Objective The SOD, POD and APX enzyme activities of tetraploid lines and diploid control line of Chrysanthemum cinerariifolium in test-tube and field were determined in order to provide a reference for the selective b...Objective The SOD, POD and APX enzyme activities of tetraploid lines and diploid control line of Chrysanthemum cinerariifolium in test-tube and field were determined in order to provide a reference for the selective breeding of high resistance good tetraploid varieties of C. cinerariifolium. Method The SOD, POD and APX activities of each sample were determined. Every material should have 3 replicates which been determined and calculated mean value to analyze. Result The various kinds of antioxidase activities in protective enzyme system of tetraploid lines were increased generally in comparison with that of diploid control line. And there is certain relativity between the samples in field and in test-tube of these lines. Conclusion The antioxidase activities of each line in test-tube could be considered as reference index to screen high resistance good tetraploid varieties of C. cinerariifolium.展开更多
[Objective] This study was conducted to determine the physiological and biochemical characteristics of potato tubers infected by Fusarium trichothecioides. [Method] The soluble protein content, SOD activity, POD activ...[Objective] This study was conducted to determine the physiological and biochemical characteristics of potato tubers infected by Fusarium trichothecioides. [Method] The soluble protein content, SOD activity, POD activity and malondialdehyde （MDA） content of the tubers of two potato varieties Atlantic and Keshan 1 infected by F. trichothecioides were measured 0, 3, 6, 9, 12, 24 and 48 h later. [Result] Com- pared with the control uninfected, the contents of soluble protein and MDA in the tu- bers of both potato varieties infected with F. trichothecioides decreased, while the SOD activity increased, indicating that SOD activity had a certain relationship with the resistance of potato to dry rot. The POD activity in Keshan 1 which is resistant to F. trichothecioides was higher than in Atlantic, which is susceptible to F. trichothe- cioides, indicating that POD activity was closely related with potato＇s resistance to Fusarium dry rot. [Conclusion] The physiological and biochemical characteristics of potato tubers infected by F. trichothecioides will provide some references for the early waming and prevention of Fusariurn dry rot.展开更多
The regulatory mechanism of soil cadmium （Cd） on antioxidases in sorghum was studied using 3 sorghum species viz., sweet sorghum [Sorghum bicolor （L.）] Moench. cv. Hunnigreen, sorghum hybrid sudangrass （Sorghum b...The regulatory mechanism of soil cadmium （Cd） on antioxidases in sorghum was studied using 3 sorghum species viz., sweet sorghum [Sorghum bicolor （L.）] Moench. cv. Hunnigreen, sorghum hybrid sudangrass （Sorghum bicolor × S. sudanense, cv. Everlush） and sudangrass [Sorghum sudanense （Piper） Stapf cv. Xinjiang]. The results showed that low concentrations of Cd^2＋ （410 mg kg-0 induced the activities of 4 antioxidases in leaves of the 3 species of sorghum, but high Cd^2＋ concentrations （≥ 50 mg kg-0 significantly reduced the activities of all the four antioxidases. The glutathione （GSH） contents in leaves of the 3 species of sorghum correlated positively with the Cd^2＋ concentrations. The maximum antioxidase activities were observed at the elongation stage of sorghum during the whole growth period.展开更多
With the potato variety Xishu 1 as the test materials, this paper adopted tissue culture to study the effect of Pb and Cd stress with different mass fractions on potato growth and the antioxidase system. The results s...With the potato variety Xishu 1 as the test materials, this paper adopted tissue culture to study the effect of Pb and Cd stress with different mass fractions on potato growth and the antioxidase system. The results showed that under the stress of Pb （200 mg/kg） and Cd （20 mg/kg）, growth of potato had no obvious difference with that in control group; under the stress of Pb （ 〉200 mg/kg） and Cd （ 〉20 mg/kg）, potato growth suspended, and its physiological indexes such as dry weight, plant height, root number, root length were inhibited obviously. With the increasing mass fraction of Pb, relative electrical conductivity, MDA and proline of potato leaves showed the tendency of increase, and the results in high mass fraction treatments showed significant difference with those in the control group （P 〈 0.05 ）, CAT, POD and SOD activity of leaves increased greatly with the increasing mass fraction. In the Pb stress treatment （200 mg/kg）, relative electrical conductivity, MDA and proline of potato leaves showed no obvious difference with those in the control group, and the results in high mass fraction treatments were significantly higher than those in the control group （ P 〈 O. 05 ）, CAT and SOD activity increased greatly with the increasing mass fraction （ P 〈 0.05 ）. Under the stress of Pb and Cd, synthesis of soluble protein in potato leaves was strongly inhibited. The results showed that potato was tolerant to Pb and Cd of lower mass fraction, and damage of potato caused by Pb and Cd stress could be relieved by improving activity of antioxidase and reducing peroxidation of membrane lipid, and the tolerance reduced with the increase of stress.展开更多
To investigate the effects of ultraviolet blood irradiation and oxygenation (UBIO) on free radicals and antioxidase after spinal cord injury in rabbits Methods Totally, 186 rabbits were used and divided randomly in...To investigate the effects of ultraviolet blood irradiation and oxygenation (UBIO) on free radicals and antioxidase after spinal cord injury in rabbits Methods Totally, 186 rabbits were used and divided randomly into four experimental groups: control (n=6), blood transfusion (n=24), injured (n=96) and treatment (n=60) groups The relative intensity of free radical (FR) signals, malondialdehyde (MDA) content, as well as the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH PX) were compared among the four groups at 6, 24, 48, and 72 hours and 6 days after injury Results The relative intensity of FR signals in spinal cord tissue in the injured group increased at 48 hours and showed a striking difference compared with the control group; in the treatment group, it decreased and showed a striking difference compared with the injured group MDA content in blood in the injured group increased and showed a striking difference at 6, 24 and 48 hours and showed a significant difference at 72 hours and 6 days after injury compared with the control group In the treatment group, MDA content in blood decreased and showed a significant difference at 48 hours compared with the injured group MDA content in spinal cord tissue increased in the injured group and showed a striking difference compared with the control group; in the treatment group, it decreased and showed a striking difference compared with the injured group at the corresponding times The activity of SOD in blood and spinal cord tissue decreased in the injured group and showed a striking difference compared with the control group; in the treatment group, it increased and showed a striking difference compared with the injured group at the corresponding times The changes in activity of GSH PX in blood and spinal cord tissue were similar to that in SOD No significant difference was observed between the blood transfusion and control groups Conclusion UBIO can ease free radical damages and elevate the activity of antioxidases after spinal cord injury in rabbits展开更多
[Objective] The aim of this paper is to investigate the influences of exogenous 5-aminolevulinic acid （5-aLA） application on the growth and mechanism of winter oilseed rape （Brassica napus L.） before winter. [Meth...[Objective] The aim of this paper is to investigate the influences of exogenous 5-aminolevulinic acid （5-aLA） application on the growth and mechanism of winter oilseed rape （Brassica napus L.） before winter. [Method] For this purpose a split-plot experimental design with four treatments of seed soaking with 0,25,50 and 75 mg/L ALA solution （CK,A1,A2 and A3） was carried out from October 2009 to January 2010 in order to observe the influences upon the growth and relevant physiological indexes of Zhongshuang 9. [Result] Seed soaking with ALA solution significantly promotes the growth of double-9 before winter,which is probably due to the fact that ALA application enhances its photosynthesis,absorption of nutrients,and its anti-oxidation abilities. The research also finds that ALA treatment notably increases its photosynthetic pigment contents,root activities,leaf nitrate reductase activities,soluble sugar contents,antioxidase activities,and significantly decreases malondialdehyde（MDA） content. [Conclusion] These results indicate that seed soaking with 50 mg/L ALA solution might have a potential application in increasing the productivity of winter oilseed rape in agricultural management.展开更多
[Objective] This study aimed to investigate the protective effects of aqueous extract of Eucommia ulmiodes Oliver leaves （AEO） on liver mitochondrial injury induced by free radicals. [Method] MDA content was determi...[Objective] This study aimed to investigate the protective effects of aqueous extract of Eucommia ulmiodes Oliver leaves （AEO） on liver mitochondrial injury induced by free radicals. [Method] MDA content was determined by thiobarbituric acid colorimetric method; mitochondrial swelling degree was determined by spectrophotometry; the superoxide anion radical scavenging ability was determined using reduced coenzyme I-tetrazolium-phenazine methosulfate as the superoxide anion generation system. Mice were hypodermically injected in the back and neck with D- galactose, after 50 d, the effects of AEO on the activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and anti-hydroxyl radicals in mouse liver were determined using kits. [Result] AEO can efficiently reduce the liver injury and inhibit mitochondrial swelling induced by Fe2＋-L-Cys, which can also scavenge superoxide anion and improve the activities of antioxidant enzymes. [Conclusion] This study provided scientific basis for the development and application of AEO resources.展开更多
BACKGROUND Fruits of Gardenia jasminoides(G.jasminoides)have extremely high medicinal value.However,the quality and traits of the plants vary significantly based on their provenances.In addition,the behaviour of the k...BACKGROUND Fruits of Gardenia jasminoides(G.jasminoides)have extremely high medicinal value.However,the quality and traits of the plants vary significantly based on their provenances.In addition,the behaviour of the known bioactive components,such as geniposide and crocin,has been the primary focus of the research on G.jasminoides.However,the identification of unknown bioactive components and their metabolomics remains underexplored.Therefore,analysing the metabolic differences between gardenias from different sources is essential to provide a comprehensive theoretical basis for the evaluation of G.jasminoides and germplasm resource identification.AIM To systematically evaluate the morphology,secondary metabolites,typical active ingredients,and antioxidant activity of wild G.jasminoides fruits.METHODS Gardenia fruits were collected from different provenances.Metabolites were identified via ultra-high-performance liquid chromatography–tandem mass spectrometry(UPLC-MS/MS).The metabolic differences were compared using hierarchical cluster analysis(HCA).The antioxidant capacity was evaluated using 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays and ferric reducing antioxidant power assays,and its correlation with typical active ingredients was analysed.RESULTS A total of 444 and 240 metabolites were identified using UPLC-MS/MS in positive and negative ion modes,respectively.The HCA results of the flavonoids indicated that the higher content of flavonoids was in the fruits from Lukou.The differential analysis of metabolites in fruits from Shaoyang,Miluo and Lukou showed that the fruit from Miluo had the highest upregulated differential metabolites.CONCLUSION The metabolic characteristics of the Ningxiang and Xiangxi extracts were similar,while those of Lukou,Miluo and Shaoyang extracts differed significantly.展开更多
文摘Objective The SOD, POD and APX enzyme activities of tetraploid lines and diploid control line of Chrysanthemum cinerariifolium in test-tube and field were determined in order to provide a reference for the selective breeding of high resistance good tetraploid varieties of C. cinerariifolium. Method The SOD, POD and APX activities of each sample were determined. Every material should have 3 replicates which been determined and calculated mean value to analyze. Result The various kinds of antioxidase activities in protective enzyme system of tetraploid lines were increased generally in comparison with that of diploid control line. And there is certain relativity between the samples in field and in test-tube of these lines. Conclusion The antioxidase activities of each line in test-tube could be considered as reference index to screen high resistance good tetraploid varieties of C. cinerariifolium.
基金Supported by Key Project of Provincial Department of Education for Scientific and Technological Research(12511061)~~
文摘[Objective] This study was conducted to determine the physiological and biochemical characteristics of potato tubers infected by Fusarium trichothecioides. [Method] The soluble protein content, SOD activity, POD activity and malondialdehyde （MDA） content of the tubers of two potato varieties Atlantic and Keshan 1 infected by F. trichothecioides were measured 0, 3, 6, 9, 12, 24 and 48 h later. [Result] Com- pared with the control uninfected, the contents of soluble protein and MDA in the tu- bers of both potato varieties infected with F. trichothecioides decreased, while the SOD activity increased, indicating that SOD activity had a certain relationship with the resistance of potato to dry rot. The POD activity in Keshan 1 which is resistant to F. trichothecioides was higher than in Atlantic, which is susceptible to F. trichothe- cioides, indicating that POD activity was closely related with potato＇s resistance to Fusarium dry rot. [Conclusion] The physiological and biochemical characteristics of potato tubers infected by F. trichothecioides will provide some references for the early waming and prevention of Fusariurn dry rot.
基金supported by the Public Service(Agriculture)Special Research Fund,China(nyhyzx07-2022)
文摘The regulatory mechanism of soil cadmium （Cd） on antioxidases in sorghum was studied using 3 sorghum species viz., sweet sorghum [Sorghum bicolor （L.）] Moench. cv. Hunnigreen, sorghum hybrid sudangrass （Sorghum bicolor × S. sudanense, cv. Everlush） and sudangrass [Sorghum sudanense （Piper） Stapf cv. Xinjiang]. The results showed that low concentrations of Cd^2＋ （410 mg kg-0 induced the activities of 4 antioxidases in leaves of the 3 species of sorghum, but high Cd^2＋ concentrations （≥ 50 mg kg-0 significantly reduced the activities of all the four antioxidases. The glutathione （GSH） contents in leaves of the 3 species of sorghum correlated positively with the Cd^2＋ concentrations. The maximum antioxidase activities were observed at the elongation stage of sorghum during the whole growth period.
基金Sponsored by Key Program of Sichuan Provincial Department of Education(12ZA149)
文摘With the potato variety Xishu 1 as the test materials, this paper adopted tissue culture to study the effect of Pb and Cd stress with different mass fractions on potato growth and the antioxidase system. The results showed that under the stress of Pb （200 mg/kg） and Cd （20 mg/kg）, growth of potato had no obvious difference with that in control group; under the stress of Pb （ 〉200 mg/kg） and Cd （ 〉20 mg/kg）, potato growth suspended, and its physiological indexes such as dry weight, plant height, root number, root length were inhibited obviously. With the increasing mass fraction of Pb, relative electrical conductivity, MDA and proline of potato leaves showed the tendency of increase, and the results in high mass fraction treatments showed significant difference with those in the control group （P 〈 0.05 ）, CAT, POD and SOD activity of leaves increased greatly with the increasing mass fraction. In the Pb stress treatment （200 mg/kg）, relative electrical conductivity, MDA and proline of potato leaves showed no obvious difference with those in the control group, and the results in high mass fraction treatments were significantly higher than those in the control group （ P 〈 O. 05 ）, CAT and SOD activity increased greatly with the increasing mass fraction （ P 〈 0.05 ）. Under the stress of Pb and Cd, synthesis of soluble protein in potato leaves was strongly inhibited. The results showed that potato was tolerant to Pb and Cd of lower mass fraction, and damage of potato caused by Pb and Cd stress could be relieved by improving activity of antioxidase and reducing peroxidation of membrane lipid, and the tolerance reduced with the increase of stress.
文摘To investigate the effects of ultraviolet blood irradiation and oxygenation (UBIO) on free radicals and antioxidase after spinal cord injury in rabbits Methods Totally, 186 rabbits were used and divided randomly into four experimental groups: control (n=6), blood transfusion (n=24), injured (n=96) and treatment (n=60) groups The relative intensity of free radical (FR) signals, malondialdehyde (MDA) content, as well as the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH PX) were compared among the four groups at 6, 24, 48, and 72 hours and 6 days after injury Results The relative intensity of FR signals in spinal cord tissue in the injured group increased at 48 hours and showed a striking difference compared with the control group; in the treatment group, it decreased and showed a striking difference compared with the injured group MDA content in blood in the injured group increased and showed a striking difference at 6, 24 and 48 hours and showed a significant difference at 72 hours and 6 days after injury compared with the control group In the treatment group, MDA content in blood decreased and showed a significant difference at 48 hours compared with the injured group MDA content in spinal cord tissue increased in the injured group and showed a striking difference compared with the control group; in the treatment group, it decreased and showed a striking difference compared with the injured group at the corresponding times The activity of SOD in blood and spinal cord tissue decreased in the injured group and showed a striking difference compared with the control group; in the treatment group, it increased and showed a striking difference compared with the injured group at the corresponding times The changes in activity of GSH PX in blood and spinal cord tissue were similar to that in SOD No significant difference was observed between the blood transfusion and control groups Conclusion UBIO can ease free radical damages and elevate the activity of antioxidases after spinal cord injury in rabbits
基金Supported by the Special Funds for Public Welfare Industry (Agriculture) Study of China (200903003 )the Subjects of Special Funds of Public Welfare Institutes of China (1610172011016)~~
文摘[Objective] The aim of this paper is to investigate the influences of exogenous 5-aminolevulinic acid （5-aLA） application on the growth and mechanism of winter oilseed rape （Brassica napus L.） before winter. [Method] For this purpose a split-plot experimental design with four treatments of seed soaking with 0,25,50 and 75 mg/L ALA solution （CK,A1,A2 and A3） was carried out from October 2009 to January 2010 in order to observe the influences upon the growth and relevant physiological indexes of Zhongshuang 9. [Result] Seed soaking with ALA solution significantly promotes the growth of double-9 before winter,which is probably due to the fact that ALA application enhances its photosynthesis,absorption of nutrients,and its anti-oxidation abilities. The research also finds that ALA treatment notably increases its photosynthetic pigment contents,root activities,leaf nitrate reductase activities,soluble sugar contents,antioxidase activities,and significantly decreases malondialdehyde（MDA） content. [Conclusion] These results indicate that seed soaking with 50 mg/L ALA solution might have a potential application in increasing the productivity of winter oilseed rape in agricultural management.
基金Supported by Fund for Scientific Research of Shaanxi Xueqian Normal University (2013KJ033)~~
文摘[Objective] This study aimed to investigate the protective effects of aqueous extract of Eucommia ulmiodes Oliver leaves （AEO） on liver mitochondrial injury induced by free radicals. [Method] MDA content was determined by thiobarbituric acid colorimetric method; mitochondrial swelling degree was determined by spectrophotometry; the superoxide anion radical scavenging ability was determined using reduced coenzyme I-tetrazolium-phenazine methosulfate as the superoxide anion generation system. Mice were hypodermically injected in the back and neck with D- galactose, after 50 d, the effects of AEO on the activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and anti-hydroxyl radicals in mouse liver were determined using kits. [Result] AEO can efficiently reduce the liver injury and inhibit mitochondrial swelling induced by Fe2＋-L-Cys, which can also scavenge superoxide anion and improve the activities of antioxidant enzymes. [Conclusion] This study provided scientific basis for the development and application of AEO resources.
基金Supported by The Forestry Science and Technology Innovation Funded Project of Hunan Forestry Bureau,No.XLKY202221。
文摘BACKGROUND Fruits of Gardenia jasminoides(G.jasminoides)have extremely high medicinal value.However,the quality and traits of the plants vary significantly based on their provenances.In addition,the behaviour of the known bioactive components,such as geniposide and crocin,has been the primary focus of the research on G.jasminoides.However,the identification of unknown bioactive components and their metabolomics remains underexplored.Therefore,analysing the metabolic differences between gardenias from different sources is essential to provide a comprehensive theoretical basis for the evaluation of G.jasminoides and germplasm resource identification.AIM To systematically evaluate the morphology,secondary metabolites,typical active ingredients,and antioxidant activity of wild G.jasminoides fruits.METHODS Gardenia fruits were collected from different provenances.Metabolites were identified via ultra-high-performance liquid chromatography–tandem mass spectrometry(UPLC-MS/MS).The metabolic differences were compared using hierarchical cluster analysis(HCA).The antioxidant capacity was evaluated using 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays and ferric reducing antioxidant power assays,and its correlation with typical active ingredients was analysed.RESULTS A total of 444 and 240 metabolites were identified using UPLC-MS/MS in positive and negative ion modes,respectively.The HCA results of the flavonoids indicated that the higher content of flavonoids was in the fruits from Lukou.The differential analysis of metabolites in fruits from Shaoyang,Miluo and Lukou showed that the fruit from Miluo had the highest upregulated differential metabolites.CONCLUSION The metabolic characteristics of the Ningxiang and Xiangxi extracts were similar,while those of Lukou,Miluo and Shaoyang extracts differed significantly.
