将木霉制剂作用于番茄根际土壤微生物,利用Biolog技术检测分析了FF微平板中95种碳源的利用程度,并根据24-192 h单孔平均颜色变化率（AWCD）,分析了木霉菌对土壤微生物生理活性的影响,同时鉴定了供试土壤中的真菌种类.结果表明,木霉制剂...将木霉制剂作用于番茄根际土壤微生物,利用Biolog技术检测分析了FF微平板中95种碳源的利用程度,并根据24-192 h单孔平均颜色变化率（AWCD）,分析了木霉菌对土壤微生物生理活性的影响,同时鉴定了供试土壤中的真菌种类.结果表明,木霉制剂可在番茄根际土壤中定置并生长繁殖,能有效抑制土壤中病原真菌（厚孢镰刀霉厚孢变种Fusariumchlamydosporum var. chlamydosporum,球孢枝孢霉Cladosporiumsphaerospermum,葡萄状单隔霉Ulocladiumbotrytis）的生长,降低土壤中有害生物的生理活性,改变土壤微生物循环,从而达到防治番茄土传病害的目的.展开更多
As one of the main methods of microbial community functional diversity measurement, biolog method was favored by many researchers for its simple oper- ation, high sensitivity, strong resolution and rich data. But the ...As one of the main methods of microbial community functional diversity measurement, biolog method was favored by many researchers for its simple oper- ation, high sensitivity, strong resolution and rich data. But the preprocessing meth- ods reported in the literatures were not the same. In order to screen the best pre- processing method, this paper took three typical treatments to explore the effect of different preprocessing methods on soil microbial community functional diversity. The results showed that, method B＇s overall trend of AWCD values was better than A and C＇s. Method B＇s microbial utilization of six carbon sources was higher, and the result was relatively stable. The Simpson index, Shannon richness index and Car- bon source utilization richness index of the two treatments were B〉C〉A, while the Mclntosh index and Shannon evenness were not very stable, but the difference of variance analysis was not significant, and the method B was always with a smallest variance. Method B＇s principal component analysis was better than A and C＇s. In a word, the method using 250 r/min shaking for 30 minutes and cultivating at 28 ℃ was the best one, because it was simple, convenient, and with good repeatability.展开更多
文摘将木霉制剂作用于番茄根际土壤微生物,利用Biolog技术检测分析了FF微平板中95种碳源的利用程度,并根据24-192 h单孔平均颜色变化率（AWCD）,分析了木霉菌对土壤微生物生理活性的影响,同时鉴定了供试土壤中的真菌种类.结果表明,木霉制剂可在番茄根际土壤中定置并生长繁殖,能有效抑制土壤中病原真菌（厚孢镰刀霉厚孢变种Fusariumchlamydosporum var. chlamydosporum,球孢枝孢霉Cladosporiumsphaerospermum,葡萄状单隔霉Ulocladiumbotrytis）的生长,降低土壤中有害生物的生理活性,改变土壤微生物循环,从而达到防治番茄土传病害的目的.
基金Supported by National and International Scientific and Technological Cooperation Project"The application of Microbial Agents on Mining Reclamation and Ecological Recovery"(2011DFR31230)Key Project of Shanxi academy of Agricultural Science"The Research and Application of Bio-organic Fertilizer on Mining Reclamation and Soil Remediation"(2013zd12)Major Science and Technology Programs of Shanxi Province"Key Technology Research and Demonstration of mining waste land ecosystem Restoration and Reconstruction"(20121101009)~~
文摘As one of the main methods of microbial community functional diversity measurement, biolog method was favored by many researchers for its simple oper- ation, high sensitivity, strong resolution and rich data. But the preprocessing meth- ods reported in the literatures were not the same. In order to screen the best pre- processing method, this paper took three typical treatments to explore the effect of different preprocessing methods on soil microbial community functional diversity. The results showed that, method B＇s overall trend of AWCD values was better than A and C＇s. Method B＇s microbial utilization of six carbon sources was higher, and the result was relatively stable. The Simpson index, Shannon richness index and Car- bon source utilization richness index of the two treatments were B〉C〉A, while the Mclntosh index and Shannon evenness were not very stable, but the difference of variance analysis was not significant, and the method B was always with a smallest variance. Method B＇s principal component analysis was better than A and C＇s. In a word, the method using 250 r/min shaking for 30 minutes and cultivating at 28 ℃ was the best one, because it was simple, convenient, and with good repeatability.
