Low hip bone mineral density(BMD)is an important index for osteoporosis and is associated with hip fracture,which leads to more cases of disability and mortality than all other kinds of fractures(Kanis et al.,2007).BM...Low hip bone mineral density(BMD)is an important index for osteoporosis and is associated with hip fracture,which leads to more cases of disability and mortality than all other kinds of fractures(Kanis et al.,2007).BMD’s heritability is more than 60%(Arden et al.,1996).A number of candidate loci for BMD have been previously identified by Genome Wide Association Studies(GWAS)(Xiong et al.,2009;Karasik et al.,2010;Zhang et al.,2013).Nevertheless,many significant signals based on GWAS are展开更多
Coumarate 3-hydroxylase(C3h)genes participate in the synthesis of lignin and may affect the properties of wood that are important for its commercial value.A better understanding of the natural variation in C3h genes a...Coumarate 3-hydroxylase(C3h)genes participate in the synthesis of lignin and may affect the properties of wood that are important for its commercial value.A better understanding of the natural variation in C3h genes and their associations to wood properties is required to effectively improve wood quality.We used a candidate gene-based association mapping approach to identify CfC3h allelic variants associated with traits that affect the wood properties of Catalpa fargesii.We first isolated the full-length CfC3h cDNA(1825 bp),which was expressed at relatively high levels in xylem according to real time-polymerase chain reaction.In totally,17 common single-nucleotide polymorphisms(minor allele frequency>5%)were identified through cloning and sequencing the CfC3h locus from a mapping population(including 88 unrelated natural C.fargesii individuals collected from main distribution area).Nucleotide diversity and linkage disequilibrium(LD)in CfC3h indicate that CfC3h has low nucleotide diversity(π_(t)=0.0031 andθ_(w)=0.0103)and relatively low LD(within 1800 bp;r^(2)≥0.1).An association analysis identified eight common single-nucleotide polymorphisms(SNPs)(false discovery rate,Q<0.10)and ten haplotypes(Q<0.10)associated with wood properties,explaining 4.92-12.09%of the phenotypic variance in an association population consisted of 125 unrelated natural individuals(The 88 individuals from the mapping population were comprised in the association population).Our study would provide new insight into C3h gene affecting wood quality,and the SNP markers identified would have potential applications in marker-assisted breeding in the future.展开更多
To the Editor:Common respiratory diseases such as chronic obstructive pulmonary disease(COPD)and asthma induce significant health burdens worldwide.[1,2]Airway/lung microbiome and microbial metabolites have been prove...To the Editor:Common respiratory diseases such as chronic obstructive pulmonary disease(COPD)and asthma induce significant health burdens worldwide.[1,2]Airway/lung microbiome and microbial metabolites have been proven to be closely related to multiple respiratory diseases.[3]However,few relevant studies show characteristics and horizontal comparisons of metabolites of dominant bacteria in respiratory diseases.Additionally,most results are based on association analyses of microbiome sequences,especially those of the gut microbiome,and untargeted metabolomics,without evidence that the metabolites are directly produced and secreted by certain bacteria.[4]In this context,our study aimed to analyze and compare the key metabolites and metabolic pathways of common bacteria involved in respiratory diseases.展开更多
基金partially supported by or benefited from grants from NIH (P50AR055081,R01AG026564,R01AR050496,R01AR059781,D43TW009107,P20GM109036,R01GM109068,R01MH104680,R01MH107354,R01AR057049,and R03TW008221)
文摘Low hip bone mineral density(BMD)is an important index for osteoporosis and is associated with hip fracture,which leads to more cases of disability and mortality than all other kinds of fractures(Kanis et al.,2007).BMD’s heritability is more than 60%(Arden et al.,1996).A number of candidate loci for BMD have been previously identified by Genome Wide Association Studies(GWAS)(Xiong et al.,2009;Karasik et al.,2010;Zhang et al.,2013).Nevertheless,many significant signals based on GWAS are
基金This work was supported by the National Key Research and Development Program of China[No.2017YFD0600201]Forestry Industry Research Special Funds for Public Welfare Projects[No.201404101].
文摘Coumarate 3-hydroxylase(C3h)genes participate in the synthesis of lignin and may affect the properties of wood that are important for its commercial value.A better understanding of the natural variation in C3h genes and their associations to wood properties is required to effectively improve wood quality.We used a candidate gene-based association mapping approach to identify CfC3h allelic variants associated with traits that affect the wood properties of Catalpa fargesii.We first isolated the full-length CfC3h cDNA(1825 bp),which was expressed at relatively high levels in xylem according to real time-polymerase chain reaction.In totally,17 common single-nucleotide polymorphisms(minor allele frequency>5%)were identified through cloning and sequencing the CfC3h locus from a mapping population(including 88 unrelated natural C.fargesii individuals collected from main distribution area).Nucleotide diversity and linkage disequilibrium(LD)in CfC3h indicate that CfC3h has low nucleotide diversity(π_(t)=0.0031 andθ_(w)=0.0103)and relatively low LD(within 1800 bp;r^(2)≥0.1).An association analysis identified eight common single-nucleotide polymorphisms(SNPs)(false discovery rate,Q<0.10)and ten haplotypes(Q<0.10)associated with wood properties,explaining 4.92-12.09%of the phenotypic variance in an association population consisted of 125 unrelated natural individuals(The 88 individuals from the mapping population were comprised in the association population).Our study would provide new insight into C3h gene affecting wood quality,and the SNP markers identified would have potential applications in marker-assisted breeding in the future.
基金supported by grants from the National Natural Science Foundation of China(Nos.82090013,82071805,and 82241011)Beijing Natural Science Foundation(Nos.7212001,7242010,and 7242002).
文摘To the Editor:Common respiratory diseases such as chronic obstructive pulmonary disease(COPD)and asthma induce significant health burdens worldwide.[1,2]Airway/lung microbiome and microbial metabolites have been proven to be closely related to multiple respiratory diseases.[3]However,few relevant studies show characteristics and horizontal comparisons of metabolites of dominant bacteria in respiratory diseases.Additionally,most results are based on association analyses of microbiome sequences,especially those of the gut microbiome,and untargeted metabolomics,without evidence that the metabolites are directly produced and secreted by certain bacteria.[4]In this context,our study aimed to analyze and compare the key metabolites and metabolic pathways of common bacteria involved in respiratory diseases.
