This study aimed to reveal how amoxicillin（AMX） affected the microbial community and the spread mechanism of antibiotic resistance genes（ARGs） in the AMX manufacture wastewater treatment system. For this purpose, ...This study aimed to reveal how amoxicillin（AMX） affected the microbial community and the spread mechanism of antibiotic resistance genes（ARGs） in the AMX manufacture wastewater treatment system. For this purpose, a 1.47 L expanded granular sludge bed（EGSB） reactor was designed and run for 241 days treating artificial AMX manufacture wastewater. 454 pyrosequencing was applied to analyze functional microorganisms in the system. The antibiotic genes OXA_（-1）, OXA_（-2）, OXA_（-10）, TEM_（-1）, CTX-M_（-1）, class I integrons（intI1） and 16 SrRNA genes were also examined in sludge samples. The results showed that the genera Ignavibacterium, Phocoenobacter,Spirochaeta, Aminobacterium and Cloacibacillus contributed to the degradation of different organic compounds（such as various sugars and amines）. And the relative quantification of eachβ-lactam resistance gene in the study was changed with the increasing of AMX concentration.Furthermore the vertical gene transfer was the main driver for the spread of ARGs rather than horizontal transfer pathways in the system.展开更多
The growing interest in the anaerobic ammonium oxidizing(AMX)process in treating high nitrogen containing wastewaters and a comprehensive study into the granulation mechanism of these bacteria under diverse environmen...The growing interest in the anaerobic ammonium oxidizing(AMX)process in treating high nitrogen containing wastewaters and a comprehensive study into the granulation mechanism of these bacteria under diverse environmental conditions over the years have been unequal.To this effect,the distinctive differences in saline adapted AMX(S_AMX)and nonsaline adapted AMX(NS_AMX)granules are presented in this study.It was observed that substrate utilisation profiles,granule formation mechanism,and pace towards granulation differed marginally for the two adaptation conditions.The different microbial dominant aggregation types aided in splitting the 471 days operated lab-scale SBRs into three distinct phases.In both reactors,phase III(granules dominant phase)showed the highest average nitrogen removal efficiency of 87.9%±4.8%and 85.6%±3.6%for the S_AMX and NS_AMX processes,respectively.The extracellular polymeric substances(EPS)quantity and major composition determined its role either as a binding agent in granulation or a survival mechanism in saline adaptation.It was also observed that granules of the S_AMX reactor were mostly loosely and less condensed aggregates of smaller sub-units and flocs while those of the NS_AMX reactor were compact agglomerates.The ionic gradient in saline enrichment led to an increased activity of the Na^(+)/K^(+)–ATPase,hence enriched granules produced higher cellular adenosine triphosphate molecules which finally improved the granules active biomass ratio by 32.96%.Microbial community showed that about three to four major known AMX species made up the granules consortia in both reactors.Proteins and expression of functional genes differed for these different species.展开更多
2020年11月,安慕希推出健康子品牌AMX。“AMX”取自安慕希拼音首字母,同时代表着Ai Mao Xian,品牌主张“新一代爱冒险”的价值理念,鼓励年轻一代大胆破新、迎接挑战!安慕希AMX定位新一代健康酸奶,先后推出基础健康系列、健康轻美味系列...2020年11月,安慕希推出健康子品牌AMX。“AMX”取自安慕希拼音首字母,同时代表着Ai Mao Xian,品牌主张“新一代爱冒险”的价值理念,鼓励年轻一代大胆破新、迎接挑战!安慕希AMX定位新一代健康酸奶,先后推出基础健康系列、健康轻美味系列和健康轻功能系列产品。2021年上市首年AMX销售额突破十亿,其中小黑钻、小黑瓶以“0蔗糖,低GI”为核心卖点,表现优异;2022年AMX推出产地直采系列丹东草莓和新疆哈密瓜,双品销售额突破十亿,引领常温酸奶行业。展开更多
β-lactam antibiotics in aquatic environment have severely damaged ecological stability and caused a series of environmental pollution problems to be solved urgently.Herein,a novel composite photocatalyst prepared by ...β-lactam antibiotics in aquatic environment have severely damaged ecological stability and caused a series of environmental pollution problems to be solved urgently.Herein,a novel composite photocatalyst prepared by loading carbon dots(CDs)onto rod-like CoFe_(2)O_(4)(CFO),which can effectively degrade amoxicillin(AMX)by photocata lytic/peroxy mono sulfate(PMS)activation under visible light irradiation.The degradation results exhibits that the optimal degradation efficiency with 97.5%within 80 min is achievd by the CDs-CFO-5 composite.Such enhanced activity is ascribed to the introduction of CDs that effectively improves the separation efficiency of photogenerated electron pairs and creates new active sites as electron bridges that improve the photocata lytic performance.More importantly,a strong synergistic between CDs and photo-induced electrons generated from CFO can further activiate PMS to provide more SO4-·and·OH radicals for boosting the degradation ability towards AMX.The present study aims to elucidate positive role of CDs in photocata lytic/peroxy monosulfate activation during the degradation reaction.展开更多
基金supported by the National Natural Science Foundation of China (No.51478138)the State Key Lab of Urban Water Resource and Environment (No.HIT ES200902)
文摘This study aimed to reveal how amoxicillin（AMX） affected the microbial community and the spread mechanism of antibiotic resistance genes（ARGs） in the AMX manufacture wastewater treatment system. For this purpose, a 1.47 L expanded granular sludge bed（EGSB） reactor was designed and run for 241 days treating artificial AMX manufacture wastewater. 454 pyrosequencing was applied to analyze functional microorganisms in the system. The antibiotic genes OXA_（-1）, OXA_（-2）, OXA_（-10）, TEM_（-1）, CTX-M_（-1）, class I integrons（intI1） and 16 SrRNA genes were also examined in sludge samples. The results showed that the genera Ignavibacterium, Phocoenobacter,Spirochaeta, Aminobacterium and Cloacibacillus contributed to the degradation of different organic compounds（such as various sugars and amines）. And the relative quantification of eachβ-lactam resistance gene in the study was changed with the increasing of AMX concentration.Furthermore the vertical gene transfer was the main driver for the spread of ARGs rather than horizontal transfer pathways in the system.
基金supported by the National Research Foundation(NRF)of Korea(project#:NRF-2020R1A2C2006769)。
文摘The growing interest in the anaerobic ammonium oxidizing(AMX)process in treating high nitrogen containing wastewaters and a comprehensive study into the granulation mechanism of these bacteria under diverse environmental conditions over the years have been unequal.To this effect,the distinctive differences in saline adapted AMX(S_AMX)and nonsaline adapted AMX(NS_AMX)granules are presented in this study.It was observed that substrate utilisation profiles,granule formation mechanism,and pace towards granulation differed marginally for the two adaptation conditions.The different microbial dominant aggregation types aided in splitting the 471 days operated lab-scale SBRs into three distinct phases.In both reactors,phase III(granules dominant phase)showed the highest average nitrogen removal efficiency of 87.9%±4.8%and 85.6%±3.6%for the S_AMX and NS_AMX processes,respectively.The extracellular polymeric substances(EPS)quantity and major composition determined its role either as a binding agent in granulation or a survival mechanism in saline adaptation.It was also observed that granules of the S_AMX reactor were mostly loosely and less condensed aggregates of smaller sub-units and flocs while those of the NS_AMX reactor were compact agglomerates.The ionic gradient in saline enrichment led to an increased activity of the Na^(+)/K^(+)–ATPase,hence enriched granules produced higher cellular adenosine triphosphate molecules which finally improved the granules active biomass ratio by 32.96%.Microbial community showed that about three to four major known AMX species made up the granules consortia in both reactors.Proteins and expression of functional genes differed for these different species.
文摘2020年11月,安慕希推出健康子品牌AMX。“AMX”取自安慕希拼音首字母,同时代表着Ai Mao Xian,品牌主张“新一代爱冒险”的价值理念,鼓励年轻一代大胆破新、迎接挑战!安慕希AMX定位新一代健康酸奶,先后推出基础健康系列、健康轻美味系列和健康轻功能系列产品。2021年上市首年AMX销售额突破十亿,其中小黑钻、小黑瓶以“0蔗糖,低GI”为核心卖点,表现优异;2022年AMX推出产地直采系列丹东草莓和新疆哈密瓜,双品销售额突破十亿,引领常温酸奶行业。
基金founding support from the National Natural Science Foundation of China(Nos.21906072,22006057 and 31971616)the Natural Science Foundation of Jiangsu Province(BK20190982)+4 种基金“Doctor of Mass Entrepreneurship and Innovation”Project in Jiangsu Province,Henan Postdoctoral Foundation(202003013)Doctoral Scientific Research Foundation of Jiangsu University of Science and Technology(China)(1062931806 and 1142931803)the Science and Technology Research Project of the Department of Education of Jilin Province(JJKH20200039KJ)the Science and Technology Research Project of Jilin City(20190104120,201830811)the Project of Jilin Provincial Science and Technology Development Plan(20190201277JC,20200301046RQ,YDZJ202101ZYTS070)。
文摘β-lactam antibiotics in aquatic environment have severely damaged ecological stability and caused a series of environmental pollution problems to be solved urgently.Herein,a novel composite photocatalyst prepared by loading carbon dots(CDs)onto rod-like CoFe_(2)O_(4)(CFO),which can effectively degrade amoxicillin(AMX)by photocata lytic/peroxy mono sulfate(PMS)activation under visible light irradiation.The degradation results exhibits that the optimal degradation efficiency with 97.5%within 80 min is achievd by the CDs-CFO-5 composite.Such enhanced activity is ascribed to the introduction of CDs that effectively improves the separation efficiency of photogenerated electron pairs and creates new active sites as electron bridges that improve the photocata lytic performance.More importantly,a strong synergistic between CDs and photo-induced electrons generated from CFO can further activiate PMS to provide more SO4-·and·OH radicals for boosting the degradation ability towards AMX.The present study aims to elucidate positive role of CDs in photocata lytic/peroxy monosulfate activation during the degradation reaction.
