在地方经济蓬勃发展与医药行业深刻变革的宏观背景下,培育高素质的临床试验协调员(Clinical Research Coordinator,简称CRC),已成为顺应社会需求、推进健康中国建设的关键举措。本文以四川护理职业学院针对药学专业开展的临床试验项目...在地方经济蓬勃发展与医药行业深刻变革的宏观背景下,培育高素质的临床试验协调员(Clinical Research Coordinator,简称CRC),已成为顺应社会需求、推进健康中国建设的关键举措。本文以四川护理职业学院针对药学专业开展的临床试验项目化教学试点为切入点,深入剖析其构建的创新医教协同培养模式,从人才培养实践、培养的关键问题与应对策略及培养成效等方面进行介绍。此培养模式在实践中取得了积极的成效,显著提升了学生的就业竞争力,为其他地区在CRC人才培养方面提供了极具价值的参考范例,助力行业整体人才培养水平的提升。展开更多
Hesperidin is a dihydroflavonoids, accounting for more than 50% of the total flavonoids in Citri Reticulatae Pericarpium(CRP) of traditional Chinese medicine. It is an effective antioxidant and free radical scavenger ...Hesperidin is a dihydroflavonoids, accounting for more than 50% of the total flavonoids in Citri Reticulatae Pericarpium(CRP) of traditional Chinese medicine. It is an effective antioxidant and free radical scavenger that has anti-inflammatory, antiviral and hypoglycemic properties.The latest studies reported that hesperidin has a potential for novel coronavirus resistance. However, little is known about the synthesis regulation and accumulation site of hesperidin in plants. In this study, hesperidin synthase gene Crc1,6RhaT was cloned, and the protein can be completely transformed flavanone-7-O-glucoside into hesperidin in vitro and in vivo. Studies on biological characteristics of ovary walls and exocarps showed that the relative expression levels of the Crc1,6RhaT gene and protein decreased gradually with the development of citrus fruits, and the relative content of hesperidin firstly increased, then sequentially decreased. In situ hybridization results further revealed that Crc1,6RhaT transcription was mainly concentrated in the secretory cavity cells, which are revealed to be the site of flavonoid synthesis.Immunocytochemistry localization results showed that the Crc1,6RhaT was mainly located in the endoplasmic reticulum, nucleus and vacuole of secretory cells. We inferred that the Crc1,6RhaT was synthesized in the endoplasmic reticulum, then was transported into the vacuoles through enlarged vesicles at the end of the endoplasmic reticulum. Our results not only revealed that Crc1,6RhaT may be involved in the synthesis of hesperidin of the main bioactive substance in the medicinal plant Citrus reticulata ‘Chachi' fruit, but also provided novel insights into the main subcellular sites of hesperidin biosynthesis in vacuoles.展开更多
文摘在地方经济蓬勃发展与医药行业深刻变革的宏观背景下,培育高素质的临床试验协调员(Clinical Research Coordinator,简称CRC),已成为顺应社会需求、推进健康中国建设的关键举措。本文以四川护理职业学院针对药学专业开展的临床试验项目化教学试点为切入点,深入剖析其构建的创新医教协同培养模式,从人才培养实践、培养的关键问题与应对策略及培养成效等方面进行介绍。此培养模式在实践中取得了积极的成效,显著提升了学生的就业竞争力,为其他地区在CRC人才培养方面提供了极具价值的参考范例,助力行业整体人才培养水平的提升。
基金supported by the National Natural Science Foundation of China (Grant No.32270381)Natural Science Foundation of Guangdong (Grant No.2022A1515011086)+2 种基金Key Realm R&D Program of Guangdong Province (Grant No.2020B020221001)Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (Grant No.2019KJ125)Research Fund of Maoming Branch,Guangdong Laboratory for Lingnan Modern Agriculture (Grant No.2022KF009)。
文摘Hesperidin is a dihydroflavonoids, accounting for more than 50% of the total flavonoids in Citri Reticulatae Pericarpium(CRP) of traditional Chinese medicine. It is an effective antioxidant and free radical scavenger that has anti-inflammatory, antiviral and hypoglycemic properties.The latest studies reported that hesperidin has a potential for novel coronavirus resistance. However, little is known about the synthesis regulation and accumulation site of hesperidin in plants. In this study, hesperidin synthase gene Crc1,6RhaT was cloned, and the protein can be completely transformed flavanone-7-O-glucoside into hesperidin in vitro and in vivo. Studies on biological characteristics of ovary walls and exocarps showed that the relative expression levels of the Crc1,6RhaT gene and protein decreased gradually with the development of citrus fruits, and the relative content of hesperidin firstly increased, then sequentially decreased. In situ hybridization results further revealed that Crc1,6RhaT transcription was mainly concentrated in the secretory cavity cells, which are revealed to be the site of flavonoid synthesis.Immunocytochemistry localization results showed that the Crc1,6RhaT was mainly located in the endoplasmic reticulum, nucleus and vacuole of secretory cells. We inferred that the Crc1,6RhaT was synthesized in the endoplasmic reticulum, then was transported into the vacuoles through enlarged vesicles at the end of the endoplasmic reticulum. Our results not only revealed that Crc1,6RhaT may be involved in the synthesis of hesperidin of the main bioactive substance in the medicinal plant Citrus reticulata ‘Chachi' fruit, but also provided novel insights into the main subcellular sites of hesperidin biosynthesis in vacuoles.
