Background:Pyogenic granuloma(PG)is a benign vascular skin lesion that occurs in children.Although,sclerotherapy is a common treatment for patients with PG,all the previous studies have been case reports or series.At ...Background:Pyogenic granuloma(PG)is a benign vascular skin lesion that occurs in children.Although,sclerotherapy is a common treatment for patients with PG,all the previous studies have been case reports or series.At present,no reports have compared the efficacy of the two different sclerosing agents,polidocanol and bleomycin,in the treatment of PG.Therefore,we aimed to compare the cure rates and adverse reactions associated with these two agents in sclerotherapy for PG in children.Methods:This retrospective analysis included children<18 years of age with PG undergoing cutaneous treatment at our hospital between January 2016 and January 2022.Two sclerosing agents,polidocanol and bleomycin,were topically injected.The efficacy and incidence of adverse reactions were compared between the two groups.Results:A total of 117 children with PG were divided into the polidocanol(n=52)and bleomycin(n=65)groups.Lesions disappeared after one injection in 38 children,two in 11 children,and three in 3 children in the polidocanol group.A similar phenomenon was observed after one injection in 53 children,two injections in 8 children,and three injections in children in the bleomycin group.The single-injection cure rate was not significantly different between the two groups(P>0.05).The rate of adverse reactions was significantly different between the two groups(P<0.05).No severe complications occurred,and no recurrences were detected during the 6-12 months of postoperative follow-up period.Conclusion:This study showed that both polidocanol and bleomycin are safe and effective sclerosing agents for treatment of PG in children.The incidence of adverse reactions to polidocanol was lower than that to bleomycin.We recommend sclerotherapy with polidocanol as a first-line treatment for PG,as it is suitable for application in hospitals at various levels.展开更多
Objective:To evaluate the effect of trigonelline on bleomycin-induced idiopathic pulmonary fibrosis(IPF)and to explore its underlying mechanisms using network pharmacology.Methods:IPF was induced in C57BL/6 mice by a ...Objective:To evaluate the effect of trigonelline on bleomycin-induced idiopathic pulmonary fibrosis(IPF)and to explore its underlying mechanisms using network pharmacology.Methods:IPF was induced in C57BL/6 mice by a single intratracheal instillation of bleomycin(5 mg/kg).Trigonelline was administered at doses of 25,50,and 100 mg/kg/day orally from the 2nd day post-bleomycin induction up to the 14th day.In IPF-induced mice,lung coefficient,immune cell infiltration in bronchoalveolar lavage fluid,and oxidative stress were measured.Histological alterations in lung tissues were also assessed.Moreover,network pharmacology approach was conducted to reveal molecular interactions of bleomycin and trigonelline with targets of IPF.Results:Trigonelline treatment reduced bleomycin-induced oxidative stress and immune cell infiltration,and mitigated physiological changes in the lung tissues of mice.Moreover,trigonelline alleviated bleomycin-induced histological alterations in lung tissues.Network pharmacology analysis showed that bleomycin and trigonelline interacted with IPF targets,such as NFKB1,HDAC2,HIF1A,and TLR4.Conclusions:The interaction of trigonelline with key IPF targets and its ameliorative effects on lung damage and oxidative stress highlight its potential in treating IPF.It may be considered an antifibrotic agent for further clinical development.展开更多
Background:To explore the effects and mechanisms of Bu-Yang-Huan-Wu Decoction on pulmonary fibrosis in mice.Methods:Forty-five C57BL/6J mice were randomly divided into three groups:Control,Model,and Bu-Yang-Huan-Wu De...Background:To explore the effects and mechanisms of Bu-Yang-Huan-Wu Decoction on pulmonary fibrosis in mice.Methods:Forty-five C57BL/6J mice were randomly divided into three groups:Control,Model,and Bu-Yang-Huan-Wu Decoction.Pulmonary fibrosis was elicited in mice through a solitary intratracheal administration of 2.5 mg/kg bleomycin.For the control group,mice were given a solitary intratracheal administration of a comparable volume of PBS.Treatment began on the first day after the successful model establishment and lasted for 21 days.The survival rate and body weight of the mice were recorded daily,and on the 22nd day,bronchoalveolar lavage fluid was collected to determine total cells and total protein.The wet/dry weight ratio of lung tissue and hydroxyproline were measured.Lung tissue pathology was observed using hematoxylin and eosin staining and Masson staining.The mRNA expression of epithelial-mesenchymal transition-related proteins(E-cadherin and vimentin)was detected by RT-qPCR,and their protein expression was analyzed by western blot.Results:Compared to the model group,the Bu-Yang-Huan-Wu Decoction treatment notably enhanced both the survival rate and body weight in pulmonary fibrosis mice,significantly reduced lung tissue wet/dry weight ratio,total cells,and protein in bronchoalveolar lavage fluid,and hydroxyproline content.The pathological morphology of lung tissue was significantly improved,with increased expression of the epithelial cell marker E-cadherin mRNA and protein,and decreased expression of the mesenchymal cell marker vimentin mRNA and protein.Conclusion:Bu-Yang-Huan-Wu Decoction can improve the degree of bleomycin-induced pulmonary fibrosis in mice by inhibiting epithelial-mesenchymal transition.展开更多
文摘Background:Pyogenic granuloma(PG)is a benign vascular skin lesion that occurs in children.Although,sclerotherapy is a common treatment for patients with PG,all the previous studies have been case reports or series.At present,no reports have compared the efficacy of the two different sclerosing agents,polidocanol and bleomycin,in the treatment of PG.Therefore,we aimed to compare the cure rates and adverse reactions associated with these two agents in sclerotherapy for PG in children.Methods:This retrospective analysis included children<18 years of age with PG undergoing cutaneous treatment at our hospital between January 2016 and January 2022.Two sclerosing agents,polidocanol and bleomycin,were topically injected.The efficacy and incidence of adverse reactions were compared between the two groups.Results:A total of 117 children with PG were divided into the polidocanol(n=52)and bleomycin(n=65)groups.Lesions disappeared after one injection in 38 children,two in 11 children,and three in 3 children in the polidocanol group.A similar phenomenon was observed after one injection in 53 children,two injections in 8 children,and three injections in children in the bleomycin group.The single-injection cure rate was not significantly different between the two groups(P>0.05).The rate of adverse reactions was significantly different between the two groups(P<0.05).No severe complications occurred,and no recurrences were detected during the 6-12 months of postoperative follow-up period.Conclusion:This study showed that both polidocanol and bleomycin are safe and effective sclerosing agents for treatment of PG in children.The incidence of adverse reactions to polidocanol was lower than that to bleomycin.We recommend sclerotherapy with polidocanol as a first-line treatment for PG,as it is suitable for application in hospitals at various levels.
文摘Objective:To evaluate the effect of trigonelline on bleomycin-induced idiopathic pulmonary fibrosis(IPF)and to explore its underlying mechanisms using network pharmacology.Methods:IPF was induced in C57BL/6 mice by a single intratracheal instillation of bleomycin(5 mg/kg).Trigonelline was administered at doses of 25,50,and 100 mg/kg/day orally from the 2nd day post-bleomycin induction up to the 14th day.In IPF-induced mice,lung coefficient,immune cell infiltration in bronchoalveolar lavage fluid,and oxidative stress were measured.Histological alterations in lung tissues were also assessed.Moreover,network pharmacology approach was conducted to reveal molecular interactions of bleomycin and trigonelline with targets of IPF.Results:Trigonelline treatment reduced bleomycin-induced oxidative stress and immune cell infiltration,and mitigated physiological changes in the lung tissues of mice.Moreover,trigonelline alleviated bleomycin-induced histological alterations in lung tissues.Network pharmacology analysis showed that bleomycin and trigonelline interacted with IPF targets,such as NFKB1,HDAC2,HIF1A,and TLR4.Conclusions:The interaction of trigonelline with key IPF targets and its ameliorative effects on lung damage and oxidative stress highlight its potential in treating IPF.It may be considered an antifibrotic agent for further clinical development.
文摘Background:To explore the effects and mechanisms of Bu-Yang-Huan-Wu Decoction on pulmonary fibrosis in mice.Methods:Forty-five C57BL/6J mice were randomly divided into three groups:Control,Model,and Bu-Yang-Huan-Wu Decoction.Pulmonary fibrosis was elicited in mice through a solitary intratracheal administration of 2.5 mg/kg bleomycin.For the control group,mice were given a solitary intratracheal administration of a comparable volume of PBS.Treatment began on the first day after the successful model establishment and lasted for 21 days.The survival rate and body weight of the mice were recorded daily,and on the 22nd day,bronchoalveolar lavage fluid was collected to determine total cells and total protein.The wet/dry weight ratio of lung tissue and hydroxyproline were measured.Lung tissue pathology was observed using hematoxylin and eosin staining and Masson staining.The mRNA expression of epithelial-mesenchymal transition-related proteins(E-cadherin and vimentin)was detected by RT-qPCR,and their protein expression was analyzed by western blot.Results:Compared to the model group,the Bu-Yang-Huan-Wu Decoction treatment notably enhanced both the survival rate and body weight in pulmonary fibrosis mice,significantly reduced lung tissue wet/dry weight ratio,total cells,and protein in bronchoalveolar lavage fluid,and hydroxyproline content.The pathological morphology of lung tissue was significantly improved,with increased expression of the epithelial cell marker E-cadherin mRNA and protein,and decreased expression of the mesenchymal cell marker vimentin mRNA and protein.Conclusion:Bu-Yang-Huan-Wu Decoction can improve the degree of bleomycin-induced pulmonary fibrosis in mice by inhibiting epithelial-mesenchymal transition.
