Objective:To lay a foundation for the research on the targeted therapy of osteosarcoma or the regulatory mechanism of the occurrence and development of osteosarcoma by studying the expression of a disintegrin and meta...Objective:To lay a foundation for the research on the targeted therapy of osteosarcoma or the regulatory mechanism of the occurrence and development of osteosarcoma by studying the expression of a disintegrin and metalloproteinase domain-containing protein 8(ADAM8)and epithelial growth factor receptor(EGFR)in osteosarcoma.Methods:Osteosarcoma tissues,osteochondroma tissues,osteoarthritis tissues and normal bone tissues were selected and included in this research.By use of tissue chips and immunohistochemistry,the expression levels of ADAM8 and EGFR in the previously-mentioned tissues were observed and compared.Spearman’s rank correlation test was used to analyze the correlation of ADAM8 and EGFR and their relationship with clinicopathological factors in these cases.Results:The positive expression levels of ADAM8 in osteosarcoma tissues,osteochondroma tissues,osteoarthritis tissues and normal bone tissues were 80.95%,36.36%,62.86%and 24.14%respectively,the difference was of statistical significance(p<.05);the positive expression levels of EGFR in osteosarcoma tissues,osteochondroma tissues,osteoarthritis tissues and normal bone tissues were 66.67%,9.09%,40.00%and 4.76%respectively,the difference was of statistical significance(p<.05);Spearman’s rank correlation test showed that ADAM8 was positively correlated with EGFR(r=.379,p<.05).Conclusions:Both ADAM8 and EGFR have a much higher expression in osteosarcoma tissues,and the combined detection of two indexes can be considered as a new method in the clinical diagnosis of osteosarcoma.展开更多
ADAM8 has been identified as the fibronectin-cleaving enzyme in human degenerative intervertebral disc(IVD)tissues.^(1)Increase of the active form of ADAM8 and its cleavage product,fibronectin fragment,correlated with...ADAM8 has been identified as the fibronectin-cleaving enzyme in human degenerative intervertebral disc(IVD)tissues.^(1)Increase of the active form of ADAM8 and its cleavage product,fibronectin fragment,correlated with an increased degree of IVD degeneration.1 Furthermore,fibronectin fragments have been shown to accelerate the progression of IVD degeneration.^(2)These findings suggest that ADAM8 may play a role in degenerative disc disease,a clinical problem with tremendous socioeconomic burdens in the United States.展开更多
Proteases are essential for homeostasis,and their primary function is proteolytic in extracellular and intracellular compartments.The deregulation of expression,abundance,and activity of proteases has been related to ...Proteases are essential for homeostasis,and their primary function is proteolytic in extracellular and intracellular compartments.The deregulation of expression,abundance,and activity of proteases has been related to several pathologies,including cancer.This deregulation contributes to their pro-tumorigenic activity since they participate in the degradation of extracellular matrix components and adhesion molecules,and the activation of growth factors.However,some proteases,such as ADAM metallopeptidase with thrombospondin type 1 motif 8 and kallikrein-related peptidases 5 and 10,have emerged as tumor suppressors due to their antitumoral actions in specific cancer contexts.In this article,we discuss the antitumoral effects of ADAM metallopeptidase with thrombospondin type 1 motif 8,kallikrein-related peptidases 5 and 10 that have been described to date,suggesting their potential use as novel biomarkers and therapeutic targets in cancer.展开更多
文摘Objective:To lay a foundation for the research on the targeted therapy of osteosarcoma or the regulatory mechanism of the occurrence and development of osteosarcoma by studying the expression of a disintegrin and metalloproteinase domain-containing protein 8(ADAM8)and epithelial growth factor receptor(EGFR)in osteosarcoma.Methods:Osteosarcoma tissues,osteochondroma tissues,osteoarthritis tissues and normal bone tissues were selected and included in this research.By use of tissue chips and immunohistochemistry,the expression levels of ADAM8 and EGFR in the previously-mentioned tissues were observed and compared.Spearman’s rank correlation test was used to analyze the correlation of ADAM8 and EGFR and their relationship with clinicopathological factors in these cases.Results:The positive expression levels of ADAM8 in osteosarcoma tissues,osteochondroma tissues,osteoarthritis tissues and normal bone tissues were 80.95%,36.36%,62.86%and 24.14%respectively,the difference was of statistical significance(p<.05);the positive expression levels of EGFR in osteosarcoma tissues,osteochondroma tissues,osteoarthritis tissues and normal bone tissues were 66.67%,9.09%,40.00%and 4.76%respectively,the difference was of statistical significance(p<.05);Spearman’s rank correlation test showed that ADAM8 was positively correlated with EGFR(r=.379,p<.05).Conclusions:Both ADAM8 and EGFR have a much higher expression in osteosarcoma tissues,and the combined detection of two indexes can be considered as a new method in the clinical diagnosis of osteosarcoma.
文摘ADAM8 has been identified as the fibronectin-cleaving enzyme in human degenerative intervertebral disc(IVD)tissues.^(1)Increase of the active form of ADAM8 and its cleavage product,fibronectin fragment,correlated with an increased degree of IVD degeneration.1 Furthermore,fibronectin fragments have been shown to accelerate the progression of IVD degeneration.^(2)These findings suggest that ADAM8 may play a role in degenerative disc disease,a clinical problem with tremendous socioeconomic burdens in the United States.
基金Supported by Colegio de Ciencia y Tecnologia de la Universidad Autónoma de la Ciudad de México,No.UACM-CCyT-2025-CON-11.
文摘Proteases are essential for homeostasis,and their primary function is proteolytic in extracellular and intracellular compartments.The deregulation of expression,abundance,and activity of proteases has been related to several pathologies,including cancer.This deregulation contributes to their pro-tumorigenic activity since they participate in the degradation of extracellular matrix components and adhesion molecules,and the activation of growth factors.However,some proteases,such as ADAM metallopeptidase with thrombospondin type 1 motif 8 and kallikrein-related peptidases 5 and 10,have emerged as tumor suppressors due to their antitumoral actions in specific cancer contexts.In this article,we discuss the antitumoral effects of ADAM metallopeptidase with thrombospondin type 1 motif 8,kallikrein-related peptidases 5 and 10 that have been described to date,suggesting their potential use as novel biomarkers and therapeutic targets in cancer.
