Systematically analyzing the impact mechanisms of policy on Land Use Conflict(LUC)is crucial for constructing effective conflict mitigation strategies.However,previous research on how policy influences LUC remains rel...Systematically analyzing the impact mechanisms of policy on Land Use Conflict(LUC)is crucial for constructing effective conflict mitigation strategies.However,previous research on how policy influences LUC remains relatively limited.Focusing on the indirect driving role of policy on LUC,this study proposed County Development Level(CDL)under Major Function Oriented Zone Planning(MFOZP)guidance as an intermediary variable,bridging the implicit influence of MFOZP and the explicit changes in LUC.Using the Beijing-Tianjin-Hebei(BTH)region in China as a case study,we analyzed the spatio-temporal evolution characteristics of LUC and CDL for the periods 2000-2010 and 2010-2020,before and after MFOZP implementation.Panel models and Geographically Weighted Regression(GWR)were employed to explore the mechanism by which CDL influences LUC under MFOZP guidance.The results show that:1)MFOZP implementation effectively alleviates land use pressure from regional development,with LUC continuously declining at a rate of 2.41%,while CDL exhibits slight growth(3.84%),during 2010-2020.2)Under MFOZP guidance,CDL reduces pressure on Land Use Structure Conflict(LUSC)and Land Use Process Conflict(LUPC),enhances its inhibitory effect on Land Use Function Conflict(LUFC),and significantly contributes to LUC coordination,with notable spatial heterogeneity.3)The coupling relationship between CDL and LUC has improved post-implementation.Based on this,tailored LUC coordination strategies are proposed for different functional zones.This study confirms the effectiveness of MFOZP in coordinating LUC and provides a scientific reference for LUC research under policy frameworks and the governance of LUC in the BTH region.展开更多
目的：观察蝎毒多肽提取物（polypeptide extract from scorpion venom，PESV）对H22肝癌荷瘤小鼠化疗期间再增殖的抑制作用并探讨可能的机制。方法：96只Balb／c小鼠皮下接种小鼠肝癌H22细胞，随机分为模型组、PESV高、低剂量组和衙瘤...目的：观察蝎毒多肽提取物（polypeptide extract from scorpion venom，PESV）对H22肝癌荷瘤小鼠化疗期间再增殖的抑制作用并探讨可能的机制。方法：96只Balb／c小鼠皮下接种小鼠肝癌H22细胞，随机分为模型组、PESV高、低剂量组和衙瘤对照组。以5．Fu对荷瘤小鼠进行化疗建立再增殖模型。按不同的用药方法对4组小鼠进行干预，每周测量肿瘤体积2次。各组每7d处死6只小鼠，实验共进行35d。以免疫组织化学方法检测各组肿瘤组织CD105，PCNA，VEGF，PDGF蛋白水半表达。以CD105标记微血管，计算微血管密度（MVD）。结果：荷瘤对照组肿瘤体积在13—24d增加迅速，荷瘤对照组小鼠在第27天全部死亡，模型组肿瘤体积在17d前增长较快，在第17～22天增加较慢，之后体积增加又较快，在第31天全部死亡。PESV高、低剂量组肿瘤体积全程缓慢增加，体积在17d以后显著低于模型组，PESV高低剂量组之间仅在第17天存在差异（P〈0．05）。免疫组织化学检测湿示，模型组肿瘤组织第31天PCNA表达水平高于第21，28天（P〈0．01），PESV高、低剂量组表达水平显著低于模型组（P〈0．01），PESV高、低剂量组仅在第17天存在显著差异（P〈0．05）。免疫组织化学检测显示，与PESV高、低剂量组相比，模型组CD105-MVD在第21，28天（P〈0．05），第35天（P〈0．01）存在显著差异，PESV高低剂量组之间无差别。模型组VEGF表达第35天高于第21，28天（P〈0．05），PESV高、低剂量VEGF表达在第21，28，35天表达水平均显著低于模型组（P〈0．01），PESV高低剂量组无差别。模型组肿瘤PDGF表达水平逐渐下降，PESV高，低组存第21天表达水平最低，之后逐渐增高，PESV高低剂量组之间在第35天存在显著差异（P〈0．01）。相关性分析显示，肿瘤组织VEGF表达水平与肿瘤组织CD105-MVD呈正相关（r=0．669）。结论：PESV可抑制H22肿瘤在化疔期间再增殖作用，其机制可能是抑制血管生成和使肿瘤血管正常化。展开更多
基金Under the auspices of the National Key Research and Development Program of China(No.2018YFD1100803)the Fundamental Research Fund for the Central Universities(Ph.D.Top Innovative Talents Fund of CUMTB)(No.BBJ2024029)。
文摘Systematically analyzing the impact mechanisms of policy on Land Use Conflict(LUC)is crucial for constructing effective conflict mitigation strategies.However,previous research on how policy influences LUC remains relatively limited.Focusing on the indirect driving role of policy on LUC,this study proposed County Development Level(CDL)under Major Function Oriented Zone Planning(MFOZP)guidance as an intermediary variable,bridging the implicit influence of MFOZP and the explicit changes in LUC.Using the Beijing-Tianjin-Hebei(BTH)region in China as a case study,we analyzed the spatio-temporal evolution characteristics of LUC and CDL for the periods 2000-2010 and 2010-2020,before and after MFOZP implementation.Panel models and Geographically Weighted Regression(GWR)were employed to explore the mechanism by which CDL influences LUC under MFOZP guidance.The results show that:1)MFOZP implementation effectively alleviates land use pressure from regional development,with LUC continuously declining at a rate of 2.41%,while CDL exhibits slight growth(3.84%),during 2010-2020.2)Under MFOZP guidance,CDL reduces pressure on Land Use Structure Conflict(LUSC)and Land Use Process Conflict(LUPC),enhances its inhibitory effect on Land Use Function Conflict(LUFC),and significantly contributes to LUC coordination,with notable spatial heterogeneity.3)The coupling relationship between CDL and LUC has improved post-implementation.Based on this,tailored LUC coordination strategies are proposed for different functional zones.This study confirms the effectiveness of MFOZP in coordinating LUC and provides a scientific reference for LUC research under policy frameworks and the governance of LUC in the BTH region.
文摘目的：观察蝎毒多肽提取物（polypeptide extract from scorpion venom，PESV）对H22肝癌荷瘤小鼠化疗期间再增殖的抑制作用并探讨可能的机制。方法：96只Balb／c小鼠皮下接种小鼠肝癌H22细胞，随机分为模型组、PESV高、低剂量组和衙瘤对照组。以5．Fu对荷瘤小鼠进行化疗建立再增殖模型。按不同的用药方法对4组小鼠进行干预，每周测量肿瘤体积2次。各组每7d处死6只小鼠，实验共进行35d。以免疫组织化学方法检测各组肿瘤组织CD105，PCNA，VEGF，PDGF蛋白水半表达。以CD105标记微血管，计算微血管密度（MVD）。结果：荷瘤对照组肿瘤体积在13—24d增加迅速，荷瘤对照组小鼠在第27天全部死亡，模型组肿瘤体积在17d前增长较快，在第17～22天增加较慢，之后体积增加又较快，在第31天全部死亡。PESV高、低剂量组肿瘤体积全程缓慢增加，体积在17d以后显著低于模型组，PESV高低剂量组之间仅在第17天存在差异（P〈0．05）。免疫组织化学检测湿示，模型组肿瘤组织第31天PCNA表达水平高于第21，28天（P〈0．01），PESV高、低剂量组表达水平显著低于模型组（P〈0．01），PESV高、低剂量组仅在第17天存在显著差异（P〈0．05）。免疫组织化学检测显示，与PESV高、低剂量组相比，模型组CD105-MVD在第21，28天（P〈0．05），第35天（P〈0．01）存在显著差异，PESV高低剂量组之间无差别。模型组VEGF表达第35天高于第21，28天（P〈0．05），PESV高、低剂量VEGF表达在第21，28，35天表达水平均显著低于模型组（P〈0．01），PESV高低剂量组无差别。模型组肿瘤PDGF表达水平逐渐下降，PESV高，低组存第21天表达水平最低，之后逐渐增高，PESV高低剂量组之间在第35天存在显著差异（P〈0．01）。相关性分析显示，肿瘤组织VEGF表达水平与肿瘤组织CD105-MVD呈正相关（r=0．669）。结论：PESV可抑制H22肿瘤在化疔期间再增殖作用，其机制可能是抑制血管生成和使肿瘤血管正常化。
