Krill oil is effective in reducing blood lipid levels,particularly in individuals with severe hyperlipidemia.However,poor water insolubility and stability limited its usage.This study investigated a method for encapsu...Krill oil is effective in reducing blood lipid levels,particularly in individuals with severe hyperlipidemia.However,poor water insolubility and stability limited its usage.This study investigated a method for encapsulating Antarctic krill oil using alginate(ALG)and gelatin(GLN)to enhance its stability and bioactivity.The encapsulation efficiency,functional group integrity,swelling rate,and lipid-lowering activity were assessed.Results indicated that the optimal encapsulation conditions were identified with an ALG:GLN ratio of 2:1(m/m),coagulation bath of 9%CaCl_(2),and a nozzle size of 750μm,resulting in 69.34%encapsulation efficiency.Fourier-transform infrared spectroscopy confirmed successful encapsulation.The ALG-GLN shell materials enriched astaxanthin in krill oil and protected it from harsh gastric conditions,enabling targeted intestinal release.In a high-fat diet-induced rat model,krill oil microcapsules significantly reduced triglycerides(TG),total cholesterol(TC),and low-density lipoprotein-cholesterol(LDL-C)levels while increasing high-density lipoprotein-cholesterol(HDL-C)levels compared to unencapsulated krill oil.Additionally,the microcapsules elevated nitric oxide(NO)levels,enhanced superoxide dismutase(SOD)activity,and reduced malondialdehyde(MDA)levels,liver and perirenal fat weight.Therefore,encapsulating Antarctic krill oil in alginate-gelatin hydrogel offers a promising strategy for managing hyperlipidemia and associated metabolic disorders.展开更多
Step ladder-structured nitrocellulose(LNC)is a novel energetic binder prepared by chemically modifying nitrocellulose(NC)with the introduction of flexible polyethylene glycol(PEG-400)chain segments,with a regular stru...Step ladder-structured nitrocellulose(LNC)is a novel energetic binder prepared by chemically modifying nitrocellulose(NC)with the introduction of flexible polyethylene glycol(PEG-400)chain segments,with a regular structure and good performance of bonding.The step ladder-structured addresses critical limitations of NC-based propellants,including low-temperature brittleness and high sensitivity,while enhancing process safety.Although the structural,thermal,and other properties of LNC have been investigated in our previous research,there is a lack of systematic studies on the rheological properties during solution and gelatinization.The study of the relationship between the structural features and rheological properties of LNC is a key factor in guiding its gelatinization and improving the properties of LNC-based propellants.Steady-state rheology flow experiments revealed that LNC exhibited shear thinning in different solutions,which decreased with increasing concentration.It has desirable solu-bility and dispersion in N,N-dimethylformamide(DMF)solvent.The effect of solvents on the entan-glement or orientation of LNC molecular chains may be reduced.These results can be quantitatively demonstrated using the Herschel-Bulkley model.Dynamic viscoelastic studies identified a critical point of concentration-frequency of 2.5 rad/s.This particular frequency point is a turning point in the law of the effect of concentration on the loss factor(tanδ).For gelatinized systems,increasing the solvent content reduces the temperature sensitivity of the gelatinized materials.The viscosity-temperature correlation based on the Arrhenius equation allowed the optimization of the solvent content through the derived equilibrium relationship.These structure-rheological performance relationships establish basic guidelines for the precision gelatinization of LNC-based propellant,provide theoretical support for the replacement of conventional NC by LNC,and guide the gelatinization process to improve the performance of gun propellants.展开更多
Corresponding author’s name was incorrectly written as“Dadang Guo”instead of“Dagang Guo”.The correct author name should be“Dagang Guo”.The authors would like to apologise for any inconvenience caused.
Oral ulcers may greatly diminish patient life quality and potentially result in malignant transformations.Using gels or films as pseudomembrane barriers is an effective method for promoting ulcer healing.However,these...Oral ulcers may greatly diminish patient life quality and potentially result in malignant transformations.Using gels or films as pseudomembrane barriers is an effective method for promoting ulcer healing.However,these pseudomembranes face challenges such as saliva flushing,dynamic changes,and the presence of abundant microorganisms in the complex oral environment.Herein,we developed an injectable,photoinduction,in situ-enhanceable oral ulcer repair hydrogel(named as GIL2)by incorporating dynamic phenylboronic acid ester bonds and imidazole ions into a methacrylated gelatin matrix.GIL2 exhibited rapid gelation(3 s),low swelling properties(1.07 g/g),robust tensile strength(56.83 kPa)and high adhesive strength(63.38 kPa),allowing it to adhere effectively to the ulcer surface.Moreover,the GIL2 demonstrated intrinsic antibacterial and antioxidant qualities.Within a diabetic rat model for oral ulcers,GIL2 effectively eased oxidative stress and decreased the inflammation present in ulcerated wounds,thereby greatly hastening the healing process of these ulcers.Together,GIL2 hydrogel demonstrates remarkable adaptability within the oral milieu,revitalizing clinical strategy advancements for treating bacterialinfected oral ulcers.展开更多
Delaying skin-aging through diet is a hot research topic in recent years,but the anti-aging effects of fish gelatin and related mechanisms are not well understood.In this study,we prepared edible fish gelatin from the...Delaying skin-aging through diet is a hot research topic in recent years,but the anti-aging effects of fish gelatin and related mechanisms are not well understood.In this study,we prepared edible fish gelatin from the swim bladder of Cynoscion acoupa using three different processing methods,namely dried(DCM),soaked(SCM)and instanted(ICM),to investigate its anti-aging effects and mechanisms on D-galactose induced skin aging in mice,as well as its effects on the gut microbiota.The results demonstrated that fish gelatin significantly increased water content,collagen,hyaluronic acid(HA)and hydroxyproline(Hyp)content,and skin integrity of mice skin,as well as enhanced the antioxidative ability and anti-inflammatory capacity of the skin.In terms of protein and mRNA expression levels in skin tissue,CMs treated with different treatments can up-regulate the expression of epidermal growth factor receptor(EGFR)and tissue inhibitor of metal protease 1(TIMP1),down-regulating the expression of matrix metalloproteinase 1(MMP1)and matrix metalloproteinase 3(MMP3),and increase the expression of collagen type III alpha 1 chain(COL3A1)and collagen type I alpha 2 chain(COL1A2).CMs attenuated the D-galactose-mediated inhibition collagen expression by stimulating the transforming growth factor beta(TGF-β)/Smad signaling pathway,thereby maintaining collagen matrix homeostasis.In addition,we revealed that CMs reversed gut microbiota by increase the abundance of intestinal flora.In conclusion,we demonstrated that CMs,especially for ICM,as an effective dietary supplement,have potential anti-aging and skin health benefits.展开更多
Conductive hydrogels derived from natural polymers have attracted increasing attention in wearable electronics due to their inherent biocompatibility and sustainability.However,their poor mechanical strength,limited c...Conductive hydrogels derived from natural polymers have attracted increasing attention in wearable electronics due to their inherent biocompatibility and sustainability.However,their poor mechanical strength,limited conductivity and unsatisfactory environmental adaptability remain significant challenges fo r practical applications.In this study,we report a high-performance gelatin-based conductive hydrogel(GPC)reinforced with polypyrrole-decorated cellulose nanofibers(PPy@CNF)and enhanced by a zwitterionic betaine/(NH_(4))_(2)SO_(4) solution.The PPy@CNF hybrid nanofillers were synthesized via in situ oxidative polymerization,enabling homogeneous dispersion of PPy along the CNF su rface.The incorporation of PPy@CNF significantly improved both mechanical strength and conductivity of the gelatin hydrogel.Meanwhile,the Hofmeister effect induced by(NH_(4))_(2)SO_(4) strengthened the hydrogel network,and the introduction of betaine further enhanced its anti-freezing and moisture-retention properties.The optimized GPC hydrogel exhibited a high tensile strength of 1.02 MPa,conductivity of 1.5 S·m^(-1),and stable performance at temperatures down to-50℃.Furthermore,it was successfully assembled into a wearable strain sensor for real-time human motion monitoring,and as an electrode layer in a flexible triboelectric nanogenerator(TENG),enabling biomechanical energy harvesting and self-powered sensing.This work provides a promising strategy for developing sustainable,multifu nctional hydrogels for next-generation weara ble electronics.展开更多
The notorious shuttle effect of polyiodides in aqueous Zinc-iodine(Zn-I2)batteries impedes their practical application,which renders it imperative to address this issue.Here,we report natural gelatin as an advanced aq...The notorious shuttle effect of polyiodides in aqueous Zinc-iodine(Zn-I2)batteries impedes their practical application,which renders it imperative to address this issue.Here,we report natural gelatin as an advanced aqueous binder for iodine-loading cathode to enable stable and efficient Zn-I_(2) batteries.The positively charged region in gelatin presents electrostatic attraction to the iodine species,while the electron-rich regions could donate electrons to form physical or even covalent bonds with iodine species,thus inhibiting polyiodides shuttle effect and boosting redox reaction.A high reversible capacity of 138 mAh g^(-1) after 3000 cycles at 2C and an ultra-long cycling stability of 30000 cycles at 25C with 107 mAh g^(-1) capacity was achieved.Gelatin binder also can accommodate high iodine-loading(~10 mg)cathode,punch cells,and severe temperature conditions(-10℃ and 60℃).In-situ UV-vis absorption spectroscopy,in-situ Raman spectra and theoretical calculation revealed the critical role of gelatin binder in suppressing polyiodide shuttling and accelerating reaction kinetics.This work uncovers the potential of natural low-cost binder material in advanced Zn-I_(2) batteries and drives future study of designing functional binders.展开更多
Fish gelatin films have emerged as eco-friendly packaging materials due to their biodegradability and excellent film-forming properties.This study investigated the effects of varying homogenization rates(0,6500,9500,1...Fish gelatin films have emerged as eco-friendly packaging materials due to their biodegradability and excellent film-forming properties.This study investigated the effects of varying homogenization rates(0,6500,9500,13,500,17,500,and 21,500 rpm)on the functional and structural properties of fish gelatin films enriched with cinnamon essential oil(CEO).Homogenization reduced droplet sizes and narrowed droplet size distributions in the film-forming emulsion(FFE).At a homogenization rate of 9500 rpm,the films exhibited excellent mechan-ical extensibility,reduced surface irregularities,and enhanced smoothness.The highest(p<0.05)tensile strength and elongation at break were observed at this rate,showing increases of 57%and 14%,respectively,compared to the control film made from non-homogenized FFE.However,further increases in homogenization rates signifi-cantly increased(p<0.05)water vapor permeability and caused CEO droplets aggregation,leading to increased surface irregularities and the formation of pores in the film microstructure.These structural changes were observed through confocal laser scanning microscopy(CLSM),scanning electron microscopy(SEM),and atomic force microscopy(AFM).These findings indicate that the film properties were significantly influenced by the homogenization rate,highlighting its role in tailoring the mechanical and barrier properties of fish gelatin/CEO films for food packaging applications.展开更多
The uncontrollable dendrite growth of lithium anode and active material dissolution of transition metal oxides cathodes severely hinder the development of lithium metal batteries.An effective strategy to address these...The uncontrollable dendrite growth of lithium anode and active material dissolution of transition metal oxides cathodes severely hinder the development of lithium metal batteries.An effective strategy to address these issues is optimizing the separator to regulate ion transport and trap the lost active component.Herein,a crosslinked gelatin nonwoven(CGN)separator is elaborately fabricated through electrospinning and in-situ vapor phase crosslinking process to manipulate the dual electrode interface.Benefitting from the characteristic composition of gelatin,and porous structure of electrospun nonwoven,the CGN separator exhibits excellent interface wettability and low interface resistance,featuring a high Li^(+)transference number of 0.70 and high ionic conductivity of 3.75 m S/cm.As expected,the symmetrical Li/Li cells present stable cycling behavior for 1900 h at 0.5 mA/cm^(2)with low overpotential of 20 mV.The optimized LiMn_(2)O_(4)/Li cells deliver high reversible capacity of 103 m Ah/g as well as high capacityretention ratio of 83.7%after 100 cycles at 0.3 C,which can be effectively attributed to the strong interaction between CGN separator and Mn ions to prevent the loss of active Mn component.This study indicates the application potential of protein-based electrospun membrane for high-performance lithium metal batteries.展开更多
The preparation and properties of adriamycin magnetic gelatin microspheres(Adr- MG-ms)were reported.The synthesis of magnetic iron oxide ultrafine particle and embolization effects of magnetic gelatin microspheres(MG-...The preparation and properties of adriamycin magnetic gelatin microspheres(Adr- MG-ms)were reported.The synthesis of magnetic iron oxide ultrafine particle and embolization effects of magnetic gelatin microspheres(MG-ms)in dog were studied.Adr- MG-ms consist of 2%(w/w)of adriamycin(Adr)as the core,and 68% of gelatin and 30% of magnetite as the shell with a mean particle size of 22 μm. In vitro experiment,the release rate of drug demonstrated that the microspheres have sustained-release properties.The average diameter of magnetic iron oxide was approximately l0 nm. Transcatheter embolization with MG-ms and ￣(99m)Tc-labelled MG-ms was performed under external magenet control in dog liver,respectively.Gamma photography and angiogram revealed that MG-ms level was almost equal both in left and right hepatic arteries without magnet,while with magnet(1200 Gs),MG-ms level in left hepatic artery(target site)was about 2.25 fold higher than in right hepatic artery,and few MG-ms in thyroid gland,brain and heart was observed.Results showed that the MG-ms is a promising embolic agent for treatment of hepatic cancer under external magnet control.展开更多
Objective] This study aimed to investigate the method for efficient utilization and development of purple sweet potatoes. [Method] Purple sweet potatoes were dried at two specific temperatures and prepared into prelim...Objective] This study aimed to investigate the method for efficient utilization and development of purple sweet potatoes. [Method] Purple sweet potatoes were dried at two specific temperatures and prepared into preliminarily-processed products for gelatinization simulation to analyze the extraction amount of anthocyanins from gelatinized samples at different gelatinization stages. [Result] During the gelatinization process, the extraction rate of anthocyanins from purple sweet potato samples reached the highest as the temperature rised from 90 ℃ to 95 ℃,and the extraction amount of anthocyanins reached the maximum at 15 min postheat preservation at 95 ℃. Purple sweet potato samples dried at 60 ℃ exhibited larger retention amount, larger maximum extraction amount and higher maximum extraction rate of anthocyanins compared with those dried at 110 ℃. [Conclusion] Drying at low temperatures and appropriately shortening the initial gelatinization stage below 90 ℃ is conducive to the retention and extraction of anthocyanins from purple sweet potatoes.展开更多
The enzymatic hydrolysis of macromolecular gelatin with AS1.398 neutral protease, bromelain and their combinations was studied by estimating the molecular weights of their hydrolytic products. It was discovered that t...The enzymatic hydrolysis of macromolecular gelatin with AS1.398 neutral protease, bromelain and their combinations was studied by estimating the molecular weights of their hydrolytic products. It was discovered that the products hydrolyzed by using combination enzymes had lower molecular weight than those obtained by using single ones, and the kind of enzymes, their combination mode and addition sequence are effective ways to control the molecular weights of gelatin hydrolyzates.展开更多
Porous titanium with porosity of 60% was prepared by metal injection molding（MIM）,and coated with gelatin sustained-release microspheres which were made by improved emulsified cold condensation method.The effects of...Porous titanium with porosity of 60% was prepared by metal injection molding（MIM）,and coated with gelatin sustained-release microspheres which were made by improved emulsified cold condensation method.The effects of porous titanium coated with insulin-like growth factor-1（IGF-1） and transforming growth factor-β1（TGF-β1） gelatin microspheres on the function of MG63 cells were evaluated in vitro.The results show that porous titanium coated with gelatin sustained-release microspheres has no cytotoxicity.The IGF-1 and TGF-β1 loading concentrations are positively correlative with the proliferation and differentiation of MG63 after co-culturing with the concentrations of IGF-1 and TGF-β1 gelatin microspheres in the range of 0.1-10 ng/mg and 0.25-2.5 ng/mg,respectively.The MG63 cells exhibit the best proliferation and differentiation with the IGF-1 and TGF-β1 loading concentrations of 10 ng/mg and 2.5 ng/mg,respectively.The joint application of IGF-1 and TGF-β1 group,which promote adhesion,proliferation and differentiation of MG63 cells,is superior to a single application group.展开更多
Polyvinyl alcohol gelatin hydrogels were fabricated using genipin as a crosslinking agent for bone regeneration application. Optimized formulation of PVA-GE hydrogel was fabricated using genipin as crosslinking agent....Polyvinyl alcohol gelatin hydrogels were fabricated using genipin as a crosslinking agent for bone regeneration application. Optimized formulation of PVA-GE hydrogel was fabricated using genipin as crosslinking agent. Characterizations such as FTIR, morphology, porosity, pore size, degradation and swelling rate were investigated. Bone regeneration potential of optimized genipin cross-linked polyvinyl alcohol-gelatin (PVA20) hydrogels was assessed by implanting in rabbit’s femur defect for 1, 5 and 15 weeks period. Results showed interconnected porosity as observed in scanning electron microscopy and successful crosslinking as confirmed by FTIR analysis. Increased porosity (92% ± 2.46%) and pore size distribution (100 - 200 μm) were also observed as well as decrease in swelling rate (426% ± 10.50%). Bone formation was evident in micro-CT after 5 and 15 days of in vivo implantation period. Micro-CT analysis showed 32.67% increased bone formation of PVA-GE hydrogel defect compared with negative control after 15 weeks of in-vivo implantation. Histological analyses showed no inflammatory reaction post implantation and increase in cell matrix formation after 5 and 15 weeks. The combined physical and chemical method of crosslinking promises improved mechanical properties of PVA-GE hydrogel making it a potential scaffold for bone tissue engineering applications.展开更多
To achieve effective decolorization of reactive dyes,laccase immobilization was investigated.Laccase 0.2%(m/V)(Denilite IIS) was trapped in beads of alginate/gelatin blent with polyethylene glycol(PEG),and then the su...To achieve effective decolorization of reactive dyes,laccase immobilization was investigated.Laccase 0.2%(m/V)(Denilite IIS) was trapped in beads of alginate/gelatin blent with polyethylene glycol(PEG),and then the supporters were activated by cross-linking with glutaraldehyde.The results of repeated batch decolorization showed that gelatin and appropriate concentration of glutaraldehyde accelerated the decolorization of Reactive Red B-3BF(RRB);PEG had a positive effect on enzyme stability and led to an inc...展开更多
Paddy rice samples were parboiled by soaking at 65℃ for 180 min and steaming at 96℃ for 2–10 min,and then dried to achieve the final moisture content of 11% ± 1%. The degree of starch gelatinization （DSG） an...Paddy rice samples were parboiled by soaking at 65℃ for 180 min and steaming at 96℃ for 2–10 min,and then dried to achieve the final moisture content of 11% ± 1%. The degree of starch gelatinization （DSG） andseveral quality attributes （head rice yield （HRY）, color value and hardness） of parboiled rice were measured.Results showed that DSG （46.8%–77.9%）, color value （18.08–19.04） and hardness （118.6–219.2 N） allincreased following steaming. In contrast, the HRY increased （64.8%–67.1%） for steaming times between 2–4min but decreased （67.1%–65.0%） for steaming times between 4–10 min. Linear relations between DSG andcolor value （R2 = 0.87）, and DSG and hardness （R2 = 0.88） were observed. The suitable DSG of parboiled riceleading to the highest HRY was found to be 62.5%, obtained following 4 min of steaming.展开更多
Gelatin/Alginate hydrogels were engineered for bioplotting in tissue engineering.One major drawback of hydrogel scaffolds is the lack of adequate mechanical properties.In this study,using a bioplotter,we constructed t...Gelatin/Alginate hydrogels were engineered for bioplotting in tissue engineering.One major drawback of hydrogel scaffolds is the lack of adequate mechanical properties.In this study,using a bioplotter,we constructed the scaffolds with different pore architectures by deposition of gelatin/alginate hydrogels layerby-layer.The scaffolds with different crosslinking degree were obtained by post-crosslinking methods.Their physicochemical properties,as well as cell viability,were assessed.Different crosslinking methods had little influence on scaffold architecture,porosity,pore size and distribution.By contrast,the water absorption ability,degradation rate and mechanical properties of the scaffolds were dramatically affected by treatment with various concentrations of crosslinking agent(glutaraldehyde).The crosslinking process using glutaraldehyde markedly improved the stability and mechanical strength of the hydrogel scaf-folds.Besides the post-processing methods,the pore architecture can also evidently affect the mechanical properties of the scaffolds.The crosslinked gelatin/alginate scaffolds showed a good potential to encap-sulate cells or drugs.展开更多
The objective of this research was to develop gelatin microspheres（GMSs） and study their properties for embolization.The GMSs were prepared by emulsion chemical crosslinking method.The morphology and particle size o...The objective of this research was to develop gelatin microspheres（GMSs） and study their properties for embolization.The GMSs were prepared by emulsion chemical crosslinking method.The morphology and particle size of GMSs were observed under an optical microscope.The ratio of water absorption and GMSs swelling ratio were measured.The elasticity of GMSs was studied by TMS-Pro food texture analyzer.The deliverability of GMSs through catheter was investigated with a new device designed in this study.Finally,the acetone residue was determined by headspace capillary gas chromatography.The dried GMSs were elliptic with corrugated surface and the wet GMSs were round with smooth surface.The average diameter of dried GMSs was 430.5 μm with a range of 100-1000 μm,and that of wet GMSs was 601.2 μm with a range of 150-1425 μm.The maximum ratio of water absorption was 590.7% and the average swelling ratio was 103.0%.The elasticity of GMSs was proven to be appropriate for embolization.Three subgroups of wet GMSs（100-450 μm,450-700 μm and 700-940 μm） were delivered through catheter with acceptable pressure.The content of residual acetone in GMSs was less than the minimum limit in the Chinese Pharmacopoeia 2010.Therefore,we concluded that the GMSs were suitable for embolization according to the applicable properties in vitro.And the methods for assessing the properties of GMSs in this study were going to be useful in the evaluation of embolic microspheres.展开更多
基金funded by the National Key Research and Development Program of China(2023YFF1103804)the Academic Research Projects of Beijing Union University(ZK20202516).
文摘Krill oil is effective in reducing blood lipid levels,particularly in individuals with severe hyperlipidemia.However,poor water insolubility and stability limited its usage.This study investigated a method for encapsulating Antarctic krill oil using alginate(ALG)and gelatin(GLN)to enhance its stability and bioactivity.The encapsulation efficiency,functional group integrity,swelling rate,and lipid-lowering activity were assessed.Results indicated that the optimal encapsulation conditions were identified with an ALG:GLN ratio of 2:1(m/m),coagulation bath of 9%CaCl_(2),and a nozzle size of 750μm,resulting in 69.34%encapsulation efficiency.Fourier-transform infrared spectroscopy confirmed successful encapsulation.The ALG-GLN shell materials enriched astaxanthin in krill oil and protected it from harsh gastric conditions,enabling targeted intestinal release.In a high-fat diet-induced rat model,krill oil microcapsules significantly reduced triglycerides(TG),total cholesterol(TC),and low-density lipoprotein-cholesterol(LDL-C)levels while increasing high-density lipoprotein-cholesterol(HDL-C)levels compared to unencapsulated krill oil.Additionally,the microcapsules elevated nitric oxide(NO)levels,enhanced superoxide dismutase(SOD)activity,and reduced malondialdehyde(MDA)levels,liver and perirenal fat weight.Therefore,encapsulating Antarctic krill oil in alginate-gelatin hydrogel offers a promising strategy for managing hyperlipidemia and associated metabolic disorders.
基金supported by the National Natural Science Foundation of China(No.22475100 and 22075146).
文摘Step ladder-structured nitrocellulose(LNC)is a novel energetic binder prepared by chemically modifying nitrocellulose(NC)with the introduction of flexible polyethylene glycol(PEG-400)chain segments,with a regular structure and good performance of bonding.The step ladder-structured addresses critical limitations of NC-based propellants,including low-temperature brittleness and high sensitivity,while enhancing process safety.Although the structural,thermal,and other properties of LNC have been investigated in our previous research,there is a lack of systematic studies on the rheological properties during solution and gelatinization.The study of the relationship between the structural features and rheological properties of LNC is a key factor in guiding its gelatinization and improving the properties of LNC-based propellants.Steady-state rheology flow experiments revealed that LNC exhibited shear thinning in different solutions,which decreased with increasing concentration.It has desirable solu-bility and dispersion in N,N-dimethylformamide(DMF)solvent.The effect of solvents on the entan-glement or orientation of LNC molecular chains may be reduced.These results can be quantitatively demonstrated using the Herschel-Bulkley model.Dynamic viscoelastic studies identified a critical point of concentration-frequency of 2.5 rad/s.This particular frequency point is a turning point in the law of the effect of concentration on the loss factor(tanδ).For gelatinized systems,increasing the solvent content reduces the temperature sensitivity of the gelatinized materials.The viscosity-temperature correlation based on the Arrhenius equation allowed the optimization of the solvent content through the derived equilibrium relationship.These structure-rheological performance relationships establish basic guidelines for the precision gelatinization of LNC-based propellant,provide theoretical support for the replacement of conventional NC by LNC,and guide the gelatinization process to improve the performance of gun propellants.
文摘Corresponding author’s name was incorrectly written as“Dadang Guo”instead of“Dagang Guo”.The correct author name should be“Dagang Guo”.The authors would like to apologise for any inconvenience caused.
基金funding from the National Natural Science Foundation of China(Nos.82071170 and 82371016)the Zhejiang Provincial Science and Technology Project for Public Welfare(No.LGF21H140004).
文摘Oral ulcers may greatly diminish patient life quality and potentially result in malignant transformations.Using gels or films as pseudomembrane barriers is an effective method for promoting ulcer healing.However,these pseudomembranes face challenges such as saliva flushing,dynamic changes,and the presence of abundant microorganisms in the complex oral environment.Herein,we developed an injectable,photoinduction,in situ-enhanceable oral ulcer repair hydrogel(named as GIL2)by incorporating dynamic phenylboronic acid ester bonds and imidazole ions into a methacrylated gelatin matrix.GIL2 exhibited rapid gelation(3 s),low swelling properties(1.07 g/g),robust tensile strength(56.83 kPa)and high adhesive strength(63.38 kPa),allowing it to adhere effectively to the ulcer surface.Moreover,the GIL2 demonstrated intrinsic antibacterial and antioxidant qualities.Within a diabetic rat model for oral ulcers,GIL2 effectively eased oxidative stress and decreased the inflammation present in ulcerated wounds,thereby greatly hastening the healing process of these ulcers.Together,GIL2 hydrogel demonstrates remarkable adaptability within the oral milieu,revitalizing clinical strategy advancements for treating bacterialinfected oral ulcers.
基金supported by the China Agriculture Research System of MOF and MARA(CARS-21)Key-Area Research and Development Program of Guangdong Province(2022B0202050001).
文摘Delaying skin-aging through diet is a hot research topic in recent years,but the anti-aging effects of fish gelatin and related mechanisms are not well understood.In this study,we prepared edible fish gelatin from the swim bladder of Cynoscion acoupa using three different processing methods,namely dried(DCM),soaked(SCM)and instanted(ICM),to investigate its anti-aging effects and mechanisms on D-galactose induced skin aging in mice,as well as its effects on the gut microbiota.The results demonstrated that fish gelatin significantly increased water content,collagen,hyaluronic acid(HA)and hydroxyproline(Hyp)content,and skin integrity of mice skin,as well as enhanced the antioxidative ability and anti-inflammatory capacity of the skin.In terms of protein and mRNA expression levels in skin tissue,CMs treated with different treatments can up-regulate the expression of epidermal growth factor receptor(EGFR)and tissue inhibitor of metal protease 1(TIMP1),down-regulating the expression of matrix metalloproteinase 1(MMP1)and matrix metalloproteinase 3(MMP3),and increase the expression of collagen type III alpha 1 chain(COL3A1)and collagen type I alpha 2 chain(COL1A2).CMs attenuated the D-galactose-mediated inhibition collagen expression by stimulating the transforming growth factor beta(TGF-β)/Smad signaling pathway,thereby maintaining collagen matrix homeostasis.In addition,we revealed that CMs reversed gut microbiota by increase the abundance of intestinal flora.In conclusion,we demonstrated that CMs,especially for ICM,as an effective dietary supplement,have potential anti-aging and skin health benefits.
基金financially supported by the PhD research startup foundation of China West Normal University(No.22kE038)。
文摘Conductive hydrogels derived from natural polymers have attracted increasing attention in wearable electronics due to their inherent biocompatibility and sustainability.However,their poor mechanical strength,limited conductivity and unsatisfactory environmental adaptability remain significant challenges fo r practical applications.In this study,we report a high-performance gelatin-based conductive hydrogel(GPC)reinforced with polypyrrole-decorated cellulose nanofibers(PPy@CNF)and enhanced by a zwitterionic betaine/(NH_(4))_(2)SO_(4) solution.The PPy@CNF hybrid nanofillers were synthesized via in situ oxidative polymerization,enabling homogeneous dispersion of PPy along the CNF su rface.The incorporation of PPy@CNF significantly improved both mechanical strength and conductivity of the gelatin hydrogel.Meanwhile,the Hofmeister effect induced by(NH_(4))_(2)SO_(4) strengthened the hydrogel network,and the introduction of betaine further enhanced its anti-freezing and moisture-retention properties.The optimized GPC hydrogel exhibited a high tensile strength of 1.02 MPa,conductivity of 1.5 S·m^(-1),and stable performance at temperatures down to-50℃.Furthermore,it was successfully assembled into a wearable strain sensor for real-time human motion monitoring,and as an electrode layer in a flexible triboelectric nanogenerator(TENG),enabling biomechanical energy harvesting and self-powered sensing.This work provides a promising strategy for developing sustainable,multifu nctional hydrogels for next-generation weara ble electronics.
基金supported by National Natural Science Foundation of China(22309029,52404316)Guangdong Basic and Applied Basic Research Foundation(Nos.2024A1515140011,and 2024A1515110010)+2 种基金Dongguan Social Development Technology Foundation(Nos.20231800907933,and 20221800905122)Collaborative Innovation Center of Marine Science and Technology of Hainan University(No.XTCX2022HYC14)Start-up Research Foundation of Hainan University(No.KYQD(ZR)-23069).
文摘The notorious shuttle effect of polyiodides in aqueous Zinc-iodine(Zn-I2)batteries impedes their practical application,which renders it imperative to address this issue.Here,we report natural gelatin as an advanced aqueous binder for iodine-loading cathode to enable stable and efficient Zn-I_(2) batteries.The positively charged region in gelatin presents electrostatic attraction to the iodine species,while the electron-rich regions could donate electrons to form physical or even covalent bonds with iodine species,thus inhibiting polyiodides shuttle effect and boosting redox reaction.A high reversible capacity of 138 mAh g^(-1) after 3000 cycles at 2C and an ultra-long cycling stability of 30000 cycles at 25C with 107 mAh g^(-1) capacity was achieved.Gelatin binder also can accommodate high iodine-loading(~10 mg)cathode,punch cells,and severe temperature conditions(-10℃ and 60℃).In-situ UV-vis absorption spectroscopy,in-situ Raman spectra and theoretical calculation revealed the critical role of gelatin binder in suppressing polyiodide shuttling and accelerating reaction kinetics.This work uncovers the potential of natural low-cost binder material in advanced Zn-I_(2) batteries and drives future study of designing functional binders.
文摘Fish gelatin films have emerged as eco-friendly packaging materials due to their biodegradability and excellent film-forming properties.This study investigated the effects of varying homogenization rates(0,6500,9500,13,500,17,500,and 21,500 rpm)on the functional and structural properties of fish gelatin films enriched with cinnamon essential oil(CEO).Homogenization reduced droplet sizes and narrowed droplet size distributions in the film-forming emulsion(FFE).At a homogenization rate of 9500 rpm,the films exhibited excellent mechan-ical extensibility,reduced surface irregularities,and enhanced smoothness.The highest(p<0.05)tensile strength and elongation at break were observed at this rate,showing increases of 57%and 14%,respectively,compared to the control film made from non-homogenized FFE.However,further increases in homogenization rates signifi-cantly increased(p<0.05)water vapor permeability and caused CEO droplets aggregation,leading to increased surface irregularities and the formation of pores in the film microstructure.These structural changes were observed through confocal laser scanning microscopy(CLSM),scanning electron microscopy(SEM),and atomic force microscopy(AFM).These findings indicate that the film properties were significantly influenced by the homogenization rate,highlighting its role in tailoring the mechanical and barrier properties of fish gelatin/CEO films for food packaging applications.
基金supported by National Natural Science Foundation of China(No.22309029)Guangdong Basic and Applied Basic Research Foundation(No.2024A1515140011)+1 种基金Dongguan Social Development Technology Foundation(No.20231800907933)Collaborative Innovation Center of Marine Science and Technology of Hainan University(No.XTCX2022HYC14)。
文摘The uncontrollable dendrite growth of lithium anode and active material dissolution of transition metal oxides cathodes severely hinder the development of lithium metal batteries.An effective strategy to address these issues is optimizing the separator to regulate ion transport and trap the lost active component.Herein,a crosslinked gelatin nonwoven(CGN)separator is elaborately fabricated through electrospinning and in-situ vapor phase crosslinking process to manipulate the dual electrode interface.Benefitting from the characteristic composition of gelatin,and porous structure of electrospun nonwoven,the CGN separator exhibits excellent interface wettability and low interface resistance,featuring a high Li^(+)transference number of 0.70 and high ionic conductivity of 3.75 m S/cm.As expected,the symmetrical Li/Li cells present stable cycling behavior for 1900 h at 0.5 mA/cm^(2)with low overpotential of 20 mV.The optimized LiMn_(2)O_(4)/Li cells deliver high reversible capacity of 103 m Ah/g as well as high capacityretention ratio of 83.7%after 100 cycles at 0.3 C,which can be effectively attributed to the strong interaction between CGN separator and Mn ions to prevent the loss of active Mn component.This study indicates the application potential of protein-based electrospun membrane for high-performance lithium metal batteries.
文摘The preparation and properties of adriamycin magnetic gelatin microspheres(Adr- MG-ms)were reported.The synthesis of magnetic iron oxide ultrafine particle and embolization effects of magnetic gelatin microspheres(MG-ms)in dog were studied.Adr- MG-ms consist of 2%(w/w)of adriamycin(Adr)as the core,and 68% of gelatin and 30% of magnetite as the shell with a mean particle size of 22 μm. In vitro experiment,the release rate of drug demonstrated that the microspheres have sustained-release properties.The average diameter of magnetic iron oxide was approximately l0 nm. Transcatheter embolization with MG-ms and ￣(99m)Tc-labelled MG-ms was performed under external magenet control in dog liver,respectively.Gamma photography and angiogram revealed that MG-ms level was almost equal both in left and right hepatic arteries without magnet,while with magnet(1200 Gs),MG-ms level in left hepatic artery(target site)was about 2.25 fold higher than in right hepatic artery,and few MG-ms in thyroid gland,brain and heart was observed.Results showed that the MG-ms is a promising embolic agent for treatment of hepatic cancer under external magnet control.
基金Supported by Key Scientific and Technological Project of Wuhan Science and Technology Bureau([2012]No.100 201250499145-15)~~
文摘Objective] This study aimed to investigate the method for efficient utilization and development of purple sweet potatoes. [Method] Purple sweet potatoes were dried at two specific temperatures and prepared into preliminarily-processed products for gelatinization simulation to analyze the extraction amount of anthocyanins from gelatinized samples at different gelatinization stages. [Result] During the gelatinization process, the extraction rate of anthocyanins from purple sweet potato samples reached the highest as the temperature rised from 90 ℃ to 95 ℃,and the extraction amount of anthocyanins reached the maximum at 15 min postheat preservation at 95 ℃. Purple sweet potato samples dried at 60 ℃ exhibited larger retention amount, larger maximum extraction amount and higher maximum extraction rate of anthocyanins compared with those dried at 110 ℃. [Conclusion] Drying at low temperatures and appropriately shortening the initial gelatinization stage below 90 ℃ is conducive to the retention and extraction of anthocyanins from purple sweet potatoes.
基金This work was supported by the Doctorial Program Foundation of Institution of Higher Education of China(No. 97001005).This work was supported by the Doctorial Program Foundation of Institution of Higher Education of China(No. 97001005).
文摘The enzymatic hydrolysis of macromolecular gelatin with AS1.398 neutral protease, bromelain and their combinations was studied by estimating the molecular weights of their hydrolytic products. It was discovered that the products hydrolyzed by using combination enzymes had lower molecular weight than those obtained by using single ones, and the kind of enzymes, their combination mode and addition sequence are effective ways to control the molecular weights of gelatin hydrolyzates.
基金Project(2013zzts306)supported by the Fundamental Research Funds for the Central Universities of Central South University,ChinaProject(225)supported by the High Level Health Personnel in Hunan Province,China
文摘Porous titanium with porosity of 60% was prepared by metal injection molding（MIM）,and coated with gelatin sustained-release microspheres which were made by improved emulsified cold condensation method.The effects of porous titanium coated with insulin-like growth factor-1（IGF-1） and transforming growth factor-β1（TGF-β1） gelatin microspheres on the function of MG63 cells were evaluated in vitro.The results show that porous titanium coated with gelatin sustained-release microspheres has no cytotoxicity.The IGF-1 and TGF-β1 loading concentrations are positively correlative with the proliferation and differentiation of MG63 after co-culturing with the concentrations of IGF-1 and TGF-β1 gelatin microspheres in the range of 0.1-10 ng/mg and 0.25-2.5 ng/mg,respectively.The MG63 cells exhibit the best proliferation and differentiation with the IGF-1 and TGF-β1 loading concentrations of 10 ng/mg and 2.5 ng/mg,respectively.The joint application of IGF-1 and TGF-β1 group,which promote adhesion,proliferation and differentiation of MG63 cells,is superior to a single application group.
文摘Polyvinyl alcohol gelatin hydrogels were fabricated using genipin as a crosslinking agent for bone regeneration application. Optimized formulation of PVA-GE hydrogel was fabricated using genipin as crosslinking agent. Characterizations such as FTIR, morphology, porosity, pore size, degradation and swelling rate were investigated. Bone regeneration potential of optimized genipin cross-linked polyvinyl alcohol-gelatin (PVA20) hydrogels was assessed by implanting in rabbit’s femur defect for 1, 5 and 15 weeks period. Results showed interconnected porosity as observed in scanning electron microscopy and successful crosslinking as confirmed by FTIR analysis. Increased porosity (92% ± 2.46%) and pore size distribution (100 - 200 μm) were also observed as well as decrease in swelling rate (426% ± 10.50%). Bone formation was evident in micro-CT after 5 and 15 days of in vivo implantation period. Micro-CT analysis showed 32.67% increased bone formation of PVA-GE hydrogel defect compared with negative control after 15 weeks of in-vivo implantation. Histological analyses showed no inflammatory reaction post implantation and increase in cell matrix formation after 5 and 15 weeks. The combined physical and chemical method of crosslinking promises improved mechanical properties of PVA-GE hydrogel making it a potential scaffold for bone tissue engineering applications.
基金supported by the National Hi-Tech Research and Development Program(863)of China(No.2007AA02Z218)the Open Project Program of Key Lab-oratory of Eco-Textiles,Jiangnan University,Ministry of Education,China(No.KLET0625) the Youth Fundof Jiangnan University(No.2006LQN002).
文摘To achieve effective decolorization of reactive dyes,laccase immobilization was investigated.Laccase 0.2%(m/V)(Denilite IIS) was trapped in beads of alginate/gelatin blent with polyethylene glycol(PEG),and then the supporters were activated by cross-linking with glutaraldehyde.The results of repeated batch decolorization showed that gelatin and appropriate concentration of glutaraldehyde accelerated the decolorization of Reactive Red B-3BF(RRB);PEG had a positive effect on enzyme stability and led to an inc...
文摘Paddy rice samples were parboiled by soaking at 65℃ for 180 min and steaming at 96℃ for 2–10 min,and then dried to achieve the final moisture content of 11% ± 1%. The degree of starch gelatinization （DSG） andseveral quality attributes （head rice yield （HRY）, color value and hardness） of parboiled rice were measured.Results showed that DSG （46.8%–77.9%）, color value （18.08–19.04） and hardness （118.6–219.2 N） allincreased following steaming. In contrast, the HRY increased （64.8%–67.1%） for steaming times between 2–4min but decreased （67.1%–65.0%） for steaming times between 4–10 min. Linear relations between DSG andcolor value （R2 = 0.87）, and DSG and hardness （R2 = 0.88） were observed. The suitable DSG of parboiled riceleading to the highest HRY was found to be 62.5%, obtained following 4 min of steaming.
基金supported by the National Basic Research Program of China(“973 Program”,No.2012CB619100)the National Natural Science Foundation of China(Grant No.51372085)+1 种基金the Guangdong–Hongkong Common Technology Bidding Project(No.2013B010136003)the Postdoctoral Science Foundation of China(No.2013M542172)
文摘Gelatin/Alginate hydrogels were engineered for bioplotting in tissue engineering.One major drawback of hydrogel scaffolds is the lack of adequate mechanical properties.In this study,using a bioplotter,we constructed the scaffolds with different pore architectures by deposition of gelatin/alginate hydrogels layerby-layer.The scaffolds with different crosslinking degree were obtained by post-crosslinking methods.Their physicochemical properties,as well as cell viability,were assessed.Different crosslinking methods had little influence on scaffold architecture,porosity,pore size and distribution.By contrast,the water absorption ability,degradation rate and mechanical properties of the scaffolds were dramatically affected by treatment with various concentrations of crosslinking agent(glutaraldehyde).The crosslinking process using glutaraldehyde markedly improved the stability and mechanical strength of the hydrogel scaf-folds.Besides the post-processing methods,the pore architecture can also evidently affect the mechanical properties of the scaffolds.The crosslinked gelatin/alginate scaffolds showed a good potential to encap-sulate cells or drugs.
文摘The objective of this research was to develop gelatin microspheres（GMSs） and study their properties for embolization.The GMSs were prepared by emulsion chemical crosslinking method.The morphology and particle size of GMSs were observed under an optical microscope.The ratio of water absorption and GMSs swelling ratio were measured.The elasticity of GMSs was studied by TMS-Pro food texture analyzer.The deliverability of GMSs through catheter was investigated with a new device designed in this study.Finally,the acetone residue was determined by headspace capillary gas chromatography.The dried GMSs were elliptic with corrugated surface and the wet GMSs were round with smooth surface.The average diameter of dried GMSs was 430.5 μm with a range of 100-1000 μm,and that of wet GMSs was 601.2 μm with a range of 150-1425 μm.The maximum ratio of water absorption was 590.7% and the average swelling ratio was 103.0%.The elasticity of GMSs was proven to be appropriate for embolization.Three subgroups of wet GMSs（100-450 μm,450-700 μm and 700-940 μm） were delivered through catheter with acceptable pressure.The content of residual acetone in GMSs was less than the minimum limit in the Chinese Pharmacopoeia 2010.Therefore,we concluded that the GMSs were suitable for embolization according to the applicable properties in vitro.And the methods for assessing the properties of GMSs in this study were going to be useful in the evaluation of embolic microspheres.
