Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation a...Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation and minimal toxicity.Selenium-enriched yeast(SeY)is a well-established source,while selenium-enriched lactobacilli(SeL),a newer alternative,offers the added benefits of probiotics.This study examined the effects of SeY and SeL on egg quality,antioxidant capacity,Se deposition,and gut health in laying hens.After a two-week pre-treatment with a Sedeficient diet(SeD),450 Hy-Line Brown laying hens(30-week-old)were assigned into five dietary groups with six replicates of 15 hens each.The groups included a SeD,SeD supplemented with 1.5 mg Se/kg from SeY(SeY15),or 1.5,3.0,and 6.0 mg Se/kg from SeL(SeL15,SeL30,SeL60).The feeding trial lasted for 12 weeks.Results SeY15 and SeL15 improved the feed-to-egg ratio(P<0.05)in the latter stages.Haugh units were significantly increased(P<0.05)in the SeY15 and SeL30 groups,while darker yolk color(P<0.05)was observed in the SeY15,SeL15,and SeL60 groups.All Se-supplemented diets increased Se content in whole eggs,albumen,and yolk(P<0.05),while SeL groups showed a dose-dependent effect.Antioxidant enzyme activities increased,and MDA content decreased in the serum(P<0.05),with SeY15 showing the highest GSH-Px levels(P<0.05).SeL60 increased serum alkaline phosphatase and aspartate transaminase,and distorted the liver architecture(P<0.05).Se-diets reduced concentrations of reactive oxygen species(ROS)in the ileum and liver(P<0.05).SeL15 improved the ileal villus height-tocrypt depth ratio(P<0.05).SeY15 and/or SeL15 up-regulated TXNRD1 and SEPHS1 mRNA while down-regulating SCLY expression in the liver.SeY15 altered ileal microbiota by increasing both beneficial and pathogenic bacteria,whereas SeL15 predominantly boosted beneficial bacteria.Conclusion SeL integrates the antioxidant properties of organic Se with the probiotic benefits on gut health,resulting in a performance-enhancing effect comparable to that of SeY.However,high SeL level(6.0 mg Se/kg)compromised productivity and metabolic functions while enhancing Se deposition.展开更多
Selenium is one of the important trace elements in the human body.Its deficiency will directly affect human health.With people's attention to health,the content of selenium in food has gradually attracted attentio...Selenium is one of the important trace elements in the human body.Its deficiency will directly affect human health.With people's attention to health,the content of selenium in food has gradually attracted attention.However,detecting selenium compounds in complex samples remains a challenge.In this work,we built an online heating-reaction device.This device combines the electrospray extraction ionization mass spectrometry(EESI-MS)with the heating reaction device,which can simultaneously detect various selenium compounds in complex liquid samples.Under acidic conditions,the sample was heated and catalyzed by a heating reaction device,so that the SeO~(2-)_(3)and O-phenylenediamine(OPD)could generate 1,3-dihydro-2,1,3-benzoselenadiazole.Based on the above reactions,we can detect organic selenium,inorganic selenium and other compounds in liquid samples by organic mass spectrometry.In this experiment,we determined the content of three forms of selenium:selenomethionine(SeMet),l-selenocystine(SeCys(2)),and sodium selenite.The calibration curves for SeMet,SeCys(2),and sodium selenite showed strong linearity within a range of 0.50-50.00μg/L.The limits of detection(LOD)for the three compounds were 0.22,0.27,and 0.41μg/L,respectively.The limits of quantification(LOQ)were 0.68,0.81,and 1.23μg/L,respectively.Spiked recoveries at three levels ranged from 98.8%to 106.1%.In addition,this method can simultaneously detect three selenium compounds and three other specific chemical components in tea infusion samples,providing a rapid and efficient method for identifying tea quality.展开更多
The effects of long-term moisture changes on the migration,release,and bioavailability of selenium in soil are complex.Due to the lack of effective monitoring methods for precise quantification,its dynamic behavior is...The effects of long-term moisture changes on the migration,release,and bioavailability of selenium in soil are complex.Due to the lack of effective monitoring methods for precise quantification,its dynamic behavior is still unclear.Based on the DGT(Diffusive Gradients in Thin-films)technology,this study sets up three moisture control scenarios:continuous wet,wet-dry alternating,and continuous dry,and carries out a 6-month soil moisture control experiment.In the experiment,the DGT device collected the diffusion gradient data of soil selenium under different scenarios,and analyzed the migration characteristics of selenium in combination with the adsorption isotherm.Meanwhile,the release rate,migration coefficient,and bioavailability parameters of selenium are calculated by fitting the first-order kinetic model,further verifying the reliability and applicability of the DGT data.The experimental results demonstrate that under continuous wet conditions,the release rate of soil selenium reaches 1.85µg·cm^(-2)·h^(-1),with a migration coefficient of 0.012 cm^(2)·h^(-1)and a bioavailability parameter of 0.74;under wet-dry alternating conditions,they are 1.42µg·cm^(-2)·h^(-1),0.01 cm^(2)·h^(-1),and 0.68,respectively;under continuous dry conditions,the release rate of soil selenium is the smallest,at 0.88µg·cm^(-2)·h^(-1),with a migration coefficient of 0.004 cm^(-2)·h^(-1)and a bioavailability parameter of 0.5.The results of this experiment reveal the dynamic behavior of soil selenium under different moisture conditions and reflect the high efficiency of DGT technology in dynamic monitoring and quantitative analysis of soil selenium behavior,providing a scientific basis for the optimal management of rhizosphere soil selenium.展开更多
Bisphenol A(BPA)is an industrial pollutant that can cause immune impairment.Selenium acts as an antioxidant,as selenium deficiency often accompanies oxidative stress,resulting in organ damage.This study is the first t...Bisphenol A(BPA)is an industrial pollutant that can cause immune impairment.Selenium acts as an antioxidant,as selenium deficiency often accompanies oxidative stress,resulting in organ damage.This study is the first to demonstrate that BPA and/or selenium deficiency induce pyroptosis and ferroptosis-mediated thymic injury in chicken and chicken lymphoma cell(MDCC-MSB-1)via oxidative stress-induced endoplasmic reticulum(ER)stress.We established a broiler chicken model of BPA and/or selenium deficiency exposure and collected thymus samples as research subjects after 42 days.The results demonstrated that BPA or selenium deficiency led to a decrease in antioxidant enzyme activities(T-AOC,CAT,and GSH-Px),accumulation of peroxides(H2O2 and MDA),significant upregulation of ER stress-relatedmarkers(GRP78,IER 1,PERK,EIF-2α,ATF4,and CHOP),a significant increase in iron ion levels,significant upregulation of pyroptosis-related gene(NLRP3,ASC,Caspase1,GSDMD,IL-18 and IL-1β),significantly increase ferroptosis-related genes(TFRC,COX2)and downregulate GPX4,HO-1,FTH,NADPH.In vitro experiments conducted in MDCC-MSB-1 cells confirmed the results,demonstrating that the addition of antioxidant(NAC),ER stress inhibitor(TUDCA)and pyroptosis inhibitor(Vx765)alleviated oxidative stress,endoplasmic reticulum stress,pyroptosis,and ferroptosis.Overall,this study concludes that the combined effects of oxidative stress and ER stress mediate pyroptosis and ferroptosis in chicken thymus induced by BPA exposure and selenium deficiency.展开更多
Selenium is an essential trace element for human beings and it plays a significant role for the health of human nervous system.The strong antioxidant effect of selenium endows the element with the ability to treat var...Selenium is an essential trace element for human beings and it plays a significant role for the health of human nervous system.The strong antioxidant effect of selenium endows the element with the ability to treat various diseases,including Alzheimer's disease(AD).In the body,selenium exists in the forms of selenoproteins,which could treat AD through various pathways,such as inhibiting peroxidation,inhibiting apoptosis signal pathway,reducing the levels of Aβin neurons and alleviating Tau protein caused by pathological damage.This article aims to comprehensively elaborate on the relationship between selenium and AD.展开更多
With the wide application of thrombolytic drugs and the advancement of endovascular therapeutic techniques, the recanalization treatment of acute artery occlusion in ischemic stroke (IS) has made a leap forward, but i...With the wide application of thrombolytic drugs and the advancement of endovascular therapeutic techniques, the recanalization treatment of acute artery occlusion in ischemic stroke (IS) has made a leap forward, but ischemic brain tissues still face ischemia-reperfusion injury after recanalization. Nowadays, effective neurological protective agents still cannot completely resist the multiple damages of ischemia-reperfusion injury. As an iron-dependent mode of programmed cell death, ferroptosis occupies an important position in ischemia-reperfusion injury. Selenium plays a unique protective role in ischemia-reperfusion injury as an active site element in the center of glutathione peroxidase. Therefore, the study mainly aims to review the protective role of selenium in IS and the related mechanisms, as well as the effect of selenium on the risk factors of IS.展开更多
The Kirschner wire(K-wire)is widely used in orthopedic external fixation due to its versatility and clinical effectiveness.However,a significant challenge associated with its use is the potential for bacterial migrati...The Kirschner wire(K-wire)is widely used in orthopedic external fixation due to its versatility and clinical effectiveness.However,a significant challenge associated with its use is the potential for bacterial migration,subsequent infection,and dislodgement as the wire penetrates the skin and bone.This study introduces a novel bioactive material,selenium/calcium silicate(Se/β-CS),achieved by integrating selenium-an essential trace element in the human body-into bioceramic calcium silicate.This integration was accomplished using a combined chemical co-deposition method and redox reaction.Furthermore,a uniform and controllable Se/β-CS coating was applied to the K-wire's surface using the Langmuir-Blodgett technique.This coating gradually releases active components-Si,Ca,and Se-that effectively eliminate bacterial infections and promote osteointegration.The findings of this study offer promising opportunities for the use of robust and multifunctional coating materials on implantable devices,particularly within the fields of orthopedics,transplantation,and surgery.展开更多
The erosion process and kinetics of PbTe particles in a selenium melt were investigated.The results reveal that the limiting step of the reaction is controlled by product layer diffusion and the interfacial chemical r...The erosion process and kinetics of PbTe particles in a selenium melt were investigated.The results reveal that the limiting step of the reaction is controlled by product layer diffusion and the interfacial chemical reaction at low temperatures(573,583,and 593 K),but the limiting step is controlled by boundary layer diffusion at high temperatures(603 and 613 K).The Se-and Te-atom diffusion in the product layer becomes unbalanced as the product layer thickens,with Kirkendall voids generating in the product layer accelerating PbTe particle erosion.After the PbTe impurities in the selenium melt evolve into PbSe and Te,Te is evenly distributed in the selenium melt owing to the solubility of Se and Te.This study serves to clarify the evolution behavior of PbTe impurities in the selenium melt and the reason that Te often occurs in Se.展开更多
Selenium distillation slag(SDS)is a high-value-added secondary resource with a high recovery value.This paper aims to investigate the leaching behavior and kinetics of selenium,tellurium,and copper in the SDS acid oxi...Selenium distillation slag(SDS)is a high-value-added secondary resource with a high recovery value.This paper aims to investigate the leaching behavior and kinetics of selenium,tellurium,and copper in the SDS acid oxidation leaching process with H_(2)SO_(4) and H_(2)O_(2).The experimental results showed that under the optimum conditions,the contents of selenium,tellurium,and copper in the SDS were reduced from 22.13 wt%,3.58 wt%,and 6.42 wt%to 3.06 wt%,0.27 wt%,and 0.33 wt%,respectively.Correspondingly,the recovery rates are 87.08%,97.15%and 99.7%.The leaching processes of selenium and tellurium were controlled by diffusion and chemical reactions,and the leaching behavior of copper was controlled by chemical reactions.Below 45℃,the activation energies for selenium,tellurium,and copper were found to be 26.47,62.18 and 19.67 kJ/mol,respectively.In addition,the contents of lead,silver and gold in the leaching residue are increased to 46.8 wt%,8.35 wt%and 0.27 wt%,respectively.These substances can be utilized as raw materials for the recovery of these valuable metals.Importantly,the entire process does not generate toxic or harmful waste,making it a green and environmentally friendly method for resource recovery.展开更多
Wound dressings with tissue adhesion,good mechanical,antioxidant and anti-inflammatory performance are urgently needed.In this work,we present a multifunctional selenium nanoparticles(SeNPs)/citric acid/gelatin/hydrox...Wound dressings with tissue adhesion,good mechanical,antioxidant and anti-inflammatory performance are urgently needed.In this work,we present a multifunctional selenium nanoparticles(SeNPs)/citric acid/gelatin/hydroxysuccinimide-grafted polyacrylic acid nanocomposite hydrogel adhesive(SCA) specifically designed for wound healing applications.The SCA was prepared via a one-pot processing,where SeNPs synthesized via chemical reduction were incorporated.These SeNPs not only endowed SCA with robust wet adhesion ability,excellent stretchability,and skin-matched elasticity modulus by serving as a physical crosslinker to modulate swelling equilibrium and molecular slippage,but also enhanced the biocompatibility and free radical scavenging capacity of SCA.Furthermore,in vivo evaluation of full-thickness cutaneous defects of rats revealed that SCA effectively reduced inflammation,promoted wound closure,and increased collagen deposition.All these results demonstrated that the developed SCA offers a promising therapeutic strategy for wound healing applications.展开更多
The trace element selenium(Se)occurs naturally throughout the earth.Se deficiency has been linked to impaired breast health and other diseases in human and animals.Compared to severe Se deficiency,marginal dietary Se ...The trace element selenium(Se)occurs naturally throughout the earth.Se deficiency has been linked to impaired breast health and other diseases in human and animals.Compared to severe Se deficiency,marginal dietary Se deficiency accusers more frequently in low-Se regions.Therefore,to investigate the Se status and inflammatory response of the mammary gland under marginal dietary Se levels,an lipopolysaccharide(LPS)induced mouse mastitis model was established.Mice were fed with moderate Se diet(0.087 mg•kg^(-1) Se),adequate Se diet(0.15 mg•kg^(-1) Se)or excessive Se diet(1.5 mg•kg^(-1) Se)for 60 days.Se status and inflammatory factors were investigated.Results showed that the Se status of mammary gland correlated with dietary Se levels.Marginal Se deficiency exacerbated mammary tissue histopathology;increased the mRNA level of inflammatory genes tumor necrosis factor alpha(TNF-α),interleukin-1β(IL-1β)and cyclooxygenase-2(COX-2);and enhanced the phosphorylation of NF-κB p65 in mammary gland tissues.Supplementation of Se in diet higher than recommended levels reduced the inflammatory reaction of mammary glands in LPS-induced mastitis model and provided a protective effect.展开更多
Selenium nanoparticles(SeNPs)are increasingly recognized for their exceptional antibacterial properties.This study aimed to develop a green,safe,and efficient method for the biosynthesis of SeNPs using the fungus Euro...Selenium nanoparticles(SeNPs)are increasingly recognized for their exceptional antibacterial properties.This study aimed to develop a green,safe,and efficient method for the biosynthesis of SeNPs using the fungus Eurotium cristatum,a novel approach in SeNP synthesis.The process yielded(36.40±4.22)mg of SeNPs per liter of 1.2 mmol/L sodium selenite supplementation.These SeNPs exhibited an average diameter of 231.7 nm and a negative charge,and they remained stable when stored at 4℃.Ultraviolet and visible spectrophotometry revealed a maximum absorption peak at 212 nm,suggesting effective nanoparticle formation.Fourier transform infrared spectrometry indicated that proteins and carbohydrates in the mycelium contributed to the SeNP synthesis.Concentrations of SeNPs below 50μg Se/mL did not exhibit cytotoxic effects on the growth and proliferation of human hepatocyte L-02 cells.The minimum inhibitory concentration of SeNPs was found to be 2 mg/mL against both methicillin-resistant Staphylococcus aureus(Gram-positive)and Escherichia coli(Gram-negative).The SeNPs compromised the cellular integrity of test strains,causing leakage of intracellular contents and disruption of the oxidative stress system,leading to irreversible damage.Our results demonstrate the potential of SeNPs biosynthesized by E.cristatum to act as effective antibacterial agents,signifying a novel and promising approach to developing natural antimicrobial solutions.展开更多
Selenium is a crucial trace element that contributes to physiological processes in the body as selenoproteins.Selenoproteins serve as an integral role in the body in controlling the redox state of cells and protecting...Selenium is a crucial trace element that contributes to physiological processes in the body as selenoproteins.Selenoproteins serve as an integral role in the body in controlling the redox state of cells and protecting against damage induced by oxidative stress.This study aimed to investigate the effects and possible mechanism of selenium on selenoproteins expression in EA.hy926 cells induced by oxidized low density lipoprotein(oxLDL).The impact of selenium on the viability of EA.hy926 cells was detected by the methylthiazolyldiphenyltetrazolium bromide(MTT)method,and intracellular reactive oxygen species(ROS)level and mitochondrial membrane potential were assessed by fluorescent probe DCFH-DA and JC-1,respectively.RNA-seq,quantitative real-time polymerase chain reaction(qPCR),and Western blot were used to investigate the selenoprotein expression.Selenoprotein mRNA translation efficiency was analyzed by ribosome profiling(Ribo-Seq)coupled with transcriptomics.Our data showed that selenium supplementation(0.5μmol/L)significantly decreased ROS production,increased mitochondrial inner membrane potential and increased the proliferative activity of EA.hy926 cells induced by oxLDL.Moreover,The protective effects of selenium against oxLDL-induced EA.hy926 cell injury were associated with the upregulation of the expressions of selenoproteins glutathione peroxidase 1(GPX1),glutathione peroxidase 4(GPX4),and thioredoxin reductase 1(TXNRD1).Furthermore,the expressions of selenoproteins GPX1 and GPX4 were hierarchically controlled,but the expressions of selenoproteins TXNRD1 were mainly regulated by oxLDL.Finally,Ribo-Seq coupled with transcriptomics results demonstrated that the expressions of selenoproteins GPX1,GPX4,and TXNRD1 were regulated at the translation process level.These findings suggested that selenium could have preventive effects in oxLDL induced EA.hy926 cell injury by regulating the selenoprotein expression,and the selenoproteins expressions at the translation level in vascular endothelial cells need further study.展开更多
基金supported by the National Natural Science Foundation of China(32302774)Beijing Innovation Consortium of Agriculture Research System(BAIC04)+1 种基金China Agriculture Research System(CARS-40)the Agricultural Science and Technology Innovation Program of the Feed Research Institute of the Chinese Academy of Agricultural Sciences(CAASIFR-ZDRW202402).
文摘Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation and minimal toxicity.Selenium-enriched yeast(SeY)is a well-established source,while selenium-enriched lactobacilli(SeL),a newer alternative,offers the added benefits of probiotics.This study examined the effects of SeY and SeL on egg quality,antioxidant capacity,Se deposition,and gut health in laying hens.After a two-week pre-treatment with a Sedeficient diet(SeD),450 Hy-Line Brown laying hens(30-week-old)were assigned into five dietary groups with six replicates of 15 hens each.The groups included a SeD,SeD supplemented with 1.5 mg Se/kg from SeY(SeY15),or 1.5,3.0,and 6.0 mg Se/kg from SeL(SeL15,SeL30,SeL60).The feeding trial lasted for 12 weeks.Results SeY15 and SeL15 improved the feed-to-egg ratio(P<0.05)in the latter stages.Haugh units were significantly increased(P<0.05)in the SeY15 and SeL30 groups,while darker yolk color(P<0.05)was observed in the SeY15,SeL15,and SeL60 groups.All Se-supplemented diets increased Se content in whole eggs,albumen,and yolk(P<0.05),while SeL groups showed a dose-dependent effect.Antioxidant enzyme activities increased,and MDA content decreased in the serum(P<0.05),with SeY15 showing the highest GSH-Px levels(P<0.05).SeL60 increased serum alkaline phosphatase and aspartate transaminase,and distorted the liver architecture(P<0.05).Se-diets reduced concentrations of reactive oxygen species(ROS)in the ileum and liver(P<0.05).SeL15 improved the ileal villus height-tocrypt depth ratio(P<0.05).SeY15 and/or SeL15 up-regulated TXNRD1 and SEPHS1 mRNA while down-regulating SCLY expression in the liver.SeY15 altered ileal microbiota by increasing both beneficial and pathogenic bacteria,whereas SeL15 predominantly boosted beneficial bacteria.Conclusion SeL integrates the antioxidant properties of organic Se with the probiotic benefits on gut health,resulting in a performance-enhancing effect comparable to that of SeY.However,high SeL level(6.0 mg Se/kg)compromised productivity and metabolic functions while enhancing Se deposition.
基金financially supported by Jiangxi University of Chinese Medicine School-level Science and Technology Innovation Team Development Program(No.CXTD22005)PhD research startup fund of Jiangxi University of Chinese Medicine(No.2023BSZR005)。
文摘Selenium is one of the important trace elements in the human body.Its deficiency will directly affect human health.With people's attention to health,the content of selenium in food has gradually attracted attention.However,detecting selenium compounds in complex samples remains a challenge.In this work,we built an online heating-reaction device.This device combines the electrospray extraction ionization mass spectrometry(EESI-MS)with the heating reaction device,which can simultaneously detect various selenium compounds in complex liquid samples.Under acidic conditions,the sample was heated and catalyzed by a heating reaction device,so that the SeO~(2-)_(3)and O-phenylenediamine(OPD)could generate 1,3-dihydro-2,1,3-benzoselenadiazole.Based on the above reactions,we can detect organic selenium,inorganic selenium and other compounds in liquid samples by organic mass spectrometry.In this experiment,we determined the content of three forms of selenium:selenomethionine(SeMet),l-selenocystine(SeCys(2)),and sodium selenite.The calibration curves for SeMet,SeCys(2),and sodium selenite showed strong linearity within a range of 0.50-50.00μg/L.The limits of detection(LOD)for the three compounds were 0.22,0.27,and 0.41μg/L,respectively.The limits of quantification(LOQ)were 0.68,0.81,and 1.23μg/L,respectively.Spiked recoveries at three levels ranged from 98.8%to 106.1%.In addition,this method can simultaneously detect three selenium compounds and three other specific chemical components in tea infusion samples,providing a rapid and efficient method for identifying tea quality.
文摘The effects of long-term moisture changes on the migration,release,and bioavailability of selenium in soil are complex.Due to the lack of effective monitoring methods for precise quantification,its dynamic behavior is still unclear.Based on the DGT(Diffusive Gradients in Thin-films)technology,this study sets up three moisture control scenarios:continuous wet,wet-dry alternating,and continuous dry,and carries out a 6-month soil moisture control experiment.In the experiment,the DGT device collected the diffusion gradient data of soil selenium under different scenarios,and analyzed the migration characteristics of selenium in combination with the adsorption isotherm.Meanwhile,the release rate,migration coefficient,and bioavailability parameters of selenium are calculated by fitting the first-order kinetic model,further verifying the reliability and applicability of the DGT data.The experimental results demonstrate that under continuous wet conditions,the release rate of soil selenium reaches 1.85µg·cm^(-2)·h^(-1),with a migration coefficient of 0.012 cm^(2)·h^(-1)and a bioavailability parameter of 0.74;under wet-dry alternating conditions,they are 1.42µg·cm^(-2)·h^(-1),0.01 cm^(2)·h^(-1),and 0.68,respectively;under continuous dry conditions,the release rate of soil selenium is the smallest,at 0.88µg·cm^(-2)·h^(-1),with a migration coefficient of 0.004 cm^(-2)·h^(-1)and a bioavailability parameter of 0.5.The results of this experiment reveal the dynamic behavior of soil selenium under different moisture conditions and reflect the high efficiency of DGT technology in dynamic monitoring and quantitative analysis of soil selenium behavior,providing a scientific basis for the optimal management of rhizosphere soil selenium.
基金supported by the National Natural Science Foundation of China Regional Joint Innovation Fund (No.U22A20524)the Heilongjiang Province Natural Science Foundation Key projects (No.ZD2023C002).
文摘Bisphenol A(BPA)is an industrial pollutant that can cause immune impairment.Selenium acts as an antioxidant,as selenium deficiency often accompanies oxidative stress,resulting in organ damage.This study is the first to demonstrate that BPA and/or selenium deficiency induce pyroptosis and ferroptosis-mediated thymic injury in chicken and chicken lymphoma cell(MDCC-MSB-1)via oxidative stress-induced endoplasmic reticulum(ER)stress.We established a broiler chicken model of BPA and/or selenium deficiency exposure and collected thymus samples as research subjects after 42 days.The results demonstrated that BPA or selenium deficiency led to a decrease in antioxidant enzyme activities(T-AOC,CAT,and GSH-Px),accumulation of peroxides(H2O2 and MDA),significant upregulation of ER stress-relatedmarkers(GRP78,IER 1,PERK,EIF-2α,ATF4,and CHOP),a significant increase in iron ion levels,significant upregulation of pyroptosis-related gene(NLRP3,ASC,Caspase1,GSDMD,IL-18 and IL-1β),significantly increase ferroptosis-related genes(TFRC,COX2)and downregulate GPX4,HO-1,FTH,NADPH.In vitro experiments conducted in MDCC-MSB-1 cells confirmed the results,demonstrating that the addition of antioxidant(NAC),ER stress inhibitor(TUDCA)and pyroptosis inhibitor(Vx765)alleviated oxidative stress,endoplasmic reticulum stress,pyroptosis,and ferroptosis.Overall,this study concludes that the combined effects of oxidative stress and ER stress mediate pyroptosis and ferroptosis in chicken thymus induced by BPA exposure and selenium deficiency.
基金the Yangzhou City and Yangzhou University Cooperation Program(No.YZ2023209)the Sele Valley Scholars Basic Research Project(No.2301)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)for support。
文摘Selenium is an essential trace element for human beings and it plays a significant role for the health of human nervous system.The strong antioxidant effect of selenium endows the element with the ability to treat various diseases,including Alzheimer's disease(AD).In the body,selenium exists in the forms of selenoproteins,which could treat AD through various pathways,such as inhibiting peroxidation,inhibiting apoptosis signal pathway,reducing the levels of Aβin neurons and alleviating Tau protein caused by pathological damage.This article aims to comprehensively elaborate on the relationship between selenium and AD.
文摘With the wide application of thrombolytic drugs and the advancement of endovascular therapeutic techniques, the recanalization treatment of acute artery occlusion in ischemic stroke (IS) has made a leap forward, but ischemic brain tissues still face ischemia-reperfusion injury after recanalization. Nowadays, effective neurological protective agents still cannot completely resist the multiple damages of ischemia-reperfusion injury. As an iron-dependent mode of programmed cell death, ferroptosis occupies an important position in ischemia-reperfusion injury. Selenium plays a unique protective role in ischemia-reperfusion injury as an active site element in the center of glutathione peroxidase. Therefore, the study mainly aims to review the protective role of selenium in IS and the related mechanisms, as well as the effect of selenium on the risk factors of IS.
基金financially supported by the foundation of the NMPA Key Laboratory for Quality Evaluation of Medical Protective and Implant Devices,the Shandong Provincial Natural Science Foundation(ZR2021MB096)to Huali Niethe Science and Technology Research Project of Shanghai Songjiang District Science and Technology Committee(No.2023SJKWGG063)+4 种基金the Medical Engineering Cross Research Project of Shanghai Jiaotong University(No.YG2022QN074)to Changping Wangthe National Nature Science Foundation of China(No.32371383)the Shanghai 2023“Science and Technology Innovation Action Plan”Biomedical Science and Technology Support Special Project(No.23S31900100)the Foundation of National Center for Translational Medicine(Shanghai)SHU Branch(No.SUITM-202411)to Siyu Nithe Research Center for the Industries of the Future at Westlake University and the Zhejiang Provincial Natural Science Foundation of China(No.2022XHSJJ003)for support.
文摘The Kirschner wire(K-wire)is widely used in orthopedic external fixation due to its versatility and clinical effectiveness.However,a significant challenge associated with its use is the potential for bacterial migration,subsequent infection,and dislodgement as the wire penetrates the skin and bone.This study introduces a novel bioactive material,selenium/calcium silicate(Se/β-CS),achieved by integrating selenium-an essential trace element in the human body-into bioceramic calcium silicate.This integration was accomplished using a combined chemical co-deposition method and redox reaction.Furthermore,a uniform and controllable Se/β-CS coating was applied to the K-wire's surface using the Langmuir-Blodgett technique.This coating gradually releases active components-Si,Ca,and Se-that effectively eliminate bacterial infections and promote osteointegration.The findings of this study offer promising opportunities for the use of robust and multifunctional coating materials on implantable devices,particularly within the fields of orthopedics,transplantation,and surgery.
基金National Key Research and Development Program of China(No.2022YFC2904900)the National Natural Science Foundation of China(No.U1902221).
文摘The erosion process and kinetics of PbTe particles in a selenium melt were investigated.The results reveal that the limiting step of the reaction is controlled by product layer diffusion and the interfacial chemical reaction at low temperatures(573,583,and 593 K),but the limiting step is controlled by boundary layer diffusion at high temperatures(603 and 613 K).The Se-and Te-atom diffusion in the product layer becomes unbalanced as the product layer thickens,with Kirkendall voids generating in the product layer accelerating PbTe particle erosion.After the PbTe impurities in the selenium melt evolve into PbSe and Te,Te is evenly distributed in the selenium melt owing to the solubility of Se and Te.This study serves to clarify the evolution behavior of PbTe impurities in the selenium melt and the reason that Te often occurs in Se.
基金Project(2022YFC2904900) supported by the National Key Research and Development Program of ChinaProject(U1902221) supported by the National Natural Science Foundation of China。
文摘Selenium distillation slag(SDS)is a high-value-added secondary resource with a high recovery value.This paper aims to investigate the leaching behavior and kinetics of selenium,tellurium,and copper in the SDS acid oxidation leaching process with H_(2)SO_(4) and H_(2)O_(2).The experimental results showed that under the optimum conditions,the contents of selenium,tellurium,and copper in the SDS were reduced from 22.13 wt%,3.58 wt%,and 6.42 wt%to 3.06 wt%,0.27 wt%,and 0.33 wt%,respectively.Correspondingly,the recovery rates are 87.08%,97.15%and 99.7%.The leaching processes of selenium and tellurium were controlled by diffusion and chemical reactions,and the leaching behavior of copper was controlled by chemical reactions.Below 45℃,the activation energies for selenium,tellurium,and copper were found to be 26.47,62.18 and 19.67 kJ/mol,respectively.In addition,the contents of lead,silver and gold in the leaching residue are increased to 46.8 wt%,8.35 wt%and 0.27 wt%,respectively.These substances can be utilized as raw materials for the recovery of these valuable metals.Importantly,the entire process does not generate toxic or harmful waste,making it a green and environmentally friendly method for resource recovery.
基金supported by National Natural Science Foundation of China(No.52403042)China Postdoctoral Science Foundation(No.2023M742472)。
文摘Wound dressings with tissue adhesion,good mechanical,antioxidant and anti-inflammatory performance are urgently needed.In this work,we present a multifunctional selenium nanoparticles(SeNPs)/citric acid/gelatin/hydroxysuccinimide-grafted polyacrylic acid nanocomposite hydrogel adhesive(SCA) specifically designed for wound healing applications.The SCA was prepared via a one-pot processing,where SeNPs synthesized via chemical reduction were incorporated.These SeNPs not only endowed SCA with robust wet adhesion ability,excellent stretchability,and skin-matched elasticity modulus by serving as a physical crosslinker to modulate swelling equilibrium and molecular slippage,but also enhanced the biocompatibility and free radical scavenging capacity of SCA.Furthermore,in vivo evaluation of full-thickness cutaneous defects of rats revealed that SCA effectively reduced inflammation,promoted wound closure,and increased collagen deposition.All these results demonstrated that the developed SCA offers a promising therapeutic strategy for wound healing applications.
基金Supported by the Natural Science Foundation of the Heilongjiang Province of China(C2016003)China Postdoctoral Science Foundation(2015M581415)Heilongjiang Postdoctoral Fund(LBH-Z15005)。
文摘The trace element selenium(Se)occurs naturally throughout the earth.Se deficiency has been linked to impaired breast health and other diseases in human and animals.Compared to severe Se deficiency,marginal dietary Se deficiency accusers more frequently in low-Se regions.Therefore,to investigate the Se status and inflammatory response of the mammary gland under marginal dietary Se levels,an lipopolysaccharide(LPS)induced mouse mastitis model was established.Mice were fed with moderate Se diet(0.087 mg•kg^(-1) Se),adequate Se diet(0.15 mg•kg^(-1) Se)or excessive Se diet(1.5 mg•kg^(-1) Se)for 60 days.Se status and inflammatory factors were investigated.Results showed that the Se status of mammary gland correlated with dietary Se levels.Marginal Se deficiency exacerbated mammary tissue histopathology;increased the mRNA level of inflammatory genes tumor necrosis factor alpha(TNF-α),interleukin-1β(IL-1β)and cyclooxygenase-2(COX-2);and enhanced the phosphorylation of NF-κB p65 in mammary gland tissues.Supplementation of Se in diet higher than recommended levels reduced the inflammatory reaction of mammary glands in LPS-induced mastitis model and provided a protective effect.
基金supported by Key Research and Development Program of Shaanxi(2024NC-GJHX-12)the National Natural Science Foundation of China(32172301).
文摘Selenium nanoparticles(SeNPs)are increasingly recognized for their exceptional antibacterial properties.This study aimed to develop a green,safe,and efficient method for the biosynthesis of SeNPs using the fungus Eurotium cristatum,a novel approach in SeNP synthesis.The process yielded(36.40±4.22)mg of SeNPs per liter of 1.2 mmol/L sodium selenite supplementation.These SeNPs exhibited an average diameter of 231.7 nm and a negative charge,and they remained stable when stored at 4℃.Ultraviolet and visible spectrophotometry revealed a maximum absorption peak at 212 nm,suggesting effective nanoparticle formation.Fourier transform infrared spectrometry indicated that proteins and carbohydrates in the mycelium contributed to the SeNP synthesis.Concentrations of SeNPs below 50μg Se/mL did not exhibit cytotoxic effects on the growth and proliferation of human hepatocyte L-02 cells.The minimum inhibitory concentration of SeNPs was found to be 2 mg/mL against both methicillin-resistant Staphylococcus aureus(Gram-positive)and Escherichia coli(Gram-negative).The SeNPs compromised the cellular integrity of test strains,causing leakage of intracellular contents and disruption of the oxidative stress system,leading to irreversible damage.Our results demonstrate the potential of SeNPs biosynthesized by E.cristatum to act as effective antibacterial agents,signifying a novel and promising approach to developing natural antimicrobial solutions.
基金supported by grants from the National Natural Science Foundation of China(NSFC,81960588)the Ningxia Natural Science Foundation(2020AAC03146)support from the Ningxia Medical University。
文摘Selenium is a crucial trace element that contributes to physiological processes in the body as selenoproteins.Selenoproteins serve as an integral role in the body in controlling the redox state of cells and protecting against damage induced by oxidative stress.This study aimed to investigate the effects and possible mechanism of selenium on selenoproteins expression in EA.hy926 cells induced by oxidized low density lipoprotein(oxLDL).The impact of selenium on the viability of EA.hy926 cells was detected by the methylthiazolyldiphenyltetrazolium bromide(MTT)method,and intracellular reactive oxygen species(ROS)level and mitochondrial membrane potential were assessed by fluorescent probe DCFH-DA and JC-1,respectively.RNA-seq,quantitative real-time polymerase chain reaction(qPCR),and Western blot were used to investigate the selenoprotein expression.Selenoprotein mRNA translation efficiency was analyzed by ribosome profiling(Ribo-Seq)coupled with transcriptomics.Our data showed that selenium supplementation(0.5μmol/L)significantly decreased ROS production,increased mitochondrial inner membrane potential and increased the proliferative activity of EA.hy926 cells induced by oxLDL.Moreover,The protective effects of selenium against oxLDL-induced EA.hy926 cell injury were associated with the upregulation of the expressions of selenoproteins glutathione peroxidase 1(GPX1),glutathione peroxidase 4(GPX4),and thioredoxin reductase 1(TXNRD1).Furthermore,the expressions of selenoproteins GPX1 and GPX4 were hierarchically controlled,but the expressions of selenoproteins TXNRD1 were mainly regulated by oxLDL.Finally,Ribo-Seq coupled with transcriptomics results demonstrated that the expressions of selenoproteins GPX1,GPX4,and TXNRD1 were regulated at the translation process level.These findings suggested that selenium could have preventive effects in oxLDL induced EA.hy926 cell injury by regulating the selenoprotein expression,and the selenoproteins expressions at the translation level in vascular endothelial cells need further study.
