Different from traditional aggregation method, the unreliable buffers are originally considered and a more general aggregation method is offered, in which not only the unreliable buffers are considered, but also the p...Different from traditional aggregation method, the unreliable buffers are originally considered and a more general aggregation method is offered, in which not only the unreliable buffers are considered, but also the probabilities of system states are obtained by a discrete model rather than the continuous flow model of unreliable manufacturing systems. The solution technique is offered to get the system sate probabilities. The method advances the traditional system aggregation techniques. Numerical results specify the extended aggregation method and also show that the unreliable limited buffers have a strong impact on the efficiency of the production lines.展开更多
[Objective] This study aimed to investigate a reliable method for DNA ex- traction from Wusuli raccoon dog＇s hair. [Method] Several DNA extraction methods were used to extract DNA from Wusuli raccoon dog hair, includ...[Objective] This study aimed to investigate a reliable method for DNA ex- traction from Wusuli raccoon dog＇s hair. [Method] Several DNA extraction methods were used to extract DNA from Wusuli raccoon dog hair, including Chelex-100 method, PCR buffer method, organic phenol-chloroform method and centrifugal col- umn type kit method. The extracted DNA was analyzed by using PCR amplification and electrophoresis to compare these four DNA extraction methods. [Result] Accord- ing to the results of spectrophotometer detection and gel electrophoresis, nucleic acid extracted by Chetex-100 method had proteins and other impurities; nucleic acid ex- tracted by PCR buffer method was low in concentration; however, DNA extracted by organic phenol-chloroform method and centrifugal column type kit was high in con- centration with no impurity band. [Conclusion] This study had laid the strong founda- tion of scientific theory to further explore the efficient and simple method for extracting DNA from Wusuli raccoon dog hair follicle.展开更多
Aimed at the difficulties in analyzing the buffer characteristics of airbag system by using thermodynamic or experimental method only,the finite element method was used to establish nonlinear models for heavy equipmen...Aimed at the difficulties in analyzing the buffer characteristics of airbag system by using thermodynamic or experimental method only,the finite element method was used to establish nonlinear models for heavy equipment and its airbag system.The models' efficiency and correctness were validated by using on-site experiment data in vehicle airdrop landing.The simulation results agree very well with the experiment results.Then,the environment adaptability of airbag system of heavy equipment under high-altitude condition was studied by using the models.Finally,some solutions were given to solve the overturn problem in the landing.展开更多
基金Great Technology Innovation of Gansu Province,China (No.2GS063-A52-005-01)Natural Science Foundation of Gansu Province,China (No.3ZS062-B25-034)Research Item of Education Department of Gansu Province,China (No.0703-06)
文摘Different from traditional aggregation method, the unreliable buffers are originally considered and a more general aggregation method is offered, in which not only the unreliable buffers are considered, but also the probabilities of system states are obtained by a discrete model rather than the continuous flow model of unreliable manufacturing systems. The solution technique is offered to get the system sate probabilities. The method advances the traditional system aggregation techniques. Numerical results specify the extended aggregation method and also show that the unreliable limited buffers have a strong impact on the efficiency of the production lines.
基金Supported by National Natural Science Foundation of China (31072018)~~
文摘[Objective] This study aimed to investigate a reliable method for DNA ex- traction from Wusuli raccoon dog＇s hair. [Method] Several DNA extraction methods were used to extract DNA from Wusuli raccoon dog hair, including Chelex-100 method, PCR buffer method, organic phenol-chloroform method and centrifugal col- umn type kit method. The extracted DNA was analyzed by using PCR amplification and electrophoresis to compare these four DNA extraction methods. [Result] Accord- ing to the results of spectrophotometer detection and gel electrophoresis, nucleic acid extracted by Chetex-100 method had proteins and other impurities; nucleic acid ex- tracted by PCR buffer method was low in concentration; however, DNA extracted by organic phenol-chloroform method and centrifugal column type kit was high in con- centration with no impurity band. [Conclusion] This study had laid the strong founda- tion of scientific theory to further explore the efficient and simple method for extracting DNA from Wusuli raccoon dog hair follicle.
文摘Aimed at the difficulties in analyzing the buffer characteristics of airbag system by using thermodynamic or experimental method only,the finite element method was used to establish nonlinear models for heavy equipment and its airbag system.The models' efficiency and correctness were validated by using on-site experiment data in vehicle airdrop landing.The simulation results agree very well with the experiment results.Then,the environment adaptability of airbag system of heavy equipment under high-altitude condition was studied by using the models.Finally,some solutions were given to solve the overturn problem in the landing.
