The dependence of import of chicken heart apocytochrome c on its transformation to holoform by heme attachment was studied. Results showed that there was no difference in the translocation of apocytochrome c across th...The dependence of import of chicken heart apocytochrome c on its transformation to holoform by heme attachment was studied. Results showed that there was no difference in the translocation of apocytochrome c across the mitochondrial membrane in the presence or absence of hemin + dithionite. Furthermore, two heme unattached mutants (H18D, C17S) were prepared, which could still be accumulated in mitochondria, but their import velocity was obviously reduced.展开更多
以羧甲基纤维素钠（CM C）为功能单体,K N O3为模板分子,18-冠醚-6（18-C-6）为致孔剂,去除模板离子（K（C））后制备了钾离子印迹膜层（K-IIM）.以戊二醛（G A）改性壳聚糖（CS）得到阴膜液,将阴膜液流延于上述K-IIM上形成了K（C）离...以羧甲基纤维素钠（CM C）为功能单体,K N O3为模板分子,18-冠醚-6（18-C-6）为致孔剂,去除模板离子（K（C））后制备了钾离子印迹膜层（K-IIM）.以戊二醛（G A）改性壳聚糖（CS）得到阴膜液,将阴膜液流延于上述K-IIM上形成了K（C）离子印迹双极膜（K-IIBPM）.采用扫描电镜（SEM）观察了K-IIBPM的形貌.K-IIM具有沟壑状的孔穴结构,孔穴半径约为0.130~0.170 nm,与K（C）的半径（0.137~0.164 nm）和形状相匹配,且具有规则性;经戊二醛改性后的阴膜层具有不规则的粗糙纹理.采用已循环使用十次的K-IIBPM富集海水中的钾,当海水中N a：K：Ca：M g的质量浓度（g L-1）比为10.70：0.41：0.42：1.32,压力差为0.03 M Pa,渗透时间为60 m in时,K（C）的透过率高达96%,其它被分离离子的截留率均在85%以上,从而实现富集海水中钾的目的.展开更多
基金Project supported by the National Natural Science Foundation of China (Grant No. 39730130).
文摘The dependence of import of chicken heart apocytochrome c on its transformation to holoform by heme attachment was studied. Results showed that there was no difference in the translocation of apocytochrome c across the mitochondrial membrane in the presence or absence of hemin + dithionite. Furthermore, two heme unattached mutants (H18D, C17S) were prepared, which could still be accumulated in mitochondria, but their import velocity was obviously reduced.
文摘以羧甲基纤维素钠（CM C）为功能单体,K N O3为模板分子,18-冠醚-6（18-C-6）为致孔剂,去除模板离子（K（C））后制备了钾离子印迹膜层（K-IIM）.以戊二醛（G A）改性壳聚糖（CS）得到阴膜液,将阴膜液流延于上述K-IIM上形成了K（C）离子印迹双极膜（K-IIBPM）.采用扫描电镜（SEM）观察了K-IIBPM的形貌.K-IIM具有沟壑状的孔穴结构,孔穴半径约为0.130~0.170 nm,与K（C）的半径（0.137~0.164 nm）和形状相匹配,且具有规则性;经戊二醛改性后的阴膜层具有不规则的粗糙纹理.采用已循环使用十次的K-IIBPM富集海水中的钾,当海水中N a：K：Ca：M g的质量浓度（g L-1）比为10.70：0.41：0.42：1.32,压力差为0.03 M Pa,渗透时间为60 m in时,K（C）的透过率高达96%,其它被分离离子的截留率均在85%以上,从而实现富集海水中钾的目的.
