Gracilaria tenuistipitata var Liui were mono cultivated and co cultivated with Pinctada martensii under high (33) and low (21) salinity conditions in laboratory. The daily growth rate of the alga was determined. Tissu...Gracilaria tenuistipitata var Liui were mono cultivated and co cultivated with Pinctada martensii under high (33) and low (21) salinity conditions in laboratory. The daily growth rate of the alga was determined. Tissue carbon and nitrogen contents, the yield and fractional composition of agar were analyzed. Results showed that: 1. Gracilaria grew better under low salinity conditions, the daily growth rate was twice that under high salinity conditions. Co cultivated algae grew faster than mono cultivated algae under low salinity conditions, the daily growth rate was about 37.6% higher. 2. Compared with mono cultivated algae, tissue nitrogen contents of co cultivated algae were higher, while the C:N ratios were much lower. 3. The agar yields of co cultivated algae were much lower than those of mono cultivated algae. Agar yield was found to be negatively correlated to the tissue nitrogen contents, and positively correlated to the C:N ratios. 4. The highest fractional yields obtained from co cultivated algae were extracted with 40% ethanol, while from mono cultivated algae, the highest fractional yields obtained were extracted with distilled water at room temperature.展开更多
Objective To explore the proliferation-promoting effect of bovine mammary gland epithelial cells(BMECs)co-cultured with umbilical cord mesenchymal stem cells(UC-MSCs)in serum-free culture mediuum.Methods Bovine UC-MSC...Objective To explore the proliferation-promoting effect of bovine mammary gland epithelial cells(BMECs)co-cultured with umbilical cord mesenchymal stem cells(UC-MSCs)in serum-free culture mediuum.Methods Bovine UC-MSCs and BMECs were selected for co-culturing in direct or indirect contact.In the direct contact culture groups,UC-MSCs and BMECs were co-cultured at concentration ratios of 2∶1,1∶1,1∶2,1∶3,1∶4,1∶5,and 1:10,respectively.In the indirect contact culture group,the supernatant of UC-MSCs was used as the conditioned medium to resuspend BMECs.In the control groups,UC-MSCs and BMECs were cultured alone.The cell growth status in each group was observed at 0,4,8,12,24,36,48,60,72 h after culture,and cell proliferation was detected by cell counting kit-8(CCK-8)assay.Results At 48 h,the optical density of the conditioned medium-BMECs group was significantly higher compared with the control groups(P〈0.05).Meanwhile,the optical density in the direct contact group at a concentration ratio of 1∶2 reached the peak,which was extremely significantly higher compared with the control groups(P〈0.01)and significantly higher compared with the other direct contact culture groups and the conditioned medium-BMECs group(P〈0.05).Conclusions Co-culture of UC-MSCs and BMECs in serum-free culture medium is capable to promote the proliferation of BMECs,and the co-culture by cell-to-cell contact has a better effect.The optimal concentration ratio of UC-MSCs to BMECs is 1∶2,and the optimal culture time is 48 h.展开更多
This study investigates the environmental benefits of integrated rice-duck co-culture as a sustainable management practice in rice production.Through a comprehensive meta-analysis and life cycle assessment,we find not...This study investigates the environmental benefits of integrated rice-duck co-culture as a sustainable management practice in rice production.Through a comprehensive meta-analysis and life cycle assessment,we find notable differences of co-cultures from monocultures,including a 22%reduction in methane(CH_(4))emissions,a 34%increase in nitrous oxide(N_(2)O)emissions,a 2.8%rise in rice yields,and>100%increase in profits.Accounting for these on-site differences and the indirect emissions embedded in agricultural inputs,the carbon footprint of rice-duck co-culture per hectare of land is estimated to be 9%lower than that of monoculture.In addition to reducing the life-cycle greenhouse gas emissions of rice production and enhancing rice productivity,rice-duck co-cultures produce additional protein that can be used to displace industrially farmed duck or other meats.Scenario analysis indicates that scaling up rice-duck co-culture system in China could lead to important greenhouse gas savings.Overall,our study suggests that promoting rice-duck co-culture can contribute toward sustainable rice production and dietary change.展开更多
In vitro liver disease modelling,a rapidly evolving field,has become a multidimensional endeavour aimed at more precisely and effectively recapitulating the complexity of hepatic pathophysiology.This review systematic...In vitro liver disease modelling,a rapidly evolving field,has become a multidimensional endeavour aimed at more precisely and effectively recapitulating the complexity of hepatic pathophysiology.This review systematically outlines the essential structural and cellular components of the liver as foundational elements for model design.Emphasising pathophysiological states rather than disease hallmarks,we discuss key liver injury paradigms,including hepatic steatosis,drug-induced hepatotoxicity,fibrogenesis,tumourigenesis and cholestatic injury.Each section integrates cellular mechanisms with model development strategies,highlighting advances in co-culture systems,multicellular organoids and liver-on-a-chip platforms.Although challenges persist,emerging platforms are increasingly capable of capturing multicellular crosstalk,structural heterogeneity and injury-response dynamics.Moving forward,model utility will depend not only on structural mimicry but on the ability to produce biologically meaningful outputs under experimentally controlled conditions.展开更多
文摘Gracilaria tenuistipitata var Liui were mono cultivated and co cultivated with Pinctada martensii under high (33) and low (21) salinity conditions in laboratory. The daily growth rate of the alga was determined. Tissue carbon and nitrogen contents, the yield and fractional composition of agar were analyzed. Results showed that: 1. Gracilaria grew better under low salinity conditions, the daily growth rate was twice that under high salinity conditions. Co cultivated algae grew faster than mono cultivated algae under low salinity conditions, the daily growth rate was about 37.6% higher. 2. Compared with mono cultivated algae, tissue nitrogen contents of co cultivated algae were higher, while the C:N ratios were much lower. 3. The agar yields of co cultivated algae were much lower than those of mono cultivated algae. Agar yield was found to be negatively correlated to the tissue nitrogen contents, and positively correlated to the C:N ratios. 4. The highest fractional yields obtained from co cultivated algae were extracted with 40% ethanol, while from mono cultivated algae, the highest fractional yields obtained were extracted with distilled water at room temperature.
文摘Objective To explore the proliferation-promoting effect of bovine mammary gland epithelial cells(BMECs)co-cultured with umbilical cord mesenchymal stem cells(UC-MSCs)in serum-free culture mediuum.Methods Bovine UC-MSCs and BMECs were selected for co-culturing in direct or indirect contact.In the direct contact culture groups,UC-MSCs and BMECs were co-cultured at concentration ratios of 2∶1,1∶1,1∶2,1∶3,1∶4,1∶5,and 1:10,respectively.In the indirect contact culture group,the supernatant of UC-MSCs was used as the conditioned medium to resuspend BMECs.In the control groups,UC-MSCs and BMECs were cultured alone.The cell growth status in each group was observed at 0,4,8,12,24,36,48,60,72 h after culture,and cell proliferation was detected by cell counting kit-8(CCK-8)assay.Results At 48 h,the optical density of the conditioned medium-BMECs group was significantly higher compared with the control groups(P〈0.05).Meanwhile,the optical density in the direct contact group at a concentration ratio of 1∶2 reached the peak,which was extremely significantly higher compared with the control groups(P〈0.01)and significantly higher compared with the other direct contact culture groups and the conditioned medium-BMECs group(P〈0.05).Conclusions Co-culture of UC-MSCs and BMECs in serum-free culture medium is capable to promote the proliferation of BMECs,and the co-culture by cell-to-cell contact has a better effect.The optimal concentration ratio of UC-MSCs to BMECs is 1∶2,and the optimal culture time is 48 h.
基金supported by the National Natural Science Foundation of China(grant number:52370193)
文摘This study investigates the environmental benefits of integrated rice-duck co-culture as a sustainable management practice in rice production.Through a comprehensive meta-analysis and life cycle assessment,we find notable differences of co-cultures from monocultures,including a 22%reduction in methane(CH_(4))emissions,a 34%increase in nitrous oxide(N_(2)O)emissions,a 2.8%rise in rice yields,and>100%increase in profits.Accounting for these on-site differences and the indirect emissions embedded in agricultural inputs,the carbon footprint of rice-duck co-culture per hectare of land is estimated to be 9%lower than that of monoculture.In addition to reducing the life-cycle greenhouse gas emissions of rice production and enhancing rice productivity,rice-duck co-cultures produce additional protein that can be used to displace industrially farmed duck or other meats.Scenario analysis indicates that scaling up rice-duck co-culture system in China could lead to important greenhouse gas savings.Overall,our study suggests that promoting rice-duck co-culture can contribute toward sustainable rice production and dietary change.
基金supported by the German Research Foundation(DFG Ta434/8-1,CRC/TR 412 Project-ID 535081457 and SFB1382,Project-ID 403224013 and ID 556479455).
文摘In vitro liver disease modelling,a rapidly evolving field,has become a multidimensional endeavour aimed at more precisely and effectively recapitulating the complexity of hepatic pathophysiology.This review systematically outlines the essential structural and cellular components of the liver as foundational elements for model design.Emphasising pathophysiological states rather than disease hallmarks,we discuss key liver injury paradigms,including hepatic steatosis,drug-induced hepatotoxicity,fibrogenesis,tumourigenesis and cholestatic injury.Each section integrates cellular mechanisms with model development strategies,highlighting advances in co-culture systems,multicellular organoids and liver-on-a-chip platforms.Although challenges persist,emerging platforms are increasingly capable of capturing multicellular crosstalk,structural heterogeneity and injury-response dynamics.Moving forward,model utility will depend not only on structural mimicry but on the ability to produce biologically meaningful outputs under experimentally controlled conditions.
