linked to Qphs.ahau-5B.4. The new CAPS marker CAPS-356 was then developed based on the SNP(T/C) in the coding sequences(CDS) region of TraesCS5B02G545100. The high-density linkage map of the Jing 411/Hongmangchun 21 recombinant inbred lines(RILs) constructed based on specific locus amplified fragment sequencing markers showed that CAPS-356 collocated with a novel QTL for PHS resistance, supporting the role of TraesCS5B02G545100 as the potential candidate gene linked to Qphs.ahau-5B.4. These results provide valuable information for the map-based cloning of Qphs.ahau-5B.4 and breeding of PHS resistant white-grained varieties.展开更多
Fusarium is usually thought to cause soybean root rot, which results in a large quantity of annual yield loss in soybean production, by its secretions including Fusarium toxins and cell wall degrading enzymes, but not...Fusarium is usually thought to cause soybean root rot, which results in a large quantity of annual yield loss in soybean production, by its secretions including Fusarium toxins and cell wall degrading enzymes, but not by the conidia themselves that do not underlie any virulence so far. Here we report that the conidia of one Fusarium solani isolate are able to be virulent to soybean and make soybean seedlings wilted alone. We isolated them from the wilted plants in a soybean-production field and molecularly identified 17 Fusarium isolates through phylogenetic analysis. Of them, except for one isolate that showed diversity of virulence to different soybeans （virulent to one soybean whereas avirulent to another soybean）, the others were all virulent to the two tested soybeans： both conidia cultures and secretions could make soybean seedlings wilted at 5 days post infection, and their virulence had dosage effects that only conidia cultures of at least 5×10^6 conidia mL-1 could show virulence to soybean; however, the sole conidia of the F. solani isolate #4 also exhibited virulence to soybean and could make soybean seedlings wilted. Finally, we developed the specific cleaved amplified polymorphic sequences （CAPS） markers to easily differentiate Fusarium isolates. The isolate #4 in this work will likely be used to investigate the new mechanism of virulence of Fusarium to soybean.展开更多
Lignans are critical bioactive compounds in sesame(Sesamum indicum L.),valued for their health benefits and industrial potential.However,the genetic mechanisms underlying the lignan biosynthesis pathway remain poorly ...Lignans are critical bioactive compounds in sesame(Sesamum indicum L.),valued for their health benefits and industrial potential.However,the genetic mechanisms underlying the lignan biosynthesis pathway remain poorly understood.This study aimed to identify key quantitative trait loci(QTLs) associated with lignan content using 140 F_9 recombinant inbred lines(RILs) derived from a cross between the sesame cultivars Areum and Gomazou,and to develop molecular markers for breeding programs to enhance lignan content.Genotyping-by-sequencing(GBS) technology was employed,combining single(ApeKI) and double-enzyme(Pstl/Mspl) digestions.This approach provided comprehensive genome coverage and substantially improved the quality of the linkage map.A total of 19 QTLs associated with lignan components,including oilsoluble and water-soluble lignans,were identified.Among these,loci on chromosomes 6 and 11 exhibited high LOD scores of 17.8(PVE 13.9%) and 51.5(PVE 68.7%),respectively,for total lignan contents in seeds.Two major QTLs,qLIGNAN6and qLIGNAN11,were identified as key contributors to lignan content.Significant single nucleotide polymorphisms(SNPs)linked to these QTLs were used to develop CAPS markers for potential application in marker-assisted selection(MAS).These markers were validated in RILs and germplasm to evaluate beneficial allele stacking and gene pyramiding.This study provides valuable genetic resources and molecular markers to facilitate the effective implementation of sesame breeding strategies to improve nutritional value.The findings contribute to the development of sesame varieties with higher lignan content,supporting both health and industrial applications.展开更多
文摘Resistance to whitebacked planthopper(WBPH)in Chinese japonica riceChunjiang 06(CJ-06)was mediated by sucking inhibitory and ovicidal mecha-nism.The ovicidal reponse was a common self-defense mechanism againstWBPH in japonica.The ovicidal gene and its chromosomal position had alreadybeen identified.The sucking inhibitory nature of CJ-06 caused a definenon-preference behavior of WBPH in fields.A single dominant gene governed
基金supported by grants from the University Synergy Innovation Program of Anhui Province,China(GXXT-2021-058)the National Natural Science Foundation of China(Joint Fund Projects,U20A2033)+3 种基金the Natural Science Foundation of Anhui Province,China(2108085MC98)the Jiangsu Collaborative Innovation Center for Modern Crop Production,China(JCIC-MCP)the key scientific and technological breakthroughs of Anhui Province(2021d06050003)the joint key project of improved wheat variety of Anhui Province,China(21803003).
文摘Pre-harvest sprouting(PHS) adversely affects wheat quality and yield, and grain color(GC) is associated with PHS resistance. However, the genetic relationship between GC and PHS resistance remains unclear. In this study, 168 wheat varieties(lines) with significant differences in GC and PHS resistance were genotyped using an Illumina 90K iSelect SNP array. Genome-wide association study(GWAS) based on a mixed linear model showed that 67 marker-trait associations(MTAs) assigned to 29 loci, including 17 potentially novel loci, were significantly associated with GC, which explained 1.1–17.0% of the phenotypic variation. In addition, 100 MTAs belonging to 54 loci, including 31 novel loci, were significantly associated with PHS resistance, which accounted for 1.1–14.7% of the phenotypic variation. Subsequently, two cleaved amplified polymorphic sequences(CAPS) markers, 2B-448 on chromosome 2B and 5B-301 on chromosome 5B,were developed from the representative SNPs of the major common loci Qgc.ahau-2B.3/Qphs.ahau-2B.4controlling GC/PHS resistance and PHS resistance locus Qphs.ahau-5B.4, respectively. Further validation in 171 Chinese mini-core collections confirmed significant correlations of the two CAPS markers with GC and PHS resistance phenotypes under different environments(P<0.05). Furthermore, the wheat public expression database, transcriptomic sequencing, and gene allelic variation analysis identified TraesCS5B02G545100, which encodes glutaredoxin, as a potential candidate gene linked to Qphs.ahau-5B.4. The new CAPS marker CAPS-356 was then developed based on the SNP(T/C) in the coding sequences(CDS) region of TraesCS5B02G545100. The high-density linkage map of the Jing 411/Hongmangchun 21 recombinant inbred lines(RILs) constructed based on specific locus amplified fragment sequencing markers showed that CAPS-356 collocated with a novel QTL for PHS resistance, supporting the role of TraesCS5B02G545100 as the potential candidate gene linked to Qphs.ahau-5B.4. These results provide valuable information for the map-based cloning of Qphs.ahau-5B.4 and breeding of PHS resistant white-grained varieties.
基金financially supported by the Innovation Program and Youth Elite Program of Chinese Academy of Agricultural Sciences
文摘Fusarium is usually thought to cause soybean root rot, which results in a large quantity of annual yield loss in soybean production, by its secretions including Fusarium toxins and cell wall degrading enzymes, but not by the conidia themselves that do not underlie any virulence so far. Here we report that the conidia of one Fusarium solani isolate are able to be virulent to soybean and make soybean seedlings wilted alone. We isolated them from the wilted plants in a soybean-production field and molecularly identified 17 Fusarium isolates through phylogenetic analysis. Of them, except for one isolate that showed diversity of virulence to different soybeans （virulent to one soybean whereas avirulent to another soybean）, the others were all virulent to the two tested soybeans： both conidia cultures and secretions could make soybean seedlings wilted at 5 days post infection, and their virulence had dosage effects that only conidia cultures of at least 5×10^6 conidia mL-1 could show virulence to soybean; however, the sole conidia of the F. solani isolate #4 also exhibited virulence to soybean and could make soybean seedlings wilted. Finally, we developed the specific cleaved amplified polymorphic sequences （CAPS） markers to easily differentiate Fusarium isolates. The isolate #4 in this work will likely be used to investigate the new mechanism of virulence of Fusarium to soybean.
基金supported by the Cooperative Research Program for Agriculture Science and Technology Development, Rural Development Administration, Republic of Korea (Project PJ017387072024)supported by the 2024 Collaborative Research Program Between the University and the Rural Development Administration, Republic of Korea。
文摘Lignans are critical bioactive compounds in sesame(Sesamum indicum L.),valued for their health benefits and industrial potential.However,the genetic mechanisms underlying the lignan biosynthesis pathway remain poorly understood.This study aimed to identify key quantitative trait loci(QTLs) associated with lignan content using 140 F_9 recombinant inbred lines(RILs) derived from a cross between the sesame cultivars Areum and Gomazou,and to develop molecular markers for breeding programs to enhance lignan content.Genotyping-by-sequencing(GBS) technology was employed,combining single(ApeKI) and double-enzyme(Pstl/Mspl) digestions.This approach provided comprehensive genome coverage and substantially improved the quality of the linkage map.A total of 19 QTLs associated with lignan components,including oilsoluble and water-soluble lignans,were identified.Among these,loci on chromosomes 6 and 11 exhibited high LOD scores of 17.8(PVE 13.9%) and 51.5(PVE 68.7%),respectively,for total lignan contents in seeds.Two major QTLs,qLIGNAN6and qLIGNAN11,were identified as key contributors to lignan content.Significant single nucleotide polymorphisms(SNPs)linked to these QTLs were used to develop CAPS markers for potential application in marker-assisted selection(MAS).These markers were validated in RILs and germplasm to evaluate beneficial allele stacking and gene pyramiding.This study provides valuable genetic resources and molecular markers to facilitate the effective implementation of sesame breeding strategies to improve nutritional value.The findings contribute to the development of sesame varieties with higher lignan content,supporting both health and industrial applications.
