[Objective] The research aimed to lay a foundation for the screening of cell lines producing secondary metabolites of Brucea javanica(L.)Merr.[Method] The insecticidal activities of the extracts from branch and 3 diff...[Objective] The research aimed to lay a foundation for the screening of cell lines producing secondary metabolites of Brucea javanica(L.)Merr.[Method] The insecticidal activities of the extracts from branch and 3 different types of calluses of Brucea javanica(L.)Merr.was detected through methods of leaf disc and potted seedlings against the diamond back moth.[Result] Extracts from four kinds of Brucea javanica(L.)Merr.tissues assumed both the activities of antifeedant and oviposition deterrency against the diamond back moth.Antifeedant effect of extracts was in turn the callus C< callus B< callus A< branches.Oviposition deterrency activity of the extracts was in turn the callus A> branch > callus B>callus C.The insecticidal activities of callus A and B were higher than that of the callus C.[Conclusion] The results show that insecticidal activity of callus and its growth rate is inversely proportional.展开更多
Trichomes （plant hairs） are present on nearly all land plants and are known to play important roles in plant protection, specifically against insect herbivory, drought, and UV radiation. The identification of quanti...Trichomes （plant hairs） are present on nearly all land plants and are known to play important roles in plant protection, specifically against insect herbivory, drought, and UV radiation. The identification of quantitative trait loci （QTL） associated with trichome density should help to interpret the molecular genetic mechanism of soybean trichome density. 184 recombinant inbred lines （RILs）, derived from a cross between soybean cultivars Kefeng 1 and Nannong 1138-2 were used as segregating population for evaluation of TDU （trichome density on the upper surface of leaf blade） and TDD （trichome density on the downer surface of leaf blade）. A total of 15 QTL were detected on molecular linkage groups （MLG） A2, Dla, Dlb, E and H by composite interval mapping （CIM） and among all the QTL, qtuA2-1, qtuD 1 a-1, qtuD lb-2, qtuH-2 qtuE-1, qtdDlb-2, and qtdH- 2 were affirmed by multiple interval mapping （MIM）. The contribution ofphenotypic variance of qtuH-2 was 31.81 and 29.4% by CIM and MIM, respectively, suggesting it might be major gene Ps loci. Only 10 pairs of main QTL interactions for TDU were detected, explained a range of 0.2-5.1% of phenotypic variations for each pair for a total of 22.8%. The QTL on MLG Dlb affecting trichome density were mapped near to Rsc-7 conditioning resistance to SMV （soybean mosaic virus）. This study showed that the genetic mechanism of trichome density was the mixed major gene and polygene inheritance, and also suggested that the causal nature between trichome density and other agronomic traits.展开更多
Syzygium myrsinifolium(Hance)Merr.et Perry is a unique plant resource on Hainan Island,and there are few reports on its chemical constituents.In the present study,we identified the major chemical constituents in the l...Syzygium myrsinifolium(Hance)Merr.et Perry is a unique plant resource on Hainan Island,and there are few reports on its chemical constituents.In the present study,we identified the major chemical constituents in the leaves of Syzygium myrsinifolium(Hance)Merr.et Perry using ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS).The chemical constituents in Syzygium myrsinifolium(Hance)Merr.et Perry were identified rapidly based on the accurate relative molecular mass and combined with literature data and reference substances.A total of 20 compounds,including organic acids,phenolic acids,and flavonoids,were identified.Among these 20 compounds,four organic acids,eight phenolic acids,seven flavonoids,and one coumarin were reported from the leaves of Syzygium myrsinifolium(Hance)Merr.et Perry for the first time.The established method was rapid and accurate,and some chemical constituents in the leaves of Syzygium myrsinifolium(Hance)Merr.et Perry have been identified.This research provided an experimental reference for the study of the constituents and utilization of Syzygium myrsinifolium(Hance)Merr.et Perry.展开更多
The genetic analysis of soybean isoflavone content and its components were carried out based on the NC Ⅱ matingdesign in eight soybean varieties. The results showed that the isoflavone contents and its components of ...The genetic analysis of soybean isoflavone content and its components were carried out based on the NC Ⅱ matingdesign in eight soybean varieties. The results showed that the isoflavone contents and its components of soybean seedare quite differences among the tested materials, the contents of isoflavone and daidzein are controlled not only byadditive effects and but also by non-additive effects, while the content of genistin is dominated by non-additive effects,and genistein, glycitin and daidzin are mainly controlled by additive effects. There are significant differences in thecontents of isoflavone and its components among the combinations derived from different parents. Results also indicatedthat the tested traits are negatively heterosis except for the contens of daidzein and daidzin are positively heterosis basedon the data of the GCA and SCA in average heterosis values. In this research we have a suggestion that soybean varietywith high isoflavone should be used as one of the parents in the breeding program, and it is the best choice that thecombinations crossed between two high isoflavone varieties or a high variety and a low one.展开更多
Soybean seed products contain isoflavones （genistein, daidzein, and glycitein） that display biological effects when ingested by humans and animals. These effects are species, dose and age dependent. Therefore, the c...Soybean seed products contain isoflavones （genistein, daidzein, and glycitein） that display biological effects when ingested by humans and animals. These effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key factor to the biological effect. Our objective was to identify the genetic effects that underlie the isoflavone content in soybean seeds. A genetic model for quantitative traits of seeds in diploid plants was applied to estimate the genetic main effects and genotype x environment （GE） interaction effects for the isoflavone content （IC） of soybean seeds by using two years experimental data with an incomplete diallel mating design of six parents. Results showed that the IC of soybean seeds was simultaneously controlled by the genetic effects of maternal, embryo, and cytoplasm, of which maternal genetic effects were most important, followed by embryo and cytoplasmic genetic effects. The main effects of different genetic systems on IC trait were more important than environment interaction effects. The strong dominance effects on isoflavone from residual was made easily by environment conditions. Therefore, the improvement of the IC of soybean seeds would be more efficient when selection is based on maternal plants than that on the single seed. Maternal heritability （65.73%） was most important for IC, followed by embryo heritability （25.87%） and cytoplasmic heritability （8.39%）. Based on predicated genetic effects, Yudou 29 and Zheng 90007 were better than other parents for increasing IC in the progeny and improving the quality of soybean, The significant effects of maternal and embryo dominance effects in variance show that the embryo heterosis and maternal heterosis are existent and uninfluenced by environment interaction effects.展开更多
Acanthopanax trifoliatus( L.) Merr is a common traditional Chinese herbal medicine in central and southern regions of China. It has high medicinal and edible value and mainly contains flavonoids,polysaccharides,saponi...Acanthopanax trifoliatus( L.) Merr is a common traditional Chinese herbal medicine in central and southern regions of China. It has high medicinal and edible value and mainly contains flavonoids,polysaccharides,saponins and volatile components. Its active extracts have antioxidant,anti-inflammatory,anti-fatigue,and analgesic effects. From the biological characteristics,effective chemical components,extraction methods,cultivation techniques,pharmacological effects,toxicological activities,and product development status,this paper reviewed in detail the research status of A. trifoliatus in China and abroad,to provide a scientific basis for in-depth research and development,and utilization of A. trifoliatus.展开更多
[Objectives] This study aimed to investigate the hypolipidemic effects of different processed products of Ilex hainanensis Merr to provide experimental basis for the research on the hypolipidemic mechanism of I. haina...[Objectives] This study aimed to investigate the hypolipidemic effects of different processed products of Ilex hainanensis Merr to provide experimental basis for the research on the hypolipidemic mechanism of I. hainanensis Merr. [Methods] SD rats were fed high-fat diet to establish hyperlipidemia rat models. The hyperlipidemia rats were administered with different processed products of I. hainanensis Merr by gavage to investigate the preventive and therapeutic effects of the medicinal material. [Results] The levels of total cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterol(LDL-C) in the rats fed high-fat diet were significantly higher than those in of the control group(P<0.01), indicating successful modeling. Among different processing methods, the hypolipidemic effects were in the order as baking=traditional processing=frying=microwaving>drying in shade. Among different baking times, the hypolipidemic effects ranked as 180 ℃>200 ℃=220 ℃. Among different baking times, the hypolipidemic effects ranked as 20 min=40 min>30 min. The hypolipidemic effects of processed product of I. hainanensis Merr with different amounts of active ingredients were different. [Conclusions] The processed products of I. hainanensis Merr all had hypolipidemic effects, but the effects were different. This study will provide a scientific basis for expanding the resources of I. hainanensis Merr.展开更多
A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference ...A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference maps of protein extracts from standard petals(SP), lateral wings(LW), keel petals(KP), and reproductive organs(RO)(a mixture of stamen and carpel) were obtained. Protein expression in the three petal types was compared using Image Master TM 2 D platinum 6.0 software. This indicated that the proportion of homologous proteins between SP and LW was 59.27%, between SP and KP was 61.48%, and between LW and KP was 60.05%. Within a mass range of 6.5-200.0 ku and pH 4.0-7.0, approximately 590, 646, 544, and 700 protein spots were detected in SP, LW, KP, and RO, respectively. A total of 82 differentially expressed proteins were detected. Sixty-four of these detected spots were differentially expressed and showed more than 2-fold changes in abundance; of these 64 proteins, 26 showed increased expression and 38 showed decreased expression. Among these spots, single organ-specific proteins were also identified.They were ID 49(60.9 ku), ID 45(50.0 ku), and ID 46(40.5 ku) in RO, ID 98(42.0 ku) in SP, and ID 05(29.0 ku) in KP. A total of 14 protein spots from 82 differentially expressed proteins were identified with LC-MS/MS. Further protein identification was conducted using the SwissProt and NCBInr databases. The identified proteins and their putative functions were discussed further. This was the first study reporting the comparison of petal protein profiles of soybean florets using proteomics tools.展开更多
[Objectives]To establish a method for determining the naringin content in the peel of Guangxi Citrus maxima(Burm.)Merr.[Methods]The high performance liquid chromatography(HPLC)method was applied.The chromatographic co...[Objectives]To establish a method for determining the naringin content in the peel of Guangxi Citrus maxima(Burm.)Merr.[Methods]The high performance liquid chromatography(HPLC)method was applied.The chromatographic column was Agilent HC-C18(4.6 mm×250 mm,5μm);the mobile phase was acetonitrile-0.1%phosphoric acid(18∶82);the flow rate was 1.0 mL/min;the column temperature was 25℃;the detection wavelength was 283 nm.[Results]Naringin showed a good linear relationship in the range of 0.164-3.27μg,r=0.9999.The average recovery rate was 98.66%,and RSD=1.80%(n=6).[Conclusions]This method is simple,feasible,reproducible,and accurate,so it can be used for the determination of naringin content in the peel of Guangxi C.maxima.展开更多
基金Supported by Guangxi Science Foundation(0639027)Science and Technology Developmental Fund of Guangxi Academy of Agricultural Sciences(2006025)~~
文摘[Objective] The research aimed to lay a foundation for the screening of cell lines producing secondary metabolites of Brucea javanica(L.)Merr.[Method] The insecticidal activities of the extracts from branch and 3 different types of calluses of Brucea javanica(L.)Merr.was detected through methods of leaf disc and potted seedlings against the diamond back moth.[Result] Extracts from four kinds of Brucea javanica(L.)Merr.tissues assumed both the activities of antifeedant and oviposition deterrency against the diamond back moth.Antifeedant effect of extracts was in turn the callus C< callus B< callus A< branches.Oviposition deterrency activity of the extracts was in turn the callus A> branch > callus B>callus C.The insecticidal activities of callus A and B were higher than that of the callus C.[Conclusion] The results show that insecticidal activity of callus and its growth rate is inversely proportional.
基金supported in part by a grant from the International Atomic Energy Agency (CPR-12988)Na-tional 973 Program of China (2004CB117206)+4 种基金National863 Program of China (2006AA10Z1C1)National Natu-ral Science Foundation of China (30771362 and30471094)the 111 Project from the Ministry of Edu-cation of China (B08025)the Key Program of Science and Technology of Shanxi Province of China (051017)the Scientific Research Foundation for Youth Aca-demic Leaders from University in Shanxi Province of China (200425)
文摘Trichomes （plant hairs） are present on nearly all land plants and are known to play important roles in plant protection, specifically against insect herbivory, drought, and UV radiation. The identification of quantitative trait loci （QTL） associated with trichome density should help to interpret the molecular genetic mechanism of soybean trichome density. 184 recombinant inbred lines （RILs）, derived from a cross between soybean cultivars Kefeng 1 and Nannong 1138-2 were used as segregating population for evaluation of TDU （trichome density on the upper surface of leaf blade） and TDD （trichome density on the downer surface of leaf blade）. A total of 15 QTL were detected on molecular linkage groups （MLG） A2, Dla, Dlb, E and H by composite interval mapping （CIM） and among all the QTL, qtuA2-1, qtuD 1 a-1, qtuD lb-2, qtuH-2 qtuE-1, qtdDlb-2, and qtdH- 2 were affirmed by multiple interval mapping （MIM）. The contribution ofphenotypic variance of qtuH-2 was 31.81 and 29.4% by CIM and MIM, respectively, suggesting it might be major gene Ps loci. Only 10 pairs of main QTL interactions for TDU were detected, explained a range of 0.2-5.1% of phenotypic variations for each pair for a total of 22.8%. The QTL on MLG Dlb affecting trichome density were mapped near to Rsc-7 conditioning resistance to SMV （soybean mosaic virus）. This study showed that the genetic mechanism of trichome density was the mixed major gene and polygene inheritance, and also suggested that the causal nature between trichome density and other agronomic traits.
基金Natural Science Foundation of Hainan Province(Grant No.320QN302)the Central Public-interest Scientific Institution Basal Research Fund for the Chinese Academy of Tropical Agricultural Sciences(Grant No.1251632022005)+1 种基金the Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation(Grant No.ZX-2022002)Open Fund of Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables(Grant No.KFKT2021002)。
文摘Syzygium myrsinifolium(Hance)Merr.et Perry is a unique plant resource on Hainan Island,and there are few reports on its chemical constituents.In the present study,we identified the major chemical constituents in the leaves of Syzygium myrsinifolium(Hance)Merr.et Perry using ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS).The chemical constituents in Syzygium myrsinifolium(Hance)Merr.et Perry were identified rapidly based on the accurate relative molecular mass and combined with literature data and reference substances.A total of 20 compounds,including organic acids,phenolic acids,and flavonoids,were identified.Among these 20 compounds,four organic acids,eight phenolic acids,seven flavonoids,and one coumarin were reported from the leaves of Syzygium myrsinifolium(Hance)Merr.et Perry for the first time.The established method was rapid and accurate,and some chemical constituents in the leaves of Syzygium myrsinifolium(Hance)Merr.et Perry have been identified.This research provided an experimental reference for the study of the constituents and utilization of Syzygium myrsinifolium(Hance)Merr.et Perry.
基金This work was jointly spored by the grants from the Natural Science Foundation of Henan Province(0511030500)the National 863 Program of China(2004AA2111112)the N ational Natural Science Foundation of China(30490250).
文摘The genetic analysis of soybean isoflavone content and its components were carried out based on the NC Ⅱ matingdesign in eight soybean varieties. The results showed that the isoflavone contents and its components of soybean seedare quite differences among the tested materials, the contents of isoflavone and daidzein are controlled not only byadditive effects and but also by non-additive effects, while the content of genistin is dominated by non-additive effects,and genistein, glycitin and daidzin are mainly controlled by additive effects. There are significant differences in thecontents of isoflavone and its components among the combinations derived from different parents. Results also indicatedthat the tested traits are negatively heterosis except for the contens of daidzein and daidzin are positively heterosis basedon the data of the GCA and SCA in average heterosis values. In this research we have a suggestion that soybean varietywith high isoflavone should be used as one of the parents in the breeding program, and it is the best choice that thecombinations crossed between two high isoflavone varieties or a high variety and a low one.
文摘Soybean seed products contain isoflavones （genistein, daidzein, and glycitein） that display biological effects when ingested by humans and animals. These effects are species, dose and age dependent. Therefore, the content and quality of isoflavones in soybeans is a key factor to the biological effect. Our objective was to identify the genetic effects that underlie the isoflavone content in soybean seeds. A genetic model for quantitative traits of seeds in diploid plants was applied to estimate the genetic main effects and genotype x environment （GE） interaction effects for the isoflavone content （IC） of soybean seeds by using two years experimental data with an incomplete diallel mating design of six parents. Results showed that the IC of soybean seeds was simultaneously controlled by the genetic effects of maternal, embryo, and cytoplasm, of which maternal genetic effects were most important, followed by embryo and cytoplasmic genetic effects. The main effects of different genetic systems on IC trait were more important than environment interaction effects. The strong dominance effects on isoflavone from residual was made easily by environment conditions. Therefore, the improvement of the IC of soybean seeds would be more efficient when selection is based on maternal plants than that on the single seed. Maternal heritability （65.73%） was most important for IC, followed by embryo heritability （25.87%） and cytoplasmic heritability （8.39%）. Based on predicated genetic effects, Yudou 29 and Zheng 90007 were better than other parents for increasing IC in the progeny and improving the quality of soybean, The significant effects of maternal and embryo dominance effects in variance show that the embryo heterosis and maternal heterosis are existent and uninfluenced by environment interaction effects.
基金Supported by Joint Fund Project of Guizhou Provincial Department of Science and Technology(Qian Ke He LH Zi[2015]7716)Open Fund Project for Development Engineering Research Center of National Medicinal Plant Resources of Colleges and Universities in Guizhou Province(Qian Jiao He KY Zi[2014]227)
文摘Acanthopanax trifoliatus( L.) Merr is a common traditional Chinese herbal medicine in central and southern regions of China. It has high medicinal and edible value and mainly contains flavonoids,polysaccharides,saponins and volatile components. Its active extracts have antioxidant,anti-inflammatory,anti-fatigue,and analgesic effects. From the biological characteristics,effective chemical components,extraction methods,cultivation techniques,pharmacological effects,toxicological activities,and product development status,this paper reviewed in detail the research status of A. trifoliatus in China and abroad,to provide a scientific basis for in-depth research and development,and utilization of A. trifoliatus.
基金Supported by National Natural Science Foundation of China(81060344,81660701)Guangxi Natural Science Foundation(2016GXNSFAA380148)+2 种基金Screening and Development of Medicinal Materials Containing Stepholidine(H14002)Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica(17-259-20)Laboratory of Chemical and Quality Analysis of Chinese(Zhuang) Medicine in the Research and Development Laboratory(Level 3) of the State Administration of Traditional Chinese Medicine(Guo Zhong Yi Yao Fa 2009[21])
文摘[Objectives] This study aimed to investigate the hypolipidemic effects of different processed products of Ilex hainanensis Merr to provide experimental basis for the research on the hypolipidemic mechanism of I. hainanensis Merr. [Methods] SD rats were fed high-fat diet to establish hyperlipidemia rat models. The hyperlipidemia rats were administered with different processed products of I. hainanensis Merr by gavage to investigate the preventive and therapeutic effects of the medicinal material. [Results] The levels of total cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterol(LDL-C) in the rats fed high-fat diet were significantly higher than those in of the control group(P<0.01), indicating successful modeling. Among different processing methods, the hypolipidemic effects were in the order as baking=traditional processing=frying=microwaving>drying in shade. Among different baking times, the hypolipidemic effects ranked as 180 ℃>200 ℃=220 ℃. Among different baking times, the hypolipidemic effects ranked as 20 min=40 min>30 min. The hypolipidemic effects of processed product of I. hainanensis Merr with different amounts of active ingredients were different. [Conclusions] The processed products of I. hainanensis Merr all had hypolipidemic effects, but the effects were different. This study will provide a scientific basis for expanding the resources of I. hainanensis Merr.
基金Supported by Harbin Science and Technology Bureau(2016RQYXJ018,2017RAQXJ104)the Key Laboratory of Soybean Biology in the Chinese Ministry of Education,Northeast Agricultural University(SB17A01)+3 种基金the National Natural Science Foundation of China(31801386)Heilongjiang Natural Science Foundation(LC2018008)Heilongjiang General Young Innovative Talents Training Plan(UNPYSCT-2018158)Certificate of China Postdoctoral Science Foundation Grant(2018M641839)
文摘A survey of petal-specific proteomes of soybean(Glycine max(L.) Merr[Non-italic].) was conducted comparing protein expression profiles in different petals. Two-dimensional polyacrylamide gel electrophoresis reference maps of protein extracts from standard petals(SP), lateral wings(LW), keel petals(KP), and reproductive organs(RO)(a mixture of stamen and carpel) were obtained. Protein expression in the three petal types was compared using Image Master TM 2 D platinum 6.0 software. This indicated that the proportion of homologous proteins between SP and LW was 59.27%, between SP and KP was 61.48%, and between LW and KP was 60.05%. Within a mass range of 6.5-200.0 ku and pH 4.0-7.0, approximately 590, 646, 544, and 700 protein spots were detected in SP, LW, KP, and RO, respectively. A total of 82 differentially expressed proteins were detected. Sixty-four of these detected spots were differentially expressed and showed more than 2-fold changes in abundance; of these 64 proteins, 26 showed increased expression and 38 showed decreased expression. Among these spots, single organ-specific proteins were also identified.They were ID 49(60.9 ku), ID 45(50.0 ku), and ID 46(40.5 ku) in RO, ID 98(42.0 ku) in SP, and ID 05(29.0 ku) in KP. A total of 14 protein spots from 82 differentially expressed proteins were identified with LC-MS/MS. Further protein identification was conducted using the SwissProt and NCBInr databases. The identified proteins and their putative functions were discussed further. This was the first study reporting the comparison of petal protein profiles of soybean florets using proteomics tools.
基金Supported by Project for Improving Basic Research Ability of Middle Aged and Young Teachers in Colleges and Universities of Guangxi in 2019(2019KY0324)Program of Collaborative Innovation Center of Zhuang and Yao Medicine(zyx[2015]-01)Project of Guangxi University of Chinese Medicine(P2010016).
文摘[Objectives]To establish a method for determining the naringin content in the peel of Guangxi Citrus maxima(Burm.)Merr.[Methods]The high performance liquid chromatography(HPLC)method was applied.The chromatographic column was Agilent HC-C18(4.6 mm×250 mm,5μm);the mobile phase was acetonitrile-0.1%phosphoric acid(18∶82);the flow rate was 1.0 mL/min;the column temperature was 25℃;the detection wavelength was 283 nm.[Results]Naringin showed a good linear relationship in the range of 0.164-3.27μg,r=0.9999.The average recovery rate was 98.66%,and RSD=1.80%(n=6).[Conclusions]This method is simple,feasible,reproducible,and accurate,so it can be used for the determination of naringin content in the peel of Guangxi C.maxima.
