Rheumatoid arthritis(RA),an autoimmune disease characterized by chronic inflammation of synovial tissue,is divided into two subtypes-anti-citrullinated protein antibody(ACPA)-positive and ACPA-negative RA.While the pa...Rheumatoid arthritis(RA),an autoimmune disease characterized by chronic inflammation of synovial tissue,is divided into two subtypes-anti-citrullinated protein antibody(ACPA)-positive and ACPA-negative RA.While the pathogenic mechanisms of ACPA-positive RA are well-understood,the etiology of ACPA-negative RA remains largely unknown.The association between RA and periodontitis(PD)has been observed since the early 190os,with the two diseases sharing common genetic and environmental risk factors that lead to the progressive destruction of bone and connective tissue.However,the associations between PD and the two subtypes of RA differ.This comprehensive review aims to provide an updated understanding of the epidemiological association between RA and PD,explore potential pathogenic mechanisms linking the two diseases,and highlight the key distinctions between the subtypes of RA and their respective associations with PD.We also discuss the possibility of early intervention or the treatment of the two diseases.Ultimately,this review aims to provide valuable insights for future research in this field.展开更多
RA是一种以滑膜关节的慢性炎症为主要表现的全身性自身免疫病，可以导致进行性的关节损伤和功能失衡。其病因复杂，与基因变异及环境暴露的交互作用等多种因素有关。约70％～80％的RA患者体内可产生抗瓜氨酸蛋白抗体（anti-citrullinate...RA是一种以滑膜关节的慢性炎症为主要表现的全身性自身免疫病，可以导致进行性的关节损伤和功能失衡。其病因复杂，与基因变异及环境暴露的交互作用等多种因素有关。约70％～80％的RA患者体内可产生抗瓜氨酸蛋白抗体（anti-citrullinated protein antibodies, ACPA ）,该抗体对RA的诊断具有高度特异性，并与RA患者的临床表现、治疗和预后密切相关。根据ACPA的状态，目前认为RA可分为ACPA阳性和ACPA阴性两种亚型。本文将从ACPA阴性RA的发病机制、临床特点、诊断以及治疗等方面的最新研究进展进行综述。展开更多
Background:A specific feature of the autoimmune response in rheumatoid arthritis(RA)is the presence of anti-citrullinated protein antibodies(ACPAs)in patient sera.These antibodies can appear several years before disea...Background:A specific feature of the autoimmune response in rheumatoid arthritis(RA)is the presence of anti-citrullinated protein antibodies(ACPAs)in patient sera.These antibodies can appear several years before disease onset and are involved in the development of RA.Objective:We performed proteomic analysis by mass spectroscopy to identify novel citrullinated antigens and autoantibodies in RA patients.Methods:Polypeptides isolated from the sera of RA patients were identified by Orbitrap high-precision mass spectrometry and then citrulline-containing proteins were selected.The levels of ACPAs against these newly identified citrullinated autoantigens in sera of 100 RA patients and 50 healthy controls were determined by enzyme-linked immunosorbent assays.Results:A total of 135 proteins were identified in RA patients and the protein profile included 11 citrulline-containing antigens.Three of the 11 citrullinated proteins had been reported in previous studies.ACPAs against the novel citrullinated epitopes from these proteins were increased in sera from the RA patients compared with those from healthy controls.Autoantibodies against one of the citrullinated antigens,lipopolysaccharide-binding protein(LBP),was significantly increased in RA patients and associated with disease activities.The titer of anti-citrullinated LBP antibodies(anti-cLBP)was closely related to the infection incidence in RA patients.Conclusion:Serum protein analysis by high-precision proteomic technology is a feasible method to identify novel citrullinated epitopes in RA patients.Anti-cLBP antibodies are associated with disease severity and infection in RA patients.展开更多
基金supported by the National Natural Science Foundation of China(Nos.82171768,81701600,and 82001048)the National Key Research and Development Program of China(No.2022YFC3602000)the Young Elite Scientist Support Program by CSA(Chinese Stomatological Association)(No.2020PYRC001).
文摘Rheumatoid arthritis(RA),an autoimmune disease characterized by chronic inflammation of synovial tissue,is divided into two subtypes-anti-citrullinated protein antibody(ACPA)-positive and ACPA-negative RA.While the pathogenic mechanisms of ACPA-positive RA are well-understood,the etiology of ACPA-negative RA remains largely unknown.The association between RA and periodontitis(PD)has been observed since the early 190os,with the two diseases sharing common genetic and environmental risk factors that lead to the progressive destruction of bone and connective tissue.However,the associations between PD and the two subtypes of RA differ.This comprehensive review aims to provide an updated understanding of the epidemiological association between RA and PD,explore potential pathogenic mechanisms linking the two diseases,and highlight the key distinctions between the subtypes of RA and their respective associations with PD.We also discuss the possibility of early intervention or the treatment of the two diseases.Ultimately,this review aims to provide valuable insights for future research in this field.
文摘RA是一种以滑膜关节的慢性炎症为主要表现的全身性自身免疫病，可以导致进行性的关节损伤和功能失衡。其病因复杂，与基因变异及环境暴露的交互作用等多种因素有关。约70％～80％的RA患者体内可产生抗瓜氨酸蛋白抗体（anti-citrullinated protein antibodies, ACPA ）,该抗体对RA的诊断具有高度特异性，并与RA患者的临床表现、治疗和预后密切相关。根据ACPA的状态，目前认为RA可分为ACPA阳性和ACPA阴性两种亚型。本文将从ACPA阴性RA的发病机制、临床特点、诊断以及治疗等方面的最新研究进展进行综述。
基金Peking University People's Hospital Research and Development Funds,Grant/Award Number:RDX2019-03National Natural Science Foundation of China,Grant/Award Numbers:81971520,U1903210,81671602Beijing Municipal Science and Technology Project,Grant/Award Numbers:Z191100006619112,Z191100006619110。
文摘Background:A specific feature of the autoimmune response in rheumatoid arthritis(RA)is the presence of anti-citrullinated protein antibodies(ACPAs)in patient sera.These antibodies can appear several years before disease onset and are involved in the development of RA.Objective:We performed proteomic analysis by mass spectroscopy to identify novel citrullinated antigens and autoantibodies in RA patients.Methods:Polypeptides isolated from the sera of RA patients were identified by Orbitrap high-precision mass spectrometry and then citrulline-containing proteins were selected.The levels of ACPAs against these newly identified citrullinated autoantigens in sera of 100 RA patients and 50 healthy controls were determined by enzyme-linked immunosorbent assays.Results:A total of 135 proteins were identified in RA patients and the protein profile included 11 citrulline-containing antigens.Three of the 11 citrullinated proteins had been reported in previous studies.ACPAs against the novel citrullinated epitopes from these proteins were increased in sera from the RA patients compared with those from healthy controls.Autoantibodies against one of the citrullinated antigens,lipopolysaccharide-binding protein(LBP),was significantly increased in RA patients and associated with disease activities.The titer of anti-citrullinated LBP antibodies(anti-cLBP)was closely related to the infection incidence in RA patients.Conclusion:Serum protein analysis by high-precision proteomic technology is a feasible method to identify novel citrullinated epitopes in RA patients.Anti-cLBP antibodies are associated with disease severity and infection in RA patients.
