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3D Model Reconstruction of Aluminum Foam Cross-Sectional Sequence Images Based on Milling
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作者 Xu Feng Zhiguo Dong +1 位作者 Bo Li Hui Peng 《Journal of Beijing Institute of Technology》 2025年第5期458-481,共24页
This study introduces a novel method for reconstructing the 3D model of aluminum foam using cross-sectional sequence images.Combining precision milling and image acquisition,high-qual-ity cross-sectional images are ob... This study introduces a novel method for reconstructing the 3D model of aluminum foam using cross-sectional sequence images.Combining precision milling and image acquisition,high-qual-ity cross-sectional images are obtained.Pore structures are segmented by the U-shaped network(U-Net)neural network integrated with the Canny edge detection operator,ensuring accurate pore delineation and edge extraction.The trained U-Net achieves 98.55%accuracy.The 2D data are superimposed and processed into 3D point clouds,enabling reconstruction of the pore structure and aluminum skeleton.Analysis of pore 01 shows the cross-sectional area initially increases,and then decreases with milling depth,with a uniform point distribution of 40 per layer.The reconstructed model exhibits a porosity of 77.5%,with section overlap rates between the 2D pore segmentation and the reconstructed model exceeding 96%,confirming high fidelity.Equivalent sphere diameters decrease with size,averaging 1.95 mm.Compression simulations reveal that the stress-strain curve of the 3D reconstruction model of aluminum foam exhibits fluctuations,and the stresses in the reconstruction model concentrate on thin cell walls,leading to localized deformations.This method accurately restores the aluminum foam’s complex internal structure,improving reconstruction preci-sion and simulation reliability.The approach offers a cost-efficient,high-precision technique for optimizing material performance in engineering applications. 展开更多
关键词 aluminum foam section milling cross-sectional sequence images U-Net neural network 3d model reconstruction compression simulation
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Subtle traps prediction using sequence stratigraphy and 3D seismic technology: A case study from Qikou depression in Huanghua basin 被引量:1
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作者 MAO Ning-bo DAI Ta-gen PENG Sheng-lin 《Journal of Central South University of Technology》 2005年第z1期141-145,共5页
Forecasting subtle traps by sequence stratigraphy and 3D seismic data is a sensitive topic in hydrocarbon exploration. Research on subtle traps by geophysical data is the most popular and difficult. Based on the suffi... Forecasting subtle traps by sequence stratigraphy and 3D seismic data is a sensitive topic in hydrocarbon exploration. Research on subtle traps by geophysical data is the most popular and difficult. Based on the sufficiently drilling data, log data, core data and 3D seismic data, sediment sequence of Qikou depression, Huanghua basin was partitioned by using sequence stratigraphy theory. Each sediment sequence system mode was built. Sediment faces of subtle traps were pointed out. Dominating factors forming subtle traps were analyzed. Sandstone seismic rock physics and its response were studied in Tertiary System. Sandstone geophysical response and elastic modulus vary laws with pressure, temperature, porosity, depth were built. Experimental result and practice shows that it is possible using seismic information forecasting subtle traps. Integrated using geology, log, drilling data, special seismic processing technique, interpretation technique, high precision horizon calibration technique, 3D seismic visualizing interpretation, seismic coherence analysis, attribute analysis, logging-constrained inversion, time frequency analysis, subtle trapsobject is identified and interpreted. Finally, advantage object of subtle trap in this area was determined. Bottomland sand stratigraphic and lithologic reservoirs in Qinan slope zone have been founded by means of high resolution 3D seismic data field technique, high resolution 3D seismic data processing technique and seismic wave impendence inversion technique. 展开更多
关键词 Huanghua BASIN sequence STRATIGRAPHY subtle TRAP SEISMIC exploration 3d SEISMIC ultrasonic wave
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Rapid Texture Mapping from Image Sequences for Building Geometry Model 被引量:1
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作者 ZHANG Zuxun WU Jun ZHANG Jianqing 《Geo-Spatial Information Science》 2003年第3期8-15,31,共9页
An effective approach,mapping the texture for building model based on the digital photogrammetric theory,is proposed.The easily-acquired image sequences from digital video camera on helicopter are used as texture reso... An effective approach,mapping the texture for building model based on the digital photogrammetric theory,is proposed.The easily-acquired image sequences from digital video camera on helicopter are used as texture resource,and the correspondence between the space edge in building geometry model and its line feature in image sequences is determined semi-automatically.The experimental results in production of three-dimensional data for car navigation show us an attractive future both in efficiency and effect. 展开更多
关键词 image sequences video camera on helicopter 3d reconstruction texture mapping digital photogrammetry
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Statistical properties of nucleotide clusters in DNA sequences
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作者 成军 章林溪 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE EI CAS CSCD 2005年第5期408-412,共5页
Using the complete genome of Plasmodium falciparum 3D7 which has 14 chromosomes as an example, we have examined the distribution functions for the amount of C or G and A or T consecutively and non-overlapping blocks o... Using the complete genome of Plasmodium falciparum 3D7 which has 14 chromosomes as an example, we have examined the distribution functions for the amount of C or G and A or T consecutively and non-overlapping blocks of m bases in this system. The function P(S) about the number of the consecutive C-G or A-T content cluster conforms to the relation P(S)∝e? ; αs values of the scaling exponent αCG are much larger than αAT; and αAT of 14 chromosomes are hardly changed, whereas αCG of 14 chromosomes have a number of fluctuations. We found maximum value of A-T cluster size is much larger than C-G, which implies the existence of large A-T cluster. Our study of the width function ξ(m) of cluster C-G content showed that follows good power law ξ(m)∝m?γ. The average γ for 14 chromosomes is 0.931. These investigations provide some insight into the nucleotide clusters of DNA sequences, and help us understand other properties of DNA sequences. 展开更多
关键词 dNA sequence Plasmodium falciparum 3d7 Nucleotide clusters Power law
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Three-dimensional motion parameters estimation from stereo image sequences based on infrared reflective markers
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作者 张灵飞 陈刚 +1 位作者 叶东 车仁生 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2011年第4期139-143,共5页
In this paper,an innovative 3D motion parameters estimation method from stereo image sequences based on infrared(IR) reflective markers is presented.It was assumed that two high speed CCD cameras had been calibrated p... In this paper,an innovative 3D motion parameters estimation method from stereo image sequences based on infrared(IR) reflective markers is presented.It was assumed that two high speed CCD cameras had been calibrated previously.The method consists of the following steps:1) the coordinate of several markers and depth map for each stereo pair was determined from the sequences of stereo images by relations of markers' coordinate the correspondence between markers was established,2) the 3D motion parameters of the target was computed based upon the matched markers' coordinate,and 3) translated 3D motion parameters estimation into the problem of least square according to the movement model of the object to be measured.Without using line,curve or corner correspondence,this method can calculate the depth of these markers feature easily and quickly in contrast to traditional approaches.The two CCD cameras work on 200 f/s,and each processing cost time is about 3 ms.It was found that,by using several markers and a large number of stereo images,this method can improve the computational speed,robustness and numerical accuracy of the motion parameters in comparison with traditional methods.The virtual simulation experiment was conducted using synthesized stereo image sequences based on 6-DOF motion platform and the experimental results proved the validity of our approach and showed that the translation and rotation precision is up to 0.1 mm and 0.1°. 展开更多
关键词 IR reflective marker 3d motion parameters stereo vision image sequence high speed CCd camera IR filter
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磁共振3D神经成像对腰骶丛显示的对比研究 被引量:3
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作者 马国骏 张建军 +3 位作者 赵秋枫 李琼 姚晔 王嵩 《医学影像学杂志》 2016年第7期1157-1159,共3页
目的探讨1.5T磁共振三维快速自旋回波成像(3D sampling perfection with application optimized contrasts by using different flip angle evolutions,3D-SPACE)与多回波融合成像(multiple echo data image combination,3D-MEDIC)序列... 目的探讨1.5T磁共振三维快速自旋回波成像(3D sampling perfection with application optimized contrasts by using different flip angle evolutions,3D-SPACE)与多回波融合成像(multiple echo data image combination,3D-MEDIC)序列对正常人腰骶丛神经的显示。方法 31例无症状正常志愿者行MRI检查,包括常规腰椎MRI、3D-SPACE及MEDIC序列扫描,原始图像传入后处理工作站行多平面图像重组。结合原始及重组图像,比较3D-SPACE及MEDIC序列图像腰骶丛神经根的信噪比及对神经的显示评分。结果 3D-MEDIC序列显示神经根的信噪比高于3D-SPACE序列(分别为70.15±24.03及28.78±7.12,P=0.000)。3D-MEDIC序列对腰骶丛的显示评分高于3D-SPACE序列(P=0.000)。结论1.5T磁共振腰骶丛神经显像中,3D-MEDIC序列优于3D-SPACE序列,可清晰显示神经根走行,是常规腰椎MRI的重要补充。 展开更多
关键词 腰骶丛 磁共振成像 3d-SPACE序列 3d-MEdIC序列
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祖先序列重建增强D-阿洛酮糖3-差向异构酶的热稳定性 被引量:2
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作者 管立军 朱玲 +7 位作者 王崑仑 李家磊 高扬 严松 张馨笛 陈晴 季妮娜 李波 《食品工业科技》 CAS 北大核心 2024年第21期121-128,共8页
为解决现有D-阿洛酮糖3-差向异构酶(DAEase)热稳定性差的产业问题,本文采用系统发育指导的大数据挖掘、合理修饰和祖先序列重建策略(ASR),重建了具有不同催化结构域DAEase的祖先序列,构建了表达载体,通过重组表达与分子对接筛选出了DAEa... 为解决现有D-阿洛酮糖3-差向异构酶(DAEase)热稳定性差的产业问题,本文采用系统发育指导的大数据挖掘、合理修饰和祖先序列重建策略(ASR),重建了具有不同催化结构域DAEase的祖先序列,构建了表达载体,通过重组表达与分子对接筛选出了DAEase A13并进行酶学性质表征,此外,还基于结构分析与分子动力学模拟揭示了DAEase A13热稳定性增强的分子机制。结果表明,基于ASR策略所构建的A13 70℃时半衰期可达8.4 h,其热稳定性较野生(WT)酶显著增强,最大转化率为31%,催化活性也略高于WT酶。立体结构模拟与分子动力学模拟揭示了ASR A13中大量氢键和疏水作用的增加维持了高温下酶分子结构的稳定性,是其热稳定性增强的主要因素。研究结果证实了ASR策略可以改造DAEase使其稳定性、活性和混杂性增强,可以为D-阿洛酮糖工业生产提供良好的生物催化剂。 展开更多
关键词 祖先序列重建 d-阿洛酮糖 d-阿洛酮糖3-差向异构酶 热稳定性
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基于3-D步行序列的双足机器人步态规划及实验研究 被引量:2
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作者 陈建芳 丁加涛 肖晓晖 《中南大学学报(自然科学版)》 EI CAS CSCD 北大核心 2018年第4期831-838,共8页
为了提高双足步行机器人对真实环境的适应能力,提出1种基于3-D步行序列的步态规划方法,模仿人类行走行为并考虑地面高度的变化。首先定义考虑足部踝关节旋转运动和机器人质心(COM)竖直高度变化的3-D步行序列。其次,在单足相阶段(SSP),... 为了提高双足步行机器人对真实环境的适应能力,提出1种基于3-D步行序列的步态规划方法,模仿人类行走行为并考虑地面高度的变化。首先定义考虑足部踝关节旋转运动和机器人质心(COM)竖直高度变化的3-D步行序列。其次,在单足相阶段(SSP),基于运动最优控制(OC)理论和线性倒立摆模型(LIMP),求解零力矩点轨迹;采用5次多项式插值,生成双足相阶段(DSP)的零力矩点轨迹,并且实现质心竖直高度变化。基于足部的运动参数,使用分段连续3次Hermite插值得到足部运动轨迹。然后,通过牛顿-拉斐逊迭代法求解关节角度解。最后,基于NAO机器人样机进行平地和倾斜地面行走的动力学仿真和样机实验。研究结果表明:本文规划方法可行,且机器人行走平稳;在平面和斜坡2种地面工况的样机实验中,足底接触力的波动幅度比相应的仿真结果分别减少37.01%和59.94%,髋关节力矩波动幅度分别减少69.13%和62.75%。 展开更多
关键词 3-d步行序列 双足机器人 步态规划 斜坡
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基于开关3-D中值滤波的视频序列去噪算法 被引量:4
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作者 曹荣 倪林 《计算机工程》 CAS CSCD 北大核心 2008年第10期232-234,共3页
为提高含噪视频序列的质量和效果,提出一种基于开关噪声检测与三维中值滤波相结合的开关3-D中值滤波算法。该算法通过判断视频序列的每一个像素点,对噪声点用3-D中值滤波处理,再对其进行二次判断,如果判为噪声点,则采用变型3-D中值滤波... 为提高含噪视频序列的质量和效果,提出一种基于开关噪声检测与三维中值滤波相结合的开关3-D中值滤波算法。该算法通过判断视频序列的每一个像素点,对噪声点用3-D中值滤波处理,再对其进行二次判断,如果判为噪声点,则采用变型3-D中值滤波处理,直到去除所有噪声点为止。仿真实验表明,该算法对"雪花"噪声有较好的滤波效果,当处理加入25%的"雪花"噪声时,3-D开关中值滤波比三维中值滤波的信噪比平均提高了10 dB左右。 展开更多
关键词 视频序列 3-d中值滤波 “雪花”噪声
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来源于瘤胃菌Ruminococcus sp.的D-阿洛酮糖3-差向异构酶的底物结合位点分析 被引量:3
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作者 张黎丽 朱玥明 +2 位作者 门燕 康振奎 孙媛霞 《微生物学通报》 CAS CSCD 北大核心 2014年第5期811-817,共7页
【目的】研究来源于瘤胃菌Ruminococcus sp.的D-阿洛酮糖3-差向异构酶的底物结合机制。【方法】通过同源模拟和同源序列比对,筛选与其底物结合相关的关键位点,进而通过定点突变构建突变体并对其动力学性质进行研究。【结果】筛选得到关... 【目的】研究来源于瘤胃菌Ruminococcus sp.的D-阿洛酮糖3-差向异构酶的底物结合机制。【方法】通过同源模拟和同源序列比对,筛选与其底物结合相关的关键位点,进而通过定点突变构建突变体并对其动力学性质进行研究。【结果】筛选得到关键位点Y6和A109,构建了突变体Y6F、Y6I、A109P及A109L。【结论】Y6既与底物结合又与催化能力相关,其-OH只与底物结合相关,芳香环则与催化能力和结合能力均相关;而A109则只是底物结合的位点。该研究结果为D-阿洛酮糖3-差向异构酶的催化机理研究及分子改造提供了借鉴。 展开更多
关键词 d-阿洛酮糖3-差向异构酶 同源模拟 序列比对 定点突变
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Radial 3D VIBE在儿童自由呼吸下腹部平扫获得T_1WI的临床应用 被引量:6
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作者 杨凯华 郑彬 《医学影像学杂志》 2018年第7期1123-1125,共3页
目的探讨Radial 3D VIBE在儿童自由呼吸下腹部平扫获得T1加权图像的临床应用。方法选取我院2015年1月~2015年8月行腹部磁共振检查患者100例,T1加权图像均采用常规梯度回波序列和Radial 3D VIBE序列分别扫描。结果对两种扫描序列定性分析... 目的探讨Radial 3D VIBE在儿童自由呼吸下腹部平扫获得T1加权图像的临床应用。方法选取我院2015年1月~2015年8月行腹部磁共振检查患者100例,T1加权图像均采用常规梯度回波序列和Radial 3D VIBE序列分别扫描。结果对两种扫描序列定性分析,结果表明Radial 3D VIBE序列得的信噪比和对比噪声比均显著高于常规序列,图像质量明显提高,能够清晰显示病变部位。与常规序列相比,Radial 3D VIBE序列出现呼吸运动伪影显著低于常规序列,P<0.05。结论 Radial 3D VIBE序列可提高磁共振成像分辨率,有效减少呼吸运动伪影,提高图像质量。 展开更多
关键词 RAdIAL 3d VIBE序列 儿童 腹部 磁共振成像
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1例RHD-CE(3-7)-D基因重组与RHCE变异型患者的血清学与分子生物学分析 被引量:2
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作者 唐炳娣 蔡仲仁 +1 位作者 邓泳诗 伍昌林 《分子诊断与治疗杂志》 2024年第6期1183-1186,1190,共5页
目的 研究分析1例Rh血型弱D、弱cE患者的血清学与分子生物学特征,为该类患者的临床安全输血提供实验依据。方法 采用微柱凝胶卡法对患者红细胞进行ABO、RhDCcEe抗原的鉴定,同时采用试管法进行血型复核,抗人球蛋白卡法筛查不规则抗体;采... 目的 研究分析1例Rh血型弱D、弱cE患者的血清学与分子生物学特征,为该类患者的临床安全输血提供实验依据。方法 采用微柱凝胶卡法对患者红细胞进行ABO、RhDCcEe抗原的鉴定,同时采用试管法进行血型复核,抗人球蛋白卡法筛查不规则抗体;采用PCR-SSP法对RhDCcEe(RhD、RhC、Rhc、RhE、Rhe)基因型进行检测;三代全长测序技术对RHD/RHCE基因序列进行测序分析。结果 微柱凝胶卡法鉴定ABO、RhD、RhCcEe血型抗原的结果为:A抗原(-)、B抗原(-)、RhD(1+)、RhC(4+)、Rhc(1+)、RhE(1+)、Rhe(4+)、对照孔(-);试管法ABO、RhD、RhCcEe抗原鉴定该患者表型为:A抗原(-)、B抗原(-)、RhD(w+)、RhC(4+)、Rhc(w+)、RhE(w+)、Rhe(4+),对照管(-);抗人球蛋白卡法筛查患者不规则抗体阴性;PCR-SSP法血型基因分型RhDCcEe结果:RhD(+)、RhC(+)、Rhc(+)、RhE(+)、Rhe(+);RHD/RHCE基因结果:RHD单倍体1为外显子1-10全缺失,而单倍体2为外显子RHD-CE基因重组融合,且确认其重组类型为RHD-CE(3-7)-D,起点在外显子2(g.20238-20312之间),终点在外显子8(g49184-50480之间),同时RHCE基因第6外显子存在新碱基点突变RHCE*cE(827C>A)。结论RHD-CE(3-7)-D基因重组融合与RHCE*cE(827C>A)新等位基因突变可能引起D、cE血型抗原弱表达,为临床安全输血提供了重要的实验数据支持。 展开更多
关键词 Rhd/cE弱抗原 RHd-CE(3-7)-d重组 RHCE*cE(827C>A) 三代全长测序
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An interactive 3D viewer of molecules compatible with the suite of ANTHEPROT programs 被引量:2
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作者 Gilbert Deléage 《Journal of Biophysical Chemistry》 2012年第1期35-38,共4页
In this paper, I will describe a completely new 3D module which can be called from within the well known ANTHEPROT program devoted to protein sequences analysis. This module allows fully interactive handling of high-q... In this paper, I will describe a completely new 3D module which can be called from within the well known ANTHEPROT program devoted to protein sequences analysis. This module allows fully interactive handling of high-quality 3D structures with various modes of representation (CA sticks, wireframe, ball and sticks, spacefill mod-els as well as surface, ribbons, Ramachandran plots). Alternatively, ANTHEPROT 3D can be used as an external program fully independant from the global package. It is available from the download page of the web site (http://antheprot-pbil.ibcp.fr/). More than 2800 downloads last year were recorded since the program was delivered. 展开更多
关键词 Protein sequence Analysis Structural BIOINFORMATICS Software 3d Structures
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MR Visualization of Spinal Dural Arterio-Venous Fistula Using T2-Weighted 3D SPACE—A Spin-Echo Technique 被引量:1
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作者 Franz A. Fellner Christine M. Fellner +1 位作者 Rene Chapot Kaveh Akbari 《Journal of Biomedical Science and Engineering》 2015年第5期327-332,共6页
Spinal dural arteriovenous fistulas (SDAVF) account for about 70% of all spinal AV shunts. We re-port on a 56-year-old male patient with progressive neurological symptoms. Pre- and post-contrast MR imaging of the spin... Spinal dural arteriovenous fistulas (SDAVF) account for about 70% of all spinal AV shunts. We re-port on a 56-year-old male patient with progressive neurological symptoms. Pre- and post-contrast MR imaging of the spine, including contrast-enhanced MR angiography was done on a 1.5 T MR scanner. In addition a special (pre-contrast) 3D turbo-spin-echo sequence with variable flip angle evolution (SPACE) was performed providing a slice thickness of 0.9 mm. T2-weighted images showed extensive widened and tortuous vascular structures with typical flow voids intradurally due to dilated pial veins. The origin of the spinal dural arteriovenous fistula was found to be the radiculomeningeal artery at level D10/11 on the left side, visualized by the T2-weighted 3D SPACE sequence and CE MRA. After imaging the patient was referred to an interventional neuroradiology department for therapy. With the detailed information from MR imaging concerning the fistula level the interventional radiologist was able to find the fistula immediately and consequently treated it successfully by endovascular therapy. Interpreting the MR images of this obvious case of a SDAVF, SPACE sequence after post processing with 3D MPR software directly led to the location of the AV shunt at D10/11 on the left side, better than 3D contrast-enhanced MR angiography did. Therefore, SPACE can be a useful adjunct in further evaluation of spinal dural arteriovenous fistula and especially in defining fistula level. 展开更多
关键词 Contrast Enhanced Magnetic Resonance ANGIOGRAPHY (CE MRA) 3d Pulse sequences 3d SPACE SPINAL dURAL Arterio-Venous FISTULA (SdAVF)
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基于3D卷积的视频错帧筛选方法
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作者 缪宇杰 吴智钧 宫婧 《计算机技术与发展》 2018年第5期179-181,186,共4页
为了提取更好的视频特征,提高训练精准度,提出了一个基于CNN(convolutional neural network,卷积神经网络)的错帧筛选模型。所谓错帧,是指在时间上乱序的帧序列,相反,有序帧是指遵守时间顺序的帧序列。其目标是从若干组帧序列中,筛选出... 为了提取更好的视频特征,提高训练精准度,提出了一个基于CNN(convolutional neural network,卷积神经网络)的错帧筛选模型。所谓错帧,是指在时间上乱序的帧序列,相反,有序帧是指遵守时间顺序的帧序列。其目标是从若干组帧序列中,筛选出顺序错误的一组帧序列。采用无监督学习的方法来训练模型,因此不需要依赖有标签的数据集。基于这个模型的目标以及无标签的训练方式,采用了一个多分支的CNN结构,并且是端到端的。其输入的若干组帧序列从视频中采样获得,分别进行3D卷积编码后,能够提取出每组帧序列在时间和空间上的特征。为了找出帧顺序有误的一组序列,该模型对每组帧序列进行对比,找出它们之间的共同规则,从而筛选出违背此规则的那一组序列。在UCF101数据集上的实验结果证实了该方法的有效性,错帧筛选的准确率高。 展开更多
关键词 无监督学习 卷积神经网络 错帧筛选 3d卷积
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A开发区D区块BⅠ33^2单元的地层格架建立与初步认识
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作者 袁琳 《长江大学学报(自科版)(中旬)》 CAS 2014年第5期10-12,共3页
A开发区2010年以高分辨率层序地层学理论为指导,重构了A开发区BⅠ1-3沉积体系,研究发现C区块及其以北BⅠ323与D区块BⅠ323单元在油层组中的相对位置明显不同,原对比存在"窜层"。提出了D-E区块存在BⅠ32b3单元,并重新认识了BⅠ... A开发区2010年以高分辨率层序地层学理论为指导,重构了A开发区BⅠ1-3沉积体系,研究发现C区块及其以北BⅠ323与D区块BⅠ323单元在油层组中的相对位置明显不同,原对比存在"窜层"。提出了D-E区块存在BⅠ32b3单元,并重新认识了BⅠ32b3单元储层发育特征。以该成果为指导,对D区块BⅠ33单元进行精细解剖,确定了BⅠ32b3单元在该区的平面分布范围和砂体平面分布特征,确保了该区BⅠ33划分标准与全区的统一。 展开更多
关键词 A开发区 d区块 BⅠ33^2单元 储层发育特征 平面分布 砂体平面分布
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Cloning and analysis of [NiFe]-hydrogenase genes from Arthrospira platensis FACHB341
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作者 张晓辉 Sui Zhenghong +2 位作者 Yoshihiro Shiraiwa Koji Iwamoto Zhang Xuecheng 《High Technology Letters》 EI CAS 2005年第4期421-426,共6页
The complete [ NiFe]-hydrogenase genes were cloned from cyanobacterium, Arthrospira platensis FACHB341, using PCR and in-vitro cloning. The total length is 2078bp, including hoxY 549bp, hoxH 1431bp and the spacer in b... The complete [ NiFe]-hydrogenase genes were cloned from cyanobacterium, Arthrospira platensis FACHB341, using PCR and in-vitro cloning. The total length is 2078bp, including hoxY 549bp, hoxH 1431bp and the spacer in between 98bp. There is a stem-loop structure, downstream of the hoxY gene, serving as a transcription terminator. The deduced amino acid sequences of HoxY aud HoxH consist of acidic amino acid of 15.8% and 12.0%; alkalitropic amino acid of 11.9% and 15.3%; hydrophobic amino acid of 46.3% and 41.0%, respectively. The similarities of hoxY and hoxH genes in Arthrospira platensis FACHB341 to their homologues in other cyanobacteria were compared respectively. The secondary structures and 3D models of small and large subunits of [ NiFe]-hydrogenase were predicted by using 3D-PSSM. 展开更多
关键词 Arthrospira platersis [NiFe]-Hydrogenase in-vitro cloning sequence analysis secondary structure 3d model
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数列{D(u,v)=u^(3)+v^(3),u,v∈N}的若干性质
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作者 马咸礼 《青海师范大学学报(自然科学版)》 2007年第2期18-20,共3页
本文给出了数列{D(u,v)=u^(3)+v^(3),u,v∈N}的定义及其若干性质.
关键词 数列{d(u v)=u^(3)+v^(3) u v∈N} 行列式 数列{E(u v)=vd(u v)}
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Isolation and Identification of Staphylococcus chromogenes and 3 D Structural Analysis of the katA Gene
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作者 HU Xiu-cai LV Ai-jun +3 位作者 WANG Yi LI Xue MING Qing-lei ZHUAi-hua 《Animal Husbandry and Feed Science》 CAS 2013年第4期192-197,共6页
[Objective]Staphylococcus arthritis became an increasingly significant health problem in intensive chicken farming in China.[Method]In this study,a bacteria strain was isolated from the broiler chicken suffering from ... [Objective]Staphylococcus arthritis became an increasingly significant health problem in intensive chicken farming in China.[Method]In this study,a bacteria strain was isolated from the broiler chicken suffering from arthritis and named as the strain Gg1.[Result]It was then identified as Staphylococcus chromogenes by the biochemical tests and phylogenetic tree analysis based on 16S rDNA sequence.Furthermore,the catalase(katA)gene was amplified by PCR using the designed primers,and the expected fragment was 1 232 bp long encoding a protein of 410 amino acids that shares the conserved motifs including catalase,heme-binding ligand and active center motif.Six phosphorylation sites(Ser95,Thr96,Ser241,Ser242,Thr281,Ser338),four conserved residues(Ser95,His216,Tyr281,Asp341)and two active sites(His56,Asn129)were demonstrated by multiple sequence alignment and homology comparisons.The homology modeling of 3D structure of katA protein was done by SWISSMODEL server based on the template retrieved from the catalase(PDB:2ISA_A)of Vibrio salmonicida.The katA protein represents a four-domain globular protein,the quality and reliability of the resulting protein structure was further verified by Ramachandran plot.[Conclusion]To our knowledge,this is the first report of S.chromogenes linked to arthritis in chicken and the bioinformatic characterization of its katA gene. 展开更多
关键词 Staphylococcus chromogenes Isolation and identification katA sequence analysis 3d structure
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千里光热激蛋白90-3(Hsp90-3)的生物信息学与功能分析 被引量:2
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作者 平军娇 张珍 +2 位作者 蔡振锋 汤贤春 钱刚 《植物科学学报》 CAS CSCD 北大核心 2012年第4期385-393,共9页
Hsp90是真核细胞重要的一类分子伴侣,与植物的生长发育、抗逆性、信号转导及生物进化等功能密切相关。为了深入理解高等植物Hsp90结构与功能的关系,该研究从千里光(Senecio scandens)全长cDNA文库中分离到Hsp90-3基因。序列分析结果表明... Hsp90是真核细胞重要的一类分子伴侣,与植物的生长发育、抗逆性、信号转导及生物进化等功能密切相关。为了深入理解高等植物Hsp90结构与功能的关系,该研究从千里光(Senecio scandens)全长cDNA文库中分离到Hsp90-3基因。序列分析结果表明,该基因编码699个氨基酸的多肽,与拟南芥(Arabidopsis thaliana)AtHsp90-3(登录号:NP_200412.1)的同源性最高,为93.71%;预测蛋白质的分子量为79.78 kD,理论等电点为5.08。信号序列分析结果发现,该蛋白主要定位于细胞的细胞核、过氧化物酶体、叶绿体类囊体膜及叶绿体基质中,提示作为分子伴侣,高等植物Hsp90-3参与细胞内膜系统蛋白质的转运。结构与功能分析发现,该蛋白有3个结构域及1个连接区,推测Hsp90-3在真核细胞的信号转导、转录调控及胁迫表达等过程中发挥重要功能。 展开更多
关键词 千里光 Hsp90-3基因 结构预测 序列分析
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