Oncology Research Editorial Office Published:19 January 2026 The published article titled“ABCB5-ZEB1 Axis Promotes Invasion and Metastasis in Breast Cancer Cells”has been retracted from Oncology Research,Vol.25,No.3...Oncology Research Editorial Office Published:19 January 2026 The published article titled“ABCB5-ZEB1 Axis Promotes Invasion and Metastasis in Breast Cancer Cells”has been retracted from Oncology Research,Vol.25,No.3,2017,pp.305-316.DOI:10.3727/096504016X14734149559061 URL:https://www.techscience.com/or/v25n3/56810.展开更多
Objectives:Acquired resistance to paclitaxel represents a critical barrier to the effective chemotherapy of non-small cell lung cancer(NSCLC).The present study aimed to elucidate the molecular and pharmacological mech...Objectives:Acquired resistance to paclitaxel represents a critical barrier to the effective chemotherapy of non-small cell lung cancer(NSCLC).The present study aimed to elucidate the molecular and pharmacological mechanisms promoting paclitaxel resistance in NSCLC and to explore potential strategies for overcoming this resistance.Methods:Here,we report an integrated pharmacological and analytical approach to quantify paclitaxel disposition and overcome resistance in a A549/TAX cell model(paclitaxel-resistant A549 cells).Results:Cell counting kit-8(CCK-8)assay,colony formation,and apoptosis assays confirmed that A549/TAX cells exhibited marked resistance to paclitaxel relative to parental A549 cells.Based on transcriptome profiling by RNA sequencing analysis and validation by western blotting assay,we found that the expression of the ATP-binding cassette subfamily B member 1(ABCB1)(the encoded protein is termed P-glycoprotein)was significantly upregulated in resistant cells.By using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),we demonstrated that ABCB1 overexpression promotes enhanced efflux of intracellular paclitaxel,thereby lowering its cytotoxic accumulation.Genetic silencing of ABCB1 or pharmacological inhibition with the specific P-glycoprotein modulator elacridar or tariquidar restored intracellular paclitaxel levels,as determined by UPLC-MS/MS,and synergistically decreased cell viability as observed in CCK-8 assay.Conclusion:These findings reveal that the ABCB1-mediated drug efflux is a crucial mechanism underlying paclitaxel resistance in NSCLC cells,with UPLC-MS/MS serving as a sensitive analytical method to detect paclitaxel concentration.Inhibition of ABCB1 is a promising therapeutic strategy to resensitize resistant tumor cells to paclitaxel.展开更多
Objective:To summarize the clinical manifestations,gene mutation type,treatment,and follow-up results of one admitted infant with biliary cholestatic liver disease caused by ABCB4 gene mutation,so as to improve the un...Objective:To summarize the clinical manifestations,gene mutation type,treatment,and follow-up results of one admitted infant with biliary cholestatic liver disease caused by ABCB4 gene mutation,so as to improve the understanding of this rare disease.Methods:A retrospective analysis was conducted on the clinical manifestations,laboratory examinations,gene mutation type,treatment,and follow-up data of one infant with biliary cholestatic liver disease caused by ABCB4 gene mutation.Results:The patient was a 1 month 23-day old male infant.His main clinical manifestations included dark yellow skin,rash,and pruritus.The disease onset was early,and his serum gamma-glutamyl transpeptidase level was elevated.Genetic analysis revealed two newly identified point mutations in the ABCB4 gene,namely c.1576G>A and c.2596A>G heterozygotes,which were inherited from his father.The infant was cured after treatment with integrated traditional Chinese and Western medicine,and no recurrence was observed during a 6-month follow-up.Conclusion:This study reports a case of biliary cholestatic liver disease caused by ABCB4 gene mutation in an infant,which expands the mutation spectrum of the ABCB4 gene.It also provides a reference for the early diagnosis and treatment of this rare disease using integrated traditional Chinese and Western medicine.展开更多
文摘Oncology Research Editorial Office Published:19 January 2026 The published article titled“ABCB5-ZEB1 Axis Promotes Invasion and Metastasis in Breast Cancer Cells”has been retracted from Oncology Research,Vol.25,No.3,2017,pp.305-316.DOI:10.3727/096504016X14734149559061 URL:https://www.techscience.com/or/v25n3/56810.
基金supported by grants from the National Natural Science Foundation of China(Grant No.82172840)Gusu Health Talents Project of Suzhou Municipal Health Commission(Grant Nos.GSWS2023007 and GSWS2022062)+2 种基金Suzhou Science and Technology Development Plan Project(Grant No.SYW2024005)Chinese Pharmaceutical Association Hospital Pharmacy Department(Grant No.CPA-Z05-ZC-2024002)Jiangsu Research Hospital Association for Precision Medication(Grant No.JY202202).
文摘Objectives:Acquired resistance to paclitaxel represents a critical barrier to the effective chemotherapy of non-small cell lung cancer(NSCLC).The present study aimed to elucidate the molecular and pharmacological mechanisms promoting paclitaxel resistance in NSCLC and to explore potential strategies for overcoming this resistance.Methods:Here,we report an integrated pharmacological and analytical approach to quantify paclitaxel disposition and overcome resistance in a A549/TAX cell model(paclitaxel-resistant A549 cells).Results:Cell counting kit-8(CCK-8)assay,colony formation,and apoptosis assays confirmed that A549/TAX cells exhibited marked resistance to paclitaxel relative to parental A549 cells.Based on transcriptome profiling by RNA sequencing analysis and validation by western blotting assay,we found that the expression of the ATP-binding cassette subfamily B member 1(ABCB1)(the encoded protein is termed P-glycoprotein)was significantly upregulated in resistant cells.By using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),we demonstrated that ABCB1 overexpression promotes enhanced efflux of intracellular paclitaxel,thereby lowering its cytotoxic accumulation.Genetic silencing of ABCB1 or pharmacological inhibition with the specific P-glycoprotein modulator elacridar or tariquidar restored intracellular paclitaxel levels,as determined by UPLC-MS/MS,and synergistically decreased cell viability as observed in CCK-8 assay.Conclusion:These findings reveal that the ABCB1-mediated drug efflux is a crucial mechanism underlying paclitaxel resistance in NSCLC cells,with UPLC-MS/MS serving as a sensitive analytical method to detect paclitaxel concentration.Inhibition of ABCB1 is a promising therapeutic strategy to resensitize resistant tumor cells to paclitaxel.
基金Key Project of School-Level Fund of Dongguan Polytechnic:“Exploring the Role and Molecular Mechanism of Astragalus membranaceus in Preventing and Treating Bronchopulmonary Dysplasia through Its‘Qi-Invigorating’Function Based on the HMGB1/RAGE/TLR4 Signaling Pathway”(Project No.:2024a07)。
文摘Objective:To summarize the clinical manifestations,gene mutation type,treatment,and follow-up results of one admitted infant with biliary cholestatic liver disease caused by ABCB4 gene mutation,so as to improve the understanding of this rare disease.Methods:A retrospective analysis was conducted on the clinical manifestations,laboratory examinations,gene mutation type,treatment,and follow-up data of one infant with biliary cholestatic liver disease caused by ABCB4 gene mutation.Results:The patient was a 1 month 23-day old male infant.His main clinical manifestations included dark yellow skin,rash,and pruritus.The disease onset was early,and his serum gamma-glutamyl transpeptidase level was elevated.Genetic analysis revealed two newly identified point mutations in the ABCB4 gene,namely c.1576G>A and c.2596A>G heterozygotes,which were inherited from his father.The infant was cured after treatment with integrated traditional Chinese and Western medicine,and no recurrence was observed during a 6-month follow-up.Conclusion:This study reports a case of biliary cholestatic liver disease caused by ABCB4 gene mutation in an infant,which expands the mutation spectrum of the ABCB4 gene.It also provides a reference for the early diagnosis and treatment of this rare disease using integrated traditional Chinese and Western medicine.
