Anthranilic diamides are fasting growing class insecticides in modern crop protection for their high activity, low ecotoxicity, and broad insecticidal spectra. However. the bioactive conformations of anthranilic diami...Anthranilic diamides are fasting growing class insecticides in modern crop protection for their high activity, low ecotoxicity, and broad insecticidal spectra. However. the bioactive conformations of anthranilic diamides are still unclear until now. In the present study, DFT-based potential energy surface scanning was used to detect the low energy conformations of chlorantraniliprole, then were used respectively in the structure alignment for a series of anthranilic diamide compounds followed by detailed CoMFA and CoMSIA analyses. Finally, the bioactive conformations of anthranilic diamide insecticides were revealed from a series of low energy conformations, which might provide some clues for future insecticide design.展开更多
In vivo,vascular endothelial growth factor(VEGF)and vascular endothelial cadherin(VE-cadherin)co-regulate the dynamic organization of endothelial cells during vascular sprouting,balancing angiogenesis and vascular sta...In vivo,vascular endothelial growth factor(VEGF)and vascular endothelial cadherin(VE-cadherin)co-regulate the dynamic organization of endothelial cells during vascular sprouting,balancing angiogenesis and vascular stability.In this study,a novel bioactive surface integrating human VE-cadherin-Fc and VEGF-Fc fusion proteins was innovatively developed for the modification of poly(ε-caprolactone)(PCL)small-caliber electrospun fibrous grafts(VE-cad/VEGF-PCL)to promote the regeneration of functional endothelium and improve the patency of artificial vascular grafts.These fusion proteins self-assembled on the PCL fibers through the hydrophobic binding of Fc domains,improving surface hydrophilicity while reducing the adhesion of fibrinogen.In vitro results showed that the VE-cadherin/VEGF surface upregulated the expression of endogenous VE-cadherin and synergistically activated the VE-cadherin/VEGFR2/FAK/AKT/ERK signal transduction,which facilitated the functioning of human umbilical vein endothelial cells(HUVECs).Moreover,the VE-cadherin/VEGF surface significantly enhanced cellularization and capillary formation,then subsequently accelerated the regeneration of functional endothelium and smooth muscle in the VE-cad/VEGF-PCL grafts in a rat abdominal aorta replacement model.Together,these results highlight the advantages of VE-cadherin/VEGF surface in enhancing rapid endothelialization of electrospun vascular grafts and provide new insights into the design of cross-activating biomaterials.展开更多
This study aimed to investigate the activity of lactase immobilized polycaprolactone and silk fibroin(PCL/SF)-based nanofiber and nitrocellulose membrane for the preparation of lactose-reduced milk.PCL/SF-based nanofi...This study aimed to investigate the activity of lactase immobilized polycaprolactone and silk fibroin(PCL/SF)-based nanofiber and nitrocellulose membrane for the preparation of lactose-reduced milk.PCL/SF-based nanofiber was prepared by using the electrospinning method.The lactase enzyme was immobilized using the physical adsorption method on both surfaces,and then the optimum operating temperature and pH of the immobilized enzymes were determined.The efficiency of the immobilized lactase enzyme was determined in both goat and cow milk.The nutrient content of milk was also analyzed before and after the incubation of nanofiber and membrane with milk.The lactose hydrolysis efficiency of the lactase immobilized nitrocellulose membrane was found to be higher than the lactase immobilized PCL/SF-based nanofiber.Lactose was hydrolyzed 59%in cow milk and 87%in goat milk by using lactase immobilized nitrocellulose membrane.42%of lactose was also hydrolyzed in cow milk and 21%was hydrolyzed in goat milk by using lactase immobilized PCL/SF-based nanofiber.However,the use of these two bioactive surfaces did not change the fat and protein composition of both cow and goat milk.In conclusion,lactase immobilized nitrocellulose membrane was found to be more advantageous in the production of lactose-reduced milk than the lactase immobilized polycaprolactone/silk fibroin nanofiber.展开更多
基金the financial support from the National Key Technology R&D Program of China (No.2011BAE06B05)
文摘Anthranilic diamides are fasting growing class insecticides in modern crop protection for their high activity, low ecotoxicity, and broad insecticidal spectra. However. the bioactive conformations of anthranilic diamides are still unclear until now. In the present study, DFT-based potential energy surface scanning was used to detect the low energy conformations of chlorantraniliprole, then were used respectively in the structure alignment for a series of anthranilic diamide compounds followed by detailed CoMFA and CoMSIA analyses. Finally, the bioactive conformations of anthranilic diamide insecticides were revealed from a series of low energy conformations, which might provide some clues for future insecticide design.
基金The authors gratefully acknowledge financial support from the National Key R&D Program of China(2020YFA0710802)the National Natural Science Foundation of China(Grant No.32071364,82172106)+2 种基金the NCC Fund(NCC2020PY18)Tianjin“Project+Team”Key Training Foundation(XC202035)China Postdoctoral Science Foundation(2022M711707).
文摘In vivo,vascular endothelial growth factor(VEGF)and vascular endothelial cadherin(VE-cadherin)co-regulate the dynamic organization of endothelial cells during vascular sprouting,balancing angiogenesis and vascular stability.In this study,a novel bioactive surface integrating human VE-cadherin-Fc and VEGF-Fc fusion proteins was innovatively developed for the modification of poly(ε-caprolactone)(PCL)small-caliber electrospun fibrous grafts(VE-cad/VEGF-PCL)to promote the regeneration of functional endothelium and improve the patency of artificial vascular grafts.These fusion proteins self-assembled on the PCL fibers through the hydrophobic binding of Fc domains,improving surface hydrophilicity while reducing the adhesion of fibrinogen.In vitro results showed that the VE-cadherin/VEGF surface upregulated the expression of endogenous VE-cadherin and synergistically activated the VE-cadherin/VEGFR2/FAK/AKT/ERK signal transduction,which facilitated the functioning of human umbilical vein endothelial cells(HUVECs).Moreover,the VE-cadherin/VEGF surface significantly enhanced cellularization and capillary formation,then subsequently accelerated the regeneration of functional endothelium and smooth muscle in the VE-cad/VEGF-PCL grafts in a rat abdominal aorta replacement model.Together,these results highlight the advantages of VE-cadherin/VEGF surface in enhancing rapid endothelialization of electrospun vascular grafts and provide new insights into the design of cross-activating biomaterials.
基金Marmara University Scientific Research Project(TYL-2021-10180)units.
文摘This study aimed to investigate the activity of lactase immobilized polycaprolactone and silk fibroin(PCL/SF)-based nanofiber and nitrocellulose membrane for the preparation of lactose-reduced milk.PCL/SF-based nanofiber was prepared by using the electrospinning method.The lactase enzyme was immobilized using the physical adsorption method on both surfaces,and then the optimum operating temperature and pH of the immobilized enzymes were determined.The efficiency of the immobilized lactase enzyme was determined in both goat and cow milk.The nutrient content of milk was also analyzed before and after the incubation of nanofiber and membrane with milk.The lactose hydrolysis efficiency of the lactase immobilized nitrocellulose membrane was found to be higher than the lactase immobilized PCL/SF-based nanofiber.Lactose was hydrolyzed 59%in cow milk and 87%in goat milk by using lactase immobilized nitrocellulose membrane.42%of lactose was also hydrolyzed in cow milk and 21%was hydrolyzed in goat milk by using lactase immobilized PCL/SF-based nanofiber.However,the use of these two bioactive surfaces did not change the fat and protein composition of both cow and goat milk.In conclusion,lactase immobilized nitrocellulose membrane was found to be more advantageous in the production of lactose-reduced milk than the lactase immobilized polycaprolactone/silk fibroin nanofiber.
