Urinary tract infections (UTIs) caused by uropathogens are a significant public health problem, and their treatment primarily relies on antibiotic therapy. However, the increasing global development of antibiotic resi...Urinary tract infections (UTIs) caused by uropathogens are a significant public health problem, and their treatment primarily relies on antibiotic therapy. However, the increasing global development of antibiotic resistance necessitates updating diagnostic techniques to ensure higher sensitivity and specificity, especially with advancements in science and medicine. This study aimed to evaluate the prevalence of UTIs and antibiotic resistance profiles through urine culture, as well as to identify Klebsiella pneumoniae, Klebsiella oxytoca, and Acinetobacter spp. in urine samples using a molecular approach with multiplex real-time PCR. From May 3 to July 25, 2023, at the Pietro Annigoni Biomolecular Research Center (CERBA) and Saint Camille Hospital of Ouagadougou (HOSCO), 209 urine samples collected from patients with suspected UTIs were analyzed using both urine culture and multiplex real-time PCR. Among the 209 patients, 52.15% were male and 47.85% female, with an average age of 46.87 ± 21.33 years. Urine cultures revealed an overall UTI prevalence of 23.44%, with a prevalence of 8.13% in men versus 15.31% in women (P = 0.023). The bacterial prevalence rates were as follows: Escherichia coli (12.92%), Klebsiella spp. (7.18%), Enterobacter cloacae (1.44%), Staphylococcus aureus (0.96%), and other bacteria. Klebsiella spp. demonstrated 100% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, while Escherichia coli showed 96.2% and 65.4% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, respectively. PCR analysis of the target bacteria revealed mono-infection prevalence rates of Klebsiella pneumoniae (10.39%), Klebsiella oxytoca (7.79%), and Acinetobacter spp. (7.79%), along with a co-infection prevalence rate of Klebsiella pneumoniae/Acinetobacter spp. (1.30%). This study demonstrated that PCR, with its high sensitivity and specificity, could effectively distinguish Klebsiella pneumoniae from Klebsiella oxytoca and detect Acinetobacter spp. in less than 24 hours—something urine culture alone could not achieve. The relative ease of automating urine PCR testing, combined with its diagnostic accuracy and rapid turnaround time, makes it a valuable addition to modern medical practice for the laboratory diagnosis of UTIs.展开更多
Achillea species are known for their healing properties since ancient times.There is extensive literature on their pharmacological action due to their bioactive compounds.The present study aimed to investigate the ant...Achillea species are known for their healing properties since ancient times.There is extensive literature on their pharmacological action due to their bioactive compounds.The present study aimed to investigate the antioxidant and antimicrobial effects of hydroalcoholic extracts from the inflorescences and leaves of the species Achillea crithmifolia Waldst.and Kit.,A.grandifolia Friv.and A.millefolium L.The phytochemical profiles of all extracts were evaluated both by NMR spectroscopy and LC-MS analysis,and the results were consistent with the spectrophotometrically determined total phenolic(TP:125.42-191.98 mg/g)and total flavonoid(TF:47.34-180.02 mg/g)contents.All the extracts were tested for their antioxidant activity using DPPH and ABTS•+radical scavenging assay,as well as ferrous ion chelating ability and reducing power tests.All the extracts showed moderate antioxidant activity,compared to the reference substance BHT.Additionally,the antibacterial activity of the extracts was evaluated against major food-borne pathogens,showing moderate antimicrobial effects.展开更多
Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short...Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short-chain fatty acids,and influence immune responses.However,their diversity and functional differences have created challenges for their development and therapeutic use.Recent studies have shown that specific Prevotella species,such as P.copri,P.intestinalis,and P.histicola,can strengthen gut barrier integrity and reduce metabolic imbalances.Notably,Prevotella populations can be increased through high-fiber or herbal-based treatments.Traditional herbal medicines,including fiber-rich decoctions,also demonstrate the potential to boost endogenous Prevotella communities,enhance microbial fermentation,and improve glucose and lipid balance.This perspective examines the context-dependent roles of Prevotella spp.,with emphasis on the functional heterogeneity of key species such as P.copri,suggests a framework for combining herbal modulation with species-level microbiota profiling,and outlines a research plan to explore microbe-herb synergy in treating obesity,type 2 diabetes,and related metabolic disorders.This strategy offers a new,ecology-based approach to complement standard metabolic interventions.展开更多
Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100...Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100 COPD subjects from our hospital from January 2020 to December 2023 were included and randomly divided into a healthy control group and an experimental group(50 cases each).The healthy control group underwent slow vital capacity measurement using a spirometer,while the experimental group underwent high-resolution thin-slice CT scans and serum Vanin-1 and SPP1 concentration measurements.Pulmonary function parameters,symptom burden,biomarker concentrations,and imaging characteristics were compared between the two groups.Results:The FEV1/FVC ratio in the experimental group(58.3±7.2)was lower than that in the healthy control group(92.1±4.8);the total CAT score(22.4±3.5)was higher than that in the healthy control group(3.1±1.2);both Vanin-1(18.7±2.3μg/L)and SPP1(25.6±4.1μg/L)levels were higher than those in the healthy control group;LAA%-950(38.7±6.2%)and WA%(68.5±5.3%)were significantly higher than those in the healthy control group(all p<0.001).Conclusion:Chest CT combined with serum Vanin-1 and SPP1 can accurately quantify the pathological progression of COPD,providing a dual basis for clinical staging and individualized intervention.展开更多
A total of 10 non-repetitive multi-drug-resistant Acinetobacter strains were collected.With reference to A.calcoaceticus(ATCC23055),A.baumannii(ATCC19606),A.lwoffii(ATCC17986),and A.junii(NCTC5866),DNA fingerprint tec...A total of 10 non-repetitive multi-drug-resistant Acinetobacter strains were collected.With reference to A.calcoaceticus(ATCC23055),A.baumannii(ATCC19606),A.lwoffii(ATCC17986),and A.junii(NCTC5866),DNA fingerprint technique,amplified ribosomal DNA restriction analysis(ARDRA),and random amplified polymorphism DNA(RAPD)were carried out to identify the genomic species of Acinetobacter spp.The distances between them were calculated by the unweighted pair group method with arithmetic(UPGMA).Genotypes of Acinetobacter spp.were effectively classified and an A.junii together with nine A.baumannii isolates was genomically identified.The combination of ARDRA and RAPD DNA-fingerprint technique shows high complementarity,and could be a useful tool in Acinetobacter genomic species identification.展开更多
Objective:To investigate the pattern of antibiotic resistance and biofilm production capabilities of clinical Acinetobacter baumannii(A.baumannii)isolates in this study.Methods:A.baumannii isolates were collected from...Objective:To investigate the pattern of antibiotic resistance and biofilm production capabilities of clinical Acinetobacter baumannii(A.baumannii)isolates in this study.Methods:A.baumannii isolates were collected from Tehran Imam Khomeini Hospital in this cross-sectional study,and the minimum inhibitory concentrations for 16 antibiotics were determined using Vitek2®systems.All isolates were analyzed for biofilm production,then presence of biofilm-associated genes,and class Ⅰ and Ⅱ integron genes.Results:60 non-replicate A.baumannii isolates were included in this study.The resistance rates reached 100%for aztreonam,cefepime,ceftazidime,ciprofloxacin,piperacillin-tazobactam,piperacillin,ticarcillin,and trimethoprim-sulfamethoxazole.A.baumannii isolates were most sensitive to colistin and rifampicin being the most effective treatments.Multi-drug resistant and extensively drug-resistant isolates accounted for 83.3%and 16.7%,respectively.Of the isolates,91.6%formed biofilms,categorized as 10%strong,31.6%moderate,and 50%weak.No correlation was found between antibiotic resistance and biofilm formation.The genes csuE,abaI,and ompA were prevalent,but their distribution was similar across biofilm categories.A relationship between Int1 and biofilm production was noted.Conclusions:The high rates of antibiotic resistance and biofilm formation,alongside the presence of integrons including class Ⅰ and Ⅱ,underscore the necessity for ongoing monitoring of A.baumannii.Notably,classⅠintegron presence was significantly linked to biofilm formation.Further research is needed to explore the connection between antibiotic resistance and biofilm production in A.baumannii.展开更多
Microorganisms inhabiting soils contaminated with heavy metals produce melanin,a dark brown pigment,as a survival strategy.In this study,a melanin-producing bacterium,Acinetobacter sp.ME1,with heavy metal tolerance an...Microorganisms inhabiting soils contaminated with heavy metals produce melanin,a dark brown pigment,as a survival strategy.In this study,a melanin-producing bacterium,Acinetobacter sp.ME1,with heavy metal tolerance and plant growth-promoting traits,was isolated from abandoned mine soil.Strain ME1 exhibited growth at concentrations of Zn up to 250 mg/L,Cd and Pb up to 100 mg/L,and Cr up to 50 mg/L.It had the ability to produce the plant hormone indole-3-acetic acid and siderophores along with 1-aminocyclopropane-1-carboxylic acid deaminase and protease activities.Additionally,it showed antioxidant activity,including catalase and 2,2-diphenyl-1-picryhydrazyl(DPPH)scavenging activities.The optimal conditions for melanin production by ME1 were a pH of 7 and a temperature of 35℃.At 1000 mg/L,ME1-extracted melanin exhibited DPPH radical scavenging activity of(25.040±0.007)%,a sun protection factor of 15.200±0.260,and 19.6%antibacterial activity against the plant pathogen Xanthomonas campestris.Furthermore,its adsorption capacity was(0.235±0.073)mg/g melanin for Zn and(0.277±0.008)mg/g melanin for Ni.In plants of Brassica chinensis grown under conditions of hydroponic cultivation with single heavy metal contamination of Cd,Zn,Pb,or Cr,the removal efficiency of each heavy metal was improved by 0.1–1.8 times after 3 d following inoculation with the strain ME1 compared to the plants grown under the same conditions without inoculation.In addition,ME1 inoculation improved the removal efficiency of each heavy metal by 0.1–1.0 times under multiple heavy metal contamination conditions.These findings suggest that Acinetobacter sp.ME1 could be used to enhance phytoremediation efficiency in heavy metal-contaminated soils.Moreover,the melanin it produces also holds promise in cosmetics,household products,and medical applications due to its photoprotective,antioxidant,and antimicrobial properties.展开更多
The mortality rate ofAcinetobacter baumannii is as high as 47%,This is inseparable From its strong resistance to carbapenems,polymyxins,aminoglycosides,quinolone antibiotics.This article will analyze the resistance of...The mortality rate ofAcinetobacter baumannii is as high as 47%,This is inseparable From its strong resistance to carbapenems,polymyxins,aminoglycosides,quinolone antibiotics.This article will analyze the resistance of Acinetobacter baumannii to carbapenem antibiotics(meropenem and imipenem)and explore why Acinetobacter baumannii is so resistant.The data I used comes from the NCBI database,which includes six resistant groups(R)to meropenem and imipenem,and five sensitive groups(S).The level of the resistant group is complete genome,three sensitive groups are contig,and two are complete genome.Subsequently,I used Prokka in Galaxy to convert the Fasta.file into various forms such as Faa,gbk,ffn.After performing Fisher's test on all GFF files,I obtained the result chart,which includes the search for relevant domains and protein descriptions on Interpro using the chart.ProteinPlus also performed drug pocket prediction and constructed protein models using Swissmodel,as well as different multi sequence comparisons.Finally,I analyzed the functions of the proteins corresponding to the genes specific to the resistance group and the reasons for the development of drug resistance,making future research on the drug resistance of Acinetobacter baumannii easier.And further research can be conducted on the drug pocket in the protein ofAcinetobacter baumannii that has developed resistance,and drugs can be administered.展开更多
Background:Pseudomonas aeruginosa and Acinetobacter baumannii are common nosocomial pathogens in hospital settings.Recently,they have also been found in non-hospital environments,such as poultry farms.While most studi...Background:Pseudomonas aeruginosa and Acinetobacter baumannii are common nosocomial pathogens in hospital settings.Recently,they have also been found in non-hospital environments,such as poultry farms.While most studies in Ethiopia have focused on these bacteria's antibiotic resistance patterns in hospitals,information regarding their prevalence and resistance in veterinary settings,particularly poultry farms,is limited.This study aimed to assess the prevalence and antibiotic resistance patterns of P.aeruginosa and A.baumannii isolated from chicken droppings on poultry farms.Methods:A cross-sectional study was conducted from March 2022 to June 2022.A total of 87 poultry farms were included in this study,and pooled chicken dropping samples were collected.The samples were subsequently transferred to buffered peptone water and cultured on MacConkey agar.Species of the isolates were identified via routine biochemical tests,including oxidase,catalase,urease,Simon's citrate,sulfide indole motility medium,triple sugar iron agar and growth at temperatures of 37℃ and 42℃.The Kirby–Bauer disk diffusion technique was used for antibiotic susceptibility testing.The data were entered into EpiData version 4.6 and then exported to SPSS version 26 for analysis.Fisher's exact test was used to observe an appropriate association between independent variables and the occurrence of isolates.The results are presented in the text,figures and tables.Results:Among the 87 poultry farms,41(47.1%)were positive for Pseudomonas and Acinetobacter.Among these strains,24(27.6%)P.aeruginosa strains and 13(14.9%)A.baumannii strains were recovered.P.aeruginosa showed complete resistance to tetracycline(24,100.0%)and trimethoprim-sulfamethoxazole(24,100.0%).Additionally,there was a high rate of resistance to ciprofloxacin(13,54.2%)and amikacin(12,50.0%).Similarly,13(100.0%)A.baumannii isolates were resistant to tetracycline,and 12(92.3%)were resistant to trimethoprim-sulfamethoxazole.However,both isolates presented lower resistance rates to piperacillintazobactam(4,9.8%)and cefepime(7,17.1%).Both A.baumannii and P.aeruginosa exhibited multidrug resistance in 10/13(76.9%)and 16/24(66.7%)of the strains,respectively.The overall prevalence of multidrug resistance in this study was 28/41(68.3%).Conclusion:This study demonstrated that poultry farms may be potential reservoirs for P.aeruginosa and A.baumannii,including antibiotic-resistant strains.This is a significant concern to public health because poultry farmers may be contaminated,increasing their dissemination to the community.Therefore,poultry farmers should improve sanitation and reduce the misuse and overuse of antibiotics at poultry farms.展开更多
Fournier’s gangrene is a rare urological condition with a poor prognosis and an extremely high mortality rate.Infections caused by pathogenic microorganisms play a critical role in the pathogenesis of Fournier’s gan...Fournier’s gangrene is a rare urological condition with a poor prognosis and an extremely high mortality rate.Infections caused by pathogenic microorganisms play a critical role in the pathogenesis of Fournier’s gangrene.Rapid assessment and thorough debridement are crucial for survival and prognosis of patients with this disease.The present case involved a 62-year-old male patient with poorly controlled diabetes,who presented with unexplained scrotal swelling for 2 days at a local hospital where scrotal surgical debridement was performed.However,the procedure was unsuccessful.This case was characterized by rapid disease progression,widespread wound involvement,and dual infection with multidrug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa.Our team performed early,extensive surgical debridement and,based on the results of antimicrobial susceptibility testing,initiated combination antibiotic therapy.The patient’s condition improved significantly after these interventions.However,the treatment was ultimately discontinued by the patient’s family for personal reasons,and follow-up care was declined.展开更多
Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter...Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter spp.in PIF incidents occurs from time to time,causing infant serious diseases or death.In this investigation,matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify the phenotypes of 35 Cronobacter strains isolated from PIF and its processing environment.Subsequently,the isolates were evaluated for drying and osmotic pressure tolerance.The results showed that the deactivation rate of the strains ranged from 9.01%to 77.57%,and the highest osmotic pressure condition the strains could tolerate was 6 g/100 mL Na Cl.In addition,there was a positive correlation between biofilm formation ability and desiccation resistance.Combined with transcriptomics,Cronobacter spp.could activate biofilm synthesis,produce more trehalose,accumulate betaine and electrolytes to stabilize intracellular structure under the two treatment conditions.A total of 31 and 43 genes were found related to desiccation and permeability resistance,respectively.And some genes(cysM,thuF,ycjO,etc.)were found to be associated with two tolerances for the first time.展开更多
文摘Urinary tract infections (UTIs) caused by uropathogens are a significant public health problem, and their treatment primarily relies on antibiotic therapy. However, the increasing global development of antibiotic resistance necessitates updating diagnostic techniques to ensure higher sensitivity and specificity, especially with advancements in science and medicine. This study aimed to evaluate the prevalence of UTIs and antibiotic resistance profiles through urine culture, as well as to identify Klebsiella pneumoniae, Klebsiella oxytoca, and Acinetobacter spp. in urine samples using a molecular approach with multiplex real-time PCR. From May 3 to July 25, 2023, at the Pietro Annigoni Biomolecular Research Center (CERBA) and Saint Camille Hospital of Ouagadougou (HOSCO), 209 urine samples collected from patients with suspected UTIs were analyzed using both urine culture and multiplex real-time PCR. Among the 209 patients, 52.15% were male and 47.85% female, with an average age of 46.87 ± 21.33 years. Urine cultures revealed an overall UTI prevalence of 23.44%, with a prevalence of 8.13% in men versus 15.31% in women (P = 0.023). The bacterial prevalence rates were as follows: Escherichia coli (12.92%), Klebsiella spp. (7.18%), Enterobacter cloacae (1.44%), Staphylococcus aureus (0.96%), and other bacteria. Klebsiella spp. demonstrated 100% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, while Escherichia coli showed 96.2% and 65.4% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, respectively. PCR analysis of the target bacteria revealed mono-infection prevalence rates of Klebsiella pneumoniae (10.39%), Klebsiella oxytoca (7.79%), and Acinetobacter spp. (7.79%), along with a co-infection prevalence rate of Klebsiella pneumoniae/Acinetobacter spp. (1.30%). This study demonstrated that PCR, with its high sensitivity and specificity, could effectively distinguish Klebsiella pneumoniae from Klebsiella oxytoca and detect Acinetobacter spp. in less than 24 hours—something urine culture alone could not achieve. The relative ease of automating urine PCR testing, combined with its diagnostic accuracy and rapid turnaround time, makes it a valuable addition to modern medical practice for the laboratory diagnosis of UTIs.
基金supported by the Serbian Ministry of Education,Science and Technological Development(Agreements No.451-03-136/2025-03/200122 and 451-03-136/2025-03/200378).
文摘Achillea species are known for their healing properties since ancient times.There is extensive literature on their pharmacological action due to their bioactive compounds.The present study aimed to investigate the antioxidant and antimicrobial effects of hydroalcoholic extracts from the inflorescences and leaves of the species Achillea crithmifolia Waldst.and Kit.,A.grandifolia Friv.and A.millefolium L.The phytochemical profiles of all extracts were evaluated both by NMR spectroscopy and LC-MS analysis,and the results were consistent with the spectrophotometrically determined total phenolic(TP:125.42-191.98 mg/g)and total flavonoid(TF:47.34-180.02 mg/g)contents.All the extracts were tested for their antioxidant activity using DPPH and ABTS•+radical scavenging assay,as well as ferrous ion chelating ability and reducing power tests.All the extracts showed moderate antioxidant activity,compared to the reference substance BHT.Additionally,the antibacterial activity of the extracts was evaluated against major food-borne pathogens,showing moderate antimicrobial effects.
基金supported by the National Research Foundation of Korea(2020R1F1A1074155).
文摘Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short-chain fatty acids,and influence immune responses.However,their diversity and functional differences have created challenges for their development and therapeutic use.Recent studies have shown that specific Prevotella species,such as P.copri,P.intestinalis,and P.histicola,can strengthen gut barrier integrity and reduce metabolic imbalances.Notably,Prevotella populations can be increased through high-fiber or herbal-based treatments.Traditional herbal medicines,including fiber-rich decoctions,also demonstrate the potential to boost endogenous Prevotella communities,enhance microbial fermentation,and improve glucose and lipid balance.This perspective examines the context-dependent roles of Prevotella spp.,with emphasis on the functional heterogeneity of key species such as P.copri,suggests a framework for combining herbal modulation with species-level microbiota profiling,and outlines a research plan to explore microbe-herb synergy in treating obesity,type 2 diabetes,and related metabolic disorders.This strategy offers a new,ecology-based approach to complement standard metabolic interventions.
基金Baoding Science and Technology Plan Project(Project No.:2341ZF214)。
文摘Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100 COPD subjects from our hospital from January 2020 to December 2023 were included and randomly divided into a healthy control group and an experimental group(50 cases each).The healthy control group underwent slow vital capacity measurement using a spirometer,while the experimental group underwent high-resolution thin-slice CT scans and serum Vanin-1 and SPP1 concentration measurements.Pulmonary function parameters,symptom burden,biomarker concentrations,and imaging characteristics were compared between the two groups.Results:The FEV1/FVC ratio in the experimental group(58.3±7.2)was lower than that in the healthy control group(92.1±4.8);the total CAT score(22.4±3.5)was higher than that in the healthy control group(3.1±1.2);both Vanin-1(18.7±2.3μg/L)and SPP1(25.6±4.1μg/L)levels were higher than those in the healthy control group;LAA%-950(38.7±6.2%)and WA%(68.5±5.3%)were significantly higher than those in the healthy control group(all p<0.001).Conclusion:Chest CT combined with serum Vanin-1 and SPP1 can accurately quantify the pathological progression of COPD,providing a dual basis for clinical staging and individualized intervention.
基金This study was supported by the Natural Science Research Program of Universities and Colleges of Anhui Province,China(No.2006kj349B).
文摘A total of 10 non-repetitive multi-drug-resistant Acinetobacter strains were collected.With reference to A.calcoaceticus(ATCC23055),A.baumannii(ATCC19606),A.lwoffii(ATCC17986),and A.junii(NCTC5866),DNA fingerprint technique,amplified ribosomal DNA restriction analysis(ARDRA),and random amplified polymorphism DNA(RAPD)were carried out to identify the genomic species of Acinetobacter spp.The distances between them were calculated by the unweighted pair group method with arithmetic(UPGMA).Genotypes of Acinetobacter spp.were effectively classified and an A.junii together with nine A.baumannii isolates was genomically identified.The combination of ARDRA and RAPD DNA-fingerprint technique shows high complementarity,and could be a useful tool in Acinetobacter genomic species identification.
基金This study was financially supported by the Vice Chancellor for Research Affairs,Ilam University of Medical Sciences,Ilam,Iran(Project No.1326).
文摘Objective:To investigate the pattern of antibiotic resistance and biofilm production capabilities of clinical Acinetobacter baumannii(A.baumannii)isolates in this study.Methods:A.baumannii isolates were collected from Tehran Imam Khomeini Hospital in this cross-sectional study,and the minimum inhibitory concentrations for 16 antibiotics were determined using Vitek2®systems.All isolates were analyzed for biofilm production,then presence of biofilm-associated genes,and class Ⅰ and Ⅱ integron genes.Results:60 non-replicate A.baumannii isolates were included in this study.The resistance rates reached 100%for aztreonam,cefepime,ceftazidime,ciprofloxacin,piperacillin-tazobactam,piperacillin,ticarcillin,and trimethoprim-sulfamethoxazole.A.baumannii isolates were most sensitive to colistin and rifampicin being the most effective treatments.Multi-drug resistant and extensively drug-resistant isolates accounted for 83.3%and 16.7%,respectively.Of the isolates,91.6%formed biofilms,categorized as 10%strong,31.6%moderate,and 50%weak.No correlation was found between antibiotic resistance and biofilm formation.The genes csuE,abaI,and ompA were prevalent,but their distribution was similar across biofilm categories.A relationship between Int1 and biofilm production was noted.Conclusions:The high rates of antibiotic resistance and biofilm formation,alongside the presence of integrons including class Ⅰ and Ⅱ,underscore the necessity for ongoing monitoring of A.baumannii.Notably,classⅠintegron presence was significantly linked to biofilm formation.Further research is needed to explore the connection between antibiotic resistance and biofilm production in A.baumannii.
基金supported by the National Research Foundation of Korea(NRF)grants funded by the Korean government,the Ministry of Science,and the Ministry of Science and Information and Communication Technology(ICT(MSIT))(Nos.2022R1A2C2006615 and RS2023-00217228).
文摘Microorganisms inhabiting soils contaminated with heavy metals produce melanin,a dark brown pigment,as a survival strategy.In this study,a melanin-producing bacterium,Acinetobacter sp.ME1,with heavy metal tolerance and plant growth-promoting traits,was isolated from abandoned mine soil.Strain ME1 exhibited growth at concentrations of Zn up to 250 mg/L,Cd and Pb up to 100 mg/L,and Cr up to 50 mg/L.It had the ability to produce the plant hormone indole-3-acetic acid and siderophores along with 1-aminocyclopropane-1-carboxylic acid deaminase and protease activities.Additionally,it showed antioxidant activity,including catalase and 2,2-diphenyl-1-picryhydrazyl(DPPH)scavenging activities.The optimal conditions for melanin production by ME1 were a pH of 7 and a temperature of 35℃.At 1000 mg/L,ME1-extracted melanin exhibited DPPH radical scavenging activity of(25.040±0.007)%,a sun protection factor of 15.200±0.260,and 19.6%antibacterial activity against the plant pathogen Xanthomonas campestris.Furthermore,its adsorption capacity was(0.235±0.073)mg/g melanin for Zn and(0.277±0.008)mg/g melanin for Ni.In plants of Brassica chinensis grown under conditions of hydroponic cultivation with single heavy metal contamination of Cd,Zn,Pb,or Cr,the removal efficiency of each heavy metal was improved by 0.1–1.8 times after 3 d following inoculation with the strain ME1 compared to the plants grown under the same conditions without inoculation.In addition,ME1 inoculation improved the removal efficiency of each heavy metal by 0.1–1.0 times under multiple heavy metal contamination conditions.These findings suggest that Acinetobacter sp.ME1 could be used to enhance phytoremediation efficiency in heavy metal-contaminated soils.Moreover,the melanin it produces also holds promise in cosmetics,household products,and medical applications due to its photoprotective,antioxidant,and antimicrobial properties.
文摘The mortality rate ofAcinetobacter baumannii is as high as 47%,This is inseparable From its strong resistance to carbapenems,polymyxins,aminoglycosides,quinolone antibiotics.This article will analyze the resistance of Acinetobacter baumannii to carbapenem antibiotics(meropenem and imipenem)and explore why Acinetobacter baumannii is so resistant.The data I used comes from the NCBI database,which includes six resistant groups(R)to meropenem and imipenem,and five sensitive groups(S).The level of the resistant group is complete genome,three sensitive groups are contig,and two are complete genome.Subsequently,I used Prokka in Galaxy to convert the Fasta.file into various forms such as Faa,gbk,ffn.After performing Fisher's test on all GFF files,I obtained the result chart,which includes the search for relevant domains and protein descriptions on Interpro using the chart.ProteinPlus also performed drug pocket prediction and constructed protein models using Swissmodel,as well as different multi sequence comparisons.Finally,I analyzed the functions of the proteins corresponding to the genes specific to the resistance group and the reasons for the development of drug resistance,making future research on the drug resistance of Acinetobacter baumannii easier.And further research can be conducted on the drug pocket in the protein ofAcinetobacter baumannii that has developed resistance,and drugs can be administered.
文摘Background:Pseudomonas aeruginosa and Acinetobacter baumannii are common nosocomial pathogens in hospital settings.Recently,they have also been found in non-hospital environments,such as poultry farms.While most studies in Ethiopia have focused on these bacteria's antibiotic resistance patterns in hospitals,information regarding their prevalence and resistance in veterinary settings,particularly poultry farms,is limited.This study aimed to assess the prevalence and antibiotic resistance patterns of P.aeruginosa and A.baumannii isolated from chicken droppings on poultry farms.Methods:A cross-sectional study was conducted from March 2022 to June 2022.A total of 87 poultry farms were included in this study,and pooled chicken dropping samples were collected.The samples were subsequently transferred to buffered peptone water and cultured on MacConkey agar.Species of the isolates were identified via routine biochemical tests,including oxidase,catalase,urease,Simon's citrate,sulfide indole motility medium,triple sugar iron agar and growth at temperatures of 37℃ and 42℃.The Kirby–Bauer disk diffusion technique was used for antibiotic susceptibility testing.The data were entered into EpiData version 4.6 and then exported to SPSS version 26 for analysis.Fisher's exact test was used to observe an appropriate association between independent variables and the occurrence of isolates.The results are presented in the text,figures and tables.Results:Among the 87 poultry farms,41(47.1%)were positive for Pseudomonas and Acinetobacter.Among these strains,24(27.6%)P.aeruginosa strains and 13(14.9%)A.baumannii strains were recovered.P.aeruginosa showed complete resistance to tetracycline(24,100.0%)and trimethoprim-sulfamethoxazole(24,100.0%).Additionally,there was a high rate of resistance to ciprofloxacin(13,54.2%)and amikacin(12,50.0%).Similarly,13(100.0%)A.baumannii isolates were resistant to tetracycline,and 12(92.3%)were resistant to trimethoprim-sulfamethoxazole.However,both isolates presented lower resistance rates to piperacillintazobactam(4,9.8%)and cefepime(7,17.1%).Both A.baumannii and P.aeruginosa exhibited multidrug resistance in 10/13(76.9%)and 16/24(66.7%)of the strains,respectively.The overall prevalence of multidrug resistance in this study was 28/41(68.3%).Conclusion:This study demonstrated that poultry farms may be potential reservoirs for P.aeruginosa and A.baumannii,including antibiotic-resistant strains.This is a significant concern to public health because poultry farmers may be contaminated,increasing their dissemination to the community.Therefore,poultry farmers should improve sanitation and reduce the misuse and overuse of antibiotics at poultry farms.
基金supported by the Collaborative Innovation Center of China’s Ministry of Education(grant no.2020-39)Constructive Project of Innovative Talent Platform for Precise Repair of Wounds(grant no.2021-3)+1 种基金Scientific Research and Talent Training Fund of Kweichow Moutai Hospital(grant no.2022-13)Shanghai Wang Zhengguo Trauma Medicine Development Foundation(grant no.SZYZ-TR-05).
文摘Fournier’s gangrene is a rare urological condition with a poor prognosis and an extremely high mortality rate.Infections caused by pathogenic microorganisms play a critical role in the pathogenesis of Fournier’s gangrene.Rapid assessment and thorough debridement are crucial for survival and prognosis of patients with this disease.The present case involved a 62-year-old male patient with poorly controlled diabetes,who presented with unexplained scrotal swelling for 2 days at a local hospital where scrotal surgical debridement was performed.However,the procedure was unsuccessful.This case was characterized by rapid disease progression,widespread wound involvement,and dual infection with multidrug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa.Our team performed early,extensive surgical debridement and,based on the results of antimicrobial susceptibility testing,initiated combination antibiotic therapy.The patient’s condition improved significantly after these interventions.However,the treatment was ultimately discontinued by the patient’s family for personal reasons,and follow-up care was declined.
基金supported by the Joint Funds of the National Natural Science Foundation of China(U21A20272)。
文摘Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter spp.in PIF incidents occurs from time to time,causing infant serious diseases or death.In this investigation,matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify the phenotypes of 35 Cronobacter strains isolated from PIF and its processing environment.Subsequently,the isolates were evaluated for drying and osmotic pressure tolerance.The results showed that the deactivation rate of the strains ranged from 9.01%to 77.57%,and the highest osmotic pressure condition the strains could tolerate was 6 g/100 mL Na Cl.In addition,there was a positive correlation between biofilm formation ability and desiccation resistance.Combined with transcriptomics,Cronobacter spp.could activate biofilm synthesis,produce more trehalose,accumulate betaine and electrolytes to stabilize intracellular structure under the two treatment conditions.A total of 31 and 43 genes were found related to desiccation and permeability resistance,respectively.And some genes(cysM,thuF,ycjO,etc.)were found to be associated with two tolerances for the first time.
