To solve the problem of circulating power of dual active bridge(DAB)DC-DC converter over a wide voltage conversion ratio,this paper proposes a novel synchronous PWM(S-PWM)modulation.Existence of circulating power incr...To solve the problem of circulating power of dual active bridge(DAB)DC-DC converter over a wide voltage conversion ratio,this paper proposes a novel synchronous PWM(S-PWM)modulation.Existence of circulating power increases current stress of devices and decreases efficiency,especially under light load conditions.Several modulation methods have been proposed to overcome the problem.They can reduce or eliminate either input or output side circulating power.In contrast,S-PWM not only eliminates both sides circulating power and reduces current stress,but also achieves zero-current-switching(ZCS)turn-on for all switches and ZCS turn-off for most across the full power range.No auxiliary or snubber circuits are increased.In addition,the control can be simplified so the transmitted power is related to only one variable.The S-PWM has four cases under different gain and power conditions.The detailed operation principle and modes of DAB under S-PWM are analyzed in the paper.In addition,four modulations in literature are discussed,and corresponding comparative analyses with S-PWM are given.Finally,a laboratory prototype is built to verify advantages and effectiveness of the proposed modulation.展开更多
Grid-Forming(GFM)converters are prone to fault-induced overcurrent and power angle instability during grid fault-induced voltage sags.To address this,this paper develops a multi-loop coordinated fault ridethrough(FRT)...Grid-Forming(GFM)converters are prone to fault-induced overcurrent and power angle instability during grid fault-induced voltage sags.To address this,this paper develops a multi-loop coordinated fault ridethrough(FRT)control strategy based on a power outer loop and voltage-current inner loops,aiming to enhance the stability and current-limiting capability of GFM converters during grid fault conditions.During voltage sags,the GFM converter’s voltage source behavior is maintained by dynamically adjusting the reactive power reference to provide voltage support,thereby effectively suppressing the steady-state component of the fault current.To address the active power imbalance induced by voltage sags,a dynamic active power reference correction method based on apparent power is designed to mitigate power angle oscillations and limit transient current.Moreover,an adaptive virtual impedance loop is implemented to enhance dynamic transient current-limiting performance during the fault initiation phase.This approach improves the responsiveness of the inner loop and ensures safe system operation under various fault severities.Under asymmetric fault conditions,a negative-sequence reactive current compensation strategy is incorporated to further suppress negative-sequence voltage and improve voltage symmetry.The proposed control scheme enables coordinated operation of multiple control objectives,including voltage support,current suppression,and power angle stability,across different fault scenarios.Finally,MATLAB/Simulink simulation results validate the effectiveness of the proposed strategy,showcasing its superior performance in current limiting and power angle stability,thereby significantly enhancing the system’s fault ride-through capability.展开更多
The transient synchronization stability of grid-forming converters(GFMCs)is significantly challenged under grid voltage sags.Continuous efforts have been devoted to analyzing the GFMC transient stability,with limited ...The transient synchronization stability of grid-forming converters(GFMCs)is significantly challenged under grid voltage sags.Continuous efforts have been devoted to analyzing the GFMC transient stability,with limited attention paid to the impacts of control loop dynamics.However,the complex control dynamics,especially the interactions between the active/reactive power control loops and the current saturation process(CSP),are crucial for accurately describing the transient behavior and evaluating the stability.Thus,in this study,a new large-signal GFMC model is established,considering the reactive power control(RPC)with different kinds of controllers and the CSP simultaneously.It is revealed that GFMC does not switch to the current-limited mode immediately,and the dynamics of RPC further affect the transient behavior before the current limiting significantly.Hence,the complex control dynamics can alter the mode switching point of current saturation,thereby increasing the risk of loss of synchronization(LOS).Based on the above findings,comprehensive comparisons of typical RPC controllers are presented to facilitate practical engineering applications.A unified stability enhancement method is proposed for solving the problem of LOS.Finally,experiments validate the correctness of the analysis and the effectiveness of the proposed control strategy.展开更多
In the future power-electronics-dominated power systems,grid-forming(GFM)converters have been regarded as important devices to actively establish frequency and voltage,so as to provide essential grid support.However,d...In the future power-electronics-dominated power systems,grid-forming(GFM)converters have been regarded as important devices to actively establish frequency and voltage,so as to provide essential grid support.However,due to their voltage source behavior and emulated swing dynamics,GFM converters may encounter low-frequency oscillations(LFOs)when connected to strong grids,which belongs to the self-stability problem of GFM converters.Moreover,GFM converters will also interact with grid-following(GFL)converters and thus impact the mid-frequency oscillations(MFOs)induced by phase-locked loops(PLLs).It has been preliminarily shown in the literature that GFM converters can help stabilize the PLL-induced MFOs,but currently,there is a lack of systematic design methods to coordinate the self-stability and stabilizing ability of GFM converters.This paper addresses this gap by revisiting the impedance model of a typical GFM converter and briefly analyze the oscillations caused by converters.Based on our analysis,we propose a frequency-partitioned synthesis design framework to enable dynamic virtual impedance(DVI)in GFM converters,aiming to enhance their self-stability and stabilizing ability simultaneously.Particularly,a self-stabilizing module is designed to ensure robust device-level damping,with control parameters auto-tuned using H∞methods.In parallel,a stabilizing module is introduced to stabilize GFL converters and enhance the system-level stability,which utilizes a perceive-and-optimize tuning strategy.Simulation results validate the effectiveness of the proposed synthesis DVI framework.展开更多
A multi-phase stacked interleaved buck converter(SIBC)is suitable for large-power water electrolysis applications due to its merits of high current output capability and zero output current ripple.However,the auxiliar...A multi-phase stacked interleaved buck converter(SIBC)is suitable for large-power water electrolysis applications due to its merits of high current output capability and zero output current ripple.However,the auxiliary converter used to compensate for the current ripple still has to withstand high voltage stress.This paper proposes a new multi-phase SIBC applied in the multicarrier energy system integrating electricity,heat,and hydrogen.A resistor-capacitor voltage divider is used to provide the input voltage of the auxiliary converter and as a heater for the thermal loads.Thus,the voltage stress of the auxiliary converter can be reduced at a low cost,and the size of the filter inductor can be reduced.With accurate voltage and current analysis and appropriate parameter design,the voltage stresses of both the switches and capacitors in the auxiliary converter can be further limited within an expected range.The experimental results verify the correctness of the topology,modulation,analysis,and design methods.A comparison with the conventional method is made in terms of cost,volume,and efficiency to show the advantages of the proposed method.展开更多
As key equipment in medium voltage DC(MVDC)systems,modular multilevel AC/DC and DC/DC converters(MM-AC/DC,MM-DC/DC)have drawn marvelous attractions.However,research on DC fault ride-through focuses on MM-AC/DC,and the...As key equipment in medium voltage DC(MVDC)systems,modular multilevel AC/DC and DC/DC converters(MM-AC/DC,MM-DC/DC)have drawn marvelous attractions.However,research on DC fault ride-through focuses on MM-AC/DC,and the fault current elimination for MM-DC/DC remains a research gap,which limits the wide application of the MVDC system.To fulfil this research gap,the contribution of this paper is revealing the fault current characteristics of MM-DC/DC based on half-bridge and full-bridge submodules(HBSM and FBSM)and proposing a novel MM-DC/DC based on hybrid HBSM and thyristor-diode module(TDM).By integrating TDM in the upper bridge arm of one phase and the down bridge arm of the other phase in MM-DC/DC,the MM-DC/DC achieves self-elimination of fault currents.The basic concept is using the energy at the healthy side to modulate a reverse voltage source(RVS)at the faulty side of MM-DC/DC,forcing fault current through TDM pass across zero.TDM can extinguish the resulting fault current.The parameter design and control strategy of the novel MM-DC/DC are discussed.Simulation is carried out for verification,and the results show that fault current can be eliminated within several milliseconds without causing excessive operating losses and costs.展开更多
In order to solve the problems of slow dynamic response and difficult multi-source coordination of solar electric vehicle charging stations under intermittent renewable energy,this paper proposes a hardware-algorithm ...In order to solve the problems of slow dynamic response and difficult multi-source coordination of solar electric vehicle charging stations under intermittent renewable energy,this paper proposes a hardware-algorithm co-design framework:the T-type three-level bidirectional converter(100 kHz switching frequency)based on silicon carbide(SiC)MOSFET is deeply integrated with fuzzy model predictive control(Fuzzy-MPC).At the hardware level,the switching trajectory and resonance suppression circuit(attenuation resonance peak 18 dB)are optimized,and the total loss is reduced by 23%compared with the traditional silicon-based IGBT.At the algorithm level,the adaptive parameter update mechanism and multi-objective rolling optimization are adopted,and the 5 ms level dynamic power allocation is realized by relying on edge computing.Experiments on 800 V DC microgrid(including 600 kW photovoltaic and 150 A·h energy storage)built based on MATLAB/Simulink hardware-in-the-loop(HIL)platform show that the system shortens the battery charging time from 42 to 28 min(the charging speed is increased by 33%).Through the 78%valley power utilization rate,the power purchase cost of high-priced power grids was significantly reduced,and the levelized electricity price decreased by 10.3%;Under the irradiation fluctuation,the renewable energy consumption rate increases by 10.1%,and the DC bus voltage fluctuation is stable within±10 V when the load step is±30%.The co-design provides an economically feasible and dynamically robust solution for the efficient integration of PV-ESG-EV in the smart grid.展开更多
To address the issue of abnormal energy consumption fluctuations in the converter steelmaking process,an integrated diagnostic method combining the gray wolf optimization(GWO)algorithm,support vector machine(SVM),and ...To address the issue of abnormal energy consumption fluctuations in the converter steelmaking process,an integrated diagnostic method combining the gray wolf optimization(GWO)algorithm,support vector machine(SVM),and K-means clustering was proposed.Eight input parameters—derived from molten iron conditions and external factors—were selected as feature variables.A GWO-SVM model was developed to accurately predict the energy consumption of individual heats.Based on the prediction results,the mean absolute percentage error and maximum relative error of the test set were employed as criteria to identify heats with abnormal energy usage.For these heats,the K-means clustering algorithm was used to determine benchmark values of influencing factors from similar steel grades,enabling root-cause diagnosis of excessive energy consumption.The proposed method was applied to real production data from a converter in a steel plant.The analysis reveals that heat sample No.44 exhibits abnormal energy consumption,due to gas recovery being 1430.28 kg of standard coal below the benchmark level.A secondary contributing factor is a steam recovery shortfall of 237.99 kg of standard coal.This integrated approach offers a scientifically grounded tool for energy management in converter operations and provides valuable guidance for optimizing process parameters and enhancing energy efficiency.展开更多
This review paper examines the various types of electrical generators used to convert wave energy into electrical energy.The focus is on both linear and rotary generators,including their design principles,operational ...This review paper examines the various types of electrical generators used to convert wave energy into electrical energy.The focus is on both linear and rotary generators,including their design principles,operational efficiencies,and technological advancements.Linear generators,such as Induction,permanent magnet synchronous,and switched reluctance types,are highlighted for their direct conversion capability,eliminating the need for mechanical gearboxes.Rotary Induction generators,permanent magnet synchronous generators,and doubly-fed Induction generators are evaluated for their established engineering principles and integration with existing grid infrastructure.The paper discusses the historical development,environmental benefits,and ongoing advancements in wave energy technologies,emphasizing the increasing feasibility and scalability of wave energy as a renewable source.Through a comprehensive analysis,this review provides insights into the current state and future prospects of electrical generators in wave energy conversion,underscoring their potential to significantly reduce reliance on fossil fuels and mitigate environmental impacts.展开更多
目的研究昆明地区RhD初筛阴性献血者RHD基因的多态性及其分子机制,为建立区域性献血者RHD基因数据库提供数据支持。方法选择昆明地区2023年11月-2024年8月初筛RhD阴性标本218例,采用间接抗球蛋白试验法(IAT)进行RhD阴性确认,采用盐水试...目的研究昆明地区RhD初筛阴性献血者RHD基因的多态性及其分子机制,为建立区域性献血者RHD基因数据库提供数据支持。方法选择昆明地区2023年11月-2024年8月初筛RhD阴性标本218例,采用间接抗球蛋白试验法(IAT)进行RhD阴性确认,采用盐水试管法进行RhCE表型鉴定。提取全血基因组DNA,采用PCR-SSP法/SSP荧光PCR染料法进行RHD基因分型,对无法确定基因型的标本进行RHD基因1~10外显子Sanger测序分析。结果检出RhD真阴性表型179例(82.11%),其中RHD*01N.01(RHD全缺失)型154例(86.03%),表型以ccee为主(87.01%);携带非功能性RHD等位基因25例(13.97%),包括RHD*01N.0320例、RHD*01N.163例、RHD*01N.051例、RHD*01N.591例,表型以Ccee为主(64%)。检出D变异型39例(15.89%),其中RHD*DEL1(c.1227G>A)型34例,表型均为C抗原阳性(Ccee 27例,CCee 7例);弱D/部分D型4例,包括RHD*DVI.32例、RHD*weak D type 711例、RHD*weak D type1081例;另检出1例RHD*01/RHD*01N.01,基因型与血清学表型结果不一致。RHD*01N.01女性献血者不规则抗体(主要为抗-D)阳性率9.84%。结论昆明地区RhD初筛阴性献血者RHD基因多态性显著,RhD真阴性比例高于国内部分地区,D变异型以“亚洲型”DEL为主,比例低于国内部分地区。研究结果为本地区RhD阴性和D变异型个体精准输血提供了理论和数据支持。展开更多
文摘To solve the problem of circulating power of dual active bridge(DAB)DC-DC converter over a wide voltage conversion ratio,this paper proposes a novel synchronous PWM(S-PWM)modulation.Existence of circulating power increases current stress of devices and decreases efficiency,especially under light load conditions.Several modulation methods have been proposed to overcome the problem.They can reduce or eliminate either input or output side circulating power.In contrast,S-PWM not only eliminates both sides circulating power and reduces current stress,but also achieves zero-current-switching(ZCS)turn-on for all switches and ZCS turn-off for most across the full power range.No auxiliary or snubber circuits are increased.In addition,the control can be simplified so the transmitted power is related to only one variable.The S-PWM has four cases under different gain and power conditions.The detailed operation principle and modes of DAB under S-PWM are analyzed in the paper.In addition,four modulations in literature are discussed,and corresponding comparative analyses with S-PWM are given.Finally,a laboratory prototype is built to verify advantages and effectiveness of the proposed modulation.
文摘Grid-Forming(GFM)converters are prone to fault-induced overcurrent and power angle instability during grid fault-induced voltage sags.To address this,this paper develops a multi-loop coordinated fault ridethrough(FRT)control strategy based on a power outer loop and voltage-current inner loops,aiming to enhance the stability and current-limiting capability of GFM converters during grid fault conditions.During voltage sags,the GFM converter’s voltage source behavior is maintained by dynamically adjusting the reactive power reference to provide voltage support,thereby effectively suppressing the steady-state component of the fault current.To address the active power imbalance induced by voltage sags,a dynamic active power reference correction method based on apparent power is designed to mitigate power angle oscillations and limit transient current.Moreover,an adaptive virtual impedance loop is implemented to enhance dynamic transient current-limiting performance during the fault initiation phase.This approach improves the responsiveness of the inner loop and ensures safe system operation under various fault severities.Under asymmetric fault conditions,a negative-sequence reactive current compensation strategy is incorporated to further suppress negative-sequence voltage and improve voltage symmetry.The proposed control scheme enables coordinated operation of multiple control objectives,including voltage support,current suppression,and power angle stability,across different fault scenarios.Finally,MATLAB/Simulink simulation results validate the effectiveness of the proposed strategy,showcasing its superior performance in current limiting and power angle stability,thereby significantly enhancing the system’s fault ride-through capability.
基金supported by the National Natural Science Foundation of China under Grant 52277184 and Grant 52277183.
文摘The transient synchronization stability of grid-forming converters(GFMCs)is significantly challenged under grid voltage sags.Continuous efforts have been devoted to analyzing the GFMC transient stability,with limited attention paid to the impacts of control loop dynamics.However,the complex control dynamics,especially the interactions between the active/reactive power control loops and the current saturation process(CSP),are crucial for accurately describing the transient behavior and evaluating the stability.Thus,in this study,a new large-signal GFMC model is established,considering the reactive power control(RPC)with different kinds of controllers and the CSP simultaneously.It is revealed that GFMC does not switch to the current-limited mode immediately,and the dynamics of RPC further affect the transient behavior before the current limiting significantly.Hence,the complex control dynamics can alter the mode switching point of current saturation,thereby increasing the risk of loss of synchronization(LOS).Based on the above findings,comprehensive comparisons of typical RPC controllers are presented to facilitate practical engineering applications.A unified stability enhancement method is proposed for solving the problem of LOS.Finally,experiments validate the correctness of the analysis and the effectiveness of the proposed control strategy.
基金supported by National Natural Science Foundation of China(U24B6008,U22B6008)State Grid Zhejiang Electric Power Co.,Ltd.Science,and Technology Project(B311DS240015).
文摘In the future power-electronics-dominated power systems,grid-forming(GFM)converters have been regarded as important devices to actively establish frequency and voltage,so as to provide essential grid support.However,due to their voltage source behavior and emulated swing dynamics,GFM converters may encounter low-frequency oscillations(LFOs)when connected to strong grids,which belongs to the self-stability problem of GFM converters.Moreover,GFM converters will also interact with grid-following(GFL)converters and thus impact the mid-frequency oscillations(MFOs)induced by phase-locked loops(PLLs).It has been preliminarily shown in the literature that GFM converters can help stabilize the PLL-induced MFOs,but currently,there is a lack of systematic design methods to coordinate the self-stability and stabilizing ability of GFM converters.This paper addresses this gap by revisiting the impedance model of a typical GFM converter and briefly analyze the oscillations caused by converters.Based on our analysis,we propose a frequency-partitioned synthesis design framework to enable dynamic virtual impedance(DVI)in GFM converters,aiming to enhance their self-stability and stabilizing ability simultaneously.Particularly,a self-stabilizing module is designed to ensure robust device-level damping,with control parameters auto-tuned using H∞methods.In parallel,a stabilizing module is introduced to stabilize GFL converters and enhance the system-level stability,which utilizes a perceive-and-optimize tuning strategy.Simulation results validate the effectiveness of the proposed synthesis DVI framework.
基金supported in part by the National Natural Science Foundation of China(52077190)Cultivation Project for Basic Research and Innovation of Yanshan University(2021LGQN007)Science and Technology Project of Hebei Education Department(QN2024202).
文摘A multi-phase stacked interleaved buck converter(SIBC)is suitable for large-power water electrolysis applications due to its merits of high current output capability and zero output current ripple.However,the auxiliary converter used to compensate for the current ripple still has to withstand high voltage stress.This paper proposes a new multi-phase SIBC applied in the multicarrier energy system integrating electricity,heat,and hydrogen.A resistor-capacitor voltage divider is used to provide the input voltage of the auxiliary converter and as a heater for the thermal loads.Thus,the voltage stress of the auxiliary converter can be reduced at a low cost,and the size of the filter inductor can be reduced.With accurate voltage and current analysis and appropriate parameter design,the voltage stresses of both the switches and capacitors in the auxiliary converter can be further limited within an expected range.The experimental results verify the correctness of the topology,modulation,analysis,and design methods.A comparison with the conventional method is made in terms of cost,volume,and efficiency to show the advantages of the proposed method.
基金supported by Science and Technology Project of SGCC(5108-202218280A-2-370-XG).
文摘As key equipment in medium voltage DC(MVDC)systems,modular multilevel AC/DC and DC/DC converters(MM-AC/DC,MM-DC/DC)have drawn marvelous attractions.However,research on DC fault ride-through focuses on MM-AC/DC,and the fault current elimination for MM-DC/DC remains a research gap,which limits the wide application of the MVDC system.To fulfil this research gap,the contribution of this paper is revealing the fault current characteristics of MM-DC/DC based on half-bridge and full-bridge submodules(HBSM and FBSM)and proposing a novel MM-DC/DC based on hybrid HBSM and thyristor-diode module(TDM).By integrating TDM in the upper bridge arm of one phase and the down bridge arm of the other phase in MM-DC/DC,the MM-DC/DC achieves self-elimination of fault currents.The basic concept is using the energy at the healthy side to modulate a reverse voltage source(RVS)at the faulty side of MM-DC/DC,forcing fault current through TDM pass across zero.TDM can extinguish the resulting fault current.The parameter design and control strategy of the novel MM-DC/DC are discussed.Simulation is carried out for verification,and the results show that fault current can be eliminated within several milliseconds without causing excessive operating losses and costs.
基金Jiangsu Provincial College Student Innovation and Entrepreneurship Program(Grant No.SJCX25_2184)—“Multi-energy Complementary Optimization and Vehicle-Storage Bidirectional Interaction Technology Driven by Novel 5E Framework”(Principal Investigator:Yuan-Yuan ShiFunding Agency:Jiangsu Provincial Education Department)+3 种基金Huaian Natural Science Research Project(Grant No.HAB2024046)—“Optimal Control of Flexible Cold-Heat-Power Integrated System with Source-Grid-Load-Storage Coordination”(Principal Investigator:Jie JiFunding Agency:Huaian Science and Technology Bureau)Huaiyin Institute of TechnologyUniversity-funded Project(GrantNo.HGYK202511)—“Data-driven CooperativeOptimization Dispatch for Source-Grid-Load Systems”(Principal Investigator:Chu-Tong ZhangFunding Agency:Huaiyin Institute of Technology).
文摘In order to solve the problems of slow dynamic response and difficult multi-source coordination of solar electric vehicle charging stations under intermittent renewable energy,this paper proposes a hardware-algorithm co-design framework:the T-type three-level bidirectional converter(100 kHz switching frequency)based on silicon carbide(SiC)MOSFET is deeply integrated with fuzzy model predictive control(Fuzzy-MPC).At the hardware level,the switching trajectory and resonance suppression circuit(attenuation resonance peak 18 dB)are optimized,and the total loss is reduced by 23%compared with the traditional silicon-based IGBT.At the algorithm level,the adaptive parameter update mechanism and multi-objective rolling optimization are adopted,and the 5 ms level dynamic power allocation is realized by relying on edge computing.Experiments on 800 V DC microgrid(including 600 kW photovoltaic and 150 A·h energy storage)built based on MATLAB/Simulink hardware-in-the-loop(HIL)platform show that the system shortens the battery charging time from 42 to 28 min(the charging speed is increased by 33%).Through the 78%valley power utilization rate,the power purchase cost of high-priced power grids was significantly reduced,and the levelized electricity price decreased by 10.3%;Under the irradiation fluctuation,the renewable energy consumption rate increases by 10.1%,and the DC bus voltage fluctuation is stable within±10 V when the load step is±30%.The co-design provides an economically feasible and dynamically robust solution for the efficient integration of PV-ESG-EV in the smart grid.
基金support from the National Key R&D Program of China(Grant No.2020YFB1711100).
文摘To address the issue of abnormal energy consumption fluctuations in the converter steelmaking process,an integrated diagnostic method combining the gray wolf optimization(GWO)algorithm,support vector machine(SVM),and K-means clustering was proposed.Eight input parameters—derived from molten iron conditions and external factors—were selected as feature variables.A GWO-SVM model was developed to accurately predict the energy consumption of individual heats.Based on the prediction results,the mean absolute percentage error and maximum relative error of the test set were employed as criteria to identify heats with abnormal energy usage.For these heats,the K-means clustering algorithm was used to determine benchmark values of influencing factors from similar steel grades,enabling root-cause diagnosis of excessive energy consumption.The proposed method was applied to real production data from a converter in a steel plant.The analysis reveals that heat sample No.44 exhibits abnormal energy consumption,due to gas recovery being 1430.28 kg of standard coal below the benchmark level.A secondary contributing factor is a steam recovery shortfall of 237.99 kg of standard coal.This integrated approach offers a scientifically grounded tool for energy management in converter operations and provides valuable guidance for optimizing process parameters and enhancing energy efficiency.
文摘This review paper examines the various types of electrical generators used to convert wave energy into electrical energy.The focus is on both linear and rotary generators,including their design principles,operational efficiencies,and technological advancements.Linear generators,such as Induction,permanent magnet synchronous,and switched reluctance types,are highlighted for their direct conversion capability,eliminating the need for mechanical gearboxes.Rotary Induction generators,permanent magnet synchronous generators,and doubly-fed Induction generators are evaluated for their established engineering principles and integration with existing grid infrastructure.The paper discusses the historical development,environmental benefits,and ongoing advancements in wave energy technologies,emphasizing the increasing feasibility and scalability of wave energy as a renewable source.Through a comprehensive analysis,this review provides insights into the current state and future prospects of electrical generators in wave energy conversion,underscoring their potential to significantly reduce reliance on fossil fuels and mitigate environmental impacts.
文摘目的研究昆明地区RhD初筛阴性献血者RHD基因的多态性及其分子机制,为建立区域性献血者RHD基因数据库提供数据支持。方法选择昆明地区2023年11月-2024年8月初筛RhD阴性标本218例,采用间接抗球蛋白试验法(IAT)进行RhD阴性确认,采用盐水试管法进行RhCE表型鉴定。提取全血基因组DNA,采用PCR-SSP法/SSP荧光PCR染料法进行RHD基因分型,对无法确定基因型的标本进行RHD基因1~10外显子Sanger测序分析。结果检出RhD真阴性表型179例(82.11%),其中RHD*01N.01(RHD全缺失)型154例(86.03%),表型以ccee为主(87.01%);携带非功能性RHD等位基因25例(13.97%),包括RHD*01N.0320例、RHD*01N.163例、RHD*01N.051例、RHD*01N.591例,表型以Ccee为主(64%)。检出D变异型39例(15.89%),其中RHD*DEL1(c.1227G>A)型34例,表型均为C抗原阳性(Ccee 27例,CCee 7例);弱D/部分D型4例,包括RHD*DVI.32例、RHD*weak D type 711例、RHD*weak D type1081例;另检出1例RHD*01/RHD*01N.01,基因型与血清学表型结果不一致。RHD*01N.01女性献血者不规则抗体(主要为抗-D)阳性率9.84%。结论昆明地区RhD初筛阴性献血者RHD基因多态性显著,RhD真阴性比例高于国内部分地区,D变异型以“亚洲型”DEL为主,比例低于国内部分地区。研究结果为本地区RhD阴性和D变异型个体精准输血提供了理论和数据支持。
