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Science Letters:Quantification of Helicobacter pylori levels in soil samples from public playgrounds in Spain 被引量:2
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作者 Leonardo Martín PéREZ Francesc CODONY +3 位作者 Daniela LóPEZ LEYTON Mariana FITTIPALDI Bárbara ADRADOS Jordi MORATó 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2010年第1期27-29,共3页
Helicobacter pylori are ubiquitous Gram-negative bacteria with a high estimated level of infection in the world populations, but a majority of the infected persons are asymptomatic. This pathogen has been classified b... Helicobacter pylori are ubiquitous Gram-negative bacteria with a high estimated level of infection in the world populations, but a majority of the infected persons are asymptomatic. This pathogen has been classified by the World Health Organization as a class I carcinogen and recognized as the causal agent of most peptic ulcers and chronic gastritis that might lead to stomach cancer. Although not all the transmission pathways of these bacteria into humans have been propedy identified, enough data have suggested that the oral-oral or fecal-oral ones are the main infection routes. Helicobacterpylori have been detected in non-treated water and in drinking water, which suggested that water might be an important infection source. As childhood is the critical period of infection, the aim of the present work was to examine the presence of Helicobacterpylori in soil samples from public playing areas of Spanish parks. 展开更多
关键词 Heficobacterpylori Real time polymerase chain reaction (qPCR) Environmental soil samples
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New Series of Soil Samplers and Their Application
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作者 JIASHU-GANG YANGXUE-MING 《Pedosphere》 SCIE CAS CSCD 1995年第2期179-182,共4页
In this paper,the structure of 'double juts' soil sampler and a series of new soil samplers are introduced.The internal diameter of auger tip the sampler is less than that of sampling tube, but the external di... In this paper,the structure of 'double juts' soil sampler and a series of new soil samplers are introduced.The internal diameter of auger tip the sampler is less than that of sampling tube, but the external diameterof auger tip is larger than that of sampling tube. Therefore, adhesion and cutting resistance can be reducedby limiting connection areas between sampler and the soil. Such a new structure makes it possible to bewidely used for the production of a series of special soil samplers. 展开更多
关键词 cutting ring handoperated soil sampler sampling topsoil sampler
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Complex Effective Relative Permittivity of Soil Samples from the Taunus Region (Germany) 被引量:1
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作者 Katja Lauer Christian Albrecht +1 位作者 Christina Salat Peter Felix-Henningsen 《Journal of Earth Science》 SCIE CAS CSCD 2010年第6期961-967,共7页
The most important parameter affecting ground-penetrating radar (GPR) measurements is the complex effective relative permittivity εr^*,eff because it controls the propagation velocity and the reflection of GPR pul... The most important parameter affecting ground-penetrating radar (GPR) measurements is the complex effective relative permittivity εr^*,eff because it controls the propagation velocity and the reflection of GPR pulses. Knowing εr^*,eff of soils passed through by electromagnetic waves increases accuracy in soil thickness and interface identification. Complex effective relative permittivity εr^*,eff= εr^*,eff - jεr^*,effof 25 soil samples with textures ranging from loamy sand to silty clay was measured using the two-electrode parallelplate method. The measurements were conducted at defined water contents for frequencies from 1 MHz to 3 GHz. The results confirm the frequency dependence of εr^*,eff and show that the dielectric behavior of soil-water mixtures is a function of water content. Applying the experimental data of this study with predictions based on the empirical model by Toppet aL (1980), we find that Topp et aL's curve tends to underestimate the real part of εr^*,eff measured. Along with frequency and water content, soil texture and organic matter affect soil permittivity. Moreover, the real part of εr^*,eff increases at higher dry bulk densities. Output from our calibration model enables us to predict εr^*,eff for the soil samples which were tested under the actual in situ soil water content. This results in high accuracy of soil thickness prediction. 展开更多
关键词 ground-penetrating radar (GPR) complex effective relative permittivity soil sample.
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Discussion on Atmospheric Pollutant Source Seeking Model with Surface Soil Sample
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作者 Lv Ning Lin Hongtao 《Meteorological and Environmental Research》 CAS 2014年第3期4-6,共3页
Through the analysis on the migratory diffusion process of atmospheric pollutants,we proposed to seek atmospheric pollutant source with surface soil sample of data.Based on Gaussian plume model and deposition model,at... Through the analysis on the migratory diffusion process of atmospheric pollutants,we proposed to seek atmospheric pollutant source with surface soil sample of data.Based on Gaussian plume model and deposition model,atmospheric pollutants distribution model was deduced,with which a schema matching source seeking model was established.The model was used to seek the pollutant source by using the arsenic data in the surface soil sample of a city. 展开更多
关键词 Atmospheric pollutant source soil sample Source seeking model Parameter fitting China
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Screening and Characterization of Antibiotic Producing Organisms from Waste Dump Soil Sample
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作者 Chinenye U. Ezebialu Ikechukwu B. Awuribeh +3 位作者 Ebere M. Eze Chigoziri T. Ogu Uchechukwu G. Nwankwo Ruth Asikiya Afunwa 《Advances in Microbiology》 2020年第9期422-433,共12页
This study was designed to screen and characterize antibiotic producing bacteria and fungi from soil samples from a waste dump site in Enugu. The molecular characterization of the isolates was performed by using Zymo ... This study was designed to screen and characterize antibiotic producing bacteria and fungi from soil samples from a waste dump site in Enugu. The molecular characterization of the isolates was performed by using Zymo Research Quick-DNA fungi/bacteria miniprep kit. The bacteria isolates were <i>Providencia stuartii</i>, <i>Providencia alcalifaciens.</i> <i>Bacillus sp.</i>, <i>Streptococcus sp.</i>, <i>Micrococcus sp.</i>, and <i>Staphylococcus sp</i> while <i>Aspergillus fumigatus</i> and <i>Aspergillus niger</i> were fungal isolates. The efficacy of the Crude Extract (CE) of the antibiotics produced was tested against species of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. The CE of antibiotics when compared with a conventional antibiotic (Ciprofloxacin) showed lower antimicrobial activity. The minimum inhibitory concentration of the crude extracts ranged between 12.5% and 25% concentrations while the minimum bactericidal concentration ranged from 25% and 50% concentrations. The findings suggest that these organisms have the potential to produce antibiotics and could be harnessed by pharmaceutical industries for the production of newer antibiotics. 展开更多
关键词 ANTIBIOTICS Crude Extract CIPROFLOXACIN soil sample
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A Highly Sensitive and Selective Spectrofluorimetric Method for the Determination of Arsenic at Pico-Trace Levels in Some Groundwater, Real, Environmental, Biological, Food and Soil Samples Using 2-(<i>α</i>-Pyridyl)-Thioquinaldinamide
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作者 M. Jamaluddin Ahmed Ayesha Afrin Mamunur Rashid 《American Journal of Analytical Chemistry》 2019年第8期316-347,共32页
A very simple, ultra-sensitive, highly selective and non-extractive new spectrofluorimetric method for the determination of arsenic at pico-trace levels using 2-(α-pyridyl)-thioquinaldinamide (PTQA) has been develope... A very simple, ultra-sensitive, highly selective and non-extractive new spectrofluorimetric method for the determination of arsenic at pico-trace levels using 2-(α-pyridyl)-thioquinaldinamide (PTQA) has been developed. PTQA has been proposed as a new analytical reagent for the direct non-extractive spectrofluorimetric determination of Arsenic (V). This novel fluorimetric reagent, PTQA becomes oxidized in a slightly acidic (0.025 - 0.1 M H2SO4) solution with Arsenic (V) in absolute ethanol to produce highly fluorescent oxidized product (λex = 303 nm;λem = 365 nm). Constant and maximum fluorescence intensities were observed over a wide range of acidity (0.025 - 0.1 M H2SO4) for the period between 2 min and 24 h. Linear calibration graphs were obtained for 0.001 - 800-μgL-1 of As, having a detection limit of 0.1-ngL-1;the quantification limit of the reaction system was found to be 1-ngL-1 and the RSD was 0% - 2%. A large excess of over 60 cations, anions and complexion agents (like, chloride, phosphate, azide, tartrate, oxalate, SCN, etc.) do not interfere in the determination. The developed method was successfully used in the determination of arsenic in several Certified Reference Materials (alloys, steels, ores, human urine, hair, nails, bovine liver and sediments) as well as in some biological fluids (human blood, urine, hair, nail and milk), soil samples, food samples (vegetables, fruits, rice, corn and wheat), solutions containing both arsenic (III) and arsenic (V) speciation and complex synthetic mixtures. The results of the proposed method for assessing biological, food and soil samples were comparable with both ICP-OES & AHG-AAS and were found to be in excellent agreement. 展开更多
关键词 Spectrofluorimetry ARSENIC Determination GROUNDWATER 2-(α-Pyridyl)-thioquinaldinamide ENVIRONMENTAL BIOLOGICAL soil FOOD samples
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悬浮液HG-AFS测定海洋沉积物样品中痕量砷 被引量:3
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作者 程祥圣 秦晓光 +3 位作者 刘富平 杨颖 徐韧 苏耀东 《理化检验(化学分册)》 CAS CSCD 北大核心 2003年第9期508-511,共4页
研究了一种使用悬浮液进样原子荧光光谱法测定海洋沉积物中痕量砷的快速简便的方法。控制悬浮液的颗粒粒径在160目(d≤0 088mm),用磁力搅拌器搅拌以保证悬浮液分散、均匀、稳定,试验了悬浮液的粒度、介质、酸度、悬浮液浓度等因素的影响... 研究了一种使用悬浮液进样原子荧光光谱法测定海洋沉积物中痕量砷的快速简便的方法。控制悬浮液的颗粒粒径在160目(d≤0 088mm),用磁力搅拌器搅拌以保证悬浮液分散、均匀、稳定,试验了悬浮液的粒度、介质、酸度、悬浮液浓度等因素的影响,采用水溶液标准曲线直接进行校准。该方法的检出限为0.025μg·g-1,加标回收率为85.6%~109.0%,RSD为2.19%。用标准物质对方法进行考核,结果满意。 展开更多
关键词 悬浮液 HG-afs 测定 海洋沉积物 样品 痕量元素 原子荧光光谱法
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HG-AFS法测定环境水样中痕量铅 被引量:2
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作者 倪刚 宋伟民 +2 位作者 卓琳 吴月 胡奇林 《宁夏工程技术》 CAS 2003年第1期47-49,共3页
建立了磷酸介质中氢化物-原子荧光光谱法(HG-AFS)测定环境水样中铅的新方法.研究了酸介质、氢化物发生、掩蔽剂等因素对测定的影响,并选择出仪器的最佳工作条件.成功地应用于在较宽范围内对环境水样中铅含量进行快速、准确、灵敏地测定.... 建立了磷酸介质中氢化物-原子荧光光谱法(HG-AFS)测定环境水样中铅的新方法.研究了酸介质、氢化物发生、掩蔽剂等因素对测定的影响,并选择出仪器的最佳工作条件.成功地应用于在较宽范围内对环境水样中铅含量进行快速、准确、灵敏地测定.在0.5%磷酸介质中,采用4g/L草酸溶液来掩蔽相关离子的干扰,可以检测0~300μg/L范围内的铅,检出限为0.84μg/L,能满足环境水样中铅含量的测定要求,相对标准偏差(RSD)在5%以内,加标回收率在96%~104%之间,结果令人满意. 展开更多
关键词 HG-afs 氢化物-原子荧光光谱法 环境水样 环境监测 含量测定 酸介质 掩蔽剂
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HG-AFS法测定地质样品中微量硒的不确定度评定
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作者 李林庆 《中国测试》 CAS 北大核心 2013年第S2期21-24,共4页
通过氢化物发生-原子荧光光谱法(HG-AFS)对地质样品中的硒进行测定,对测定结果的不确定度来源进行分析,评定不确定度的分量。结果表明,该方法测量不确定度引入的最主要来源有两项:硒标准溶液配制过程和重复性测量样品。因此在实际分析... 通过氢化物发生-原子荧光光谱法(HG-AFS)对地质样品中的硒进行测定,对测定结果的不确定度来源进行分析,评定不确定度的分量。结果表明,该方法测量不确定度引入的最主要来源有两项:硒标准溶液配制过程和重复性测量样品。因此在实际分析过程中,应提高分析人员检测技能和规范操作,保证测定结果的准确性和可靠性。当测试样品中硒的含量为0.1631μg/g时,其扩展不确定度(k=2)为0.0081μg/g。 展开更多
关键词 HG-afs 不确定度评定 地质样品
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HG-AFS测定地质样品中的总硒 被引量:2
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作者 马真真 郭宇 +2 位作者 鲍征宇 李慧 魏昌华 《光谱实验室》 CAS CSCD 2012年第5期2757-2760,共4页
采用王水消解法处理样品,用氢化物发生-原子荧光光谱法测定土壤中总硒的含量,取得了较满意的效果,并与硝酸-高氯酸电热板消解法进行了对比分析,得出结论:王水消解法不仅操作简单、省时,而且总硒测定的准确度也高于硝酸-高氯酸电热板消解... 采用王水消解法处理样品,用氢化物发生-原子荧光光谱法测定土壤中总硒的含量,取得了较满意的效果,并与硝酸-高氯酸电热板消解法进行了对比分析,得出结论:王水消解法不仅操作简单、省时,而且总硒测定的准确度也高于硝酸-高氯酸电热板消解法,为大量地质样品中总硒含量的测定提供了一种简便可行的方法。 展开更多
关键词 氢化物发生-原子荧光光谱法 地质样品 总硒
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水相乙基化GC-AFS测定环境及生物样品中甲基汞 被引量:15
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作者 黄卓尔 《分析测试学报》 CAS CSCD 1998年第1期21-25,共5页
介绍基于水相乙基化和气相色谱(GC)-原子荧光(AFS)的甲基汞分析法。水溶液中甲基汞与四乙基硼化钠反应生成甲乙基汞,由氮气吹扫捕集于Tenax管,然后由GC_AFS测定。应用于环境及生物样品,检测限为05pgHg。
关键词 甲基汞 水相乙基化 GC afs 环境样品 生物样品
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微波辅助萃取-HPLC-AFS联用技术检测土壤样品中砷的化学形态 被引量:7
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作者 陈邵鹏 仲雪 《中国环境监测》 CAS CSCD 北大核心 2014年第4期159-162,共4页
建立了微波辅助萃取-高效液相色谱(HPLC)-原子荧光光谱(AFS)联用技术分析土壤中亚砷酸盐[As(Ⅲ)]、二甲基砷( DMA)、一甲基砷( MMA)和砷酸盐[ As(Ⅴ)]等4种形态砷。用正交实验设计优化了功率、萃取溶剂体积、萃取温度、萃... 建立了微波辅助萃取-高效液相色谱(HPLC)-原子荧光光谱(AFS)联用技术分析土壤中亚砷酸盐[As(Ⅲ)]、二甲基砷( DMA)、一甲基砷( MMA)和砷酸盐[ As(Ⅴ)]等4种形态砷。用正交实验设计优化了功率、萃取溶剂体积、萃取温度、萃取时间等微波萃取条件。方法的线性范围为1.00~100μg/L,As(Ⅲ)、DMA、MMA 和As(Ⅴ)的检出限分别为0.21、0.33、0.38、0.56μg/L,相对标准偏差为4.6%~5.5%,样品回收率为78.1%~94.4%。用该法分析了4种土壤样品中的砷形态。 展开更多
关键词 微波辅助萃取 土壤 形态分析
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采用HG-AFS结合XRF测定土壤中的砷 被引量:3
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作者 王龙 任冬 +1 位作者 武应凯 张廷忠 《世界有色金属》 2019年第2期253-257,共5页
为了快速准确有效的分析土壤中砷的含量.更好地为环境管理服务,保障人民群众生活环境健康。采用氢化物发生-原子荧光光谱仪(HG-AFS)结合X荧光光谱仪(XRF)测定土壤中的砷,测定结果中AFS精密度(RSD)为2.3%~5.5%,XRF精密度(RSD)为3.5%~8.... 为了快速准确有效的分析土壤中砷的含量.更好地为环境管理服务,保障人民群众生活环境健康。采用氢化物发生-原子荧光光谱仪(HG-AFS)结合X荧光光谱仪(XRF)测定土壤中的砷,测定结果中AFS精密度(RSD)为2.3%~5.5%,XRF精密度(RSD)为3.5%~8.6%;AFS准确度(RE)为-2.3%~5.4%,XRF准确度(RE)为-3.9%~7.9%,方法检出限为0.018μg/g,其质量满足规范要求。与HJ 680-2013做方法比对,数据一致性好,可靠。其充分解决了土壤中砷含量在0.2μg/g~600μg/g范围内样品检测分析存在干扰的问题,可以作为土壤中砷检测的一种补充方法,具有一定的应用推广价值。 展开更多
关键词 环境 土壤 污染 HG-afs XRF
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ICP-AES/AFS联合测定金矿地质样品中32种主次痕量元素 被引量:3
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作者 赵刚 谢璐 +1 位作者 龙军桥 邱宏喜 《黄金》 CAS 2015年第1期70-74,共5页
建立了电感耦合等离子体发射光谱(ICP-AES)与原子荧光光谱(AFS)联合测定金矿地质样品中32种主、次、微痕量元素的方法。样品分别采用HNO3-HF-HCl O4-H3PO4和KOH及含酒石酸的王水分解,ICP-AES依次测定主量元素Si、Al、Ca、Mg、Fe、P,较... 建立了电感耦合等离子体发射光谱(ICP-AES)与原子荧光光谱(AFS)联合测定金矿地质样品中32种主、次、微痕量元素的方法。样品分别采用HNO3-HF-HCl O4-H3PO4和KOH及含酒石酸的王水分解,ICP-AES依次测定主量元素Si、Al、Ca、Mg、Fe、P,较高含量元素Mo、Sn、W,以及Ag、As、B、Ba、Be、Bi、Ce、Co、Cr、Cu、K、Mn、Na、Sb、Sr、Ti、Ni、Pb、V、Zn等;AFS依次测定微痕量Hg和Se、Te。该方法应用于金矿地质样品和多金属国家标准样品分析,测定结果与推荐值吻合,精密度(RSD)小于10%,为金矿地质样品化学组成和多金属矿床成矿环境、成因机制的研究以及矿产资源的综合利用等,提供了充分的依据。 展开更多
关键词 电感耦合等离子体发射光谱法 原子荧光光谱法 地质样品 主次量元素 微痕量元素
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HCL-ICP-AFS测定地质样品中锂、钠、钾、铷 被引量:3
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作者 王芹香 《分析试验室》 CAS CSCD 北大核心 1992年第2期75-77,共3页
本文用HCL-ICP-AFS方法测地质样品中Li、Na、K、Rb。着重研究了各种酸介质、浓度及其共存元素对测定的影响。在本文实验条件下它们的检出限(DL)为:Li,1.8ppb;Na,0.34ppb;K,0.92ppb;Rb,2.7ppb。对GSD-10标样分别取样测量11次,标准偏差为:... 本文用HCL-ICP-AFS方法测地质样品中Li、Na、K、Rb。着重研究了各种酸介质、浓度及其共存元素对测定的影响。在本文实验条件下它们的检出限(DL)为:Li,1.8ppb;Na,0.34ppb;K,0.92ppb;Rb,2.7ppb。对GSD-10标样分别取样测量11次,标准偏差为:Li,1.2%;Rb,2.2%;K_2O,1.2%;Na_2O,1.5%。对土壤、水系沉积物、岩石类标样测定结果与推荐值吻合。 展开更多
关键词 ICP-afs 地质样品
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HG-AFS法测定土壤样中硒的不确定度评定 被引量:2
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作者 简红霞 刘梯楼 《安徽农业科学》 CAS 2012年第7期4217-4219,共3页
[目的]对测量土壤样品中硒的不确定度进行评定。[方法]采用氢化物发生-原子荧光光谱法(HG-AFS)对土壤样品中的硒进行测定,根据《测量不确定度评定与表示》对其不确定度进行分析。[结果]测量不确定度主要来源于标准溶液配制、曲线拟合、... [目的]对测量土壤样品中硒的不确定度进行评定。[方法]采用氢化物发生-原子荧光光谱法(HG-AFS)对土壤样品中的硒进行测定,根据《测量不确定度评定与表示》对其不确定度进行分析。[结果]测量不确定度主要来源于标准溶液配制、曲线拟合、样品制备、重复测量,并且得出标准不确定度和扩展不确定度。[结论]当土壤中硒的含量为0.642 mg/kg时,其扩展不确定度为0.036 mg/kg(k=2)。 展开更多
关键词 HG-afs 不确定度 土壤
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Erratum to:Interpretable artificial intelligence approach for understanding shear strength in stabilized clay soils using real field soil samples
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作者 Mohamed Noureddin Aghyad Al Kabbani +1 位作者 Alejandra Lopez Leena Korkiala-Tanttu 《Frontiers of Structural and Civil Engineering》 2025年第12期2155-2155,共1页
Erratum to 19(5):760-781 Frontiers ofStructural and Civil Engineering 2025,https://doi.org/10.1007/s11709-025-1168-5 The article“Interpretable artificial intelligence approach for understanding shear strength in stab... Erratum to 19(5):760-781 Frontiers ofStructural and Civil Engineering 2025,https://doi.org/10.1007/s11709-025-1168-5 The article“Interpretable artificial intelligence approach for understanding shear strength in stabilized clay soils using real field soil samples”,written by Mohamed Noureddin,Aghyad Al Kabbani,Alejandra Lopez,and Leena Korkiala-Tanttu,was originally published electronically on the publisher's internet portal on May 30,2025 without open access due to an unfortunate oversight during the conversion process. 展开更多
关键词 open access real field soil samples stabilized clay soils interpretable artificial intelligence artificial intelligence approach understanding shear strength shear strength
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海洋生物样品中硒的氢化物发生-原子荧光分析(HG-AFS) 被引量:7
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作者 张朝辉 《青岛海洋大学学报(自然科学版)》 CSCD 北大核心 2001年第3期375-381,共7页
采用硼氢化钠还原法将试样中硒还原为挥发性硒化氢 ,氩为载气将硒化氢吹出并导入火焰原子化器进行原子荧光测定。以正交设计法对该还原反应、氢化物转移、原子化及原子荧光测定条件等进行了详细研究。实验结果表明 ,在佳化条件下 ,该法... 采用硼氢化钠还原法将试样中硒还原为挥发性硒化氢 ,氩为载气将硒化氢吹出并导入火焰原子化器进行原子荧光测定。以正交设计法对该还原反应、氢化物转移、原子化及原子荧光测定条件等进行了详细研究。实验结果表明 ,在佳化条件下 ,该法检出限为 0 .0 72μ g/ dm3,相对标准偏差为 2 .0 %。标准曲线表明 ,在 0 .0 0 0~ 1.0 0 0μ g/ dm3Se浓度范围内 ,有理想的线性关系。本法测定海洋生物样品硒的回收率为 95.8%~ 10 2 .4 %。 展开更多
关键词 氢化物发生-原子荧光法 海洋生物样品 硼氢化钠还原法 回收率
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GF-AAS法和HG-AFS法测定土壤中铅的分析方法比较 被引量:2
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作者 史军 时岚 《理化检验(化学分册)》 CAS CSCD 北大核心 2011年第4期433-434,共2页
为验证土壤中铅的氢化物发生原子荧光光谱法(HG-AFS)的有效性,对3种土壤标准样品(GSS-2,GSS-3及GSS-7)进行测定,同时用国家标准方法GB/T 17141—1997[石墨炉原子吸收光谱法(GF-AAS)]进行比较测定。试验结果表明:两种方法的测定结果均与... 为验证土壤中铅的氢化物发生原子荧光光谱法(HG-AFS)的有效性,对3种土壤标准样品(GSS-2,GSS-3及GSS-7)进行测定,同时用国家标准方法GB/T 17141—1997[石墨炉原子吸收光谱法(GF-AAS)]进行比较测定。试验结果表明:两种方法的测定结果均与认定值一致。在精密度、回收率及检出限等方面,HG-AFS法均达到与GF-AAS法相当的水平。但HG-AFS法具有操作简单、分析快速等优势。 展开更多
关键词 石墨炉原子吸收光谱 氢化物发生-原子荧光光谱法 土壤
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Storage protocol widely adopted for freshly collected soil samples significantly changes microbial sequencing and analyses
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作者 Zhihao Chen Yonghong Wu +2 位作者 Jan Dolfing Shunyao Zhuang Bruce E.Rittmann 《Science Bulletin》 2025年第24期4113-4116,共4页
Soil microorganisms play globally vital roles in the environment,ecology,and agriculture[1],and have become a research hotspot.Most research in these fields depends on microbial sequencing and analysis[2,3],which shou... Soil microorganisms play globally vital roles in the environment,ecology,and agriculture[1],and have become a research hotspot.Most research in these fields depends on microbial sequencing and analysis[2,3],which should ideally be conducted immediately after sample collection[4].However on-site DNA extraction and sequencing are often impractical.Therefore,freshly collected soil samples must be properly stored for transport before sequencing.While this sounds like a natural operation,proper transport of soil samples has been widely overlooked,challenging the accuracy of all related research. 展开更多
关键词 dna extraction sample collection however storage protocol microbial sequencing soil microorganisms soil samples transport environmental research
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