[Objective] The aim was to summarize the artificial reproduction and fry rearing technique of Cichlasoma managuense. [Method] The artificial reproduction and fry rearing technique of Cichlasoma managuense were carried...[Objective] The aim was to summarize the artificial reproduction and fry rearing technique of Cichlasoma managuense. [Method] The artificial reproduction and fry rearing technique of Cichlasoma managuense were carried out. [ Result] Two years old parent fish which cultured in nest can pair and spawn by themselves. The absolute fecundity of female fish were 1 500 -2 500 eggs/fish, the relative fecundity were 500 -800 eggs/fish. Fertilized eggs were elliptic, dark yellow, opaque, viscous, and arranged dispersedly in the nest. 50 of 72 pairs of parent fish were matched and spawned. The spawn rate was 74.2% and the fertility rate was 92.6% during the trial period. The time from fertilized egg to larva was 48 h ~ 41 min, Total body length of larva was 0.65 ±0.35 cm, hatching rate was 84.8%, total initial hatched fry was 401 000 in the water temperature 27 -30 ℃[ Conclusion] 322 000 fishes of 3.0 -5.0 cm fry were obtained in the cement pool with small cages cultivation in 41 -43 d, survival rate 78.5%.展开更多
Background MicroRNAs(miRNAs)are presented in the uterine lumen of many mammals,and in vitro experiments have determined that several miRNAs are important for the regulation of endometrial and trophoblast functions.Our...Background MicroRNAs(miRNAs)are presented in the uterine lumen of many mammals,and in vitro experiments have determined that several miRNAs are important for the regulation of endometrial and trophoblast functions.Our aim was to identify and contrast the miRNAs present in extracellular vesicles(EVs)in the uterine lumen fluid(ULF)at the onset of attachment in cattle pregnancies(gestation d 18)initiated by artificial insemination(AI)or by the trans-fer of an in vitro-produced blastocyst(IVP-ET).A third group had no conceptus after the transfer of an IVP embryo.Results The abundance of 263 annotated miRNAs was quantified in the EVs collected from ULF.There was an increase in the transcript abundance of 20 miRNAs in the ULF EVs from the AI pregnant group,while 4 miRNAs had a lower abundance relative to the group not containing a conceptus.Additionally,4 miRNAs were more abun-dant in ULF EVs in the AI pregnant group relative to IVP-ET group(bta-mir-17,bta-mir-7-3,MIR7-1,MIR18A).Specific miRNAs in the ULF EVs were co-expressed with messenger RNAs expressed in extra-embryonic tissues and endome-trium,including genes that are known to be their targets.Conclusions The results provide biological insights into the participation of miRNAs in the regulation of trophoblast proliferation and differentiation,as well as in endometrium receptivity.The knowledge that in vitro cultured embryos can contribute to the altered abundance of specific miRNAs in the uterine lumen can lead to the development of cor-rective approaches to reduce conceptus losses during the first month of pregnancy in cattle.展开更多
基金funded by Hainan Province Key Scientific and Technological Projects(ZDXM20110028)Sanya Key Scientific and Technological Projects(2010yf09)
文摘[Objective] The aim was to summarize the artificial reproduction and fry rearing technique of Cichlasoma managuense. [Method] The artificial reproduction and fry rearing technique of Cichlasoma managuense were carried out. [ Result] Two years old parent fish which cultured in nest can pair and spawn by themselves. The absolute fecundity of female fish were 1 500 -2 500 eggs/fish, the relative fecundity were 500 -800 eggs/fish. Fertilized eggs were elliptic, dark yellow, opaque, viscous, and arranged dispersedly in the nest. 50 of 72 pairs of parent fish were matched and spawned. The spawn rate was 74.2% and the fertility rate was 92.6% during the trial period. The time from fertilized egg to larva was 48 h ~ 41 min, Total body length of larva was 0.65 ±0.35 cm, hatching rate was 84.8%, total initial hatched fry was 401 000 in the water temperature 27 -30 ℃[ Conclusion] 322 000 fishes of 3.0 -5.0 cm fry were obtained in the cement pool with small cages cultivation in 41 -43 d, survival rate 78.5%.
基金Agriculture and Food Research Initiative Competitive Grant no.2018-67015-31936 from the USDA National Institute of Food and Agriculture.
文摘Background MicroRNAs(miRNAs)are presented in the uterine lumen of many mammals,and in vitro experiments have determined that several miRNAs are important for the regulation of endometrial and trophoblast functions.Our aim was to identify and contrast the miRNAs present in extracellular vesicles(EVs)in the uterine lumen fluid(ULF)at the onset of attachment in cattle pregnancies(gestation d 18)initiated by artificial insemination(AI)or by the trans-fer of an in vitro-produced blastocyst(IVP-ET).A third group had no conceptus after the transfer of an IVP embryo.Results The abundance of 263 annotated miRNAs was quantified in the EVs collected from ULF.There was an increase in the transcript abundance of 20 miRNAs in the ULF EVs from the AI pregnant group,while 4 miRNAs had a lower abundance relative to the group not containing a conceptus.Additionally,4 miRNAs were more abun-dant in ULF EVs in the AI pregnant group relative to IVP-ET group(bta-mir-17,bta-mir-7-3,MIR7-1,MIR18A).Specific miRNAs in the ULF EVs were co-expressed with messenger RNAs expressed in extra-embryonic tissues and endome-trium,including genes that are known to be their targets.Conclusions The results provide biological insights into the participation of miRNAs in the regulation of trophoblast proliferation and differentiation,as well as in endometrium receptivity.The knowledge that in vitro cultured embryos can contribute to the altered abundance of specific miRNAs in the uterine lumen can lead to the development of cor-rective approaches to reduce conceptus losses during the first month of pregnancy in cattle.
