Objective： To explore and investigate the selection of effective antisense oligodeoxynuleotides with the help of computer and RNAstructure folding software. Methods： Bcl-2 gene was used as the target gene and five a...Objective： To explore and investigate the selection of effective antisense oligodeoxynuleotides with the help of computer and RNAstructure folding software. Methods： Bcl-2 gene was used as the target gene and five antisense oligodeoxynuleotides were designed to be bound to Bcl-2 mRNA optimal secondary structure regions that were predicted free from intramolecular fold or instability of free energy. The five antisense oligodeoxynucleotides were studied with experimental assay of leukemia cells, including cell grow assay with tropan blue exclusion, expression of Bcl-2 protein detected with immunochemistry and flowcytometry, Bcl-2 mRNA content detected with RT-PCR technique, as well as apoptosis observed and determined with morphonological method, electrophoresis and flowcytometry. Results： The results showed that two of the five antisense oligodeoxynucleotides were effective antisense oligodeoxynucleotides, which were able to inhibit cell growth in leukemia, to decrease the level of Bcl-2 mRNA and protein, to induce apoptosis of leukemia cells significantly. Conclusion： The computational prediction of antisense efficacy is faster than other methods and more efficient, which can potentially speed the development of sequences for both research and clinical applications.展开更多
In this paper,we consider the initial boundary value problem for the 2-D hyperbolic viscous Cahn-Hilliard equation.Firstly,we prove the existence and uniqueness of the local solution by the Galerkin method and contrac...In this paper,we consider the initial boundary value problem for the 2-D hyperbolic viscous Cahn-Hilliard equation.Firstly,we prove the existence and uniqueness of the local solution by the Galerkin method and contraction mapping principle.Then,using the potential well theory,we study the global well-posedness of the solution with initial data at different levels of initial energy,i.e.,subcritical initial energy,critical initial energy and arbitrary positive initial energy.For subcritical initial energy,we prove the global existence,asymptotic behavior and finite time blowup of the solution.Moreover,we extend these results to the critical initial energy using the scaling technique.For arbitrary positive initial energy,including the sup-critical initial energy,we obtain the sufficient conditions for finite time blow-up of the solution.As a further study for estimating the blowup time,we give a unified expression of the lower bound of blowup time for all three initial energy levels and estimate the upper bound of blowup time for subcritical and critical initial energy.展开更多
文摘Objective： To explore and investigate the selection of effective antisense oligodeoxynuleotides with the help of computer and RNAstructure folding software. Methods： Bcl-2 gene was used as the target gene and five antisense oligodeoxynuleotides were designed to be bound to Bcl-2 mRNA optimal secondary structure regions that were predicted free from intramolecular fold or instability of free energy. The five antisense oligodeoxynucleotides were studied with experimental assay of leukemia cells, including cell grow assay with tropan blue exclusion, expression of Bcl-2 protein detected with immunochemistry and flowcytometry, Bcl-2 mRNA content detected with RT-PCR technique, as well as apoptosis observed and determined with morphonological method, electrophoresis and flowcytometry. Results： The results showed that two of the five antisense oligodeoxynucleotides were effective antisense oligodeoxynucleotides, which were able to inhibit cell growth in leukemia, to decrease the level of Bcl-2 mRNA and protein, to induce apoptosis of leukemia cells significantly. Conclusion： The computational prediction of antisense efficacy is faster than other methods and more efficient, which can potentially speed the development of sequences for both research and clinical applications.
基金supported by the NSFC(12271122)the Fundamental Research Funds for the Central Universities.Han’s research was supported by the Fundamental Research Funds for the Central Universities(3072023GIP2401).
文摘In this paper,we consider the initial boundary value problem for the 2-D hyperbolic viscous Cahn-Hilliard equation.Firstly,we prove the existence and uniqueness of the local solution by the Galerkin method and contraction mapping principle.Then,using the potential well theory,we study the global well-posedness of the solution with initial data at different levels of initial energy,i.e.,subcritical initial energy,critical initial energy and arbitrary positive initial energy.For subcritical initial energy,we prove the global existence,asymptotic behavior and finite time blowup of the solution.Moreover,we extend these results to the critical initial energy using the scaling technique.For arbitrary positive initial energy,including the sup-critical initial energy,we obtain the sufficient conditions for finite time blow-up of the solution.As a further study for estimating the blowup time,we give a unified expression of the lower bound of blowup time for all three initial energy levels and estimate the upper bound of blowup time for subcritical and critical initial energy.
